##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938570_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2587360 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7741431420444 31.0 31.0 34.0 30.0 34.0 2 31.997288355698473 31.0 31.0 34.0 30.0 34.0 3 32.054470580050705 33.0 31.0 34.0 30.0 34.0 4 35.741831055593345 37.0 35.0 37.0 33.0 37.0 5 35.60620748562241 37.0 35.0 37.0 33.0 37.0 6 35.63890799888689 37.0 35.0 37.0 33.0 37.0 7 35.57673420011131 37.0 35.0 37.0 33.0 37.0 8 35.56543194607631 37.0 35.0 37.0 33.0 37.0 9 37.13656468369303 39.0 37.0 39.0 33.0 39.0 10-11 37.04857828056397 39.0 37.0 39.0 33.0 39.0 12-13 36.944887452847695 39.0 37.0 39.0 33.0 39.0 14-15 37.978077847690315 40.0 37.0 41.0 33.0 41.0 16-17 37.90932572197143 40.0 37.0 41.0 33.0 41.0 18-19 38.03746598849793 40.0 37.0 41.0 33.0 41.0 20-21 38.08634418867108 40.0 37.0 41.0 33.0 41.0 22-23 37.98848072939212 40.0 37.0 41.0 33.0 41.0 24-25 37.88396724846948 40.0 37.0 41.0 32.0 41.0 26-27 37.68688605373817 40.0 37.0 41.0 32.0 41.0 28-29 37.566113528847936 39.5 36.5 41.0 32.0 41.0 30-31 37.30558097829447 39.0 36.0 41.0 31.5 41.0 32-33 37.07119129150949 39.0 36.0 41.0 31.0 41.0 34-35 36.90539565425762 39.0 35.0 40.5 30.5 41.0 36-37 36.87710639416239 39.0 35.0 41.0 30.5 41.0 38-39 36.74125981695628 39.0 35.0 41.0 30.0 41.0 40-41 36.55453918279636 39.0 35.0 41.0 30.0 41.0 42-43 36.30675147640838 39.0 35.0 40.0 30.0 41.0 44-45 36.00638565951394 38.5 35.0 40.0 29.5 41.0 46-47 35.596264338940074 38.0 35.0 40.0 27.5 41.0 48-49 35.19990994681838 38.0 34.0 40.0 26.5 41.0 50-51 34.81762027703914 38.0 34.0 40.0 26.0 41.0 52-53 34.31639856842496 38.0 33.0 40.0 24.0 41.0 54-55 33.99274337548698 37.0 33.0 40.0 23.5 41.0 56-57 33.6527901026529 37.0 33.0 40.0 23.0 41.0 58-59 33.20650682549008 36.5 32.0 39.0 22.0 41.0 60-61 32.834201657287736 36.0 31.5 39.0 21.0 40.0 62-63 32.3833735931606 35.5 31.0 39.0 19.5 40.0 64-65 31.890218407952506 35.0 31.0 38.0 18.0 40.0 66-67 31.411881802300414 35.0 30.0 38.0 16.0 40.0 68-69 30.87976721445798 34.0 29.5 37.5 12.5 39.5 70-71 30.311231912064805 34.0 29.0 36.5 8.0 39.0 72-73 29.887039878486178 34.0 29.0 36.0 7.5 39.0 74-75 29.395907024921158 34.0 28.0 36.0 7.0 38.5 76-77 28.142992857584566 31.5 26.5 34.5 3.5 36.5 78-79 28.85510578350133 33.0 28.0 35.0 2.0 37.0 80-81 28.60869109826232 33.0 27.5 35.0 2.0 36.5 82-83 28.164915203141426 33.0 27.0 35.0 2.0 36.0 84-85 27.66745060602313 33.0 26.0 35.0 2.0 36.0 86-87 27.129403909776762 32.5 25.5 35.0 2.0 35.0 88-89 26.500571045389897 32.0 25.0 34.0 2.0 35.0 90-91 25.98576000247356 32.0 24.0 34.0 2.0 35.0 92-93 25.282083668295094 31.0 20.5 34.0 2.0 35.0 94-95 24.536152294230412 31.0 18.5 34.0 2.0 35.0 96-97 23.55504317141797 31.0 4.5 34.0 2.0 35.0 98-99 22.33577043782079 30.0 2.0 34.0 2.0 35.0 100-101 18.674110676519696 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2879045149983632 1101 2 0.27294134676961335 1101 3 0.251028716852133 1101 4 0.17991675253996675 1101 5 0.0635984756924941 1101 6 0.1722940535364188 1101 7 0.2675822367520979 1101 8 0.15612946611540224 1101 9 0.17591505931412854 1101 10-11 0.2684170725341417 1101 12-13 0.33921994038046677 1101 14-15 0.36410750661901403 1101 16-17 0.4830720086458271 1101 18-19 0.3957362759259482 1101 20-21 0.43025056130287 1101 22-23 0.34263877848209745 1101 24-25 0.3672943631374608 1101 26-27 0.4188531121548138 1101 28-29 0.4172752874095593 1101 30-31 0.28003641165630455 1101 32-33 0.16828096036066853 1101 34-35 0.16161457360413323 1101 36-37 0.38620456622665245 1101 38-39 0.30451283621113134 1101 40-41 0.39965982109117704 1101 42-43 0.5275500907181154 1101 44-45 0.46327225649334736 1101 46-47 0.4375493420826615 1101 48-49 0.5574227909763962 1101 50-51 0.6131235695450172 1101 52-53 0.6429571040161264 1101 54-55 0.6003168109457917 1101 56-57 0.5965547183720119 1101 58-59 0.6600295613313989 1101 60-61 0.6533240312064308 1101 62-63 0.5505933604560056 1101 64-65 0.5149659910648339 1101 66-67 0.5874307170402808 1101 68-69 0.6019277345927989 1101 70-71 0.377738508285308 1101 72-73 0.3406681393775095 1101 74-75 0.41245794541044845 1101 76-77 0.5613253385580634 1101 78-79 0.3910515914112622 1101 80-81 0.4678559100483284 1101 82-83 0.6627134982339129 1101 84-85 0.49440645084085943 1101 86-87 0.7536877986735782 1101 88-89 0.9549001399862291 1101 90-91 0.9278918707363424 1101 92-93 0.6953246896946013 1101 94-95 0.8068299551717679 1101 96-97 0.7972422455718835 1101 98-99 0.6254411707184993 1101 100-101 0.46113941042160533 1102 1 0.25630790518904334 1102 2 0.26345012056346917 1102 3 0.3230290764673427 1102 4 0.21934529127624813 1102 5 0.14238526473730673 1102 6 0.22255371206542662 1102 7 0.29212760956681905 1102 8 0.2810820214266698 1102 9 0.3561229822910903 1102 10-11 0.36422395916545014 1102 12-13 0.37069395409488237 1102 14-15 0.4341727277414975 1102 16-17 0.470313187856803 1102 18-19 0.4401208843948865 1102 20-21 0.4261852747957988 1102 22-23 0.43514243593226354 1102 24-25 0.48981093404294995 1102 26-27 0.3166240699572427 1102 28-29 0.378574152169719 1102 30-31 0.3630209727214222 1102 32-33 0.19924064259158314 1102 34-35 0.31159414092164894 1102 36-37 0.495178763835213 1102 38-39 0.46984413172493333 1102 40-41 0.42584708253960457 1102 42-43 0.4453169619840409 1102 44-45 0.4212856276407635 1102 46-47 0.22152611420921176 1102 48-49 0.46281309607721255 1102 50-51 0.48709887051842315 1102 52-53 0.42040702351135195 1102 54-55 0.2886964174940587 1102 56-57 0.2992783132164405 1102 58-59 0.38072925811039227 1102 60-61 0.48713897648770654 1102 62-63 0.49528086301009466 1102 64-65 0.45575142337952457 1102 66-67 0.4690920575877122 1102 68-69 0.5252611224132906 1102 70-71 0.5412778575452357 1102 72-73 0.3027825950917169 1102 74-75 0.25844463964761744 1102 76-77 0.47545846157098737 1102 78-79 0.3678081073937065 1102 80-81 0.4019188263642306 1102 82-83 0.3258433765368558 1102 84-85 0.381640110824673 1102 86-87 0.28421880322597914 1102 88-89 0.41821914319825026 1102 90-91 0.416885765450818 1102 92-93 0.38565618953522574 1102 94-95 0.36655615722647994 1102 96-97 0.31071678236297373 1102 98-99 0.4424493356184236 1102 100-101 0.34031875216230745 1103 1 0.28874730398036164 1103 2 0.37405357162025865 1103 3 0.31989604374359715 1103 4 0.1975904624954623 1103 5 0.17235915088594567 1103 6 0.13424986926153082 1103 7 0.28437386499474826 1103 8 0.2352217238436367 1103 9 0.344338939671772 1103 10-11 0.3798648893818992 1103 12-13 0.3093165684226449 1103 14-15 0.47906400395272186 1103 16-17 0.3138743020582808 1103 18-19 0.36517735687699826 1103 20-21 0.32678237638646124 1103 22-23 0.3491701926194537 1103 24-25 0.4282337801616336 1103 26-27 0.4054370502487217 1103 28-29 0.42660530625403226 1103 30-31 0.570540802017554 1103 32-33 0.5918072824808647 1103 34-35 0.5008772334814395 1103 36-37 0.5362803450647107 1103 38-39 0.5257774206303623 1103 40-41 0.5972312897572252 1103 42-43 0.559766521494744 1103 44-45 0.6205823082154112 1103 46-47 0.5957615228292212 1103 48-49 0.624129819563862 1103 50-51 0.6228384967732694 1103 52-53 0.8084320248292443 1103 54-55 0.7267179175574014 1103 56-57 0.7486809362461955 1103 58-59 0.7143854412079094 1103 60-61 0.7486457905584984 1103 62-63 0.6772542802266628 1103 64-65 0.7180669092317089 1103 66-67 0.6644552950443696 1103 68-69 0.6450162234264489 1103 70-71 0.6034876763218726 1103 72-73 0.48215524895611495 1103 74-75 0.7071762806816899 1103 76-77 0.5210426714637215 1103 78-79 0.5145001299622436 1103 80-81 0.5252754200920293 1103 82-83 0.5005712425104072 1103 84-85 0.3428086801019141 1103 86-87 0.6057875540963771 1103 88-89 0.6334330777192818 1103 90-91 0.5263718835666218 1103 92-93 0.42677130370366356 1103 94-95 0.3679004816141749 1103 96-97 0.3463361070850546 1103 98-99 0.36363530888457163 1103 100-101 -0.31783456816786426 1104 1 0.29885250709842737 1104 2 0.24121652780976888 1104 3 0.27116038242158425 1104 4 0.18541379006465775 1104 5 0.258891431584523 1104 6 0.22100592538193098 1104 7 0.17882867080958675 1104 8 0.17979196337236658 1104 9 0.323681133485735 1104 10-11 0.3444407723366467 1104 12-13 0.3678539100973808 1104 14-15 0.4961812393379077 1104 16-17 0.49088526297981616 1104 18-19 0.49113017542123316 1104 20-21 0.4768053122354985 1104 22-23 0.49529403413274053 1104 24-25 0.5339346966902383 1104 26-27 0.49498433883903914 1104 28-29 0.6491602994789005 1104 30-31 0.5780208312305817 1104 32-33 0.619441811798012 1104 34-35 0.454828310711882 1104 36-37 0.45773298404539275 1104 38-39 0.4450263319320058 1104 40-41 0.5066841792215229 1104 42-43 0.6149584734260358 1104 44-45 0.6082173958394037 1104 46-47 0.36665072457113723 1104 48-49 0.40075283267049144 1104 50-51 0.5642152965583946 1104 52-53 0.41235473403587974 1104 54-55 0.4863196636997955 1104 56-57 0.6673984752295965 1104 58-59 0.491773032913585 1104 60-61 0.3698732960736635 1104 62-63 0.493653487732729 1104 64-65 0.4016194911745572 1104 66-67 0.32366947060622664 1104 68-69 0.34640655838038015 1104 70-71 0.29889167923110804 1104 72-73 0.2096795503935276 1104 74-75 0.23615006742054234 1104 76-77 0.18446995124184795 1104 78-79 0.1958486069916887 1104 80-81 0.1929162567676599 1104 82-83 0.2137163509879194 1104 84-85 0.35967965689747317 1104 86-87 0.42161955202007917 1104 88-89 0.4321998985993538 1104 90-91 0.19855349022284585 1104 92-93 0.37465758414269956 1104 94-95 0.21461907081802778 1104 96-97 0.27071163332698944 1104 98-99 0.1170145742734725 1104 100-101 -0.2433547743490756 1105 1 0.216144330054167 1105 2 0.1932849020461873 1105 3 0.13125040492721496 1105 4 0.18586926107526835 1105 5 0.12072296089542789 1105 6 0.14380805448563194 1105 7 0.13048622805604282 1105 8 0.14329176689467715 1105 9 0.20891136961619594 1105 10-11 0.18995572250275927 1105 12-13 0.3134787091542961 1105 14-15 0.3171275473434818 1105 16-17 0.22693088152730923 1105 18-19 0.3628391740994701 1105 20-21 0.26341267836274085 1105 22-23 0.24019454497474158 1105 24-25 0.2812108014307029 1105 26-27 0.32162849987930286 1105 28-29 0.22802823074743372 1105 30-31 0.3210324769991715 1105 32-33 0.3138386252870973 1105 34-35 0.3348742150451045 1105 36-37 0.4541919202080109 1105 38-39 0.38442189510851676 1105 40-41 0.4087311195280279 1105 42-43 0.3286458238350676 1105 44-45 0.21388220489881604 1105 46-47 0.14723426331296707 1105 48-49 0.11726678258654033 1105 50-51 0.016475683083605475 1105 52-53 0.09383313935148863 1105 54-55 -0.011505685494640261 1105 56-57 0.168787215271756 1105 58-59 0.3355241492641028 1105 60-61 0.12472718811810779 1105 62-63 0.3147918080270813 1105 64-65 0.11422621428390656 1105 66-67 0.11362588828207265 1105 68-69 0.24359514124038384 1105 70-71 0.2390597569648243 1105 72-73 0.2739322207039656 1105 74-75 0.27173485647495 1105 76-77 0.21445959150905836 1105 78-79 0.20049262513908062 1105 80-81 0.4212501259492427 1105 82-83 0.34474910917746726 1105 84-85 0.30561256869685494 1105 86-87 0.5750909555852566 1105 88-89 0.5081876455360899 1105 90-91 0.4304284232418176 1105 92-93 0.16118457303278078 1105 94-95 0.2270462847643735 1105 96-97 0.32193872581439464 1105 98-99 0.31359050397017896 1105 100-101 -0.22091166357138192 1106 1 0.12812862586286755 1106 2 0.1497626535416572 1106 3 0.11834101046984813 1106 4 0.06581509065505742 1106 5 0.04664400159516191 1106 6 0.020029667675970586 1106 7 0.0884667709371243 1106 8 0.13805552035672974 1106 9 0.0969446700974359 1106 10-11 0.08523946196736887 1106 12-13 0.06168191190647576 1106 14-15 0.18713516861219404 1106 16-17 0.021978520852826477 1106 18-19 0.25946658502571296 1106 20-21 0.14145610452811752 1106 22-23 0.16381563118603992 1106 24-25 0.16340598550523566 1106 26-27 0.11753605482790874 1106 28-29 0.2725126608735309 1106 30-31 0.34284754141324214 1106 32-33 0.360583780693041 1106 34-35 0.16442678602225413 1106 36-37 -0.12377648720267587 1106 38-39 0.13599554069876518 1106 40-41 0.4813568924926841 1106 42-43 0.4401376509411463 1106 44-45 0.3257856544254665 1106 46-47 0.2159995126017975 1106 48-49 0.13938132505315082 1106 50-51 0.1255741731286193 1106 52-53 0.16426008674768156 1106 54-55 -0.10583757653864012 1106 56-57 0.17653306514839784 1106 58-59 0.2651868178679706 1106 60-61 0.006740368563654897 1106 62-63 -0.14754175565940386 1106 64-65 -0.456169179183636 1106 66-67 -0.2299075604457812 1106 68-69 -0.9470350759956254 1106 70-71 -0.7674880385668565 1106 72-73 -0.48790924383041556 1106 74-75 -0.011029847584733687 1106 76-77 -0.06574498028565401 1106 78-79 -0.14680419811940482 1106 80-81 -0.7264059856914002 1106 82-83 -0.18930674753017485 1106 84-85 -0.11755695521121723 1106 86-87 -0.09907244600784892 1106 88-89 0.18005414853925572 1106 90-91 0.08218029167500873 1106 92-93 -0.19628306215945557 1106 94-95 -0.1516023514378766 1106 96-97 -0.042948844126595276 1106 98-99 -0.1624167841345283 1106 100-101 -0.6157333227565438 1107 1 0.18436224094588383 1107 2 0.23305796484541474 1107 3 0.212792565248634 1107 4 0.17831325399769327 1107 5 0.15703870892864558 1107 6 0.16287714391542352 1107 7 0.09943354870544852 1107 8 0.176668824715982 1107 9 0.2196614149992584 1107 10-11 0.16217800547874361 1107 12-13 0.15012373046793215 1107 14-15 0.2779631917502101 1107 16-17 0.26100932643008434 1107 18-19 0.3258820485206968 1107 20-21 0.3323278207780902 1107 22-23 0.19697551317597117 1107 24-25 0.28127395497419627 1107 26-27 0.3055367856242057 1107 28-29 0.3800668325990628 1107 30-31 0.32110055159799344 1107 32-33 0.38401711196416244 1107 34-35 0.3220008709802258 1107 36-37 0.3458838665182 1107 38-39 0.3839370344618658 1107 40-41 0.31395282400662694 1107 42-43 0.37792909946447395 1107 44-45 0.2717197783592411 1107 46-47 0.4188467181759563 1107 48-49 0.34534553150118086 1107 50-51 0.3607547648802907 1107 52-53 0.5075124976021286 1107 54-55 0.4546827001586209 1107 56-57 0.3012996163311996 1107 58-59 0.2405543426725103 1107 60-61 0.3182219903901 1107 62-63 0.47344321468341377 1107 64-65 0.39512634091906307 1107 66-67 0.34788261208401394 1107 68-69 0.3881084926102929 1107 70-71 0.3170387998304953 1107 72-73 0.24821915978567333 1107 74-75 0.2710512398710918 1107 76-77 0.2973457467213301 1107 78-79 0.4899291424316701 1107 80-81 0.6038617409521159 1107 82-83 0.5055123320531543 1107 84-85 0.6843116986542697 1107 86-87 0.8050289065452993 1107 88-89 0.7180415394031385 1107 90-91 0.7020391010570428 1107 92-93 0.49187716782870794 1107 94-95 0.3374932204778922 1107 96-97 0.47424661874972784 1107 98-99 0.6061991882112103 1107 100-101 0.24413503724376184 1108 1 0.12171812787520864 1108 2 0.18525092633914397 1108 3 0.0837345642184033 1108 4 -0.004537356972690532 1108 5 0.0598588377608138 1108 6 0.029977411032419354 1108 7 0.04053491509119311 1108 8 0.14980405070333802 1108 9 0.1390190331440948 1108 10-11 0.18468486927032046 1108 12-13 0.19835826237593324 1108 14-15 0.3549193723489097 1108 16-17 0.22943608433074303 1108 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-0.7258696004189531 2316 64-65 -0.5246333624887427 2316 66-67 -0.6818634536666757 2316 68-69 -0.653020587277382 2316 70-71 -0.839104051943206 2316 72-73 -0.7226644422739348 2316 74-75 -0.3749592756216096 2316 76-77 -0.4556374907725633 2316 78-79 -0.3929484837569248 2316 80-81 -0.5673378359583623 2316 82-83 -0.4385767637272231 2316 84-85 -0.3489543188813933 2316 86-87 -0.2695752524032038 2316 88-89 -0.14286247887346804 2316 90-91 -0.36327849796362344 2316 92-93 -0.11120479502746505 2316 94-95 -0.08129647772182835 2316 96-97 0.08998287773635383 2316 98-99 0.10419112373838146 2316 100-101 0.14465389908999882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 44.0 5 109.0 6 204.0 7 558.0 8 1340.0 9 2253.0 10 3707.0 11 5829.0 12 8252.0 13 11057.0 14 14493.0 15 18783.0 16 24164.0 17 30295.0 18 27825.0 19 22723.0 20 21984.0 21 22700.0 22 24750.0 23 28087.0 24 32460.0 25 38129.0 26 45516.0 27 55038.0 28 66218.0 29 79434.0 30 94211.0 31 112928.0 32 136422.0 33 169135.0 34 216669.0 35 285193.0 36 372010.0 37 410075.0 38 196767.0 39 7977.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.402948813185404 19.324435154787956 15.452243341246202 22.820372690780435 2 15.932198802052518 20.731877080844054 38.98433542529456 24.35158869180887 3 19.11569240164107 24.46270003691057 30.27207532074509 26.149532240703273 4 13.406058685300849 17.085291571331396 36.19078906684806 33.31786067651969 5 13.729895588779712 37.85221040692562 33.679359181019294 14.738534823275373 6 32.30941964009647 36.198596252550864 16.846360769278338 14.645623338074332 7 28.795876878362503 33.28640003710346 19.90387885721353 18.01384422732051 8 27.397888194916824 34.198681281306044 19.298048976562985 19.105381547214147 9 27.024496011378396 16.39354399851586 19.538912250324657 37.04304773978109 10-11 25.257308608001978 26.588105250139137 26.778917506647705 21.37566863521118 12-13 26.327762661554633 23.867088460824935 27.195848277781216 22.60930059983922 14-15 22.948159513944717 25.194522602189107 25.7036515985406 26.153666285325585 16-17 22.26924355327438 28.393942087687833 26.616068115762786 22.720746243275 18-19 22.421696246366952 26.480447251252244 29.258665203141426 21.83919129923938 20-21 23.06414646589574 25.55363381980088 29.465033856904334 21.917185857399048 22-23 22.723181157627852 25.51940974584132 29.54496088677262 22.21244820975821 24-25 22.544909305976166 25.865715682066636 29.520144812063755 22.069230199893443 26-27 21.860713032265647 26.236342103350875 29.362158639552128 22.540786224831358 28-29 22.131670892338136 26.56379475604477 29.166138457732977 22.138395893884113 30-31 22.2739587842434 26.060482499536207 28.979732236720057 22.68582647950034 32-33 21.849568672314636 26.13194530332076 28.915477552408632 23.10300847195597 34-35 22.7984212426433 26.230857895603492 28.68588665755994 22.28483420419327 36-37 23.00110614851609 25.6010189552578 28.829275909461245 22.56859898676487 38-39 22.274214192358436 26.113335795398875 28.716999093828466 22.89545091841422 40-41 22.740607436593457 25.945827066986237 28.89544544306601 22.418120053354297 42-43 22.519538323173006 26.040340100743965 28.53895741242684 22.901164163656187 44-45 22.45236457238266 26.20383325088121 28.789731618329107 22.554070558407023 46-47 22.99548574608868 26.535174850040192 27.852386216065796 22.61695318780533 48-49 22.553084997835633 26.77999969080453 27.665844722033267 23.001070589326574 50-51 22.885508781151444 26.784869519510234 27.63915728773731 22.690464411601013 52-53 23.252562457485624 26.43410657967967 27.66955506771381 22.643775895120896 54-55 22.571308206047863 26.79385551295529 27.78985143157504 22.844984849421802 56-57 22.706445952631256 26.9802810586853 27.400864201348092 22.91240878733535 58-59 22.56267005751036 26.43317899325954 27.651447807804093 23.35270314142601 60-61 22.15619395832045 26.805141147733597 27.982325613753016 23.05633928019294 62-63 22.341537319893636 26.737118143590376 28.080939645043596 22.84040489147239 64-65 22.084634530950467 26.82400207160967 27.724495238389714 23.366868159050153 66-67 22.007477128204574 26.896906766248335 27.649141575746988 23.446474529800103 68-69 22.739100430809497 26.399367535403663 27.694462862126468 23.167069171660376 70-71 22.504056261251826 26.528972797734212 27.347491147340257 23.6194797936737 72-73 22.404922391936182 26.64176998948735 27.370099251746954 23.58320836682951 74-75 22.33680276420753 27.130472759878792 27.159421186073835 23.373303289839836 76-77 22.381462185393605 26.60431868777441 27.526069043349207 23.48815008348278 78-79 22.476133974398614 26.85979144765321 27.411067651969574 23.253006925978603 80-81 22.70001830079354 26.8218130772021 27.102045186147283 23.37612343585707 82-83 22.583111886510494 26.87908684612636 27.21106866297652 23.326732604386628 84-85 22.387981821372332 27.026794072697502 27.128795685242892 23.456428420687274 86-87 22.648938715388965 26.945173113633615 26.807384099727553 23.598504071249867 88-89 22.425563533450635 26.96198727852645 26.642626695383225 23.969822492639693 90-91 22.1965022564342 26.96079416589236 26.917273355524053 23.925430222149387 92-93 22.39532414175604 26.57020309527283 27.07336610196022 23.961106661010906 94-95 22.08188389819192 26.35937385561375 27.299077418146734 24.2596648280476 96-97 22.42876909282048 25.87828906684806 27.294597582091395 24.39834425824006 98-99 22.06594526153706 26.262562042052163 26.858227581958122 24.81326511445266 100-101 22.12036593284274 25.974294261332016 27.29432703605219 24.61101276977305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 552.0 1 388.0 2 307.0 3 426.5 4 381.0 5 339.5 6 650.0 7 1521.0 8 2366.5 9 2642.5 10 2344.5 11 1974.5 12 1975.0 13 2138.0 14 2378.0 15 2610.0 16 2835.5 17 3121.5 18 3504.5 19 3880.0 20 4262.5 21 4902.0 22 5851.0 23 7019.0 24 8473.5 25 10582.5 26 13811.0 27 17576.5 28 20403.0 29 23535.5 30 27766.5 31 34030.5 32 44119.5 33 48080.0 34 47597.5 35 50509.5 36 55512.5 37 60650.0 38 64323.0 39 66796.0 40 69105.0 41 74298.5 42 78325.5 43 80921.0 44 83981.0 45 88932.0 46 91828.5 47 92975.5 48 94660.5 49 93925.5 50 92994.0 51 92031.0 52 90588.0 53 91808.5 54 95080.5 55 91005.0 56 82958.0 57 75596.0 58 70465.0 59 63788.5 60 52316.0 61 44348.0 62 38042.0 63 27944.5 64 18991.5 65 14274.0 66 10549.0 67 8251.0 68 6623.0 69 5073.5 70 3773.0 71 2534.5 72 1901.5 73 1610.5 74 1215.5 75 883.0 76 639.5 77 443.5 78 316.5 79 202.5 80 125.5 81 82.5 82 46.0 83 18.5 84 13.0 85 7.0 86 3.5 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.410920784119721E-4 2 0.04908478139880032 3 9.66235854307093E-4 4 0.0 5 0.08862315255704656 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5122132211984413E-4 26-27 0.008193680044524148 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010126151753138335 36-37 1.5459773668913488E-4 38-39 0.003922917568486797 40-41 0.004908478139880032 42-43 3.4784490755055347E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1594830251685115E-4 68-69 8.309628347040999E-4 70-71 7.729886834456744E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015653020839774907 82-83 7.536639663595324E-4 84-85 0.0056428173891534235 86-87 0.002744109826232144 88-89 9.662358543070928E-5 90-91 0.0035557479438501015 92-93 0.0010628594397378021 94-95 5.7974151258425575E-5 96-97 0.0 98-99 7.729886834456744E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2587360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.48518506690473 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36627026939598 30.16405100065615 2 15.164843585816895 13.188920596798216 3 6.017305131988373 7.849908818056497 4 3.0370978355963953 5.282750457888201 5 1.6882293177324939 3.6706482158485896 6 1.0603196630273526 2.766491806609349 7 0.7200490712208675 2.1918026983504597 8 0.49863812945006747 1.7346697072441102 9 0.3547364896827888 1.3883203713456187 >10 1.8469530616194632 14.524549670234851 >50 0.15265416440861349 4.542712179873262 >100 0.08389915902567255 6.892736058424618 >500 0.004279186234487224 1.3142372796484962 >1k 0.00445748566092195 3.5885760450269215 >5k 2.6744913965531705E-4 0.8996250939946281 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9629 0.37215540164491995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7905 0.30552377713190276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5672 0.21921959062519325 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3993 0.15432719064992886 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3798 0.14679055098633356 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3743 0.14466483210685796 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3345 0.12928235730628904 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3157 0.12201626368189969 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3127 0.1208567806567312 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3074 0.11880836064560014 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2597 0.10037258054542081 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.864943417228372E-5 2 0.0 0.0 0.0 0.0 3.864943417228372E-5 3 0.0 0.0 0.0 0.0 3.864943417228372E-5 4 0.0 0.0 0.0 0.0 3.864943417228372E-5 5 0.0 0.0 0.0 0.0 3.864943417228372E-5 6 0.0 0.0 0.0 0.0 7.729886834456744E-5 7 0.0 0.0 0.0 0.0 7.729886834456744E-5 8 0.0 0.0 0.0 0.0 7.729886834456744E-5 9 0.0 0.0 0.0 0.0 7.729886834456744E-5 10-11 0.0 0.0 0.0 0.0 7.729886834456744E-5 12-13 0.0 0.0 0.0 3.864943417228372E-5 7.729886834456744E-5 14-15 0.0 0.0 0.0 7.729886834456744E-5 9.66235854307093E-5 16-17 0.0 0.0 0.0 1.1594830251685115E-4 1.1594830251685115E-4 18-19 0.0 0.0 0.0 1.1594830251685115E-4 1.1594830251685115E-4 20-21 0.0 0.0 0.0 1.5459773668913488E-4 1.1594830251685115E-4 22-23 0.0 0.0 0.0 3.0919547337826975E-4 1.3527301960299302E-4 24-25 0.0 0.0 0.0 7.536639663595324E-4 1.5459773668913488E-4 26-27 0.0 0.0 0.0 0.0015459773668913487 1.5459773668913488E-4 28-29 0.0 0.0 0.0 0.005005101725310742 1.5459773668913488E-4 30-31 0.0 0.0 0.0 0.016696555562426565 1.5459773668913488E-4 32-33 0.0 0.0 0.0 0.03969296889493538 1.5459773668913488E-4 34-35 0.0 0.0 0.0 0.07393636757157876 1.932471708614186E-4 36-37 0.0 0.0 0.0 0.132818780533053 1.932471708614186E-4 38-39 0.0 0.0 0.0 0.21769293797538802 2.318966050337023E-4 40-41 0.0 0.0 0.0 0.3093500711149589 2.318966050337023E-4 42-43 0.0 0.0 0.0 0.40481417352049964 2.318966050337023E-4 44-45 0.0 0.0 0.0 0.5233133386927216 2.318966050337023E-4 46-47 0.0 0.0 0.0 0.6653693339929503 2.318966050337023E-4 48-49 0.0 0.0 0.0 0.8190974584132089 2.7054603920598604E-4 50-51 0.0 0.0 0.0 0.988575227258673 2.7054603920598604E-4 52-53 0.0 0.0 0.0 1.1537242594768413 2.898707562921279E-4 54-55 0.0 0.0 0.0 1.3306806938346423 3.0919547337826975E-4 56-57 0.0 0.0 0.0 1.5332617030486673 3.0919547337826975E-4 58-59 0.0 0.0 0.0 1.7470123987384825 3.4784490755055347E-4 60-61 0.0 0.0 0.0 1.9815758147300724 3.864943417228372E-4 62-63 0.0 0.0 0.0 2.2178591305423287 3.864943417228372E-4 64-65 0.0 0.0 0.0 2.4658725496258738 3.864943417228372E-4 66-67 0.0 0.0 0.0 2.7543712510048852 3.864943417228372E-4 68-69 0.0 0.0 0.0 3.0726106919794693 3.864943417228372E-4 70-71 0.0 0.0 0.0 3.410793240986952 3.864943417228372E-4 72-73 0.0 0.0 0.0 3.7699431080328987 5.024426442396884E-4 74-75 0.0 0.0 0.0 4.12866783130295 5.024426442396884E-4 76-77 0.0 0.0 0.0 4.527472017809659 5.024426442396884E-4 78-79 0.0 0.0 0.0 4.964713066600705 5.024426442396884E-4 80-81 0.0 0.0 0.0 5.41658292622596 5.024426442396884E-4 82-83 0.0 0.0 0.0 5.894386556180818 5.024426442396884E-4 84-85 0.0 0.0 0.0 6.384712602807495 5.024426442396884E-4 86-87 0.0 0.0 0.0 6.886904025725063 5.410920784119721E-4 88-89 0.0 0.0 0.0 7.420826634098077 5.797415125842558E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5155 0.0 59.31895 1 GTATCAA 10050 0.0 44.90671 1 TATCAAC 13805 0.0 32.795303 2 ATCAACG 13925 0.0 32.25802 3 TCAACGC 14085 0.0 31.824085 4 CAACGCA 14415 0.0 31.095543 5 TAAGGTG 1860 0.0 30.666866 5 GTAAGGT 1755 0.0 30.605705 4 AACGCAG 14935 0.0 30.1163 6 TGGTATC 2070 0.0 30.096228 2 AGGTAAG 1680 0.0 28.873672 2 GGTAAGG 1840 0.0 28.158516 3 ACGCAGA 17040 0.0 26.25658 7 CGCAGAG 17280 0.0 25.781961 8 AAGGTAA 1890 0.0 25.162241 1 GTGGTAT 2750 0.0 24.210651 1 TACATGG 12700 0.0 23.516195 2 GTACATG 12955 0.0 23.383696 1 GCAGAGT 18955 0.0 22.97748 9 ACCGTAC 105 0.00219804 22.617132 8 >>END_MODULE