FastQCFastQC Report
Thu 26 May 2016
SRR938569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584820
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100950.390549438645631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77660.30044645275106197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.21514070612267006No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38030.14712823330057798No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37470.1449617381481109No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35130.1359088834038734No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33850.1309568944839486No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32220.12465084609373187No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30330.11733892495415542No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29150.11277381016859973No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28290.10944669261302528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.058.9346051
GTATCAA97950.044.514391
TATCAAC130250.033.3469922
ATCAACG133250.032.5248873
TCAACGC134150.032.3421064
CAACGCA137050.031.6230665
AACGCAG141500.030.590246
TAAGGTG18350.029.2637885
GTAAGGT18350.029.0048184
AGGTAAG16050.028.1278672
ACGCAGA158450.027.1337647
AAGGTAA16450.026.6505221
CGCAGAG161250.026.633148
TGGTATC21400.025.7592032
TACATGG130700.025.742212
GTACATG133900.025.6945131
GGTAAGG18950.025.0771963
ACATGGG135050.024.209293
GCAGAGT175150.024.0027839
TAAGGTA7900.023.4598754