FastQCFastQC Report
Thu 26 May 2016
SRR938569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584820
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96560.37356566414682646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.30682987596815253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.22063431883071163No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40650.15726433562104905No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37200.14391717798531425No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37080.1434529290240713No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33500.12960283501365666No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31940.12356759851749831No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30410.11764842426165072No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30340.11737761236759234No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT26490.10248295819438102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.057.6037181
GTATCAA96450.044.0865141
TGGTATC21900.032.1431242
TATCAAC132350.031.9483382
ATCAACG133000.031.776813
TCAACGC133350.031.7647084
CAACGCA135400.031.2135585
AACGCAG141050.030.002196
TAAGGTG19300.028.0806885
GTAAGGT19300.027.8343664
GTGGTAT27150.027.3290921
AGGTAAG17200.026.8233952
ACGCAGA160900.026.153277
CGCAGAG162750.025.7392548
AAGGTAA16850.025.6869131
GGTAAGG20400.024.7022133
GTACATG128850.023.8461651
TACATGG125900.023.8004552
GCAGAGT182050.022.488739
ACATGGG129550.022.0178053