##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2584820 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790075904705162 31.0 31.0 34.0 30.0 34.0 2 32.0174925139855 31.0 31.0 34.0 30.0 34.0 3 32.076780588203434 33.0 31.0 34.0 30.0 34.0 4 35.762489844553976 37.0 35.0 37.0 35.0 37.0 5 35.63220262919662 37.0 35.0 37.0 33.0 37.0 6 35.67202900008511 37.0 35.0 37.0 33.0 37.0 7 35.60642443187533 37.0 35.0 37.0 33.0 37.0 8 35.5863054293916 37.0 35.0 37.0 33.0 37.0 9 37.14690462005091 39.0 37.0 39.0 33.0 39.0 10-11 37.08891489542792 39.0 37.0 39.0 33.0 39.0 12-13 36.98154416941992 39.0 37.0 39.0 33.0 39.0 14-15 38.01638992270255 40.0 37.0 41.0 33.0 41.0 16-17 37.94245189220139 40.0 37.0 41.0 33.0 41.0 18-19 37.96714026508616 40.0 37.0 41.0 33.0 41.0 20-21 37.96232929178821 40.0 37.0 41.0 33.0 41.0 22-23 37.9841397079874 40.0 37.0 41.0 33.0 41.0 24-25 37.90104127173265 40.0 37.0 41.0 32.5 41.0 26-27 37.71869472535805 40.0 37.0 41.0 32.0 41.0 28-29 37.58877175199821 39.5 37.0 41.0 32.0 41.0 30-31 37.322826541113116 39.0 36.0 41.0 31.5 41.0 32-33 37.0795689835269 39.0 36.0 41.0 31.0 41.0 34-35 36.900168677122586 39.0 35.0 40.5 31.0 41.0 36-37 36.88609052080996 39.0 35.0 41.0 31.0 41.0 38-39 36.72424501512678 39.0 35.0 41.0 30.0 41.0 40-41 36.53403486509699 39.0 35.0 40.5 30.0 41.0 42-43 36.28294001129673 39.0 35.0 40.0 30.0 41.0 44-45 35.9645764501977 38.0 35.0 40.0 29.5 41.0 46-47 35.56372223210901 38.0 34.5 40.0 27.5 41.0 48-49 35.127606177606175 38.0 34.0 40.0 26.5 41.0 50-51 34.73808872571398 38.0 34.0 40.0 25.5 41.0 52-53 34.215818703043155 38.0 33.0 40.0 24.0 41.0 54-55 33.880909695839556 37.0 33.0 40.0 23.0 41.0 56-57 33.513235351010906 37.0 32.5 40.0 23.0 41.0 58-59 33.04944154718704 36.0 31.5 39.0 21.5 41.0 60-61 32.64266119110809 36.0 31.0 39.0 20.0 40.0 62-63 32.17015169334809 35.0 31.0 39.0 18.5 40.0 64-65 31.677005555512572 35.0 30.5 38.0 17.0 40.0 66-67 31.18876130639658 35.0 30.0 38.0 13.5 40.0 68-69 30.632753731401024 34.0 29.0 37.0 9.0 39.5 70-71 30.078638357796674 34.0 29.0 36.5 7.5 39.0 72-73 29.50574933651086 34.0 28.0 36.0 6.0 39.0 74-75 28.91532137634342 33.5 27.0 35.5 2.0 38.0 76-77 27.64619431914021 31.5 26.0 34.5 2.0 36.0 78-79 28.293663195116103 33.0 27.0 35.0 2.0 37.0 80-81 28.02043585239978 33.0 26.0 35.0 2.0 36.5 82-83 27.548407045751738 33.0 26.0 35.0 2.0 36.0 84-85 26.985863812567217 32.5 25.0 35.0 2.0 36.0 86-87 26.377994599237084 32.0 24.5 34.5 2.0 35.0 88-89 25.62510639038695 31.5 22.0 34.0 2.0 35.0 90-91 25.094614518612516 31.0 20.0 34.0 2.0 35.0 92-93 24.337779806717684 31.0 17.0 34.0 2.0 35.0 94-95 23.572215086543743 31.0 4.5 34.0 2.0 35.0 96-97 22.60285493767458 30.0 2.0 34.0 2.0 35.0 98-99 21.423356752114266 29.5 2.0 34.0 2.0 35.0 100-101 18.266719926339164 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3083315888699154 1101 2 0.3525891278277342 1101 3 0.2797626373791644 1101 4 0.2163724635483959 1101 5 0.17557056437495078 1101 6 0.22330792240012443 1101 7 0.16740536374724968 1101 8 0.14607616582490124 1101 9 0.25495418041496976 1101 10-11 0.2800417617820372 1101 12-13 0.3931136189193367 1101 14-15 0.4873069550466127 1101 16-17 0.5296488419881129 1101 18-19 0.4626882348378416 1101 20-21 0.577403303146987 1101 22-23 0.6337171787319278 1101 24-25 0.5438126363386289 1101 26-27 0.4589345314431412 1101 28-29 0.4993037418164121 1101 30-31 0.4241370278730585 1101 32-33 0.4407569815094661 1101 34-35 0.32400733568734097 1101 36-37 0.5499360130869277 1101 38-39 0.6374167906316544 1101 40-41 0.8014044878076092 1101 42-43 0.7488977599151809 1101 44-45 0.7790828446608415 1101 46-47 0.8469499784427015 1101 48-49 0.9114925968288006 1101 50-51 0.930243224541222 1101 52-53 0.9575255174842567 1101 54-55 0.9167723035617357 1101 56-57 1.0897585152420888 1101 58-59 1.0629506674491154 1101 60-61 0.9506685971570406 1101 62-63 1.1260739694969075 1101 64-65 1.309636487700022 1101 66-67 1.4051061046425097 1101 68-69 1.371404725113429 1101 70-71 1.4556174546187641 1101 72-73 1.6176947832237651 1101 74-75 1.576954229424377 1101 76-77 1.3732369608797264 1101 78-79 1.5766820993034294 1101 80-81 1.98448318190502 1101 82-83 2.045870854287859 1101 84-85 2.2422451526745952 1101 86-87 2.1841746637659902 1101 88-89 2.4328506396522265 1101 90-91 2.737941029661272 1101 92-93 2.9252551329083225 1101 94-95 2.9235516767218783 1101 96-97 3.443811613509478 1101 98-99 3.4947257420953513 1101 100-101 3.954292643549852 1102 1 0.2735392411463984 1102 2 0.28681734082545773 1102 3 0.26017142802418647 1102 4 0.23129789697575376 1102 5 0.17853868460250055 1102 6 0.1468255538826 1102 7 0.2531102140334056 1102 8 0.27186932248488915 1102 9 0.32959902094540894 1102 10-11 0.35733786383932653 1102 12-13 0.2838821994630507 1102 14-15 0.4679063658661633 1102 16-17 0.3250967312294577 1102 18-19 0.4134713681588593 1102 20-21 0.3696444813462989 1102 22-23 0.3268247571841201 1102 24-25 0.27231042817433604 1102 26-27 0.3468496163410819 1102 28-29 0.33547163501660293 1102 30-31 0.3595241105411162 1102 32-33 0.328549867514063 1102 34-35 0.5015666198240041 1102 36-37 0.6387842026749766 1102 38-39 0.7060998905247047 1102 40-41 0.5887203436694719 1102 42-43 0.4883457339888224 1102 44-45 0.5473041135297194 1102 46-47 0.669764865297644 1102 48-49 0.8008914893634582 1102 50-51 0.8092239039872666 1102 52-53 1.0071673030423653 1102 54-55 0.8151765475999966 1102 56-57 1.0031677229791995 1102 58-59 1.1018147125960525 1102 60-61 1.1257970322335709 1102 62-63 1.2125041863545007 1102 64-65 1.179508552528052 1102 66-67 1.2512576979241992 1102 68-69 1.3426188064655733 1102 70-71 1.3584610026686832 1102 72-73 1.317164025964292 1102 74-75 1.49439347587127 1102 76-77 1.2769482725586059 1102 78-79 1.6507202374770493 1102 80-81 1.9361810395867352 1102 82-83 1.9008245550460003 1102 84-85 2.0371519835927323 1102 86-87 2.240279356910431 1102 88-89 2.3458541167661906 1102 90-91 2.4506691048065257 1102 92-93 2.5390942585701914 1102 94-95 2.7756135053222764 1102 96-97 3.1412893172609806 1102 98-99 3.2954961000775143 1102 100-101 3.708971043330095 1103 1 0.2408152142903468 1103 2 0.25806059770440726 1103 3 0.3070521281444911 1103 4 0.25045224168022173 1103 5 0.15805123942067922 1103 6 0.19391776485011292 1103 7 0.2038970731943479 1103 8 0.2445242687339686 1103 9 0.15080152802811142 1103 10-11 0.3499044218398737 1103 12-13 0.4694383371585573 1103 14-15 0.5290422179249958 1103 16-17 0.5892073184146156 1103 18-19 0.46592848384946706 1103 20-21 0.48572852294989843 1103 22-23 0.5250497154640925 1103 24-25 0.508178010741176 1103 26-27 0.45390709295845966 1103 28-29 0.43020375361790997 1103 30-31 0.4500575447787014 1103 32-33 0.5246582619720854 1103 34-35 0.4484880697405629 1103 36-37 0.6106901288596376 1103 38-39 0.7273552753192121 1103 40-41 0.5864121587818261 1103 42-43 0.7123168016334773 1103 44-45 0.7148546626817378 1103 46-47 0.8547131518657309 1103 48-49 1.0496888852574315 1103 50-51 0.9882018602879583 1103 52-53 1.2263599719599725 1103 54-55 1.1963564482481175 1103 56-57 1.1363928753058161 1103 58-59 1.2104177707712367 1103 60-61 1.3363017475671413 1103 62-63 1.3337324046181749 1103 64-65 1.1903897183603114 1103 66-67 1.391363259301155 1103 68-69 1.4718325294445833 1103 70-71 1.398369121580469 1103 72-73 1.3708664360236718 1103 74-75 1.62325888511565 1103 76-77 1.508999927776518 1103 78-79 1.8058760569360501 1103 80-81 2.028490970894218 1103 82-83 2.2518903562646138 1103 84-85 2.4728420134759155 1103 86-87 2.62645353318241 1103 88-89 2.723537781919294 1103 90-91 2.754649001573707 1103 92-93 3.0221137214490668 1103 94-95 3.0381862728155795 1103 96-97 3.1453317940724084 1103 98-99 3.5593175577559393 1103 100-101 4.031452317828489 1104 1 0.25599018166416343 1104 2 0.28799246210090246 1104 3 0.2602608749956872 1104 4 0.17036331637140734 1104 5 0.14457283339925198 1104 6 0.241844105558485 1104 7 0.24466386429575238 1104 8 0.26314091870816725 1104 9 0.34854728662473633 1104 10-11 0.3129674131294351 1104 12-13 0.34557949470506344 1104 14-15 0.39203312811953595 1104 16-17 0.5340656600941784 1104 18-19 0.537281559815348 1104 20-21 0.6027119670605003 1104 22-23 0.41894672296052704 1104 24-25 0.3821499067015992 1104 26-27 0.39887962626750806 1104 28-29 0.37795216783381846 1104 30-31 0.4047018435434637 1104 32-33 0.38875960040420665 1104 34-35 0.4451639931373279 1104 36-37 0.4113183725089087 1104 38-39 0.5147778829856051 1104 40-41 0.5396011781754311 1104 42-43 0.42723872642633864 1104 44-45 0.4132529448754312 1104 46-47 0.5669733873812248 1104 48-49 0.37391761754066266 1104 50-51 0.5001847732746967 1104 52-53 0.7346709222164307 1104 54-55 0.7943689068963309 1104 56-57 0.8341261145543584 1104 58-59 0.8385893389523673 1104 60-61 0.8063258757652605 1104 62-63 0.7960009863657191 1104 64-65 0.9073560906326144 1104 66-67 1.0357621788434805 1104 68-69 1.0688172634274018 1104 70-71 1.2556944577059959 1104 72-73 1.1892402865198939 1104 74-75 1.2379520472753534 1104 76-77 1.2988440808615884 1104 78-79 1.3811757625416838 1104 80-81 1.7550319888310284 1104 82-83 1.882917473931311 1104 84-85 1.9670155847527404 1104 86-87 2.3235581494074857 1104 88-89 2.3670709695729393 1104 90-91 2.5116385915460846 1104 92-93 2.8365196973512177 1104 94-95 3.006895869464948 1104 96-97 3.2644662898082437 1104 98-99 3.4583626729339407 1104 100-101 3.832463729403951 1105 1 0.3171733421007552 1105 2 0.24830701564792435 1105 3 0.24688001579395547 1105 4 0.23989947033906844 1105 5 0.191713888436027 1105 6 0.1942945319281293 1105 7 0.23983694015081625 1105 8 0.199117024986478 1105 9 0.30160371109170825 1105 10-11 0.26067761015455204 1105 12-13 0.4221604306482831 1105 14-15 0.48155063044377755 1105 16-17 0.3991971844729463 1105 18-19 0.4502345708007809 1105 20-21 0.48523986338447855 1105 22-23 0.2768110304515403 1105 24-25 0.35429837262917374 1105 26-27 0.5212898715445604 1105 28-29 0.4205785298329374 1105 30-31 0.5700588321036406 1105 32-33 0.6669159005990153 1105 34-35 0.6807967822359942 1105 36-37 0.7484225209488713 1105 38-39 0.6903083837598771 1105 40-41 0.6406625764139875 1105 42-43 0.8538750186585062 1105 44-45 0.7265634817556972 1105 46-47 0.881370133657569 1105 48-49 0.849497957006335 1105 50-51 0.9756323297723739 1105 52-53 1.0606748905591274 1105 54-55 1.1356013722959446 1105 56-57 1.2024195468822825 1105 58-59 1.2077061555077506 1105 60-61 1.3086351886765115 1105 62-63 1.3367542502230023 1105 64-65 1.2355785659350538 1105 66-67 1.2699870790038084 1105 68-69 1.3710406749182837 1105 70-71 1.3870036141050015 1105 72-73 1.4902463645322293 1105 74-75 1.7349341738656285 1105 76-77 1.517713471033634 1105 78-79 1.8127177399188135 1105 80-81 2.0289238713271196 1105 82-83 2.1814911208757515 1105 84-85 2.317000402343851 1105 86-87 2.5234304152215294 1105 88-89 2.694894951720446 1105 90-91 2.8881185518482333 1105 92-93 3.064203034855808 1105 94-95 3.181667394045668 1105 96-97 3.552396887807628 1105 98-99 3.654703171450681 1105 100-101 4.16878668700933 1106 1 0.17457295981279586 1106 2 0.21391536385329601 1106 3 0.16169731955439204 1106 4 0.13529648240681524 1106 5 0.07492586806001356 1106 6 0.16592565891683364 1106 7 0.2100701484850731 1106 8 0.07881677361471162 1106 9 0.17836497765038928 1106 10-11 0.2464210587976865 1106 12-13 0.15803225957600375 1106 14-15 0.09668397159616404 1106 16-17 0.20025370666689213 1106 18-19 0.3035070001044531 1106 20-21 0.3961637576498447 1106 22-23 0.172661335869833 1106 24-25 0.11690816947133698 1106 26-27 0.21995836195090135 1106 28-29 0.3103593006852279 1106 30-31 0.3465582662794233 1106 32-33 0.3148607065862947 1106 34-35 0.312784394331004 1106 36-37 0.46939906698033695 1106 38-39 0.46559375252827806 1106 40-41 0.4508697600041387 1106 42-43 0.5440426738005328 1106 44-45 0.03903554715673607 1106 46-47 0.0259263341083269 1106 48-49 0.4987986806907543 1106 50-51 0.39388261743930286 1106 52-53 0.7532199583787786 1106 54-55 0.6896645290561949 1106 56-57 0.7017364369729151 1106 58-59 1.0539263863386736 1106 60-61 0.9152233161945915 1106 62-63 0.9879584889913176 1106 64-65 0.6635350370938689 1106 66-67 0.9067651776442496 1106 68-69 0.8401501595269139 1106 70-71 0.9873312215719814 1106 72-73 1.3910472356162344 1106 74-75 1.7593105785202887 1106 76-77 1.7482559332089949 1106 78-79 1.787354709002937 1106 80-81 1.7680376986762418 1106 82-83 2.0389850426240024 1106 84-85 2.0512500433545355 1106 86-87 2.3423817226400097 1106 88-89 2.4571703258459543 1106 90-91 2.5561556648450754 1106 92-93 2.2281541679306898 1106 94-95 2.755409980480465 1106 96-97 3.167932804173418 1106 98-99 3.6832321235822683 1106 100-101 4.107135621452969 1107 1 0.23948348042848622 1107 2 0.16583150818616588 1107 3 0.18579523911652274 1107 4 0.11014469113170833 1107 5 0.1388975684597824 1107 6 0.07650916942947106 1107 7 0.13218642106200917 1107 8 0.14454339284947793 1107 9 0.07598755351534692 1107 10-11 0.18596533214776656 1107 12-13 0.2434334518163226 1107 14-15 0.31094667558246414 1107 16-17 0.4098667961524072 1107 18-19 0.3003699292848907 1107 20-21 0.17972811438924907 1107 22-23 0.17498691013020107 1107 24-25 0.25808325944160515 1107 26-27 0.22703249499952705 1107 28-29 0.2661342722291522 1107 30-31 0.27645969015675576 1107 32-33 0.3640613287847927 1107 34-35 0.3499767082773957 1107 36-37 0.44561945656004553 1107 38-39 0.437895488118464 1107 40-41 0.4856206811831214 1107 42-43 0.6264962466864219 1107 44-45 0.6457657529301741 1107 46-47 0.6235406262654948 1107 48-49 0.6685372223286592 1107 50-51 0.5605983727447139 1107 52-53 0.6735306894620123 1107 54-55 0.7631663284616081 1107 56-57 0.7487633528871456 1107 58-59 0.8109997523773131 1107 60-61 0.9240201536590362 1107 62-63 1.1453435678859663 1107 64-65 1.201305676565429 1107 66-67 1.196398117348064 1107 68-69 1.2977743921334266 1107 70-71 1.1094982702096168 1107 72-73 1.4606280800021842 1107 74-75 1.429769342529724 1107 76-77 1.435751522901608 1107 78-79 1.5313137383978308 1107 80-81 1.735386083813431 1107 82-83 1.8308944679494452 1107 84-85 2.0873393969492042 1107 86-87 2.333687664512926 1107 88-89 2.573366968796673 1107 90-91 2.654289989768909 1107 92-93 2.919954217632938 1107 94-95 3.0366065774105877 1107 96-97 3.257303273061961 1107 98-99 3.4516472662001085 1107 100-101 3.7567409181170888 1108 1 0.09108110599049724 1108 2 0.11180737107269323 1108 3 0.13944104290392545 1108 4 0.08361626667691269 1108 5 0.029515454709596156 1108 6 -0.005911058385080992 1108 7 0.05817505506197307 1108 8 0.0854457200139862 1108 9 0.03423561275702269 1108 10-11 0.08069892061245554 1108 12-13 0.17404688708582938 1108 14-15 0.3264509236504409 1108 16-17 0.24229029674659586 1108 18-19 0.20078724505284384 1108 20-21 0.2471030105694041 1108 22-23 0.15851319200525893 1108 24-25 0.1683000047277119 1108 26-27 0.25120160897807153 1108 28-29 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62-63 0.20927069915870788 2316 64-65 0.18329846631390367 2316 66-67 0.3950592518984912 2316 68-69 0.5102498545210956 2316 70-71 0.5221902120495407 2316 72-73 0.5651329318596758 2316 74-75 0.7957391328201169 2316 76-77 0.6982180215910283 2316 78-79 0.7568565537685679 2316 80-81 0.9757576802685506 2316 82-83 1.2494070834338054 2316 84-85 1.3792072351998819 2316 86-87 1.6218998282923813 2316 88-89 1.7557340236446812 2316 90-91 1.8676783986838181 2316 92-93 1.7814925028569952 2316 94-95 2.3303520853289257 2316 96-97 2.4971264057885456 2316 98-99 2.772351403581265 2316 100-101 3.255178205792042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 46.0 5 78.0 6 215.0 7 557.0 8 1305.0 9 2267.0 10 3644.0 11 5765.0 12 8211.0 13 11380.0 14 14744.0 15 19459.0 16 24847.0 17 31223.0 18 29149.0 19 24601.0 20 23906.0 21 25370.0 22 27358.0 23 31038.0 24 35520.0 25 42168.0 26 49526.0 27 59385.0 28 70927.0 29 84891.0 30 100552.0 31 118710.0 32 143085.0 33 174765.0 34 216896.0 35 278200.0 36 346937.0 37 374127.0 38 193277.0 39 10670.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.41095504596455 19.291454949632815 15.484146272580892 22.813443731821735 2 15.96879734141087 20.65585344008769 38.98613808654968 24.38921113195176 3 19.166973587288226 24.440230948740645 30.256621747930996 26.136173716040133 4 13.43838255661903 17.105523788890522 36.12328904914075 33.3328046053497 5 13.734333393745905 37.782525586273024 33.724001294997684 14.759139724983386 6 32.2678561756718 36.23958341393212 16.87622348944994 14.616336920946138 7 28.85713511966017 33.19418760300524 19.82594532694733 18.122731950387262 8 27.45088632864184 34.17487484621753 19.275926370114746 19.09831245502588 9 26.997895404709034 16.399826680387804 19.561787668000093 37.040490246903076 10-11 25.295223651937075 26.55271933829048 26.756369882622387 21.395687127150055 12-13 26.355374842348787 23.853189003489604 27.165953528678983 22.625482625482626 14-15 22.96061234437988 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28.67755283025292 22.909386094972188 40-41 22.745095687462676 25.98098341412155 28.846742010536484 22.427178887879293 42-43 22.544480412833014 26.066990188716037 28.522442069163766 22.86608732928718 44-45 22.467137518408464 26.20968732547867 28.753725942775475 22.56944921333739 46-47 23.01534904675125 26.57728192521061 27.837776617584563 22.569592410453577 48-49 22.52632678484382 26.73745173745174 27.68966504437446 23.046556433329982 50-51 22.80197460558182 26.800918439194994 27.68564155337702 22.711465401846162 52-53 23.239065002592056 26.396170719818013 27.686047771218114 22.678716506371817 54-55 22.573418100068128 26.7741377636113 27.806743915144544 22.845700221176028 56-57 22.69933689773369 26.920888108262858 27.460306713813726 22.919468280189722 58-59 22.563988981824654 26.44656107581959 27.625772781083402 23.36367716127235 60-61 22.17018980045032 26.812872849947 27.91865584450755 23.098281505095134 62-63 22.336294210041704 26.73087487716746 28.050812048808037 22.882018863982793 64-65 22.11896766505985 26.772812033333075 27.729435705387612 23.378784596219464 66-67 22.026914885591424 26.827335805463836 27.649580337470958 23.49616897147378 68-69 22.787100278530517 26.38948505222133 27.679577130359984 23.143837538888175 70-71 22.566213508097277 26.53118979271284 27.344360535743302 23.558236163446583 72-73 22.413639020759287 26.65289136299292 27.34783363941537 23.58563597683242 74-75 22.420419990560273 27.130651263917798 27.052812188082733 23.396116557439203 76-77 22.39649182534954 26.588776007613685 27.51636477588381 23.498367391152964 78-79 22.458623811329222 26.870284971487372 27.38997299618542 23.281118220997982 80-81 22.724043206885433 26.832028309907134 27.08746200653469 23.356466476672743 82-83 22.62828549227278 26.878550939215017 27.191903099340898 23.301260469171304 84-85 22.413885823853217 27.005294215052533 27.12287035905023 23.457949602044025 86-87 22.66972172706988 26.91702299903377 26.837054955300882 23.576200318595472 88-89 22.396784299084946 27.029335726796962 26.59336684311368 23.980513131004415 90-91 22.260172814578805 26.91305901225765 26.945769555672435 23.880998617491105 92-93 22.42149959484473 26.596039982025772 27.03264822212374 23.94981220100576 94-95 22.11546645414381 26.36440835338631 27.27197638520284 24.248148807267043 96-97 22.4284519673424 25.926278411316535 27.256758160482775 24.388511460858293 98-99 22.07672100958674 26.274692241626106 26.888255275028822 24.760331473758328 100-101 22.190773404281455 25.93862696120144 27.30958436041171 24.5610152741054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 554.0 1 380.5 2 291.5 3 389.0 4 366.0 5 384.5 6 671.5 7 1564.0 8 2460.5 9 2747.0 10 2447.0 11 2015.5 12 1991.5 13 2108.5 14 2343.0 15 2642.5 16 2935.5 17 3222.5 18 3491.5 19 3869.0 20 4325.0 21 4924.0 22 5719.0 23 6936.5 24 8582.5 25 10629.5 26 13790.0 27 17645.5 28 20433.0 29 23594.5 30 27948.0 31 34152.5 32 43989.0 33 47894.5 34 47293.0 35 50427.5 36 55533.5 37 59972.5 38 63326.5 39 66150.0 40 69392.5 41 74610.5 42 78056.5 43 80551.5 44 83794.5 45 88715.5 46 91198.5 47 92764.5 48 94498.0 49 93394.0 50 92575.0 51 91382.0 52 89930.0 53 91448.0 54 94338.0 55 90530.5 56 82965.5 57 75886.5 58 71300.0 59 64506.0 60 52806.5 61 44642.5 62 38249.5 63 28204.0 64 19231.5 65 14503.5 66 10847.5 67 8469.0 68 6687.5 69 5051.0 70 3839.5 71 2684.5 72 1973.0 73 1698.0 74 1302.0 75 913.5 76 677.5 77 465.0 78 333.0 79 226.5 80 132.0 81 78.5 82 49.5 83 23.5 84 11.0 85 8.5 86 5.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.737482687382487E-5 2 0.04619277164367345 3 1.5474965374764974E-4 4 0.0 5 0.09965877701348642 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011219349896704606 26-27 0.011857692218413662 28-29 0.0 30-31 0.0 32-33 4.8359266796140545E-4 34-35 0.014894654173211288 36-37 0.003462523502603663 38-39 0.012225222646064328 40-41 0.007021765538799607 42-43 0.005532300121478478 44-45 0.002766150060739239 46-47 5.8031120155368654E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.8687413436912436E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.934370671845622E-4 68-69 1.7409336046610594E-4 70-71 0.0 72-73 3.8687413436912436E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9343706718456218E-5 82-83 0.0011025912829520045 84-85 0.004332990304934193 86-87 0.0014507780038842163 88-89 9.67185335922811E-5 90-91 0.0010252164560781794 92-93 3.288430142137557E-4 94-95 0.0 96-97 1.9343706718456218E-5 98-99 0.0 100-101 7.930919754567049E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2584820.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.20937598466685 #Duplication Level Percentage of deduplicated Percentage of total 1 69.35690146220553 29.968684324119284 2 15.057110230614693 13.012166743944078 3 6.0283333384237405 7.8144156534255975 4 3.02509011751361 5.228490251005824 5 1.7297501911322288 3.737071318409091 6 1.0972943004993982 2.84480411976663 7 0.6898801260835216 2.086650282660458 8 0.5013391008585901 1.7330039763850904 9 0.3671181034151617 1.4276649745119177 >10 1.8991425138300326 14.871818250984884 >50 0.15415112522335023 4.564322414727013 >100 0.08482398526732253 6.900910323207941 >500 0.004128798286271215 1.2232226594923274 >1k 0.004667337193173892 3.6835135868915887 >5k 2.6926945345233995E-4 0.9032611204681671 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9656 0.37356566414682646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7931 0.30682987596815253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5703 0.22063431883071163 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4065 0.15726433562104905 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3720 0.14391717798531425 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3708 0.1434529290240713 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3350 0.12960283501365666 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3194 0.12356759851749831 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3041 0.11764842426165072 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3034 0.11737761236759234 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2649 0.10248295819438102 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8687413436912436E-5 2 0.0 0.0 0.0 0.0 3.8687413436912436E-5 3 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 4 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 5 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 6 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 7 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 8 0.0 0.0 0.0 3.8687413436912436E-5 3.8687413436912436E-5 9 0.0 0.0 0.0 7.737482687382487E-5 7.737482687382487E-5 10-11 0.0 0.0 0.0 7.737482687382487E-5 9.67185335922811E-5 12-13 0.0 0.0 0.0 7.737482687382487E-5 1.1606224031073731E-4 14-15 0.0 0.0 0.0 7.737482687382487E-5 1.3540594702919352E-4 16-17 0.0 0.0 0.0 1.1606224031073731E-4 1.5474965374764974E-4 18-19 0.0 0.0 0.0 1.3540594702919352E-4 1.5474965374764974E-4 20-21 0.0 0.0 0.0 2.127807739030184E-4 1.5474965374764974E-4 22-23 0.0 0.0 0.0 4.642489612429492E-4 1.934370671845622E-4 24-25 0.0 0.0 0.0 0.001257340936699654 2.3212448062147461E-4 26-27 0.0 0.0 0.0 0.002321244806214746 2.3212448062147461E-4 28-29 0.0 0.0 0.0 0.005687049775226128 2.3212448062147461E-4 30-31 0.0 0.0 0.0 0.016693618898027715 2.3212448062147461E-4 32-33 0.0 0.0 0.0 0.03953853653252451 2.514681873399308E-4 34-35 0.0 0.0 0.0 0.07337067958310443 2.7081189405838704E-4 36-37 0.0 0.0 0.0 0.12960283501365666 2.7081189405838704E-4 38-39 0.0 0.0 0.0 0.2139607400128442 2.7081189405838704E-4 40-41 0.0 0.0 0.0 0.3046440371089669 2.7081189405838704E-4 42-43 0.0 0.0 0.0 0.39977638675033467 2.9015560077684326E-4 44-45 0.0 0.0 0.0 0.520423085553346 3.094993074952995E-4 46-47 0.0 0.0 0.0 0.6673578817867396 3.094993074952995E-4 48-49 0.0 0.0 0.0 0.8180647008302319 3.288430142137557E-4 50-51 0.0 0.0 0.0 0.982795707244605 3.4818672093221194E-4 52-53 0.0 0.0 0.0 1.1453602185065113 3.4818672093221194E-4 54-55 0.0 0.0 0.0 1.3183703313963835 3.4818672093221194E-4 56-57 0.0 0.0 0.0 1.5210730340990861 3.4818672093221194E-4 58-59 0.0 0.0 0.0 1.737606487105485 3.4818672093221194E-4 60-61 0.0 0.0 0.0 1.9789966032451 3.4818672093221194E-4 62-63 0.0 0.0 0.0 2.217736631564287 3.4818672093221194E-4 64-65 0.0 0.0 0.0 2.4661678569494203 3.4818672093221194E-4 66-67 0.0 0.0 0.0 2.7537507447327085 3.4818672093221194E-4 68-69 0.0 0.0 0.0 3.0742953087642464 3.4818672093221194E-4 70-71 0.0 0.0 0.0 3.4125587081498905 4.0621784108758055E-4 72-73 0.0 0.0 0.0 3.7656200431751534 5.029363746798616E-4 74-75 0.0 0.0 0.0 4.120963935593194 5.029363746798616E-4 76-77 0.0 0.0 0.0 4.502770018802083 5.029363746798616E-4 78-79 0.0 0.0 0.0 4.933515680008666 5.029363746798616E-4 80-81 0.0 0.0 0.0 5.38505582593759 5.222800813983178E-4 82-83 0.0 0.0 0.0 5.863348318258138 5.609674948352303E-4 84-85 0.0 0.0 0.0 6.350674321616205 5.803112015536865E-4 86-87 0.0 0.0 0.0 6.851270107783134 5.996549082721427E-4 88-89 0.0 0.0 0.0 7.378772989995435 6.383423217090552E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5045 0.0 57.603718 1 GTATCAA 9645 0.0 44.086514 1 TGGTATC 2190 0.0 32.143124 2 TATCAAC 13235 0.0 31.948338 2 ATCAACG 13300 0.0 31.77681 3 TCAACGC 13335 0.0 31.764708 4 CAACGCA 13540 0.0 31.213558 5 AACGCAG 14105 0.0 30.00219 6 TAAGGTG 1930 0.0 28.080688 5 GTAAGGT 1930 0.0 27.834366 4 GTGGTAT 2715 0.0 27.329092 1 AGGTAAG 1720 0.0 26.823395 2 ACGCAGA 16090 0.0 26.15327 7 CGCAGAG 16275 0.0 25.739254 8 AAGGTAA 1685 0.0 25.686913 1 GGTAAGG 2040 0.0 24.702213 3 GTACATG 12885 0.0 23.846165 1 TACATGG 12590 0.0 23.800455 2 GCAGAGT 18205 0.0 22.48873 9 ACATGGG 12955 0.0 22.017805 3 >>END_MODULE