FastQCFastQC Report
Thu 26 May 2016
SRR938568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584253
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.36312234134970534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71800.2778365740506057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51290.19847127970829484No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37060.1434070116199923No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36980.14309744440656544No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35270.13648044521956637No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31910.1234786222556383No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31810.1230916632388547No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30970.11984120749787268No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29600.11453986896793773No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27580.10672329682890956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.059.494941
GTATCAA94750.046.1492161
TGGTATC18950.036.3664362
TATCAAC128350.033.622652
ATCAACG131300.032.9034273
TCAACGC133150.032.4819564
CAACGCA136050.031.6847845
AACGCAG141400.030.4776956
TAAGGTG16000.029.7044835
GTGGTAT25700.028.016771
AGGTAAG15050.027.7899742
ACGCAGA160400.026.5957437
GTAAGGT16350.026.4524334
CGCAGAG162400.026.2389568
GGTAAGG17150.024.6642463
TACATGG127000.024.0629712
AAGGTAA16900.023.7010081
GTACATG129850.023.5391241
GCAGAGT178500.023.5249659
ACATGGG133750.022.0668223