##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2584253 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.117522936028323 31.0 31.0 34.0 28.0 34.0 2 31.285049877082468 31.0 31.0 34.0 28.0 34.0 3 31.3335393245166 31.0 31.0 34.0 28.0 34.0 4 35.01616327813105 37.0 35.0 37.0 32.0 37.0 5 35.01415302603886 37.0 35.0 37.0 32.0 37.0 6 34.994981141552316 37.0 35.0 37.0 32.0 37.0 7 34.91658479258803 37.0 35.0 37.0 32.0 37.0 8 34.872851845388205 37.0 35.0 37.0 32.0 37.0 9 36.35725488177822 39.0 35.0 39.0 32.0 39.0 10-11 36.201125044645394 39.0 35.0 39.0 32.0 39.0 12-13 36.028740607053564 38.0 35.0 39.0 30.5 39.0 14-15 37.162991587897935 39.0 36.0 41.0 31.0 41.0 16-17 37.10080553258524 39.0 36.0 41.0 31.0 41.0 18-19 37.118202242582285 39.0 36.0 41.0 31.0 41.0 20-21 37.06158317316455 39.0 36.0 41.0 31.0 41.0 22-23 36.92125093789192 39.0 36.0 41.0 30.5 41.0 24-25 36.77699571210714 39.0 36.0 41.0 30.0 41.0 26-27 36.5698522938737 39.0 36.0 41.0 30.0 41.0 28-29 36.34212962120969 39.0 35.5 40.5 29.5 41.0 30-31 36.051515273465874 38.5 35.0 40.0 29.0 41.0 32-33 35.74126720564898 38.0 35.0 40.0 27.5 41.0 34-35 35.44464841484174 38.0 34.0 40.0 27.0 41.0 36-37 35.21273226731284 38.0 34.0 40.0 26.0 41.0 38-39 34.97009174411329 38.0 34.0 40.0 25.0 41.0 40-41 34.68912718685051 38.0 33.5 40.0 24.5 41.0 42-43 34.26208115072325 38.0 33.0 40.0 24.0 41.0 44-45 33.7798907459912 38.0 33.0 40.0 22.5 41.0 46-47 33.26663198224013 37.0 32.0 40.0 19.0 41.0 48-49 32.75221737190593 37.0 31.0 40.0 16.0 41.0 50-51 31.999010739273594 36.0 30.5 38.5 14.0 40.5 52-53 31.99983863809 36.0 31.0 39.0 12.5 40.0 54-55 31.99330386769407 36.0 31.0 39.0 8.5 40.0 56-57 31.58698567825983 36.0 30.0 39.0 7.5 40.0 58-59 31.1503862044467 35.5 29.5 39.0 4.0 40.0 60-61 30.614975391341325 35.0 28.5 38.5 2.0 40.0 62-63 29.920157198230978 34.5 28.0 38.0 2.0 40.0 64-65 29.28932306550481 34.0 26.5 38.0 2.0 40.0 66-67 28.60902821821238 34.0 26.0 37.0 2.0 39.5 68-69 27.79764926266894 33.5 25.5 36.5 2.0 39.0 70-71 27.00020315348381 32.5 23.0 36.0 2.0 39.0 72-73 26.14034055489149 32.0 21.0 35.0 2.0 38.0 74-75 25.24273436076112 31.0 17.5 35.0 2.0 37.5 76-77 24.34229543314838 30.5 10.5 34.5 2.0 36.5 78-79 23.384584055818067 30.0 4.5 34.0 2.0 36.0 80-81 22.45560167677081 29.0 2.0 34.0 2.0 35.5 82-83 21.47446767015459 28.5 2.0 34.0 2.0 35.0 84-85 20.51432367496526 26.5 2.0 33.0 2.0 35.0 86-87 19.551581056498726 26.0 2.0 33.0 2.0 35.0 88-89 18.452024821099172 24.5 2.0 32.0 2.0 34.0 90-91 17.30024459679451 21.0 2.0 31.5 2.0 34.0 92-93 16.14111060333489 12.5 2.0 31.0 2.0 34.0 94-95 14.86570103623755 2.0 2.0 31.0 2.0 34.0 96-97 13.41467456940168 2.0 2.0 30.0 2.0 34.0 98-99 11.666738125098433 2.0 2.0 29.0 2.0 34.0 100-101 8.208639982230842 2.0 2.0 11.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4853762125713459 1101 2 0.4024924373767611 1101 3 0.367972641770848 1101 4 0.13522617832082773 1101 5 0.2859074707887288 1101 6 0.39193696825245183 1101 7 0.2849204042018627 1101 8 0.3498667947966396 1101 9 0.404120628969423 1101 10-11 0.39847323022401326 1101 12-13 0.3252649833377319 1101 14-15 0.42984490045215296 1101 16-17 0.43583035969454187 1101 18-19 0.3553771888475197 1101 20-21 0.5479527609703041 1101 22-23 0.47419199932652845 1101 24-25 0.44062243554045466 1101 26-27 0.5176377090554425 1101 28-29 0.6792936475658209 1101 30-31 0.7925489174644866 1101 32-33 0.649682868149533 1101 34-35 0.6646446987441763 1101 36-37 0.7545300127128058 1101 38-39 0.8901165283655033 1101 40-41 0.7681565626618791 1101 42-43 1.0207267104114663 1101 44-45 1.1274699656255152 1101 46-47 1.136724707432272 1101 48-49 1.099712302805088 1101 50-51 1.1767085415601457 1101 52-53 1.1488048038622054 1101 54-55 1.2585181013592788 1101 56-57 1.4594823603704263 1101 58-59 1.3495151739532787 1101 60-61 1.298325661477822 1101 62-63 1.2416865536295845 1101 64-65 1.3127869647108739 1101 66-67 1.6681675364731454 1101 68-69 1.8033363580685773 1101 70-71 2.009431631657762 1101 72-73 2.2791606784889638 1101 74-75 2.1691630193994698 1101 76-77 2.3918237189690963 1101 78-79 2.3399883283085963 1101 80-81 2.583474358315332 1101 82-83 2.8114574580211524 1101 84-85 2.8579129919797808 1101 86-87 3.0178287323249045 1101 88-89 3.5295556858160886 1101 90-91 3.5604795788768335 1101 92-93 3.2965913156705575 1101 94-95 3.0007236867593985 1101 96-97 3.1040078323996294 1101 98-99 3.001436867882246 1101 100-101 2.141745377309819 1102 1 0.7474458707927774 1102 2 0.5923103617568124 1102 3 0.6211813620897999 1102 4 0.4780141920133616 1102 5 0.5071459543721133 1102 6 0.5603255690452968 1102 7 0.6049166457230086 1102 8 0.6421343725704176 1102 9 0.7249821306437312 1102 10-11 0.8063749460020091 1102 12-13 0.7324661154174876 1102 14-15 0.8836723117512406 1102 16-17 0.9194669633904837 1102 18-19 0.8931816539852022 1102 20-21 0.9056611256917932 1102 22-23 0.81759327688534 1102 24-25 0.9243943930761205 1102 26-27 1.0847771826416022 1102 28-29 0.926876718858658 1102 30-31 0.8723655620176842 1102 32-33 0.8551582590232343 1102 34-35 0.9901051529045048 1102 36-37 1.0006988753355373 1102 38-39 1.115224301126581 1102 40-41 1.176656313986662 1102 42-43 1.5157260704980402 1102 44-45 1.2536937798011465 1102 46-47 1.2726040051799359 1102 48-49 1.3979067749251115 1102 50-51 1.4783999380456407 1102 52-53 1.5550186872294773 1102 54-55 1.5129304259298522 1102 56-57 1.6249794434992317 1102 58-59 1.704848730851296 1102 60-61 1.8049073735621448 1102 62-63 1.8486352034122575 1102 64-65 2.015481000231741 1102 66-67 2.2748048157232645 1102 68-69 2.279083396241436 1102 70-71 2.2145047680921657 1102 72-73 2.2136178293999933 1102 74-75 2.2160978340918795 1102 76-77 2.264174598890559 1102 78-79 2.7243767087768163 1102 80-81 2.9153611633005028 1102 82-83 3.1779357147349785 1102 84-85 3.1120513688029234 1102 86-87 3.2400006794923577 1102 88-89 3.3251137956252315 1102 90-91 3.3049535619410015 1102 92-93 3.1698982586502176 1102 94-95 3.2189685095357827 1102 96-97 3.181513660471902 1102 98-99 3.0094008739670937 1102 100-101 2.4161613145561827 1103 1 0.5030611816968609 1103 2 0.43510668884905357 1103 3 0.5533417863332879 1103 4 0.4820871399539257 1103 5 0.4354939590341971 1103 6 0.44801265439623705 1103 7 0.5089294839993102 1103 8 0.41342220219699044 1103 9 0.49400109946801507 1103 10-11 0.6766071096150981 1103 12-13 0.5745260592619843 1103 14-15 0.5716333983171324 1103 16-17 0.6877741030474027 1103 18-19 0.83760173922294 1103 20-21 0.7945621233208939 1103 22-23 0.812687483238463 1103 24-25 0.8051453493299974 1103 26-27 0.8668813238307465 1103 28-29 0.9040210155357258 1103 30-31 0.9075464570298593 1103 32-33 1.0204719533440638 1103 34-35 1.157021319853115 1103 36-37 0.9870990239355635 1103 38-39 0.9534766883731223 1103 40-41 1.1897758461198222 1103 42-43 1.2907721093987163 1103 44-45 1.2096707053432851 1103 46-47 1.3431281869630354 1103 48-49 1.1406047262538195 1103 50-51 1.3409838245101255 1103 52-53 1.3472356815230455 1103 54-55 1.2544567968527147 1103 56-57 1.5150270688486067 1103 58-59 1.5585925903381792 1103 60-61 1.70042258672823 1103 62-63 1.8806542822515837 1103 64-65 2.0086137501133514 1103 66-67 2.1794807736114237 1103 68-69 2.211364518139689 1103 70-71 2.1973564534746757 1103 72-73 2.5481592022281916 1103 74-75 2.4828692276316033 1103 76-77 2.5272777882104904 1103 78-79 2.8487935095077006 1103 80-81 2.781933015076767 1103 82-83 3.0197943629531316 1103 84-85 3.138909706109022 1103 86-87 3.1839239432079616 1103 88-89 3.3365250653103473 1103 90-91 3.256238107060714 1103 92-93 3.3027495454275275 1103 94-95 3.2814941996409654 1103 96-97 3.1417556616613407 1103 98-99 2.933121710017586 1103 100-101 2.1584509347173153 1104 1 0.6064323035508252 1104 2 0.5148838966596578 1104 3 0.4625116256096149 1104 4 0.47590680038747735 1104 5 0.4965916794516687 1104 6 0.5310775089510855 1104 7 0.5453402360782391 1104 8 0.3941809621598438 1104 9 0.6892711967445493 1104 10-11 0.7214491507709084 1104 12-13 0.7747670159988544 1104 14-15 0.8678552784917173 1104 16-17 0.9047044763220313 1104 18-19 0.8439010043086768 1104 20-21 0.7541158572034519 1104 22-23 0.8555913414247058 1104 24-25 0.9235413091952864 1104 26-27 0.9970721197530494 1104 28-29 0.9104949572122223 1104 30-31 0.9131632259825722 1104 32-33 0.8981254695247642 1104 34-35 0.8985497324474068 1104 36-37 1.1068398269024868 1104 38-39 1.1937850160379284 1104 40-41 1.06465347403293 1104 42-43 1.2144510135265705 1104 44-45 1.2621209497844674 1104 46-47 1.2509437145454285 1104 48-49 1.2291534864742246 1104 50-51 1.150461506659326 1104 52-53 1.4028205602568846 1104 54-55 1.5574522049641928 1104 56-57 1.8506700317150582 1104 58-59 1.6850406410958954 1104 60-61 1.8344728566209838 1104 62-63 1.9486290033825995 1104 64-65 1.8683309497733944 1104 66-67 2.1387029552659875 1104 68-69 2.402996555008116 1104 70-71 2.4309870753566685 1104 72-73 2.6458182632165297 1104 74-75 2.6547612190204894 1104 76-77 2.8099260247041116 1104 78-79 2.9933227083738956 1104 80-81 3.362632539526185 1104 82-83 3.607183712983506 1104 84-85 3.6900822208307886 1104 86-87 3.552988612935888 1104 88-89 3.615362231277352 1104 90-91 3.500370534035145 1104 92-93 3.3905694512869964 1104 94-95 3.330319109462094 1104 96-97 3.45127439962676 1104 98-99 3.0198908293779017 1104 100-101 2.3140867166033683 1105 1 0.5211420863534677 1105 2 0.4317061240279507 1105 3 0.33585280947857754 1105 4 0.41510634004113456 1105 5 0.4426218422514694 1105 6 0.47549265952294206 1105 7 0.46872951316847633 1105 8 0.5435256643411606 1105 9 0.5809165192019918 1105 10-11 0.6437769198240062 1105 12-13 0.5165312036419891 1105 14-15 0.6655083892938407 1105 16-17 0.7099135729051937 1105 18-19 0.8534989341215748 1105 20-21 0.7148963459700113 1105 22-23 0.7347954268270627 1105 24-25 0.8119082496878391 1105 26-27 0.762523701029302 1105 28-29 0.7946367942861343 1105 30-31 0.9120713605727389 1105 32-33 0.9375192677920907 1105 34-35 0.9924128716968852 1105 36-37 1.0073591451201835 1105 38-39 1.010160625738969 1105 40-41 1.1092950263189678 1105 42-43 1.120260514655655 1105 44-45 1.1838042853868629 1105 46-47 1.358964318129999 1105 48-49 1.2399132107978446 1105 50-51 1.1442027449858472 1105 52-53 1.3857067163949282 1105 54-55 1.618315022925529 1105 56-57 1.7201274004637064 1105 58-59 1.6701442425945956 1105 60-61 1.8247038540842553 1105 62-63 1.764786182858387 1105 64-65 1.9455978205082722 1105 66-67 2.106642569665926 1105 68-69 2.3106469272180767 1105 70-71 2.210016902296786 1105 72-73 2.271969932650599 1105 74-75 2.4545348458779515 1105 76-77 2.590900009221425 1105 78-79 2.87741361649077 1105 80-81 3.058002661614257 1105 82-83 3.220652643533981 1105 84-85 3.0950205725068827 1105 86-87 3.2780191024524505 1105 88-89 3.4917116522086786 1105 90-91 3.365333583041089 1105 92-93 3.545021263565001 1105 94-95 3.6152636954711017 1105 96-97 3.711386583333292 1105 98-99 3.51911196633794 1105 100-101 2.6804069187420403 1106 1 0.2520792522750739 1106 2 0.2561372561729023 1106 3 0.1099363048062223 1106 4 0.2249305046080181 1106 5 0.29747740321303695 1106 6 -0.19989863764418914 1106 7 0.17991685707924177 1106 8 -0.23317284880771894 1106 9 0.14355140596963878 1106 10-11 0.18696056278780304 1106 12-13 0.3675998918339971 1106 14-15 0.4647712826275594 1106 16-17 -0.05526230437491364 1106 18-19 0.31502461087094247 1106 20-21 0.3766087021000928 1106 22-23 0.2816361471754121 1106 24-25 0.2781712836274082 1106 26-27 0.27211670287503154 1106 28-29 0.3631350010035277 1106 30-31 0.09327652850745949 1106 32-33 0.4959173570031581 1106 34-35 0.24743604376497785 1106 36-37 0.3872370384204231 1106 38-39 0.5297574272905479 1106 40-41 0.7166765407779394 1106 42-43 0.2268092741995531 1106 44-45 0.8844137587621432 1106 46-47 0.995300176975114 1106 48-49 0.8320787446202544 1106 50-51 0.7123297689848158 1106 52-53 0.9029635045331474 1106 54-55 0.6131301122772044 1106 56-57 1.203523494968696 1106 58-59 1.2606752333525435 1106 60-61 1.5861582793745583 1106 62-63 1.6075555686116658 1106 64-65 1.5924688659685984 1106 66-67 1.895443857325418 1106 68-69 1.9122402370575848 1106 70-71 1.6640149349307372 1106 72-73 2.0217604187674425 1106 74-75 2.2471959595432764 1106 76-77 2.502107969744653 1106 78-79 2.572140590050175 1106 80-81 2.3281999673382536 1106 82-83 2.5295975332922964 1106 84-85 2.6188294121514843 1106 86-87 2.372564892534605 1106 88-89 2.3437970825407106 1106 90-91 2.5136885224354977 1106 92-93 2.666151743008651 1106 94-95 2.7504765905902904 1106 96-97 2.562927793681885 1106 98-99 2.2805031329424867 1106 100-101 1.806605416268047 1107 1 0.25992011902204126 1107 2 0.18422951593701953 1107 3 0.18059563737119433 1107 4 0.08995624184555595 1107 5 0.10213707807459826 1107 6 0.10739271308416676 1107 7 0.1338492600013268 1107 8 0.25071592213086547 1107 9 0.3669909234614295 1107 10-11 0.30219942298347746 1107 12-13 0.23755480833962395 1107 14-15 0.3888320792687239 1107 16-17 0.38035316873098424 1107 18-19 0.3951899856207248 1107 20-21 0.48396526249593563 1107 22-23 0.38721514836261406 1107 24-25 0.5384659078377467 1107 26-27 0.47914873143730574 1107 28-29 0.43890386847841967 1107 30-31 0.5077134831076648 1107 32-33 0.5695671810887077 1107 34-35 0.5172887045793857 1107 36-37 0.5660937786303109 1107 38-39 0.6756857226535118 1107 40-41 0.49308347726675805 1107 42-43 0.5607761992496023 1107 44-45 0.48064649228206946 1107 46-47 0.527924237825907 1107 48-49 0.4145589542444199 1107 50-51 0.7101194645574864 1107 52-53 0.7340187421043183 1107 54-55 0.7639596940687596 1107 56-57 0.9350042743650455 1107 58-59 1.0158289093579924 1107 60-61 1.2590676162724002 1107 62-63 1.2396985727625562 1107 64-65 1.238664715947369 1107 66-67 1.4230206682477125 1107 68-69 1.3471703045636758 1107 70-71 1.6033466549927837 1107 72-73 1.8258064511599699 1107 74-75 1.9627992139606363 1107 76-77 1.9876221078938272 1107 78-79 2.1647886874877145 1107 80-81 2.4377933709795947 1107 82-83 2.411415030714238 1107 84-85 2.5010528451696743 1107 86-87 2.2177068264670297 1107 88-89 2.467782108147965 1107 90-91 2.2754192216737827 1107 92-93 2.4844531828207757 1107 94-95 2.4439738218874663 1107 96-97 2.1076578884850417 1107 98-99 2.0076196307717193 1107 100-101 1.274131651785435 1108 1 0.31023863319031264 1108 2 0.1703133442550815 1108 3 0.06082543075239144 1108 4 0.10442957965490507 1108 5 0.005263257768724827 1108 6 0.1458823515077583 1108 7 0.07598304010608103 1108 8 0.22918298312367824 1108 9 0.24006505251938393 1108 10-11 0.20432700052589325 1108 12-13 0.12785078402163208 1108 14-15 0.2794932246423514 1108 16-17 0.3616470819117197 1108 18-19 0.33461426632859315 1108 20-21 0.303964969113963 1108 22-23 0.3520614966916682 1108 24-25 0.37628499968292317 1108 26-27 0.3574847181899443 1108 28-29 0.3726995640195838 1108 30-31 0.5755171736971647 1108 32-33 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0.2431890597368671 2316 92-93 0.24440950723547772 2316 94-95 0.10720612373756744 2316 96-97 -0.12775972727176033 2316 98-99 -0.0978570821112772 2316 100-101 0.1652610194110391 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7899.0 3 2690.0 4 2928.0 5 3363.0 6 4237.0 7 5436.0 8 6930.0 9 9326.0 10 12150.0 11 15442.0 12 19334.0 13 23607.0 14 27359.0 15 32002.0 16 38222.0 17 43789.0 18 42879.0 19 41238.0 20 44360.0 21 49009.0 22 54485.0 23 61157.0 24 67894.0 25 75503.0 26 84593.0 27 94125.0 28 104518.0 29 116788.0 30 131278.0 31 147481.0 32 166196.0 33 189269.0 34 213367.0 35 231716.0 36 222827.0 37 152880.0 38 37486.0 39 490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.783590807010704 18.44044256440597 14.892826023365119 22.88314060521821 2 15.66507748563873 20.001153469058192 39.86284556171814 24.470923483584937 3 19.159428940607484 24.29661767193543 29.801044307386682 26.742909080070405 4 13.025251074484977 16.38118259547257 36.45436603644151 34.13920029360094 5 13.826672712214005 37.64340589409889 33.593715212127854 14.936206181559248 6 32.41793575788314 35.66658987914677 17.20866395522836 14.70681040774173 7 28.96451717546466 33.13123868201564 19.90289082320989 18.001353319309807 8 27.48697473607848 33.711461806430584 19.452596323467077 19.34896713402386 9 27.37283395301035 16.38076362531379 19.573729307271414 36.67267311440444 10-11 25.324947678367266 26.392109628748184 26.771256383064994 21.51168630981955 12-13 26.49891107046428 23.774719610079426 27.094304800792568 22.63206451866373 14-15 23.189443855893252 25.0672619183552 25.7120512533689 26.03124297238265 16-17 22.41078416118417 28.24382527544067 26.53405339210227 22.811337171272893 18-19 22.567441327578745 26.369674718281228 29.196026181697697 21.866857772442334 20-21 23.213776274314665 25.518596065834963 29.30284059303955 21.96478706681082 22-23 22.877934466753196 25.445044904158916 29.361041359979968 22.315979269107928 24-25 22.57104648504724 25.74377643417401 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23.15302860774259 50-51 22.888037310189517 26.801721699144608 27.512433440313877 22.797807550351997 52-53 23.25728633259237 26.381725190556093 27.633420226500142 22.72756825035139 54-55 22.575266011532666 26.742114079382706 27.808424096810764 22.874195812273864 56-57 22.68009918625092 26.9208628778138 27.40831899041322 22.990718945522058 58-59 22.654532951750664 26.30147752674526 27.60722628470218 23.436763236801898 60-61 22.193862644185913 26.667123309406026 28.027802577090387 23.111211469317674 62-63 22.43172907850631 26.549008369545636 28.11099589137025 22.9082666605778 64-65 22.21540048783678 26.63712273046186 27.82743905813369 23.320037723567673 66-67 22.12296147411982 26.66892787943776 27.741810778671127 23.466299867771294 68-69 22.8625633682907 26.20633876397972 27.811617197476878 23.1194806702527 70-71 22.622767276198385 26.333809805127746 27.474962010291716 23.568460908382153 72-73 22.56503533309081 26.419496280930964 27.518285745245706 23.497182640732515 74-75 22.546369208674356 26.86481590379733 27.33750096508791 23.25131392244041 76-77 22.652972772162283 26.40986099716731 27.464494682909464 23.47267154776094 78-79 22.70307954956414 26.66501033946774 27.36246727737927 23.269442833588844 80-81 22.973158890859803 26.589035341222626 27.035751831825927 23.402053936091637 82-83 22.856282588680493 26.652543373204416 27.12800479583082 23.363169242284272 84-85 22.76069549111867 26.73431127056829 27.112381385072897 23.392611853240144 86-87 22.998935448556935 26.60399576346449 26.75835765755342 23.638711130425158 88-89 22.854359628522463 26.612810216096637 26.632122527521013 23.900707627859884 90-91 22.70855492439197 26.573973868898605 26.834386906890728 23.8830842998187 92-93 22.96586733003712 26.114871035953897 27.02596695944544 23.893294674563542 94-95 22.7010314226077 25.932988687211 27.213471465290457 24.152508424890843 96-97 23.01585381783349 25.46425996971471 27.0960275954156 24.423858617036203 98-99 22.763694962888373 25.741390833685184 26.579309827044607 24.915604376381843 100-101 23.760913375082257 24.67514856757745 26.94053170697594 24.623406350364355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 495.0 1 358.0 2 285.0 3 394.5 4 366.0 5 339.5 6 562.5 7 1270.0 8 2092.0 9 2373.0 10 2110.0 11 1804.0 12 1820.0 13 1973.0 14 2151.5 15 2387.5 16 2668.5 17 3024.5 18 3431.0 19 3822.5 20 4288.5 21 4854.5 22 5745.0 23 7011.5 24 8647.0 25 10786.0 26 13677.5 27 17396.0 28 20597.0 29 23590.5 30 27544.0 31 34006.5 32 42538.5 33 46275.5 34 47044.5 35 50229.0 36 55097.0 37 60059.0 38 63677.0 39 66024.0 40 69125.5 41 73951.0 42 77549.5 43 80609.5 44 83378.0 45 87243.0 46 90318.0 47 92308.0 48 93877.0 49 93058.0 50 91780.0 51 90238.5 52 89002.5 53 89798.0 54 91152.5 55 88209.0 56 82333.5 57 75974.0 58 71298.0 59 64814.0 60 54464.5 61 46245.0 62 39590.5 63 31128.5 64 22678.5 65 17056.0 66 12978.5 67 9910.5 68 7756.5 69 6043.0 70 4673.0 71 3369.0 72 2521.5 73 1995.0 74 1487.5 75 1102.0 76 819.0 77 603.0 78 451.0 79 307.0 80 184.5 81 107.5 82 65.5 83 42.0 84 26.5 85 16.5 86 12.5 87 10.5 88 4.0 89 2.5 90 4.0 91 5.5 92 4.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3898225135077719 2 0.02871235904534115 3 0.03536805413401861 4 0.04469376643850273 5 0.06330649514579262 6 0.052935993495992845 7 0.03772850413639841 8 0.038850685285070775 9 0.03552283774073204 10-11 0.03685784634863537 12-13 0.029118666012963902 14-15 0.02693234756813671 16-17 0.022250143465055473 18-19 0.02679691191226246 20-21 0.03420717708366789 22-23 0.03335586724674403 24-25 0.041307875041646465 26-27 0.036025884462550686 28-29 0.03138237626114781 30-31 0.03409108937863282 32-33 0.029196057816320613 34-35 0.030801937735972444 36-37 0.032659341016533595 38-39 0.015130097556237721 40-41 0.012479428291270245 42-43 0.01267290779966203 44-45 0.01104767992917102 46-47 0.019850997560997317 48-49 0.017103588541833945 50-51 0.011376595093437058 52-53 0.00917092869777069 54-55 0.007797224188189005 56-57 0.00872592582846958 58-59 0.004005025823709985 60-61 0.0068104786953908925 62-63 0.011666814356024739 64-65 0.00742961312224461 66-67 0.00363741475776559 68-69 0.005920472956788673 70-71 0.012750299603018746 72-73 0.010563981158191555 74-75 0.011279855339241166 76-77 0.005146554923221526 78-79 0.008609838123434509 80-81 0.011125071732527737 82-83 0.013233998373998211 84-85 0.008068095499937507 86-87 0.0025539295107715847 88-89 0.015826623786448155 90-91 0.025732774616107634 92-93 0.010718764764904984 94-95 0.020895786906312965 96-97 0.018980339773234278 98-99 0.011299203290080344 100-101 0.1233818825014424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2584253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.11106303194735 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91505759140075 33.16079753543204 2 14.086232203252171 12.99062282013615 3 5.491781287215523 7.596956192773918 4 2.755150621478287 5.081716958779769 5 1.5220058674774197 3.50906542451225 6 0.9263972063300805 2.5630295984223763 7 0.6296443528963398 2.032349931087898 8 0.447907514667689 1.6522793313059752 9 0.32263053362836464 1.3389153184951497 >10 1.6884567579493102 14.05256143557995 >50 0.13307372831953615 4.214664978893624 >100 0.07336987255571209 6.264096145748057 >500 0.004315465349573639 1.4314844331086147 >1k 0.003723146576100812 3.2650471229534768 >5k 2.5385090291596445E-4 0.8464127727707577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9384 0.36312234134970534 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7180 0.2778365740506057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5129 0.19847127970829484 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3706 0.1434070116199923 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3698 0.14309744440656544 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3527 0.13648044521956637 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3191 0.1234786222556383 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3181 0.1230916632388547 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3097 0.11984120749787268 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2960 0.11453986896793773 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2758 0.10672329682890956 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1608770503507203E-4 7 0.0 0.0 0.0 0.0 1.1608770503507203E-4 8 0.0 0.0 0.0 3.8695901678357347E-5 1.5478360671342939E-4 9 0.0 0.0 0.0 7.739180335671469E-5 1.5478360671342939E-4 10-11 0.0 0.0 0.0 9.673975419589336E-5 1.5478360671342939E-4 12-13 0.0 0.0 0.0 1.1608770503507203E-4 1.5478360671342939E-4 14-15 0.0 0.0 0.0 1.1608770503507203E-4 1.5478360671342939E-4 16-17 0.0 0.0 0.0 1.1608770503507203E-4 1.5478360671342939E-4 18-19 0.0 0.0 0.0 1.1608770503507203E-4 2.3217541007014407E-4 20-21 0.0 0.0 0.0 1.934795083917867E-4 2.3217541007014407E-4 22-23 0.0 0.0 0.0 3.869590167835734E-4 2.902192625876801E-4 24-25 0.0 0.0 0.0 7.352221318887895E-4 3.0956721342685877E-4 26-27 0.0 0.0 0.0 0.0018960991822395098 3.0956721342685877E-4 28-29 0.0 0.0 0.0 0.00524329467741742 3.0956721342685877E-4 30-31 0.0 0.0 0.0 0.0163296705082668 3.2891516426603745E-4 32-33 0.0 0.0 0.0 0.03881198938339242 3.869590167835734E-4 34-35 0.0 0.0 0.0 0.07118111113733833 3.869590167835734E-4 36-37 0.0 0.0 0.0 0.1254714611920737 3.869590167835734E-4 38-39 0.0 0.0 0.0 0.2128274592309654 4.256549184619308E-4 40-41 0.0 0.0 0.0 0.30486566137293836 4.256549184619308E-4 42-43 0.0 0.0 0.0 0.4014506319621183 4.256549184619308E-4 44-45 0.0 0.0 0.0 0.5214272751158652 4.256549184619308E-4 46-47 0.0 0.0 0.0 0.66077121705963 4.4500286930110946E-4 48-49 0.0 0.0 0.0 0.8087636930285076 4.6435082014028813E-4 50-51 0.0 0.0 0.0 0.9735114944241141 4.6435082014028813E-4 52-53 0.0 0.0 0.0 1.1353377552430044 5.030467218186455E-4 54-55 0.0 0.0 0.0 1.306373640661344 5.804385251753602E-4 56-57 0.0 1.9347950839178673E-5 0.0 1.5067603675027175 5.804385251753602E-4 58-59 0.0 5.8043852517536016E-5 0.0 1.7227028468187906 5.804385251753602E-4 60-61 0.0 7.739180335671469E-5 0.0 1.9608567736982407 5.804385251753602E-4 62-63 0.0 7.739180335671469E-5 0.0 2.2004811448414685 5.804385251753602E-4 64-65 0.0 7.739180335671469E-5 0.0 2.4514047192747768 5.804385251753602E-4 66-67 0.0 7.739180335671469E-5 0.0 2.7385283097281885 5.804385251753602E-4 68-69 0.0 7.739180335671469E-5 0.0 3.055292960867222 6.191344268537175E-4 70-71 0.0 7.739180335671469E-5 0.0 3.3867427066931914 6.191344268537175E-4 72-73 0.0 7.739180335671469E-5 0.0 3.7354508246677085 6.965262302104321E-4 74-75 0.0 7.739180335671469E-5 0.0 4.082204799607469 6.965262302104321E-4 76-77 0.0 7.739180335671469E-5 0.0 4.455252639737672 6.965262302104321E-4 78-79 0.0 7.739180335671469E-5 0.0 4.858676762685388 6.965262302104321E-4 80-81 0.0 1.1608770503507203E-4 0.0 5.287272569674873 6.965262302104321E-4 82-83 0.0 1.1608770503507203E-4 0.0 5.735119587749342 6.965262302104321E-4 84-85 0.0 1.5478360671342939E-4 0.0 6.1926792771450785 7.158741810496109E-4 86-87 0.0 1.5478360671342939E-4 0.0 6.6558305243333376 7.352221318887895E-4 88-89 0.0 1.5478360671342939E-4 3.8695901678357347E-5 7.13680123424448 7.352221318887895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 59.49494 1 GTATCAA 9475 0.0 46.149216 1 TGGTATC 1895 0.0 36.366436 2 TATCAAC 12835 0.0 33.62265 2 ATCAACG 13130 0.0 32.903427 3 TCAACGC 13315 0.0 32.481956 4 CAACGCA 13605 0.0 31.684784 5 AACGCAG 14140 0.0 30.477695 6 TAAGGTG 1600 0.0 29.704483 5 GTGGTAT 2570 0.0 28.01677 1 AGGTAAG 1505 0.0 27.789974 2 ACGCAGA 16040 0.0 26.595743 7 GTAAGGT 1635 0.0 26.452433 4 CGCAGAG 16240 0.0 26.238956 8 GGTAAGG 1715 0.0 24.664246 3 TACATGG 12700 0.0 24.062971 2 AAGGTAA 1690 0.0 23.701008 1 GTACATG 12985 0.0 23.539124 1 GCAGAGT 17850 0.0 23.524965 9 ACATGGG 13375 0.0 22.066822 3 >>END_MODULE