FastQCFastQC Report
Thu 26 May 2016
SRR938568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584253
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95320.3688493347981022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78630.3042658748969238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.21696792071054963No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39050.15110749605398544No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37960.14688964277104447No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37720.14596094113076388No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33320.12893474439228667No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31780.12297557553381964No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31070.12022816651465627No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29520.11423030175451088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.059.2665181
GTATCAA96600.046.9301221
TATCAAC130650.034.61642
ATCAACG130900.034.5086563
TCAACGC131800.034.2716834
CAACGCA135950.033.225515
AACGCAG142450.031.8094566
TGGTATC19750.031.2701992
ACGCAGA163550.027.502357
CGCAGAG165300.027.038788
GTGGTAT25200.026.2116721
AGGTAAG15400.025.9127182
TAAGGTG18750.025.8386155
GTAAGGT18200.024.5316544
GCAGAGT179700.024.2641419
AAGGTAA16000.024.0572221
GGTAAGG18650.022.921913
TACATGG124500.022.513192
GTACATG127900.022.181161
GAGTACT100400.021.83274512-13
Produced by FastQC (version 0.11.5)