##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2584253 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79927932752714 31.0 31.0 34.0 30.0 34.0 2 32.049938995911006 31.0 31.0 34.0 30.0 34.0 3 32.098668357935544 33.0 31.0 34.0 30.0 34.0 4 35.76898740177529 37.0 35.0 37.0 35.0 37.0 5 35.660344788223135 37.0 35.0 37.0 33.0 37.0 6 35.69599338764432 37.0 35.0 37.0 33.0 37.0 7 35.61677784644151 37.0 35.0 37.0 33.0 37.0 8 35.58660084751764 37.0 35.0 37.0 33.0 37.0 9 37.12253444225469 39.0 37.0 39.0 33.0 39.0 10-11 37.056995193582054 39.0 37.0 39.0 33.0 39.0 12-13 36.93746239242056 39.0 37.0 39.0 33.0 39.0 14-15 37.93670588754274 40.0 37.0 41.0 33.0 41.0 16-17 38.022301609014285 40.0 37.0 41.0 33.0 41.0 18-19 38.17838462410607 40.0 37.5 41.0 33.0 41.0 20-21 38.13684012362567 40.0 37.0 41.0 33.0 41.0 22-23 38.01712641912383 40.0 37.0 41.0 33.0 41.0 24-25 37.88862642318689 40.0 37.0 41.0 32.5 41.0 26-27 37.6662946700652 40.0 37.0 41.0 32.0 41.0 28-29 37.52391406723723 39.5 36.5 41.0 32.0 41.0 30-31 37.24476918475088 39.0 36.0 41.0 31.0 41.0 32-33 37.10836767917073 39.0 36.0 41.0 31.0 41.0 34-35 36.96504599201394 39.0 36.0 41.0 31.0 41.0 36-37 36.82263114331298 39.0 35.0 41.0 30.0 41.0 38-39 36.59700114501173 39.0 35.0 40.0 30.0 41.0 40-41 36.35679768969989 39.0 35.0 40.0 30.0 41.0 42-43 36.06711126967832 38.0 35.0 40.0 29.5 41.0 44-45 35.73413032702294 38.0 35.0 40.0 28.5 41.0 46-47 35.2750051949248 38.0 34.0 40.0 27.0 41.0 48-49 34.82078592924145 38.0 34.0 40.0 26.0 41.0 50-51 34.39920298051313 38.0 33.0 40.0 24.0 41.0 52-53 33.79540548081012 37.0 33.0 40.0 23.0 41.0 54-55 33.35700161710173 37.0 32.0 40.0 22.0 41.0 56-57 32.96193464803949 36.0 31.5 39.0 20.5 40.0 58-59 32.43241102941546 36.0 31.0 39.0 18.5 40.0 60-61 31.947547511795477 35.0 31.0 39.0 17.5 40.0 62-63 31.455467788951005 35.0 30.0 38.0 13.5 40.0 64-65 30.811626996273198 34.5 29.0 38.0 8.0 40.0 66-67 30.173756207306326 34.0 28.0 37.5 7.0 39.5 68-69 29.46148693645707 34.0 27.5 36.5 3.5 39.0 70-71 28.702767879151153 33.0 26.0 36.0 2.0 39.0 72-73 28.14766510864068 33.0 26.0 35.5 2.0 38.5 74-75 27.46423860202542 32.0 25.0 35.0 2.0 37.5 76-77 26.326389869722508 30.5 24.0 34.0 2.0 36.0 78-79 26.844799638425492 32.0 25.0 35.0 2.0 36.0 80-81 26.493147923210305 32.0 24.0 35.0 2.0 36.0 82-83 25.83843822566908 31.5 22.0 34.5 2.0 36.0 84-85 25.089232168831767 31.0 19.5 34.0 2.0 35.0 86-87 24.21520648326615 30.5 16.0 34.0 2.0 35.0 88-89 23.242835744023516 30.0 7.0 34.0 2.0 35.0 90-91 22.403319257054164 29.0 2.0 34.0 2.0 35.0 92-93 21.34596341766847 29.0 2.0 33.5 2.0 35.0 94-95 20.179069928524797 27.0 2.0 33.0 2.0 34.5 96-97 18.80130796017263 25.5 2.0 32.5 2.0 34.0 98-99 17.018414412211186 19.5 2.0 32.0 2.0 34.0 100-101 12.729011052710398 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24223319239910523 1101 2 0.2170685679315909 1101 3 0.19468427235960206 1101 4 0.08576438913894435 1101 5 0.13925778259062582 1101 6 0.1697851855590713 1101 7 0.20427342520212477 1101 8 0.24590964827648776 1101 9 0.19034231976269922 1101 10-11 0.21756576366478697 1101 12-13 0.21222046476384548 1101 14-15 0.2976378117202074 1101 16-17 0.2395254725686513 1101 18-19 0.2773690860406006 1101 20-21 0.35233110244709565 1101 22-23 0.3392750208959896 1101 24-25 0.3161374445448786 1101 26-27 0.5211584364484452 1101 28-29 0.5197979091384894 1101 30-31 0.4970923498803046 1101 32-33 0.4434404855053131 1101 34-35 0.21185800233328678 1101 36-37 0.33289749681140535 1101 38-39 0.4141347510097262 1101 40-41 0.5403983275336302 1101 42-43 0.4690722224800936 1101 44-45 0.41700888388892565 1101 46-47 0.47952578148426994 1101 48-49 0.529981573842413 1101 50-51 0.5503458836505857 1101 52-53 0.5838677633230702 1101 54-55 0.6205146087643385 1101 56-57 0.7154031079929979 1101 58-59 0.7724081312344495 1101 60-61 0.7995516154757389 1101 62-63 0.8347685995789789 1101 64-65 0.8244497828217376 1101 66-67 1.104180756973026 1101 68-69 1.2670579256198558 1101 70-71 1.3247043995124805 1101 72-73 1.3628143936480406 1101 74-75 1.261410547243507 1101 76-77 1.1579870560531482 1101 78-79 1.4652474625817042 1101 80-81 1.5848465757690064 1101 82-83 1.7056324348159144 1101 84-85 1.9651776207089569 1101 86-87 1.9682796560078621 1101 88-89 1.7349021574982366 1101 90-91 2.1180761855448758 1101 92-93 2.474338457791422 1101 94-95 2.4339331279324483 1101 96-97 2.4829362555543426 1101 98-99 2.4497053197347967 1101 100-101 1.9651700252266817 1102 1 0.26298242571801467 1102 2 0.2965181127830405 1102 3 0.29804956896352763 1102 4 0.20613048441962434 1102 5 0.22432592932034368 1102 6 0.3035325995798175 1102 7 0.2873584883267455 1102 8 0.3145386621132005 1102 9 0.37894019654616073 1102 10-11 0.4299772490577496 1102 12-13 0.4515553760102833 1102 14-15 0.5475620751312533 1102 16-17 0.3669916098082098 1102 18-19 0.4058628918406413 1102 20-21 0.30588885908882446 1102 22-23 0.4190774091500913 1102 24-25 0.39089577597466985 1102 26-27 0.5484286305771207 1102 28-29 0.500868622002038 1102 30-31 0.42520172161492553 1102 32-33 0.5215448351345628 1102 34-35 0.5626403977479413 1102 36-37 0.5157722795243487 1102 38-39 0.5493750263507025 1102 40-41 0.5829626955340714 1102 42-43 0.5344056821760788 1102 44-45 0.5825121231468842 1102 46-47 0.42161709956012317 1102 48-49 0.4229194406788608 1102 50-51 0.7188749413902187 1102 52-53 0.6337727483296618 1102 54-55 0.5960374342332244 1102 56-57 0.6077193677881212 1102 58-59 0.7671072180212875 1102 60-61 0.6625280067204251 1102 62-63 0.8854952658244315 1102 64-65 1.0667450631767181 1102 66-67 1.1869999725201659 1102 68-69 1.2804117847838192 1102 70-71 1.4417823928087792 1102 72-73 1.3733247691557189 1102 74-75 1.2547699469237088 1102 76-77 1.432869563088147 1102 78-79 1.426066616567553 1102 80-81 1.7231315429876162 1102 82-83 1.670657926050719 1102 84-85 1.6975165771802665 1102 86-87 1.6495168273637155 1102 88-89 1.7316628995386054 1102 90-91 2.0652813029082395 1102 92-93 2.4162705265262225 1102 94-95 2.2269288299822776 1102 96-97 2.2700871989811375 1102 98-99 2.2864566007766705 1102 100-101 2.2586546535341814 1103 1 0.20642672542448537 1103 2 0.25827529206131317 1103 3 0.17895653929294753 1103 4 0.13986061594339816 1103 5 0.18735848579871828 1103 6 0.17778397915241584 1103 7 0.2190519067171195 1103 8 0.25610537188237714 1103 9 0.25878049935855785 1103 10-11 0.39438132631062217 1103 12-13 0.380763411253362 1103 14-15 0.40265074884420216 1103 16-17 0.3433327967269477 1103 18-19 0.40590139710302964 1103 20-21 0.3884867924005846 1103 22-23 0.47013363321085677 1103 24-25 0.3438220961949554 1103 26-27 0.44540879757676066 1103 28-29 0.409091050081706 1103 30-31 0.38933721868357907 1103 32-33 0.3897815811130414 1103 34-35 0.40687840013001164 1103 36-37 0.3962513073103153 1103 38-39 0.3289775213003736 1103 40-41 0.37718658729203014 1103 42-43 0.47295415336569135 1103 44-45 0.5512057504063819 1103 46-47 0.5144242687138245 1103 48-49 0.6545335366342968 1103 50-51 0.6065858633321639 1103 52-53 0.8021186324120748 1103 54-55 0.8494278903491761 1103 56-57 1.082018978590014 1103 58-59 1.2186222366680397 1103 60-61 1.089769403624377 1103 62-63 1.099265321645106 1103 64-65 1.282157292703161 1103 66-67 1.2842075836473121 1103 68-69 1.194737815138403 1103 70-71 1.435827151389553 1103 72-73 1.4295334437615352 1103 74-75 1.313296351329413 1103 76-77 1.4495842749683305 1103 78-79 1.6564502182684784 1103 80-81 1.8396134694306099 1103 82-83 2.017259178946592 1103 84-85 2.074188367252443 1103 86-87 2.1550393429770835 1103 88-89 2.09159295229799 1103 90-91 2.1171110602214505 1103 92-93 2.2700271413795328 1103 94-95 2.046834535936007 1103 96-97 2.1626384635959965 1103 98-99 2.179753576000966 1103 100-101 1.9963437319111268 1104 1 0.2155059692096799 1104 2 0.25617462804787294 1104 3 0.19273765876840798 1104 4 0.16834153559368303 1104 5 0.14343254988517629 1104 6 0.1159637672680418 1104 7 0.14754055273871813 1104 8 0.19808123227442564 1104 9 0.2067344501855075 1104 10-11 0.21902572385818786 1104 12-13 0.23460667499380605 1104 14-15 0.3257256325320057 1104 16-17 0.27523619587582715 1104 18-19 0.30401457177811153 1104 20-21 0.35363805505629387 1104 22-23 0.38356979293161686 1104 24-25 0.33294003243572945 1104 26-27 0.4037768364570695 1104 28-29 0.3357377897802607 1104 30-31 0.4056364534698247 1104 32-33 0.48705311155569575 1104 34-35 0.45079812464699387 1104 36-37 0.36433609135823275 1104 38-39 0.550462969210237 1104 40-41 0.6105913264361007 1104 42-43 0.6480702383072909 1104 44-45 0.5721320211293488 1104 46-47 0.5441663291098067 1104 48-49 0.545398011536463 1104 50-51 0.5460690599895202 1104 52-53 0.5834489166062014 1104 54-55 0.6689759127768724 1104 56-57 0.6085809554502859 1104 58-59 0.7846655164672143 1104 60-61 0.7188729021556135 1104 62-63 0.7342801759727138 1104 64-65 0.7982520058957121 1104 66-67 0.8731360722933346 1104 68-69 1.2020242377830357 1104 70-71 1.194747777316529 1104 72-73 1.2947433359407832 1104 74-75 1.2871077400007351 1104 76-77 1.4779245292113998 1104 78-79 1.5938543393028475 1104 80-81 1.8197082917013425 1104 82-83 1.9320694207820104 1104 84-85 2.130158125326126 1104 86-87 1.9077846287543494 1104 88-89 1.946375879138344 1104 90-91 1.9355103530312299 1104 92-93 2.264334547939672 1104 94-95 2.110645727660607 1104 96-97 2.477263045273588 1104 98-99 2.5654597133152492 1104 100-101 2.4771474866245935 1105 1 0.18383830086876074 1105 2 0.2242045288456893 1105 3 0.19696069074279166 1105 4 0.11345397715930972 1105 5 0.12476926572031033 1105 6 0.11136858961884144 1105 7 0.20116295873693701 1105 8 0.12805155919261324 1105 9 0.19108476026642762 1105 10-11 0.26608208014202006 1105 12-13 0.2428655946684799 1105 14-15 0.34293940572201365 1105 16-17 0.2550172570752949 1105 18-19 0.19542296387021452 1105 20-21 0.24770439584430903 1105 22-23 0.37293092666590866 1105 24-25 0.3044680311477208 1105 26-27 0.3540340312615484 1105 28-29 0.3699371915757226 1105 30-31 0.35887717086382764 1105 32-33 0.4179503026344378 1105 34-35 0.4890990574969152 1105 36-37 0.4574225818702402 1105 38-39 0.5255499234400318 1105 40-41 0.6931176655640954 1105 42-43 0.5825273849114083 1105 44-45 0.5678567030447894 1105 46-47 0.716514062599785 1105 48-49 0.6631112599367697 1105 50-51 0.653326872084385 1105 52-53 0.6006606897810514 1105 54-55 0.6770331166385546 1105 56-57 0.8054130171787506 1105 58-59 1.0499368820932702 1105 60-61 1.036990525046182 1105 62-63 1.043286158446577 1105 64-65 1.1679272334367212 1105 66-67 1.4135154494514737 1105 68-69 1.5662781385625664 1105 70-71 1.4642322462703632 1105 72-73 1.5622296469085661 1105 74-75 1.5651639881409025 1105 76-77 1.6459343727144997 1105 78-79 1.7354151254059396 1105 80-81 1.9271185335217424 1105 82-83 2.1721878102498167 1105 84-85 2.217985647448792 1105 86-87 2.2817896033634426 1105 88-89 2.2726588495665467 1105 90-91 2.31538087205087 1105 92-93 2.358505689521177 1105 94-95 2.2841206839709898 1105 96-97 2.3974143692764684 1105 98-99 2.409142172625298 1105 100-101 2.28564439381546 1106 1 0.09169571314075142 1106 2 0.031103449974736463 1106 3 0.006941084037450196 1106 4 0.09500043511927458 1106 5 -0.00744459578515233 1106 6 -0.1042312394222975 1106 7 0.05031477623499825 1106 8 0.031432878923631336 1106 9 -0.013053599242660141 1106 10-11 0.0769360291578991 1106 12-13 0.09771153082450468 1106 14-15 0.12028243211796763 1106 16-17 0.06158872558069817 1106 18-19 0.06074285055975537 1106 20-21 0.020615617753826143 1106 22-23 0.21656877675776087 1106 24-25 0.07830693087342411 1106 26-27 -0.044819096437585415 1106 28-29 0.16538098996478112 1106 30-31 0.30695455318453924 1106 32-33 0.11792795634676168 1106 34-35 0.0829011569878304 1106 36-37 0.12984637014906042 1106 38-39 0.3023037443483503 1106 40-41 0.212839589587837 1106 42-43 0.2442824947067237 1106 44-45 0.1653274575849082 1106 46-47 -0.32856406292329865 1106 48-49 -0.02904540232573538 1106 50-51 0.1692012452166054 1106 52-53 -0.03953422787525085 1106 54-55 -0.13997931303062217 1106 56-57 0.17986740229595455 1106 58-59 0.4398739526037545 1106 60-61 0.6290303086917142 1106 62-63 0.5811024379263756 1106 64-65 0.729651878630122 1106 66-67 0.7536020950198257 1106 68-69 0.7350265065303248 1106 70-71 0.5622004916655214 1106 72-73 0.7894934151231574 1106 74-75 0.7889211886113721 1106 76-77 0.885006661420725 1106 78-79 0.9269729573752485 1106 80-81 1.0270711627396416 1106 82-83 1.2110838813204978 1106 84-85 1.4149351838958353 1106 86-87 1.3552310544158672 1106 88-89 1.2673763657083228 1106 90-91 1.3955799130296676 1106 92-93 1.3485039821643632 1106 94-95 1.1735998787681297 1106 96-97 1.7551637884403775 1106 98-99 1.8334910615042688 1106 100-101 1.5994818233172463 1107 1 0.08380556001718631 1107 2 0.025456097790531373 1107 3 0.04426116168245642 1107 4 0.014188650584145535 1107 5 0.10152595804206754 1107 6 -0.03539821083403183 1107 7 0.046031194339455794 1107 8 0.07895848617511803 1107 9 0.1877981343379318 1107 10-11 0.16241999971172305 1107 12-13 0.12462405887982442 1107 14-15 0.219373233871913 1107 16-17 0.11834523788170515 1107 18-19 0.10047119055126075 1107 20-21 0.1771307351312288 1107 22-23 0.17650193143249027 1107 24-25 0.25465548196227417 1107 26-27 0.22434978360261937 1107 28-29 0.14920991316036236 1107 30-31 0.19976047534889574 1107 32-33 0.3204445945609251 1107 34-35 0.3556535898933646 1107 36-37 0.36057593850873104 1107 38-39 0.3580593640784997 1107 40-41 0.384459085761911 1107 42-43 0.37554863464104216 1107 44-45 0.3443239327965699 1107 46-47 0.4976727543249808 1107 48-49 0.535606679184184 1107 50-51 0.4111968126923742 1107 52-53 0.40605361236323034 1107 54-55 0.3553035474423538 1107 56-57 0.3913750129790756 1107 58-59 0.565909778925068 1107 60-61 0.750092273040881 1107 62-63 0.7937087015207567 1107 64-65 0.8687334297103035 1107 66-67 0.8618073559884785 1107 68-69 1.0272356844118988 1107 70-71 0.8327047075463518 1107 72-73 1.1562747764237145 1107 74-75 1.191505311248875 1107 76-77 1.361370052583407 1107 78-79 1.405927403764668 1107 80-81 1.7380168545687198 1107 82-83 1.8930681400100617 1107 84-85 1.9571587144035618 1107 86-87 2.207964444365345 1107 88-89 1.9706853772077544 1107 90-91 2.035215800444419 1107 92-93 2.127226565940372 1107 94-95 1.8927655633179867 1107 96-97 1.8992094525390968 1107 98-99 2.0932568049811344 1107 100-101 2.095571951310175 1108 1 -0.02608702063819024 1108 2 0.0054444611708532875 1108 3 -0.010392655372854165 1108 4 0.01921833408989926 1108 5 -0.09499634911379928 1108 6 0.018326390205423593 1108 7 0.01728651091418243 1108 8 -0.07290835787080852 1108 9 0.024421370086713523 1108 10-11 0.04826617564640401 1108 12-13 0.06137596729063688 1108 14-15 0.12103905933715708 1108 16-17 -0.029290804379833446 1108 18-19 0.0019920126231411928 1108 20-21 0.03838014252559674 1108 22-23 0.034318704781149734 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-0.3102235365160766 2316 58-59 -0.47523472048388626 2316 60-61 -0.4116232285053023 2316 62-63 -0.3182479158710301 2316 64-65 -0.29607302029283034 2316 66-67 -0.06814637134000279 2316 68-69 0.11633244304439216 2316 70-71 -0.05922129213062277 2316 72-73 0.07155033853995363 2316 74-75 0.22683151922933575 2316 76-77 0.31751504565337285 2316 78-79 0.17365305380086227 2316 80-81 -0.3668453979099162 2316 82-83 0.18964747160378082 2316 84-85 0.4072198720279161 2316 86-87 0.4901086851388463 2316 88-89 0.4653344539811677 2316 90-91 0.5556786532577753 2316 92-93 0.6392264859887504 2316 94-95 0.8993956454105927 2316 96-97 0.8835373250157268 2316 98-99 0.6306663161118529 2316 100-101 0.13011702121955082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 45.0 5 108.0 6 230.0 7 625.0 8 1354.0 9 2494.0 10 4108.0 11 6312.0 12 9077.0 13 12329.0 14 16470.0 15 22210.0 16 28083.0 17 35441.0 18 34245.0 19 30071.0 20 30664.0 21 33255.0 22 36550.0 23 40820.0 24 46613.0 25 53148.0 26 61196.0 27 72554.0 28 85199.0 29 99842.0 30 117286.0 31 137661.0 32 162872.0 33 195531.0 34 236654.0 35 287461.0 36 325129.0 37 273293.0 38 83785.0 39 1519.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.47517697280798 19.25848112838381 15.48322895011552 22.78311294869269 2 15.977629546151153 20.658806847207213 38.997633075639406 24.36593053100222 3 19.199755437832064 24.461595613359595 30.310810653932922 26.027838294875416 4 13.442298412732809 17.11411382709046 36.09286706835592 33.350720691820804 5 13.751647968089689 37.797683886538536 33.70936866231233 14.74129948305945 6 32.32311232685035 36.19065161189713 16.871567915370512 14.614668145882002 7 28.836611585630358 33.2019736457692 19.91569710860353 18.045717659996914 8 27.364960820354067 34.12669052916707 19.31829350875496 19.190055141723906 9 26.97594308903135 16.427764064348338 19.516942459447566 37.079350387172745 10-11 25.266798568096853 26.60163304444263 26.73964197777849 21.391926409682025 12-13 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25.58875576043999 28.76984979225848 22.601472552076622 38-39 22.322907507399655 26.104955911674736 28.677323151324508 22.8948134296011 40-41 22.772603121435267 25.960352682903704 28.849059031718433 22.417985163942593 42-43 22.523726350320487 26.05313765975343 28.511885861980158 22.911250127945927 44-45 22.45349244194742 26.209271967032354 28.764345904902832 22.572889686117396 46-47 23.024698452278823 26.53561902462261 27.83293882831041 22.60674369478816 48-49 22.548392127241414 26.764813661820263 27.625990953672108 23.06080325726622 50-51 22.84008183409287 26.795306032342808 27.62922206146225 22.735390072102074 52-53 23.315809249326595 26.37472027700074 27.618019597926363 22.6914508757463 54-55 22.548837130110712 26.81411224055849 27.772048634557066 22.86500199477373 56-57 22.734209847100885 26.987856839094317 27.35562268864542 22.92231062515938 58-59 22.561935692828836 26.422935370491974 27.60795866348999 23.407170273189195 60-61 22.160775280129304 26.775184163470062 27.938557099479038 23.125483456921593 62-63 22.336744893011637 26.72217077817071 28.03837317785836 22.90271115095929 64-65 22.126557317165663 26.781772830203863 27.70571523210585 23.385954620524625 66-67 22.017872555641567 26.86320144614967 27.62504974863998 23.493876249568782 68-69 22.826980756721447 26.370612953119053 27.613479775576995 23.1889265145825 70-71 22.52744701368133 26.532474387172766 27.311752104670905 23.628326494474997 72-73 22.44921295240218 26.603131705895706 27.299795124033256 23.647860217668864 74-75 22.373989698377056 27.07840524902167 27.033131044057995 23.51447400854328 76-77 22.393956783643088 26.543840715286006 27.541653235964127 23.520549265106784 78-79 22.488220000131566 26.804593048745613 27.38650201818475 23.320684932938068 80-81 22.692986359678823 26.839257037825288 27.065260713940216 23.402495888555674 82-83 22.612177645384868 26.82105712849529 27.188962115170224 23.377803110949618 84-85 22.407930657442044 26.968921656817646 27.104152570035545 23.51899511570476 86-87 22.684848015788557 26.867054931021805 26.74800224445175 23.70009480873788 88-89 22.43468422015956 26.99029468090005 26.493207127939872 24.081813971000518 90-91 22.258085237456932 26.868090747832223 26.91659675507001 23.957227259640835 92-93 22.402563229421414 26.543993591926444 26.998150326595056 24.055292852057082 94-95 22.07822184558204 26.305614486200557 27.20173435064879 24.41442931756862 96-97 22.36153394453522 25.925040219560593 27.157853189655423 24.55557264624877 98-99 22.04712541689997 26.232261314971872 26.731748013836103 24.988865254292055 100-101 22.228404969793967 25.820217997316053 27.1344280666127 24.816948966277284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 534.0 1 385.5 2 311.0 3 376.5 4 337.5 5 355.5 6 618.0 7 1440.0 8 2313.0 9 2501.5 10 2202.5 11 1920.0 12 1912.0 13 2094.5 14 2333.0 15 2602.0 16 2830.0 17 3078.0 18 3453.5 19 3894.5 20 4351.5 21 4942.5 22 5942.5 23 7139.0 24 8552.5 25 10705.5 26 13763.0 27 17537.0 28 20666.5 29 23762.0 30 27868.5 31 34335.0 32 43725.5 33 47512.5 34 47648.0 35 50607.0 36 55247.0 37 60153.0 38 63706.0 39 66120.5 40 69236.5 41 74318.0 42 77794.0 43 80398.0 44 83507.0 45 88130.5 46 91238.0 47 92821.0 48 93920.5 49 93263.0 50 92366.5 51 91155.5 52 89723.5 53 90961.0 54 94225.0 55 90681.0 56 83336.5 57 76017.5 58 70625.5 59 64273.5 60 53253.5 61 44873.5 62 38536.5 63 28855.0 64 19833.0 65 14841.5 66 11141.0 67 8622.5 68 6780.0 69 5199.5 70 3986.5 71 2774.5 72 2063.5 73 1684.0 74 1285.5 75 991.5 76 706.0 77 481.0 78 331.0 79 218.5 80 120.5 81 68.5 82 47.5 83 20.5 84 11.5 85 10.0 86 6.0 87 3.0 88 1.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02569407871442928 2 0.011957033618612419 3 0.0016639237721693657 4 0.0 5 3.869590167835734E-4 6 0.0 7 0.0 8 1.1608770503507203E-4 9 2.3217541007014407E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018380553297219738 26-27 0.009886802878820301 28-29 2.708713117485014E-4 30-31 0.0 32-33 3.676110659443948E-4 34-35 0.010718764764904984 36-37 0.001238268853707435 38-39 0.013504869685746712 40-41 0.013369434029872462 42-43 0.005939820907627852 44-45 8.706577877630403E-4 46-47 7.739180335671469E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.8695901678357347E-5 66-67 4.450028693011095E-4 68-69 0.001006093443637291 70-71 3.8695901678357347E-5 72-73 2.5152336090932274E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1608770503507203E-4 82-83 0.001006093443637291 84-85 0.005978516809306209 86-87 0.003985677872870806 88-89 0.0 90-91 0.0014317483620992217 92-93 5.030467218186455E-4 94-95 1.9347950839178673E-5 96-97 1.9347950839178673E-5 98-99 0.0 100-101 3.8695901678357347E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2584253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.25410657212183 #Duplication Level Percentage of deduplicated Percentage of total 1 69.40319257966279 30.019730882862287 2 15.083195856643025 13.048203220628473 3 5.964195711379369 7.739278707509856 4 3.0285553288718043 5.239898198183539 5 1.7143358439190914 3.7076032646642387 6 1.0690088033597838 2.7743412424236302 7 0.7058234144652903 2.137083283326641 8 0.5125559893197197 1.7736121108971592 9 0.36971626669895613 1.4392572121119298 >10 1.904235164963487 14.889413583806013 >50 0.15057218208588766 4.488299823303105 >100 0.08564535244028057 6.962913674796424 >500 0.004304402971483202 1.3273303850492824 >1k 0.004394078033387793 3.5608294566998198 >5k 2.6902518571762E-4 0.8922049537374922 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9532 0.3688493347981022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7863 0.3042658748969238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5607 0.21696792071054963 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3905 0.15110749605398544 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3796 0.14688964277104447 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3772 0.14596094113076388 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3332 0.12893474439228667 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3178 0.12297557553381964 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3107 0.12022816651465627 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2952 0.11423030175451088 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8695901678357347E-5 2 0.0 0.0 0.0 0.0 3.8695901678357347E-5 3 0.0 0.0 0.0 0.0 3.8695901678357347E-5 4 0.0 0.0 0.0 0.0 3.8695901678357347E-5 5 0.0 0.0 0.0 0.0 3.8695901678357347E-5 6 0.0 0.0 0.0 0.0 3.8695901678357347E-5 7 0.0 0.0 0.0 0.0 3.8695901678357347E-5 8 0.0 0.0 0.0 0.0 7.739180335671469E-5 9 0.0 0.0 0.0 0.0 7.739180335671469E-5 10-11 0.0 0.0 0.0 1.9347950839178673E-5 7.739180335671469E-5 12-13 0.0 0.0 0.0 3.8695901678357347E-5 7.739180335671469E-5 14-15 0.0 0.0 0.0 3.8695901678357347E-5 1.354356558742507E-4 16-17 0.0 0.0 0.0 7.739180335671469E-5 2.3217541007014407E-4 18-19 0.0 0.0 0.0 7.739180335671469E-5 2.3217541007014407E-4 20-21 0.0 0.0 0.0 1.5478360671342939E-4 2.3217541007014407E-4 22-23 0.0 0.0 0.0 3.4826311510521607E-4 2.3217541007014407E-4 24-25 0.0 0.0 0.0 6.965262302104321E-4 2.3217541007014407E-4 26-27 0.0 0.0 0.0 0.001876751231400331 2.3217541007014407E-4 28-29 0.0 0.0 0.0 0.005320686480774135 2.3217541007014407E-4 30-31 0.0 0.0 0.0 0.01648445411498023 2.708713117485014E-4 32-33 0.0 0.0 0.0 0.03896677299010584 3.2891516426603745E-4 34-35 0.0 0.0 0.0 0.07156807015412191 3.869590167835734E-4 36-37 0.0 0.0 0.0 0.12632277102899755 3.869590167835734E-4 38-39 0.0 0.0 0.0 0.21338854980530159 3.869590167835734E-4 40-41 0.0 0.0 0.0 0.30533001219307865 3.869590167835734E-4 42-43 0.0 0.0 0.0 0.4022052020448462 3.869590167835734E-4 44-45 0.0 0.0 0.0 0.523129894789713 3.869590167835734E-4 46-47 0.0 0.0 0.0 0.6637121055871851 4.256549184619308E-4 48-49 0.0 0.0 0.0 0.8120528446711681 4.256549184619308E-4 50-51 0.0 0.0 0.0 0.9765297747550259 4.256549184619308E-4 52-53 0.0 0.0 0.0 1.137775597048741 5.030467218186455E-4 54-55 0.0 0.0 0.0 1.3084051754994577 5.417426234970028E-4 56-57 0.0 0.0 0.0 1.5098947355386643 5.417426234970028E-4 58-59 0.0 0.0 0.0 1.7263596095273954 5.417426234970028E-4 60-61 0.0 0.0 0.0 1.9646102761610416 5.417426234970028E-4 62-63 0.0 0.0 0.0 2.204621606321053 5.417426234970028E-4 64-65 0.0 0.0 0.0 2.4551582217375776 5.417426234970028E-4 66-67 0.0 0.0 0.0 2.7421270285842754 5.417426234970028E-4 68-69 0.0 0.0 0.0 3.0615616969391155 5.417426234970028E-4 70-71 0.0 0.0 0.0 3.3987190882626432 5.417426234970028E-4 72-73 0.0 0.0 0.0 3.7539087697682847 6.771782793712534E-4 74-75 0.0 0.0 0.0 4.109117799224766 6.965262302104321E-4 76-77 0.0 0.0 0.0 4.4930391877265885 6.965262302104321E-4 78-79 0.0 0.0 0.0 4.912173846755716 6.965262302104321E-4 80-81 0.0 0.0 0.0 5.359363034501653 7.545700827279681E-4 82-83 0.0 0.0 0.0 5.830969336206633 8.126139352455042E-4 84-85 0.0 0.0 0.0 6.315287241612953 8.126139352455042E-4 86-87 0.0 0.0 0.0 6.8084084646511 8.126139352455042E-4 88-89 0.0 0.0 0.0 7.327339854108711 8.126139352455042E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4875 0.0 59.266518 1 GTATCAA 9660 0.0 46.930122 1 TATCAAC 13065 0.0 34.6164 2 ATCAACG 13090 0.0 34.508656 3 TCAACGC 13180 0.0 34.271683 4 CAACGCA 13595 0.0 33.22551 5 AACGCAG 14245 0.0 31.809456 6 TGGTATC 1975 0.0 31.270199 2 ACGCAGA 16355 0.0 27.50235 7 CGCAGAG 16530 0.0 27.03878 8 GTGGTAT 2520 0.0 26.211672 1 AGGTAAG 1540 0.0 25.912718 2 TAAGGTG 1875 0.0 25.838615 5 GTAAGGT 1820 0.0 24.531654 4 GCAGAGT 17970 0.0 24.264141 9 AAGGTAA 1600 0.0 24.057222 1 GGTAAGG 1865 0.0 22.92191 3 TACATGG 12450 0.0 22.51319 2 GTACATG 12790 0.0 22.18116 1 GAGTACT 10040 0.0 21.832745 12-13 >>END_MODULE