FastQCFastQC Report
Thu 26 May 2016
SRR938567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906565
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.22743765942714378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68900.17636977754113908No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA66760.17089181928369296No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA64990.1663609846501978No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63430.16236770666813427No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57170.14634339886831527No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52140.13346763716973864No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49640.12706815322412401No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG48870.1250971121688747No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48670.12458515345322553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.11905599932421448No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA45310.11598424703031947No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG40790.10441398005664823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.045.3028141
GTATCAA109200.040.896951
ATCAACG140850.031.301913
TCAACGC143250.030.9766854
TATCAAC142800.030.8411622
CAACGCA145500.030.268855
AACGCAG150700.029.328836
ACGCAGA171100.025.607827
CGCAGAG171550.025.3744378
TAAGGTG27450.023.910285
GTACATG122900.022.9483131
TACATGG120300.022.8117262
GTGTAAG15100.022.6779351
GCAGAGT189350.022.1095549
GTAAGGT27600.021.7124794
ACATGGG120400.021.2916933
GGTAAGG28900.021.2295043
AGGTAAG27900.020.9676072
AGAGTAC164250.020.7580210-11
GTGTAGC25900.020.5667741
Produced by FastQC (version 0.11.5)