##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3906565 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.184858820984676 31.0 31.0 34.0 28.0 34.0 2 31.22949010191818 31.0 31.0 34.0 28.0 34.0 3 31.280137921677994 31.0 31.0 34.0 27.0 34.0 4 34.93702549426414 37.0 35.0 37.0 32.0 37.0 5 34.92175632556991 37.0 35.0 37.0 32.0 37.0 6 34.934508961197366 37.0 35.0 37.0 32.0 37.0 7 34.845317561591834 37.0 35.0 37.0 32.0 37.0 8 34.807723153204925 37.0 35.0 37.0 32.0 37.0 9 36.31624636989273 39.0 35.0 39.0 32.0 39.0 10-11 36.15475974417423 39.0 35.0 39.0 31.0 39.0 12-13 36.030989628996316 38.5 35.0 39.0 30.0 39.0 14-15 37.02749474282394 39.0 36.0 41.0 31.0 41.0 16-17 36.86776810318016 39.0 36.0 41.0 31.0 41.0 18-19 37.00358409497858 39.0 36.0 41.0 31.0 41.0 20-21 36.97118094796835 39.0 36.0 41.0 31.0 41.0 22-23 36.89527090935387 39.0 36.0 41.0 30.0 41.0 24-25 36.765611989049205 39.0 36.0 41.0 30.0 41.0 26-27 36.569798403456744 39.0 36.0 41.0 30.0 41.0 28-29 36.37220332952351 39.0 35.5 41.0 29.5 41.0 30-31 36.11673836221847 39.0 35.0 40.0 29.0 41.0 32-33 35.84759564988679 38.0 35.0 40.0 27.5 41.0 34-35 35.60987888336685 38.0 35.0 40.0 27.0 41.0 36-37 35.34098575090904 38.0 34.0 40.0 26.5 41.0 38-39 35.204375455163294 38.0 34.0 40.0 25.5 41.0 40-41 35.106920657918145 38.0 34.0 40.0 25.0 41.0 42-43 34.82509378443723 38.0 33.5 40.0 24.5 41.0 44-45 34.50545325624941 38.0 33.0 40.0 24.0 41.0 46-47 34.1566790773992 38.0 33.0 40.0 23.0 41.0 48-49 33.80612379417724 38.0 33.0 40.0 21.5 41.0 50-51 33.099713687088276 37.5 32.0 39.5 20.0 40.5 52-53 33.11178592958264 37.0 32.0 39.5 20.0 40.0 54-55 33.22343171046687 37.0 32.0 40.0 19.0 41.0 56-57 32.917037218118736 37.0 32.0 40.0 17.5 41.0 58-59 32.55794847903465 36.5 31.0 39.5 14.0 41.0 60-61 32.09806172430255 36.0 31.0 39.0 11.0 41.0 62-63 31.54675091800597 35.5 30.0 39.0 8.0 40.0 64-65 31.042386341965383 35.0 29.5 38.5 6.5 40.0 66-67 30.52221363269266 35.0 29.0 38.0 2.0 40.0 68-69 29.966432018921992 34.0 28.5 37.5 2.0 40.0 70-71 29.345240127836092 34.0 28.0 37.0 2.0 39.0 72-73 28.729130834889474 34.0 26.5 36.0 2.0 39.0 74-75 28.076716117612275 33.5 26.0 36.0 2.0 38.5 76-77 27.360463476225277 33.0 25.5 35.0 2.0 37.5 78-79 26.607651478984735 32.0 23.5 35.0 2.0 37.0 80-81 25.93872737302464 32.0 21.5 35.0 2.0 36.5 82-83 25.228529283398586 31.0 19.0 34.5 2.0 36.0 84-85 24.5531818874126 31.0 16.5 34.0 2.0 35.5 86-87 23.825361538845506 30.5 8.5 34.0 2.0 35.0 88-89 23.023463963865954 30.0 3.5 34.0 2.0 35.0 90-91 22.143988772745367 29.0 2.0 34.0 2.0 35.0 92-93 21.23039754874167 29.0 2.0 34.0 2.0 35.0 94-95 20.25128968799956 27.5 2.0 34.0 2.0 35.0 96-97 19.025271306121873 25.5 2.0 33.0 2.0 35.0 98-99 17.507092676046604 22.5 2.0 32.5 2.0 34.0 100-101 13.856823833726049 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4247987794450161 1101 2 0.413666347137962 1101 3 0.3667147719051904 1101 4 0.21310223827930486 1101 5 0.24470787170341168 1101 6 0.34431505542632124 1101 7 0.2984914333091737 1101 8 0.43003869922289795 1101 9 0.5798095815514799 1101 10-11 0.6808075615592202 1101 12-13 0.66908257261111 1101 14-15 0.7163017539521661 1101 16-17 0.5562004966160714 1101 18-19 0.6650193940360865 1101 20-21 0.6330327401583205 1101 22-23 0.7160415508100542 1101 24-25 0.6939590065897576 1101 26-27 0.5990398307365155 1101 28-29 0.6497960212500971 1101 30-31 0.6657207198198805 1101 32-33 0.7704989130012834 1101 34-35 0.7132812958311519 1101 36-37 0.6646523397214281 1101 38-39 0.7397661538494091 1101 40-41 0.8350419627658923 1101 42-43 1.0362558725561968 1101 44-45 0.9161906013545078 1101 46-47 0.9609047022240489 1101 48-49 0.7890254649387742 1101 50-51 0.8190303355835127 1101 52-53 0.8471248383740502 1101 54-55 0.8744499488200361 1101 56-57 1.0178268874667253 1101 58-59 0.9710777071666001 1101 60-61 1.1095467251488316 1101 62-63 0.891806304466531 1101 64-65 0.7891996808637209 1101 66-67 0.866231572939558 1101 68-69 0.9183485140659293 1101 70-71 0.9538262283524226 1101 72-73 0.9569735364434173 1101 74-75 0.8815241734052606 1101 76-77 0.8492731795511475 1101 78-79 1.108312101109945 1101 80-81 1.1912268943679756 1101 82-83 1.0991728807984167 1101 84-85 1.073597689017788 1101 86-87 1.2272212370926 1101 88-89 1.0553249084711283 1101 90-91 0.8462376828737064 1101 92-93 0.6658419266651414 1101 94-95 0.7240517891775617 1101 96-97 0.8498770695652986 1101 98-99 0.9989203006189911 1101 100-101 0.7723478047216279 1102 1 0.5268642230684968 1102 2 0.31337274239069757 1102 3 0.3725856615764762 1102 4 0.381358316992646 1102 5 0.38824128396497315 1102 6 0.39154475034736436 1102 7 0.46916864922264523 1102 8 0.5818623304448636 1102 9 0.7044412842523968 1102 10-11 0.7691959862454354 1102 12-13 0.7436276935930692 1102 14-15 0.8008434605650621 1102 16-17 0.7839161855644647 1102 18-19 0.7863836850043882 1102 20-21 0.7587677851184864 1102 22-23 0.8351516780732169 1102 24-25 0.8125932658490171 1102 26-27 0.7108882251738038 1102 28-29 0.6952167720738132 1102 30-31 0.6630239622824448 1102 32-33 0.7891359788271544 1102 34-35 0.612836902696948 1102 36-37 0.8092956802037605 1102 38-39 0.7191863585118199 1102 40-41 0.920360296292067 1102 42-43 0.9479741146192922 1102 44-45 1.0122184831302121 1102 46-47 0.9754075060357437 1102 48-49 1.0971078774427667 1102 50-51 1.1934065201956727 1102 52-53 1.0371630162890213 1102 54-55 0.9122899996619367 1102 56-57 1.0354526853581163 1102 58-59 1.0135574172396602 1102 60-61 0.9781613126145317 1102 62-63 1.0731618175964464 1102 64-65 1.119858382046683 1102 66-67 0.7222435141056565 1102 68-69 0.8557245860710658 1102 70-71 0.9792181662401767 1102 72-73 1.0300841661336548 1102 74-75 1.0561577442808812 1102 76-77 1.0906971815154023 1102 78-79 1.1115397388004453 1102 80-81 1.1837390865754323 1102 82-83 1.0679884821406098 1102 84-85 1.072240980944791 1102 86-87 1.3265030143138858 1102 88-89 1.245215827730167 1102 90-91 0.9633226484924045 1102 92-93 1.0445165057834593 1102 94-95 0.8601572699372575 1102 96-97 1.07050983669534 1102 98-99 1.0978212112551553 1102 100-101 1.1936585643059665 1103 1 0.46330326627837337 1103 2 0.34911208021337004 1103 3 0.3986624011948834 1103 4 0.41700997243597016 1103 5 0.3416907087685672 1103 6 0.482787034297985 1103 7 0.3990841234089544 1103 8 0.46734037477707346 1103 9 0.5532593257787752 1103 10-11 0.5715423667168196 1103 12-13 0.694529945276571 1103 14-15 0.769803898275498 1103 16-17 0.8120973022872562 1103 18-19 0.767645960537493 1103 20-21 0.5520302710893077 1103 22-23 0.6343809499587394 1103 24-25 0.7245426205067034 1103 26-27 0.8178958009313746 1103 28-29 0.7469318394138824 1103 30-31 0.8181560119457387 1103 32-33 0.8484706449326538 1103 34-35 0.8265320135274763 1103 36-37 0.91246715186481 1103 38-39 0.8860546946509871 1103 40-41 0.8949587109946506 1103 42-43 1.0458188817348955 1103 44-45 0.9845897653973239 1103 46-47 0.7617869252276677 1103 48-49 0.8079050856358094 1103 50-51 1.1522834842809644 1103 52-53 1.1086899172430051 1103 54-55 0.932075528562045 1103 56-57 0.9730359836184945 1103 58-59 0.8979218953541377 1103 60-61 0.9493532668793989 1103 62-63 1.0678658018837588 1103 64-65 1.2650209451779908 1103 66-67 1.167091297495105 1103 68-69 1.2825580246232526 1103 70-71 1.318733107317616 1103 72-73 1.2412847132717033 1103 74-75 1.3754925857511395 1103 76-77 1.5067982968352673 1103 78-79 1.2694820087218837 1103 80-81 1.3868772389323354 1103 82-83 1.4212607885379143 1103 84-85 1.4202961857708303 1103 86-87 1.4617180115080473 1103 88-89 1.5406353002329745 1103 90-91 1.4028744161691726 1103 92-93 1.460670230530937 1103 94-95 1.545579197546683 1103 96-97 1.7169613308030875 1103 98-99 1.7082747045211484 1103 100-101 1.5972079470220155 1104 1 0.31599336358190655 1104 2 0.35409826897648955 1104 3 0.328074513750213 1104 4 0.29980070186801555 1104 5 0.3352436867955575 1104 6 0.38336274127419756 1104 7 0.26195295902810756 1104 8 0.3609243526449575 1104 9 0.45045888489215 1104 10-11 0.4943456759269722 1104 12-13 0.5505073364250634 1104 14-15 0.7100180989459659 1104 16-17 0.7362565878216927 1104 18-19 0.5934768446700289 1104 20-21 0.6370278264607521 1104 22-23 0.6650835947298219 1104 24-25 0.6810584090884007 1104 26-27 0.6184484028147779 1104 28-29 0.7499666689696269 1104 30-31 0.6966114883099621 1104 32-33 0.7147926707920718 1104 34-35 0.5619630789648724 1104 36-37 0.7830944063640572 1104 38-39 0.8479847106050826 1104 40-41 0.8395163947792028 1104 42-43 0.8587045889488678 1104 44-45 0.5539104543314508 1104 46-47 0.49854889582994133 1104 48-49 0.4347478539551304 1104 50-51 0.6183212077648292 1104 52-53 0.6164887243130366 1104 54-55 0.7267566020770886 1104 56-57 0.7418159585138042 1104 58-59 0.7221868607787982 1104 60-61 0.7864004847884729 1104 62-63 0.9381211040148578 1104 64-65 0.9501972144797399 1104 66-67 0.9155057181211887 1104 68-69 1.0062641699073467 1104 70-71 1.0220920635282837 1104 72-73 1.088301412949999 1104 74-75 1.047310194514246 1104 76-77 0.8074309130572352 1104 78-79 1.0408349399290628 1104 80-81 1.0910345096295302 1104 82-83 0.9900596315763046 1104 84-85 1.1288777159619947 1104 86-87 1.3065883456052525 1104 88-89 1.2145002092056139 1104 90-91 1.1540430827370791 1104 92-93 1.3466189353959912 1104 94-95 1.5466543616446593 1104 96-97 1.6899859825349637 1104 98-99 1.8120247250962649 1104 100-101 1.825960101312825 1105 1 0.2826021248854076 1105 2 0.1687841679672175 1105 3 0.24508522481896478 1105 4 0.14128363145805878 1105 5 0.1165726518894985 1105 6 0.2513346699839829 1105 7 0.19621158248610016 1105 8 0.25808852716044584 1105 9 0.4265652360343708 1105 10-11 0.4567069051832391 1105 12-13 0.4777433688201782 1105 14-15 0.5592073669242481 1105 16-17 0.4603116258160398 1105 18-19 0.4158549900358324 1105 20-21 0.39140012264469703 1105 22-23 0.3580841771997001 1105 24-25 0.515368859839235 1105 26-27 0.5026640812185263 1105 28-29 0.5123573206412502 1105 30-31 0.6516164020098998 1105 32-33 0.48997346135685405 1105 34-35 0.5226109898737477 1105 36-37 0.5596478159479901 1105 38-39 0.5519246097347477 1105 40-41 0.6924329290663493 1105 42-43 0.7282141128722728 1105 44-45 0.6688233486089032 1105 46-47 0.5475216307736872 1105 48-49 0.43767500707239293 1105 50-51 0.6453959506638753 1105 52-53 0.49652000004164165 1105 54-55 0.48056884749561846 1105 56-57 0.562965678894102 1105 58-59 0.670331526254337 1105 60-61 0.6920223582120144 1105 62-63 0.7926543053672255 1105 64-65 0.969490959789514 1105 66-67 0.9244949990811975 1105 68-69 0.9475610422206096 1105 70-71 1.088958950656668 1105 72-73 1.1492043181252711 1105 74-75 1.220168379326303 1105 76-77 1.2093068731646 1105 78-79 1.2141832136432136 1105 80-81 1.321959190523721 1105 82-83 1.36905635425801 1105 84-85 1.371654633984626 1105 86-87 1.497892121581529 1105 88-89 1.4018152337399492 1105 90-91 1.324401002790097 1105 92-93 1.2275370115486588 1105 94-95 1.3015389523271956 1105 96-97 1.4130614832711608 1105 98-99 1.572887816775772 1105 100-101 1.4444010520767563 1106 1 0.2341844479637949 1106 2 0.09203169381215659 1106 3 0.02880640974778359 1106 4 0.10786970168374665 1106 5 0.1998614673648902 1106 6 0.04325287620380891 1106 7 0.24116014387649898 1106 8 0.2174226929504144 1106 9 0.14541079238742327 1106 10-11 0.0583750412123365 1106 12-13 0.15075758558445784 1106 14-15 0.351219934615969 1106 16-17 0.21761555879126604 1106 18-19 0.1670448185519504 1106 20-21 0.2382773314678559 1106 22-23 0.23804307055676333 1106 24-25 0.09820601023749731 1106 26-27 0.14133823289387237 1106 28-29 0.24769967935576886 1106 30-31 0.03225856255167514 1106 32-33 0.26405072633260573 1106 34-35 -0.15198374740534604 1106 36-37 0.07302918760055377 1106 38-39 0.10581607137346083 1106 40-41 0.3786455299689422 1106 42-43 -0.11781158949936099 1106 44-45 0.054008037633913375 1106 46-47 0.22375417458417246 1106 48-49 0.45497278948221265 1106 50-51 0.2342990688803681 1106 52-53 0.19102439338067967 1106 54-55 0.2551478942781529 1106 56-57 -0.4906003653965243 1106 58-59 -0.07844832938763346 1106 60-61 0.39619429601155787 1106 62-63 0.17460776262953814 1106 64-65 0.2614668616994713 1106 66-67 -0.07812964272756773 1106 68-69 -0.044132758490142976 1106 70-71 -0.17291079391461395 1106 72-73 0.21632256793436255 1106 74-75 0.4545200791437516 1106 76-77 0.4207423228168139 1106 78-79 0.5686431382851325 1106 80-81 0.736102950977223 1106 82-83 0.6162024318250516 1106 84-85 0.673564641271355 1106 86-87 0.39471993743163836 1106 88-89 0.3771138311269979 1106 90-91 0.5890943539524258 1106 92-93 0.5020987154846956 1106 94-95 0.42188239804217176 1106 96-97 0.5657646321858536 1106 98-99 0.755507717296112 1106 100-101 0.3530719837180829 1107 1 0.22668887684183403 1107 2 0.11444060645776233 1107 3 0.19989032169862853 1107 4 0.1487079080742717 1107 5 0.12778387052732398 1107 6 0.28104562302515745 1107 7 0.2863798435055287 1107 8 0.18857072806280684 1107 9 0.4483608420198024 1107 10-11 0.42403954708940006 1107 12-13 0.4122060095980018 1107 14-15 0.5340927687943946 1107 16-17 0.5862329061619391 1107 18-19 0.5799654499070357 1107 20-21 0.6236732526098194 1107 22-23 0.5266749061504541 1107 24-25 0.5755023833407975 1107 26-27 0.47364246226909046 1107 28-29 0.6719925348419906 1107 30-31 0.7332465420522425 1107 32-33 0.7632465614391393 1107 34-35 0.6876790854040422 1107 36-37 0.7079922742003717 1107 38-39 0.7668072448657313 1107 40-41 0.7275436604592542 1107 42-43 0.8868436470141887 1107 44-45 0.8658979663274735 1107 46-47 0.7951563779261903 1107 48-49 0.7445617660358295 1107 50-51 0.6885679655867492 1107 52-53 0.8853355342773384 1107 54-55 1.0059056348735211 1107 56-57 0.9681034284995746 1107 58-59 0.9244734558513912 1107 60-61 0.8603774377544795 1107 62-63 0.9713910326187865 1107 64-65 1.0186898741664088 1107 66-67 1.0936781360894017 1107 68-69 1.1792176453244103 1107 70-71 1.3252836210566308 1107 72-73 1.2810298752213356 1107 74-75 1.2560693316100995 1107 76-77 1.372277244582584 1107 78-79 1.3478887171871676 1107 80-81 1.4584333009037316 1107 82-83 1.4391685256948534 1107 84-85 1.485356278519955 1107 86-87 1.7232642789116142 1107 88-89 1.579316878584411 1107 90-91 1.5832534088484849 1107 92-93 1.6859002277257922 1107 94-95 1.7991055936449634 1107 96-97 1.914747126831692 1107 98-99 1.777555929153813 1107 100-101 1.547008304436856 1108 1 0.3916631604053862 1108 2 0.16579548935055044 1108 3 0.25755029731416457 1108 4 0.2967157424465867 1108 5 0.36786995921067955 1108 6 0.33578183319482946 1108 7 0.33974594737912867 1108 8 0.37039558118060967 1108 9 0.40624402871350185 1108 10-11 0.4701050803095157 1108 12-13 0.43246274655270156 1108 14-15 0.5766239730865692 1108 16-17 0.5861582936770731 1108 18-19 0.5861491668467949 1108 20-21 0.48836913027923856 1108 22-23 0.3908007198717627 1108 24-25 0.5366626547656708 1108 26-27 0.6261823142403813 1108 28-29 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-1.5636524147973745 2316 76-77 -1.7625872883397946 2316 78-79 -1.8134093526782493 2316 80-81 -1.9222953136612695 2316 82-83 -1.8835701018192914 2316 84-85 -2.136242467259052 2316 86-87 -2.0860539858253517 2316 88-89 -2.3388572020665457 2316 90-91 -2.3564481326270617 2316 92-93 -2.616416425503086 2316 94-95 -2.9264120178655837 2316 96-97 -3.0849297605616925 2316 98-99 -3.20809766579859 2316 100-101 -2.926415927479052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12654.0 3 4161.0 4 4620.0 5 5206.0 6 6316.0 7 7807.0 8 9811.0 9 12636.0 10 16309.0 11 20020.0 12 24638.0 13 28812.0 14 32708.0 15 37669.0 16 42919.0 17 48844.0 18 47291.0 19 46283.0 20 48244.0 21 52653.0 22 58321.0 23 64970.0 24 72526.0 25 81476.0 26 91284.0 27 103561.0 28 118938.0 29 136863.0 30 157192.0 31 184478.0 32 217978.0 33 259996.0 34 319120.0 35 392538.0 36 463847.0 37 453199.0 38 209780.0 39 10897.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48425123276042 18.18392667795449 14.536435488140361 23.79538660114472 2 15.535136944125341 19.404186774859056 40.23581710989893 24.82485917111667 3 19.38829117930314 23.106861453190294 29.645899995848406 27.85894737165816 4 13.092093191088477 16.070588343835627 36.554959964162 34.28235850091389 5 13.995950693527698 37.008081948076885 34.247661983155155 14.748305375240262 6 33.17909190326016 35.65436834893086 16.93641621459297 14.230123533216016 7 29.28015272785186 32.241277641403464 20.09935196667067 18.379217664074005 8 26.820275954917854 34.67723537079698 19.429992577121507 19.07249609716366 9 26.356215212408024 16.796772484699794 19.80125289739243 37.04575940549975 10-11 25.203885852350876 26.14403951057919 27.264238395246398 21.387836241823532 12-13 26.289218631625694 23.29935422534959 27.390434216477676 23.02099292654704 14-15 22.642989447184455 25.02129049867648 25.613814166592842 26.72190588754622 16-17 22.565909812819857 28.095982555237285 26.570218767536012 22.76788886440684 18-19 22.706157313360446 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23.09308758734011 44-45 22.597118272479 26.5388473089916 28.224765940278544 22.63926847825085 46-47 23.117714066858447 26.77338375795261 27.390237936051925 22.718664239137016 48-49 22.710563867408805 26.91112748393452 27.312284413350874 23.066024235305804 50-51 22.912596256246413 27.052587654624393 27.401478147784058 22.63333794134513 52-53 23.324539163708646 26.54042504114947 27.511666429649477 22.623369365492405 54-55 22.57219440262972 26.879025733976427 27.71009339190808 22.838686471485772 56-57 22.73315180046267 26.981082417903142 27.43877617898226 22.846989602651924 58-59 22.729649142840174 26.55471111468414 27.401860205038346 23.313779537437345 60-61 22.206949243654705 26.851176537837233 27.918940810614938 23.02293340789312 62-63 22.48324742103087 26.823915834531242 27.946143418716552 22.74669332572134 64-65 22.137930266472633 26.92566698799357 27.715206997289055 23.221195748244742 66-67 22.078406660453403 26.87614858631641 27.71712785483341 23.328316898396775 68-69 22.86211893577675 26.37631263686068 27.85961453018618 22.901953897176387 70-71 22.534013561894774 26.568385349958035 27.374330214367248 23.523270873779943 72-73 22.46205907137127 26.69804395312329 27.560460573466152 23.279436402039288 74-75 22.534612065764843 27.09603039870129 27.393398422196785 22.975959113337073 76-77 22.648920298551104 26.579789398112435 27.481518502748447 23.289771800588017 78-79 22.759742557861188 26.87425753967223 27.35098090840985 23.01501899405673 80-81 23.020189232213227 26.853177679655854 27.053133418553237 23.073499669577686 82-83 22.872565851308373 26.90201095942101 27.27180917997107 22.953614009299546 84-85 22.760159120223218 27.099947022217265 27.19068379482712 22.9492100627324 86-87 23.011373782351942 27.022580003888848 26.818498867588424 23.14754734617079 88-89 22.81102400414433 27.143016824296147 26.644860120614176 23.401099050945348 90-91 22.732466855381027 27.063183632372915 26.878621856800294 23.325727655445764 92-93 22.98690820430975 26.600281198733143 27.144870094737495 23.267940502219606 94-95 22.67066079277103 26.37174516881304 27.381045674302012 23.57654836411392 96-97 22.998385355778723 25.943388857802468 27.38915486627647 23.669070920142335 98-99 22.682258800852836 26.32278759583455 26.938589328697887 24.056364274614722 100-101 23.313493296062124 25.654629648723876 27.335484574199175 23.696392481014826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 290.0 1 212.5 2 198.5 3 288.0 4 268.0 5 267.0 6 446.0 7 976.0 8 1612.5 9 1942.0 10 1829.0 11 1622.0 12 1706.0 13 1922.0 14 2184.0 15 2482.0 16 2765.5 17 3046.5 18 3531.5 19 4204.0 20 4935.5 21 5881.5 22 7234.0 23 9153.0 24 11560.0 25 14586.0 26 19111.0 27 24981.5 28 30378.0 29 35661.5 30 41413.5 31 51068.0 32 65927.5 33 71754.0 34 71405.5 35 76294.0 36 83994.5 37 92352.5 38 98312.0 39 102822.5 40 109911.5 41 118416.5 42 122896.5 43 126461.0 44 131917.5 45 140455.5 46 145384.5 47 148207.5 48 150818.0 49 147929.5 50 144372.5 51 142036.5 52 139606.0 53 139510.5 54 140243.5 55 133055.0 56 122174.5 57 111769.0 58 104327.0 59 92578.0 60 73935.0 61 61390.5 62 52824.5 63 40272.5 64 28354.5 65 21483.5 66 16393.0 67 12593.0 68 9560.0 69 7251.0 70 5609.0 71 3911.5 72 2713.5 73 2110.5 74 1648.0 75 1225.5 76 892.0 77 601.5 78 418.5 79 299.0 80 183.0 81 103.0 82 70.5 83 51.0 84 33.0 85 23.0 86 13.5 87 12.5 88 11.0 89 9.0 90 7.0 91 4.0 92 3.0 93 2.0 94 1.0 95 2.0 96 2.5 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007448999312695425 2 0.10582186652468345 3 0.11383402042459297 4 0.11675218510379323 5 0.15727371744742505 6 0.09750253739538443 7 0.027031420186276175 8 0.027440987158795515 9 0.022986946332647733 10-11 0.05095269117498365 12-13 0.05393485069364007 14-15 0.02731299747988322 16-17 0.03096070332888356 18-19 0.03593950183857174 20-21 0.059003241978566844 22-23 0.03148546101242396 24-25 0.04964719645007827 26-27 0.05690421124440525 28-29 0.05371726823948917 30-31 0.06809050918133962 32-33 0.04963439748218704 34-35 0.061703824203616225 36-37 0.05293653119812418 38-39 0.03135747133351167 40-41 0.03709140894878237 42-43 0.032918945416241636 44-45 0.007602586927390176 46-47 0.013733292547288986 48-49 0.009292050689032437 50-51 0.008319329129299015 52-53 0.009688818693660544 54-55 0.011391081423194033 56-57 0.014565225460218888 58-59 0.019428833258886002 60-61 0.024945188420005808 62-63 0.011519071102106326 64-65 0.00938164346427104 66-67 0.010354365024004464 68-69 0.011480674198432638 70-71 0.03099910023255725 72-73 0.027799358259749937 74-75 0.0290536571130904 76-77 0.02308933807577757 78-79 0.005413963417989973 80-81 0.005132386124382929 82-83 0.006284293234593562 84-85 0.005388365482207515 86-87 0.013323725574769652 88-89 0.037590568696540316 90-91 0.035030775118294465 92-93 0.029232842663567613 94-95 0.04686982041768152 96-97 0.043004532114530286 98-99 0.025751523397153253 100-101 0.0749123590673648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3906565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.10833991389285 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22512949715417 34.74631080174022 2 14.27958511623737 13.739342692026248 3 5.390161250177311 7.779351288426711 4 2.641502379330235 5.083131773927027 5 1.4722241076143998 3.541312889927056 6 0.9369268004898207 2.704439579544022 7 0.6015730239930701 2.025847566490093 8 0.43606150802467747 1.678255620113272 9 0.3067081280793949 1.3279696991997583 >10 1.5499830941041992 13.143418515788849 >50 0.10072613715705991 3.3394652386229065 >100 0.051246191423571966 4.58796793447811 >500 0.0044335921690756355 1.4506477348303959 >1k 0.0033652566411332195 3.664677905230343 >5k 3.739174045703576E-4 1.187860759655045 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8885 0.22743765942714378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6890 0.17636977754113908 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6676 0.17089181928369296 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6499 0.1663609846501978 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6343 0.16236770666813427 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5717 0.14634339886831527 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5214 0.13346763716973864 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4964 0.12706815322412401 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4887 0.1250971121688747 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4867 0.12458515345322553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4651 0.11905599932421448 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4531 0.11598424703031947 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4079 0.10441398005664823 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5597935782458503E-5 2 0.0 0.0 0.0 0.0 2.5597935782458503E-5 3 0.0 0.0 0.0 0.0 2.5597935782458503E-5 4 0.0 0.0 0.0 0.0 2.5597935782458503E-5 5 0.0 0.0 0.0 0.0 2.5597935782458503E-5 6 0.0 0.0 0.0 0.0 2.5597935782458503E-5 7 0.0 0.0 0.0 0.0 2.5597935782458503E-5 8 0.0 0.0 0.0 0.0 2.5597935782458503E-5 9 0.0 0.0 0.0 2.5597935782458503E-5 2.5597935782458503E-5 10-11 0.0 0.0 0.0 5.1195871564917006E-5 2.5597935782458503E-5 12-13 0.0 0.0 0.0 5.1195871564917006E-5 1.9198451836843877E-4 14-15 3.8396903673687756E-5 0.0 0.0 1.0239174312983401E-4 2.0478348625966802E-4 16-17 5.1195871564917006E-5 0.0 0.0 1.0239174312983401E-4 2.0478348625966802E-4 18-19 7.679380734737551E-5 0.0 0.0 1.0239174312983401E-4 2.0478348625966802E-4 20-21 1.0239174312983401E-4 1.2798967891229251E-5 0.0 1.1519071102106327E-4 2.687783257158143E-4 22-23 1.0239174312983401E-4 2.5597935782458503E-5 0.0 2.8157729360704355E-4 3.3277316517196055E-4 24-25 1.5358761469475102E-4 2.5597935782458503E-5 0.0 6.399483945614626E-4 3.5837110095441905E-4 26-27 1.5358761469475102E-4 2.5597935782458503E-5 0.0 0.0013438916285790714 3.5837110095441905E-4 28-29 2.431803899333558E-4 2.5597935782458503E-5 0.0 0.0033149326838283763 3.8396903673687755E-4 30-31 2.8157729360704355E-4 2.5597935782458503E-5 0.0 0.011557468005780015 3.8396903673687755E-4 32-33 2.8157729360704355E-4 2.5597935782458503E-5 0.0 0.02872088394791844 4.0956697251933605E-4 34-35 2.8157729360704355E-4 2.5597935782458503E-5 0.0 0.0565330411755596 4.3516490830179455E-4 36-37 2.8157729360704355E-4 2.5597935782458503E-5 0.0 0.10395321721256398 4.3516490830179455E-4 38-39 3.0717522938950205E-4 2.5597935782458503E-5 0.0 0.17709931871093915 4.6076284408425305E-4 40-41 3.0717522938950205E-4 2.5597935782458503E-5 0.0 0.2551090280079814 4.6076284408425305E-4 42-43 3.455721330631898E-4 2.5597935782458503E-5 0.0 0.33672804625035035 4.6076284408425305E-4 44-45 3.8396903673687755E-4 2.5597935782458503E-5 0.0 0.438595031696644 4.8636077986671154E-4 46-47 4.991597477579408E-4 2.5597935782458503E-5 0.0 0.562425046044287 4.8636077986671154E-4 48-49 5.119587156491701E-4 2.5597935782458503E-5 0.0 0.6923473691081552 5.247576835403993E-4 50-51 5.375566514316286E-4 2.5597935782458503E-5 0.0 0.8347870827696455 5.887525229965456E-4 52-53 5.375566514316286E-4 2.5597935782458503E-5 0.0 0.9758445078988831 6.655463303439211E-4 54-55 5.375566514316286E-4 2.5597935782458503E-5 0.0 1.1282674165155322 6.655463303439211E-4 56-57 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.3076321525432189 6.783452982351503E-4 58-59 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.4997702585263526 7.039432340176088E-4 60-61 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.7113755946720457 7.423401376912966E-4 62-63 5.887525229965456E-4 2.5597935782458503E-5 0.0 1.9183221065053315 7.935360092562136E-4 64-65 5.887525229965456E-4 2.5597935782458503E-5 0.0 2.135597385426839 7.935360092562136E-4 66-67 6.015514908877748E-4 2.5597935782458503E-5 0.0 2.392293485453333 8.063349771474428E-4 68-69 6.399483945614626E-4 2.5597935782458503E-5 0.0 2.6727444698859486 8.191339450386721E-4 70-71 6.399483945614626E-4 2.5597935782458503E-5 0.0 2.967389509709937 8.703298166035891E-4 72-73 6.655463303439211E-4 2.5597935782458503E-5 0.0 3.2767661615767305 9.343246560597353E-4 74-75 6.911442661263796E-4 2.5597935782458503E-5 0.0 3.587909071012514 9.599225918421938E-4 76-77 6.911442661263796E-4 3.8396903673687756E-5 0.0 3.9215525660010777 9.727215597334231E-4 78-79 7.039432340176088E-4 5.1195871564917006E-5 0.0 4.2979958096179125 9.727215597334231E-4 80-81 8.191339450386721E-4 5.1195871564917006E-5 0.0 4.690732139360282 9.855205276246524E-4 82-83 8.191339450386721E-4 5.1195871564917006E-5 0.0 5.099351988255667 0.0010239174312983402 84-85 8.191339450386721E-4 5.1195871564917006E-5 0.0 5.518172614560362 0.0010367163991895694 86-87 8.191339450386721E-4 5.1195871564917006E-5 0.0 5.950918517930715 0.0011007112386457157 88-89 8.191339450386721E-4 5.1195871564917006E-5 0.0 6.407368109835623 0.0011519071102106327 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6530 0.0 45.302814 1 GTATCAA 10920 0.0 40.89695 1 ATCAACG 14085 0.0 31.30191 3 TCAACGC 14325 0.0 30.976685 4 TATCAAC 14280 0.0 30.841162 2 CAACGCA 14550 0.0 30.26885 5 AACGCAG 15070 0.0 29.32883 6 ACGCAGA 17110 0.0 25.60782 7 CGCAGAG 17155 0.0 25.374437 8 TAAGGTG 2745 0.0 23.91028 5 GTACATG 12290 0.0 22.948313 1 TACATGG 12030 0.0 22.811726 2 GTGTAAG 1510 0.0 22.677935 1 GCAGAGT 18935 0.0 22.109554 9 GTAAGGT 2760 0.0 21.712479 4 ACATGGG 12040 0.0 21.291693 3 GGTAAGG 2890 0.0 21.229504 3 AGGTAAG 2790 0.0 20.967607 2 AGAGTAC 16425 0.0 20.75802 10-11 GTGTAGC 2590 0.0 20.566774 1 >>END_MODULE