FastQCFastQC Report
Thu 26 May 2016
SRR938567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906565
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.22052621676588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73380.1878376527716805No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65410.16743609795306105No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA64230.16441554153073096No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62870.1609342222643166No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC59470.1522309240982807No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57600.14744411010696096No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG54200.13874081194092508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49910.1277592974902504No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA46980.12025910230599003No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45470.11639381400283882No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44380.11360363900255084No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA42050.10763931996523801No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41300.10571947478155361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.048.1744771
GTATCAA111450.038.8554271
ATCAACG138350.030.8377383
TCAACGC141400.030.1725654
TATCAAC143450.029.889172
CAACGCA143000.029.8349725
AACGCAG150100.028.4728076
TAAGGTG30850.026.3345725
GTAAGGT29450.025.8119034
ACGCAGA170300.024.9282267
CGCAGAG173500.024.4684548
AGGTAAG30050.024.3603822
GGTAAGG32250.023.1289163
AAGGTAA30850.022.3422161
TACATGG116450.022.0833842
GTACATG119200.021.6539421
GCAGAGT193550.021.5166639
ACATGGG114450.021.1294063
GAGTACT118650.020.51136812-13
AGAGTAC165900.020.40852510-11
Produced by FastQC (version 0.11.5)