##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3906565 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83487821142103 31.0 31.0 34.0 30.0 34.0 2 32.0599777553938 33.0 31.0 34.0 30.0 34.0 3 32.1242982005931 33.0 31.0 34.0 30.0 34.0 4 35.78980971774436 37.0 35.0 37.0 35.0 37.0 5 35.68284029575855 37.0 35.0 37.0 33.0 37.0 6 35.683514033428345 37.0 35.0 37.0 33.0 37.0 7 35.62342902268361 37.0 35.0 37.0 33.0 37.0 8 35.59678029163728 37.0 35.0 37.0 33.0 37.0 9 37.19223998576755 39.0 37.0 39.0 33.0 39.0 10-11 37.098187026198204 39.0 37.0 39.0 33.0 39.0 12-13 36.992318315451044 39.0 37.0 39.0 33.0 39.0 14-15 38.06587884752973 40.0 37.0 41.0 33.0 41.0 16-17 37.978834090818914 40.0 37.0 41.0 33.0 41.0 18-19 38.206282245399734 40.0 37.5 41.0 33.0 41.0 20-21 38.168539368985286 40.0 37.0 41.0 33.0 41.0 22-23 38.070894123097915 40.0 37.0 41.0 33.0 41.0 24-25 37.94689746618833 40.0 37.0 41.0 33.0 41.0 26-27 37.75892017667695 40.0 37.0 41.0 32.0 41.0 28-29 37.65372213184729 40.0 37.0 41.0 32.0 41.0 30-31 37.40536315151546 39.0 36.0 41.0 31.5 41.0 32-33 37.21130264055506 39.0 36.0 41.0 31.0 41.0 34-35 37.125506807131075 39.0 36.0 41.0 31.0 41.0 36-37 37.07689799094601 39.0 36.0 41.0 31.0 41.0 38-39 36.912205223770755 39.0 36.0 41.0 30.5 41.0 40-41 36.749987137037266 39.0 35.0 41.0 30.0 41.0 42-43 36.5422593762039 39.0 35.0 41.0 30.0 41.0 44-45 36.268964934667665 39.0 35.0 40.0 30.0 41.0 46-47 35.92470546375141 38.5 35.0 40.0 28.5 41.0 48-49 35.58786734637719 38.0 34.5 40.0 28.0 41.0 50-51 35.21933092115452 38.0 34.0 40.0 27.0 41.0 52-53 34.73518564262978 38.0 33.5 40.0 26.0 41.0 54-55 34.40221601330069 38.0 33.0 40.0 25.0 41.0 56-57 34.07206522865996 37.0 33.0 40.0 24.0 41.0 58-59 33.59950967154009 37.0 32.5 39.5 23.0 41.0 60-61 33.20438761418279 36.0 32.0 39.0 22.5 40.5 62-63 32.73449065867328 36.0 31.0 39.0 21.5 40.0 64-65 32.225804511124224 35.0 31.0 38.5 20.0 40.0 66-67 31.726641691613988 35.0 30.5 38.0 18.5 40.0 68-69 31.236713071457917 34.5 30.0 37.5 17.0 40.0 70-71 30.675101015854082 34.0 29.0 37.0 12.5 39.0 72-73 30.1890756969358 34.0 29.0 36.0 9.0 39.0 74-75 29.61256359487171 34.0 28.0 36.0 7.5 38.5 76-77 28.376640731691396 31.5 26.5 34.5 7.0 36.5 78-79 29.075684264820886 33.5 28.5 35.0 4.0 37.0 80-81 28.720000563154585 33.0 27.5 35.0 2.0 37.0 82-83 28.290291982854505 33.0 27.0 35.0 2.0 36.0 84-85 27.7536522494826 33.0 26.0 35.0 2.0 36.0 86-87 27.172320056110678 32.5 25.5 35.0 2.0 35.0 88-89 26.439283104210475 32.0 24.5 34.0 2.0 35.0 90-91 25.85796409377548 32.0 23.5 34.0 2.0 35.0 92-93 25.061893120938727 31.0 20.0 34.0 2.0 35.0 94-95 24.281516242530202 31.0 16.5 34.0 2.0 35.0 96-97 23.253570336088096 30.5 2.0 34.0 2.0 35.0 98-99 21.920769397155816 30.0 2.0 34.0 2.0 35.0 100-101 17.95248280778638 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2457423259253595 1101 2 0.311158723174529 1101 3 0.3048888999464978 1101 4 0.23810858714556105 1101 5 0.05486626581659948 1101 6 0.13041100121139948 1101 7 0.21947333150097847 1101 8 0.23396172046605557 1101 9 0.28273966480036705 1101 10-11 0.27132723755438803 1101 12-13 0.3744875670729968 1101 14-15 0.42849777067154093 1101 16-17 0.41000567933921417 1101 18-19 0.3929780484298959 1101 20-21 0.3561125986180471 1101 22-23 0.29824043347608153 1101 24-25 0.3252397404641627 1101 26-27 0.2517735368568026 1101 28-29 0.0634655729282656 1101 30-31 0.3302401836447615 1101 32-33 0.3393734553376788 1101 34-35 0.43417034119713804 1101 36-37 0.4095699419444472 1101 38-39 0.5088205906160965 1101 40-41 0.529087589699472 1101 42-43 0.5552890808492918 1101 44-45 0.5222711950061765 1101 46-47 0.4596185550691132 1101 48-49 0.42560362756684356 1101 50-51 0.5418409744218948 1101 52-53 0.5292578590855541 1101 54-55 0.502842631899135 1101 56-57 0.461138418306156 1101 58-59 0.5584671636005893 1101 60-61 0.4876353153844377 1101 62-63 0.6837767826300905 1101 64-65 0.6643302447701132 1101 66-67 0.5274676009332033 1101 68-69 0.6150697727861036 1101 70-71 0.5933911449006466 1101 72-73 0.738406335611721 1101 74-75 0.6501834903956656 1101 76-77 0.7848321171071149 1101 78-79 0.8989484208676224 1101 80-81 0.8053496520239669 1101 82-83 0.70325130826334 1101 84-85 0.908833185644518 1101 86-87 0.8877377515380154 1101 88-89 1.0120697900926636 1101 90-91 0.953782682491358 1101 92-93 1.244788720613677 1101 94-95 1.1224612239978597 1101 96-97 1.1961829480603043 1101 98-99 1.4952382156103425 1101 100-101 1.563426776820176 1102 1 0.34107640033002795 1102 2 0.3514524105691592 1102 3 0.34053066014242717 1102 4 0.26547545257913185 1102 5 0.18889574819618105 1102 6 0.2318472057140326 1102 7 0.22592146132896573 1102 8 0.31117217577035916 1102 9 0.32787700405125264 1102 10-11 0.3982424609915256 1102 12-13 0.43964252742981813 1102 14-15 0.5899311376147978 1102 16-17 0.5074204010380043 1102 18-19 0.4782063738153042 1102 20-21 0.4869412176082122 1102 22-23 0.48859478058948724 1102 24-25 0.5519166554035024 1102 26-27 0.5544275243853107 1102 28-29 0.5062728248208899 1102 30-31 0.5381908855274702 1102 32-33 0.5396200027442077 1102 34-35 0.5059115213463414 1102 36-37 0.5880873361144978 1102 38-39 0.6540669124642022 1102 40-41 0.41746466759185097 1102 42-43 0.42363437566620377 1102 44-45 0.5051401190477449 1102 46-47 0.6018976214569847 1102 48-49 0.5951910175080997 1102 50-51 0.6864559048741441 1102 52-53 0.6244933162494206 1102 54-55 0.5705399886299389 1102 56-57 0.5592457421019859 1102 58-59 0.6292990936506158 1102 60-61 0.6457989030925688 1102 62-63 0.6463913353150232 1102 64-65 0.8172160667532182 1102 66-67 0.6871676116773493 1102 68-69 0.7011729979615886 1102 70-71 0.5536188676490532 1102 72-73 0.615396286037079 1102 74-75 0.7406251854379242 1102 76-77 0.7075266173385266 1102 78-79 0.9323460039144429 1102 80-81 1.0105436770330556 1102 82-83 0.922909266997511 1102 84-85 0.8079051763163854 1102 86-87 0.8543700420682825 1102 88-89 0.9172118850103992 1102 90-91 0.995398770695374 1102 92-93 1.2811468440227145 1102 94-95 1.2883582801677598 1102 96-97 1.1714515433719974 1102 98-99 1.4491744511057334 1102 100-101 1.4930240104064083 1103 1 0.29642853276549275 1103 2 0.1990604352605132 1103 3 0.24891134196060705 1103 4 0.1459906742401884 1103 5 0.05278639073265623 1103 6 0.11312526407542123 1103 7 0.15981771553772006 1103 8 0.13595647711716197 1103 9 0.2955222060714533 1103 10-11 0.27809568068849444 1103 12-13 0.31843917445562653 1103 14-15 0.47843422403455804 1103 16-17 0.35622090608850954 1103 18-19 0.4001032944101439 1103 20-21 0.39484841457790054 1103 22-23 0.3712143793549245 1103 24-25 0.454273557760402 1103 26-27 0.5352733086165173 1103 28-29 0.5285178444617458 1103 30-31 0.3985698487710181 1103 32-33 0.4561200728901156 1103 34-35 0.5280250174181731 1103 36-37 0.5776575170909268 1103 38-39 0.5232667230702646 1103 40-41 0.5461911687141878 1103 42-43 0.6439144331858415 1103 44-45 0.5661319821229398 1103 46-47 0.6119723268441888 1103 48-49 0.5202225831646672 1103 50-51 0.7306522081850275 1103 52-53 0.6332396335894899 1103 54-55 0.6819799086636067 1103 56-57 0.7134878156148972 1103 58-59 0.7085245075114486 1103 60-61 0.7343274174023335 1103 62-63 0.8649572023183936 1103 64-65 0.8588213557543369 1103 66-67 0.7759197160544566 1103 68-69 0.8125822291624907 1103 70-71 0.5562449549105573 1103 72-73 0.602397373298583 1103 74-75 0.7486919643380396 1103 76-77 0.7654583653744425 1103 78-79 1.0019526607606828 1103 80-81 0.9186613117131905 1103 82-83 1.062582913181572 1103 84-85 1.1188356617260382 1103 86-87 1.148303298241121 1103 88-89 1.0497262649206114 1103 90-91 1.134528786127472 1103 92-93 1.3371057385232774 1103 94-95 1.3064164661946975 1103 96-97 1.461211118287821 1103 98-99 1.682926906212355 1103 100-101 1.942479502830654 1104 1 0.20278036053683834 1104 2 0.2239345307936702 1104 3 0.17813888810830747 1104 4 0.174676652862793 1104 5 0.07534738604397262 1104 6 0.029641656193838628 1104 7 0.15561902027438634 1104 8 0.148177181353077 1104 9 0.11086877331916156 1104 10-11 0.1749642852567206 1104 12-13 0.19588426023405248 1104 14-15 0.33473380625301985 1104 16-17 0.30889516817341445 1104 18-19 0.26992781458320536 1104 20-21 0.2756947193785848 1104 22-23 0.3036266485859471 1104 24-25 0.30347076247091564 1104 26-27 0.2937046099819085 1104 28-29 0.32516041233371595 1104 30-31 0.3429550064007856 1104 32-33 0.42162055724357117 1104 34-35 0.37442004378785043 1104 36-37 0.36242283274978604 1104 38-39 0.35955898664388997 1104 40-41 0.4169480464419095 1104 42-43 0.500687547880375 1104 44-45 0.5820376133644416 1104 46-47 0.4946738212377042 1104 48-49 0.5503975905259892 1104 50-51 0.6202618357540999 1104 52-53 0.5358358931249114 1104 54-55 0.4632483691145737 1104 56-57 0.3382744440453749 1104 58-59 0.585233918599819 1104 60-61 0.6164708689374194 1104 62-63 0.590629414207335 1104 64-65 0.47110677253911604 1104 66-67 0.507901172845802 1104 68-69 0.698359322322343 1104 70-71 0.6695713963421959 1104 72-73 0.6239107904076882 1104 74-75 0.6525930672252009 1104 76-77 0.6179037712102158 1104 78-79 0.8296910867247114 1104 80-81 0.8408418807568765 1104 82-83 0.9414607128451777 1104 84-85 0.9914867455621668 1104 86-87 1.0970538095708449 1104 88-89 1.132255949478182 1104 90-91 1.191722273295614 1104 92-93 1.358879545151332 1104 94-95 1.42979766181924 1104 96-97 1.5562034552003077 1104 98-99 1.5277704926592364 1104 100-101 1.6148886842702517 1105 1 0.14861920256622696 1105 2 0.17201058666978497 1105 3 0.18169833135137026 1105 4 0.11458119157283875 1105 5 0.07298576391945488 1105 6 0.08909301284191429 1105 7 0.1318493526636928 1105 8 0.18314217052927262 1105 9 0.192034498810159 1105 10-11 0.2009563629831348 1105 12-13 0.29527779123135645 1105 14-15 0.3261951138551922 1105 16-17 0.2997640418486256 1105 18-19 0.25615066539461395 1105 20-21 0.26186369314978464 1105 22-23 0.22086788506188526 1105 24-25 0.23037577490035943 1105 26-27 0.14936495887168633 1105 28-29 0.2050247902296931 1105 30-31 0.22935145942683732 1105 32-33 0.3120747983416834 1105 34-35 0.40031447382712315 1105 36-37 0.3438771028859904 1105 38-39 0.19763620404351911 1105 40-41 0.1891551375429401 1105 42-43 0.21108906207430778 1105 44-45 0.23061618473607126 1105 46-47 0.20593872033888516 1105 48-49 0.3169000496534551 1105 50-51 0.4004807345387107 1105 52-53 0.3014865683458581 1105 54-55 0.2266999073930407 1105 56-57 0.1847862295233682 1105 58-59 0.39035724791337145 1105 60-61 0.36903931626517306 1105 62-63 0.25930953063299 1105 64-65 0.19254909435629486 1105 66-67 0.20259607079685082 1105 68-69 0.22162416847172395 1105 70-71 0.18277625321858793 1105 72-73 0.2973248980217029 1105 74-75 0.30775481506755753 1105 76-77 0.35665201042028727 1105 78-79 0.5479802850115796 1105 80-81 0.4813082210582138 1105 82-83 0.5075938188843026 1105 84-85 0.5579979872877345 1105 86-87 0.46550572078945507 1105 88-89 0.4423090635568734 1105 90-91 0.59961730231662 1105 92-93 0.7374164981107683 1105 94-95 0.8536617174493841 1105 96-97 1.0768525959576003 1105 98-99 1.0281347498478723 1105 100-101 1.4677439396516938 1106 1 -0.017631235784463684 1106 2 0.05125808192576642 1106 3 0.04511508514194418 1106 4 0.04499431729455239 1106 5 -0.014826803335289185 1106 6 -0.04411575242389176 1106 7 0.02189734455325265 1106 8 0.06056648197729686 1106 9 -0.15186595379716294 1106 10-11 0.05957843422700648 1106 12-13 0.06536613323337548 1106 14-15 0.10150822734131282 1106 16-17 0.1048929224129509 1106 18-19 0.040316072997939045 1106 20-21 0.1832636596111783 1106 22-23 0.18191182336832412 1106 24-25 0.16835461498893523 1106 26-27 0.21680118362623801 1106 28-29 0.23157689305665485 1106 30-31 0.10535119942070281 1106 32-33 0.12209975565370712 1106 34-35 0.17804104241248098 1106 36-37 0.24860772614692905 1106 38-39 0.12670491776692927 1106 40-41 0.15429335320164483 1106 42-43 0.04141573302342749 1106 44-45 0.05114510331809896 1106 46-47 -0.5351066354380052 1106 48-49 -0.33223941742134855 1106 50-51 0.08949174710968322 1106 52-53 -0.03767723879950324 1106 54-55 0.18899881230836257 1106 56-57 0.16128954023179887 1106 58-59 0.04742601462122309 1106 60-61 0.20328922586388387 1106 62-63 -0.020977224457617183 1106 64-65 0.07537385895787452 1106 66-67 0.043256756776077765 1106 68-69 -0.08447714051716915 1106 70-71 -0.004133816884749564 1106 72-73 0.36556674579138715 1106 74-75 0.45588607556202376 1106 76-77 0.40372752854807104 1106 78-79 0.5135004529668059 1106 80-81 -0.08986081242904831 1106 82-83 0.3627540121628172 1106 84-85 0.5693566641186756 1106 86-87 0.7274251724168437 1106 88-89 0.8413849629541588 1106 90-91 0.9254966256014789 1106 92-93 1.070116612710109 1106 94-95 1.1706230622929041 1106 96-97 1.2260531726686956 1106 98-99 1.0497110261448164 1106 100-101 0.9719199468750581 1107 1 0.10099187460252779 1107 2 0.13744354270645687 1107 3 0.123238086095661 1107 4 0.09692184774856827 1107 5 0.1003184547716458 1107 6 0.078418332936117 1107 7 0.11625540280607538 1107 8 0.13826058645002348 1107 9 0.13373165370582285 1107 10-11 0.14867136875619735 1107 12-13 0.16002076270921606 1107 14-15 0.25787240309999504 1107 16-17 0.26424509406476915 1107 18-19 0.22370008672365316 1107 20-21 0.21740075869573872 1107 22-23 0.28485075678256067 1107 24-25 0.293001005912771 1107 26-27 0.295883182511794 1107 28-29 0.23505186382855925 1107 30-31 0.1935319765339969 1107 32-33 0.23566082460871485 1107 34-35 0.24530527197212137 1107 36-37 0.30513183841509317 1107 38-39 0.28655382940848995 1107 40-41 0.387006065173388 1107 42-43 0.3921232905533856 1107 44-45 0.31237703001867345 1107 46-47 0.2555162902763328 1107 48-49 0.31196796356819334 1107 50-51 0.3270300247438911 1107 52-53 0.32006043023456954 1107 54-55 0.18817367643884353 1107 56-57 0.2748394673552568 1107 58-59 0.25175910492602327 1107 60-61 0.3518454750162405 1107 62-63 0.5257099952596818 1107 64-65 0.40193386585299606 1107 66-67 0.4314583703950312 1107 68-69 0.5689715573036835 1107 70-71 0.7388343106894801 1107 72-73 0.7379099152275259 1107 74-75 0.7054125797380983 1107 76-77 0.6751017876642997 1107 78-79 0.8670792412759738 1107 80-81 0.8854769035287795 1107 82-83 0.964759080799702 1107 84-85 1.126033848503564 1107 86-87 1.2184766808479424 1107 88-89 1.203002838857465 1107 90-91 1.2442684922463094 1107 92-93 1.4293272766979541 1107 94-95 1.5551173275153687 1107 96-97 1.6438877039196775 1107 98-99 1.7004896544726549 1107 100-101 1.6850126618973569 1108 1 0.14497479835323546 1108 2 0.12601825264811595 1108 3 0.16397527464073391 1108 4 0.11814937603406861 1108 5 0.08474600015353673 1108 6 0.1788268408964413 1108 7 0.1326759833734954 1108 8 0.20700681053631342 1108 9 0.24896593875056539 1108 10-11 0.2667736901686055 1108 12-13 0.30318775134563936 1108 14-15 0.30457208103197786 1108 16-17 0.3616165668439919 1108 18-19 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66-67 -0.9585743473869712 2316 68-69 -0.7953591309718497 2316 70-71 -0.7319703223398051 2316 72-73 -0.9953417134755931 2316 74-75 -1.0052151097444728 2316 76-77 -1.0378198216756722 2316 78-79 -1.1113124042587685 2316 80-81 -1.0443396249719576 2316 82-83 -1.012485861448603 2316 84-85 -1.1112133665686024 2316 86-87 -1.3514994493686459 2316 88-89 -1.27696061220486 2316 90-91 -1.1854842093241196 2316 92-93 -1.4483722662529743 2316 94-95 -1.2916475068692748 2316 96-97 -1.3626256200226017 2316 98-99 -1.3163526478917618 2316 100-101 -1.813624773010467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 25.0 4 48.0 5 143.0 6 254.0 7 689.0 8 1600.0 9 2983.0 10 4919.0 11 7623.0 12 10868.0 13 15137.0 14 19246.0 15 25131.0 16 30927.0 17 37892.0 18 36709.0 19 33469.0 20 33388.0 21 35280.0 22 38777.0 23 43891.0 24 50793.0 25 59534.0 26 70880.0 27 84655.0 28 101773.0 29 120951.0 30 143056.0 31 171343.0 32 206602.0 33 255723.0 34 325500.0 35 426474.0 36 561949.0 37 629670.0 38 305732.0 39 12924.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.178355088282636 18.951277651038872 15.135244860930788 23.735122399747706 2 15.89998601611374 20.0766859931146 39.09016123472081 24.93316675605085 3 19.314538475617326 23.314164745754905 30.065236339341595 27.306060439286178 4 13.534166204837241 16.709436551036525 36.142595861069765 33.61380138305647 5 13.932458927726321 37.153428682060955 34.32759069294801 14.586521697264718 6 33.235958760690984 36.00714080199377 16.609506461432606 14.147393975882643 7 29.221451582144415 32.313605430858054 20.03115268784725 18.433790299150278 8 26.859043686717104 34.92904892149497 19.22750549395697 18.984401897830956 9 26.157532256598827 16.6346137847444 19.654120691707423 37.55373326694935 10-11 25.1886119903291 26.26231740672432 27.214906189964843 21.33416441298174 12-13 26.116178791342264 23.335782202523188 27.462962986664756 23.085076019469792 14-15 22.456582701170976 25.110602281032058 25.555801580160576 26.87701343763639 16-17 22.470533576172418 28.164141643617857 26.636482434056518 22.728842346153204 18-19 22.630431082037543 26.627881527633612 28.666001973600846 22.075685416727993 20-21 22.757153663128605 25.903267960471666 29.00000383969037 22.339574536709357 22-23 22.583266885358363 25.86549820622465 28.937736860899538 22.613498047517446 24-25 22.63346220626795 26.086394998851215 28.882069424986927 22.398073369893908 26-27 22.07788850080296 26.506692893078437 28.609281287865628 22.806137318252976 28-29 22.320309346251086 26.993511684573722 28.34446972669824 22.341709242476952 30-31 22.41822912866905 26.383804407787544 28.28175896807752 22.916207495465883 32-33 21.98565792979942 26.41543725677062 28.325486253980177 23.273418559449784 34-35 22.890851439593792 26.582489446912888 28.112896687206845 22.413762426286475 36-37 23.073342465402686 25.93695031556847 28.233904727766184 22.75580249126266 38-39 22.406520090492815 26.41771969626669 28.146657237665234 23.02910297557526 40-41 22.991712583434126 26.25155349063426 28.21264422984622 22.544089696085397 42-43 22.675859699798465 26.344672442838352 27.967630434231687 23.011837423131496 44-45 22.584995543645206 26.512501409611005 28.303785795959495 22.598717250784293 46-47 23.072200257771215 26.79472887306368 27.450292008452436 22.68277886071267 48-49 22.695309050278187 26.93446800450012 27.342038338028424 23.028184607193275 50-51 22.901856846084012 27.033616802167952 27.456394636332604 22.608131715415432 52-53 23.383419961014344 26.559727023612815 27.48455484549726 22.57229816987558 54-55 22.607059360793713 26.85692388149907 27.72056118762033 22.815455570086886 56-57 22.77001662585929 26.926327860921294 27.423567763495555 22.880087749723863 58-59 22.660440138778277 26.578695068905866 27.411500428417312 23.349364363898548 60-61 22.225420542087488 26.890093982821224 27.84915904381471 23.03532643127658 62-63 22.456984719047046 26.888013066507604 27.894920087963403 22.76008212648195 64-65 22.083363468846066 26.935106346891008 27.660650018209314 23.32088016605361 66-67 22.05293973234457 26.966014564711127 27.601630293288444 23.37941540965586 68-69 22.815420013410446 26.454596635714434 27.740148201021476 22.989835149853644 70-71 22.498185423772505 26.665906406903567 27.271631214115644 23.564276955208285 72-73 22.399031476253814 26.759269782857775 27.394930685891744 23.446768054996667 74-75 22.398387373666317 27.11909083146501 27.24219364189858 23.24032815297009 76-77 22.415080480870948 26.621491162976408 27.53288505280983 23.43054330334282 78-79 22.558103389029352 26.89567379856331 27.365860203627523 23.180362608779816 80-81 22.75104672146762 26.87578990599991 27.071269236835583 23.301894135696887 82-83 22.61015927896885 26.962844638058737 27.27478051624152 23.152215566730884 84-85 22.449141041012055 27.11300121942196 27.193455418900758 23.244402320665223 86-87 22.62696612529953 27.054536936531015 26.836663116718206 23.481833821451247 88-89 22.369160480925647 27.194775040225945 26.64251935783496 23.793545121013448 90-91 22.218557938492182 27.099166228936966 26.956434127323593 23.725841705247262 92-93 22.40292298425529 26.70433909280206 27.144335789850338 23.748402133092313 94-95 22.04134995135729 26.469353557024505 27.336818401961228 24.15247808965698 96-97 22.27456888267814 26.002940034286315 27.489115714458933 24.233375368576613 98-99 21.912331873508357 26.47476407450089 26.985653726818036 24.627250325172717 100-101 22.01668521091026 26.12784928931939 27.448761819715134 24.406703680055216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 254.0 1 183.0 2 185.5 3 266.0 4 246.5 5 247.5 6 449.5 7 1026.0 8 1656.0 9 1911.0 10 1771.0 11 1587.0 12 1644.5 13 1824.0 14 2085.5 15 2399.0 16 2675.5 17 3034.5 18 3524.5 19 4064.0 20 4747.5 21 5749.0 22 7165.0 23 9006.5 24 11241.5 25 14356.5 26 19095.5 27 24897.0 28 29861.0 29 35369.0 30 41617.5 31 51163.0 32 66761.5 33 72343.0 34 70798.0 35 75798.5 36 84227.0 37 92476.0 38 98021.0 39 102723.5 40 109709.5 41 118967.0 42 124083.5 43 127336.0 44 132549.5 45 141155.5 46 145742.0 47 149149.5 48 152011.5 49 148698.5 50 145789.5 51 143359.0 52 140354.0 53 141222.0 54 143894.5 55 135527.5 56 122975.0 57 111849.0 58 103501.5 59 92115.5 60 73415.0 61 60598.5 62 52268.5 63 38511.0 64 25856.5 65 19470.0 66 14730.0 67 11442.0 68 8608.5 69 6428.5 70 4901.0 71 3286.0 72 2227.5 73 1826.0 74 1453.5 75 1066.5 76 795.0 77 537.5 78 326.5 79 203.5 80 125.5 81 73.5 82 43.5 83 20.5 84 10.5 85 9.0 86 6.0 87 5.0 88 3.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.375566514316286E-4 2 0.053013325005471554 3 0.0 4 0.0 5 0.044566006197260255 6 2.5597935782458503E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006719458142895357 26-27 0.0018046544726633246 28-29 1.0239174312983401E-4 30-31 0.001791855504772095 32-33 4.0956697251933605E-4 34-35 0.012299808143471312 36-37 0.02342211124094953 38-39 0.004543633601386384 40-41 0.0010239174312983402 42-43 0.0014334844038176762 44-45 0.008844086812839413 46-47 0.0 48-49 0.0 50-51 5.503556193228577E-4 52-53 0.0 54-55 0.0016382678900773442 56-57 0.0 58-59 0.023306920529928464 60-61 0.0 62-63 0.010111184634071109 64-65 0.015294766630018953 66-67 0.00701383440439363 68-69 0.01674105000172786 70-71 1.4078864680352177E-4 72-73 2.5597935782458503E-5 74-75 0.022692570071149464 76-77 0.04969839232164318 78-79 0.030691925003167746 80-81 0.026545059406409464 82-83 0.036745836815719184 84-85 0.015768328441994436 86-87 0.010367163991895694 88-89 0.08448598705000429 90-91 0.025508343007219898 92-93 0.024766002869528604 94-95 0.02678823979634282 96-97 0.03861448612783865 98-99 0.053794062046836545 100-101 0.015128380047432975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3906565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.42384606081505 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6555745845895 31.640240972080154 2 15.405234558186828 13.99530006203653 3 5.88767595545509 8.023226587696625 4 2.9257396840594736 5.3159339609094065 5 1.6879607247913881 3.833683405981291 6 1.0282784940032452 2.8025018411550087 7 0.6771137205190917 2.152997658456748 8 0.47889437779641875 1.7402579597131465 9 0.343024776654175 1.4023354184806238 >10 1.7260039068401032 13.874065411058023 >50 0.11558771506681981 3.615049354460062 >100 0.05978065025845615 4.997690035216109 >500 0.005129058746063849 1.601479497787051 >1k 0.003550886776121024 3.665440183105157 >5k 4.509062572852093E-4 1.3397976518640755 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8615 0.22052621676588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7338 0.1878376527716805 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6541 0.16743609795306105 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6423 0.16441554153073096 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6287 0.1609342222643166 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5947 0.1522309240982807 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5760 0.14744411010696096 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5420 0.13874081194092508 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4991 0.1277592974902504 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4698 0.12025910230599003 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4547 0.11639381400283882 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4438 0.11360363900255084 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4205 0.10763931996523801 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4130 0.10571947478155361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.679380734737551E-5 2 2.5597935782458503E-5 0.0 0.0 0.0 7.679380734737551E-5 3 2.5597935782458503E-5 0.0 0.0 0.0 7.679380734737551E-5 4 2.5597935782458503E-5 0.0 0.0 0.0 7.679380734737551E-5 5 2.5597935782458503E-5 0.0 0.0 0.0 7.679380734737551E-5 6 2.5597935782458503E-5 0.0 0.0 0.0 1.5358761469475102E-4 7 2.5597935782458503E-5 0.0 0.0 0.0 1.5358761469475102E-4 8 2.5597935782458503E-5 0.0 0.0 0.0 1.5358761469475102E-4 9 2.5597935782458503E-5 0.0 0.0 0.0 1.5358761469475102E-4 10-11 5.1195871564917006E-5 0.0 0.0 2.5597935782458503E-5 1.5358761469475102E-4 12-13 5.1195871564917006E-5 0.0 0.0 2.5597935782458503E-5 2.3038142204212652E-4 14-15 7.679380734737551E-5 0.0 0.0 5.1195871564917006E-5 2.431803899333558E-4 16-17 7.679380734737551E-5 0.0 0.0 5.1195871564917006E-5 2.5597935782458505E-4 18-19 1.4078864680352177E-4 0.0 0.0 5.1195871564917006E-5 2.5597935782458505E-4 20-21 2.0478348625966802E-4 0.0 0.0 6.399483945614626E-5 2.5597935782458505E-4 22-23 2.0478348625966802E-4 0.0 0.0 2.5597935782458505E-4 2.8157729360704355E-4 24-25 2.8157729360704355E-4 0.0 0.0 6.271494266702333E-4 2.8157729360704355E-4 26-27 2.8157729360704355E-4 0.0 0.0 0.0013438916285790714 2.8157729360704355E-4 28-29 2.943762614982728E-4 1.2798967891229251E-5 0.0 0.003327731651719606 2.8157729360704355E-4 30-31 3.3277316517196055E-4 2.5597935782458503E-5 0.0 0.0115958649094537 3.199741972807313E-4 32-33 3.3277316517196055E-4 2.5597935782458503E-5 0.0 0.028964064337851793 3.455721330631898E-4 34-35 3.455721330631898E-4 2.5597935782458503E-5 0.0 0.057211386473794756 3.8396903673687755E-4 36-37 3.5837110095441905E-4 2.5597935782458503E-5 0.0 0.10493873774018864 3.8396903673687755E-4 38-39 4.6076284408425305E-4 2.5597935782458503E-5 0.0 0.17871198866523402 3.8396903673687755E-4 40-41 4.6076284408425305E-4 2.5597935782458503E-5 0.0 0.25727205358159916 3.8396903673687755E-4 42-43 4.863607798667116E-4 2.5597935782458503E-5 0.0 0.3393646336359436 4.0956697251933605E-4 44-45 5.119587156491701E-4 2.5597935782458503E-5 0.0 0.4417691757336688 4.3516490830179455E-4 46-47 5.375566514316286E-4 2.5597935782458503E-5 0.0 0.5665719116410453 4.6076284408425305E-4 48-49 5.631545872140871E-4 2.5597935782458503E-5 0.0 0.6974925542004293 5.119587156491701E-4 50-51 5.631545872140871E-4 2.5597935782458503E-5 0.0 0.8411353708436952 5.631545872140871E-4 52-53 5.631545872140871E-4 2.5597935782458503E-5 0.0 0.9830119299179714 6.143504587790041E-4 54-55 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.1363947611264629 6.655463303439211E-4 56-57 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.3161690641266688 7.167422019088381E-4 58-59 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.5082687732061286 7.679380734737551E-4 60-61 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.7202068825169938 7.679380734737551E-4 62-63 5.631545872140871E-4 2.5597935782458503E-5 0.0 1.927729347905385 7.935360092562136E-4 64-65 5.631545872140871E-4 2.5597935782458503E-5 0.0 2.1455677814141065 8.447318808211306E-4 66-67 5.631545872140871E-4 2.5597935782458503E-5 0.0 2.4036077730691794 8.703298166035891E-4 68-69 5.631545872140871E-4 2.5597935782458503E-5 0.0 2.6856970253918724 8.703298166035891E-4 70-71 5.759535551053163E-4 2.5597935782458503E-5 0.0 2.98241549801424 9.087267202772768E-4 72-73 5.887525229965456E-4 2.5597935782458503E-5 0.0 3.2951454794685358 9.855205276246524E-4 74-75 6.015514908877748E-4 2.5597935782458503E-5 0.0 3.611382378125028 0.0010751133028632572 76-77 6.143504587790041E-4 2.5597935782458503E-5 0.0 3.9514381560270984 0.0011007112386457157 78-79 6.143504587790041E-4 2.5597935782458503E-5 0.0 4.336059940126428 0.0011263091744281742 80-81 7.039432340176088E-4 2.5597935782458503E-5 0.0 4.738945851406543 0.0011519071102106327 82-83 7.423401376912966E-4 2.5597935782458503E-5 0.0 5.157779276679129 0.0012542988533404667 84-85 7.423401376912966E-4 2.5597935782458503E-5 0.0 5.589270881196141 0.0012542988533404667 86-87 7.423401376912966E-4 2.5597935782458503E-5 0.0 6.036082850278953 0.001318293692796613 88-89 7.551391055825258E-4 2.5597935782458503E-5 0.0 6.51052779103893 0.0013822885322527592 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 48.174477 1 GTATCAA 11145 0.0 38.855427 1 ATCAACG 13835 0.0 30.837738 3 TCAACGC 14140 0.0 30.172565 4 TATCAAC 14345 0.0 29.88917 2 CAACGCA 14300 0.0 29.834972 5 AACGCAG 15010 0.0 28.472807 6 TAAGGTG 3085 0.0 26.334572 5 GTAAGGT 2945 0.0 25.811903 4 ACGCAGA 17030 0.0 24.928226 7 CGCAGAG 17350 0.0 24.468454 8 AGGTAAG 3005 0.0 24.360382 2 GGTAAGG 3225 0.0 23.128916 3 AAGGTAA 3085 0.0 22.342216 1 TACATGG 11645 0.0 22.083384 2 GTACATG 11920 0.0 21.653942 1 GCAGAGT 19355 0.0 21.516663 9 ACATGGG 11445 0.0 21.129406 3 GAGTACT 11865 0.0 20.511368 12-13 AGAGTAC 16590 0.0 20.408525 10-11 >>END_MODULE