FastQCFastQC Report
Thu 26 May 2016
SRR938566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916830
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91050.23245839109688193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68410.17465654623764626No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA67660.17274173247243307No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA65380.16692069862618494No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61680.15747428405113317No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57360.1464449567635052No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52790.1347773582208061No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49760.1270415106093448No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49150.1254841287469714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47890.12226724162141324No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA46640.1190758853460579No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46300.11820783643916126No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41080.10488073263327743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.043.7241971
GTATCAA110600.040.944121
ATCAACG140150.031.9477673
TATCAAC141850.031.6989252
TCAACGC142350.031.3538484
CAACGCA144200.030.88375
AACGCAG149400.030.02576
ACGCAGA171850.025.9037087
CGCAGAG173950.025.5090528
TAAGGTG26650.025.146465
GTACATG121250.023.7203621
TACATGG120750.023.382022
GTAAGGT26650.023.0078074
ACATGGG117200.022.8735853
GCAGAGT190150.022.2603029
GGTAAGG28450.022.2204113
AGAGTAC162900.020.71868310-11
AGGTAAG29650.020.5195582
AAGGTAA27950.019.9328861
GAGTACT115000.019.90935912-13
Produced by FastQC (version 0.11.5)