##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938566_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3916830 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125094783281376 31.0 31.0 34.0 28.0 34.0 2 31.228606296418278 31.0 31.0 34.0 28.0 34.0 3 31.300266286767616 31.0 31.0 34.0 27.0 34.0 4 34.93022929256567 37.0 35.0 37.0 32.0 37.0 5 34.956739761490795 37.0 35.0 37.0 32.0 37.0 6 34.98964392123222 37.0 35.0 37.0 32.0 37.0 7 34.913064136048796 37.0 35.0 37.0 32.0 37.0 8 34.86604345861321 37.0 35.0 37.0 32.0 37.0 9 36.3844430317374 39.0 37.0 39.0 32.0 39.0 10-11 36.23890390953909 39.0 35.0 39.0 32.0 39.0 12-13 36.10447632396607 39.0 35.0 39.0 31.0 39.0 14-15 37.1298867962102 39.0 36.0 41.0 31.0 41.0 16-17 36.997955872478514 39.0 36.0 41.0 31.0 41.0 18-19 36.979655103744605 39.0 36.0 41.0 31.0 41.0 20-21 37.050157653 39.0 36.0 41.0 31.0 41.0 22-23 36.98472170096736 39.0 36.0 41.0 31.0 41.0 24-25 36.877348519083036 39.0 36.0 41.0 30.0 41.0 26-27 36.700606102383816 39.0 36.0 41.0 30.0 41.0 28-29 36.52603444622309 39.0 36.0 41.0 30.0 41.0 30-31 36.30528986450778 39.0 35.5 40.5 29.5 41.0 32-33 36.02360786656557 38.5 35.0 40.0 29.0 41.0 34-35 35.7904403561043 38.0 35.0 40.0 27.5 41.0 36-37 35.511759126640676 38.0 34.5 40.0 26.5 41.0 38-39 35.253496705243776 38.0 34.0 40.0 26.0 41.0 40-41 35.1985534220275 38.0 34.0 40.0 25.5 41.0 42-43 35.02612801678909 38.0 34.0 40.0 25.0 41.0 44-45 34.764562669301455 38.0 33.5 40.0 24.0 41.0 46-47 34.436852633379544 38.0 33.0 40.0 24.0 41.0 48-49 34.09252648187437 38.0 33.0 40.0 23.0 41.0 50-51 33.33974834240955 37.5 32.0 39.5 21.5 40.5 52-53 33.388263212853246 37.5 32.5 39.5 22.0 40.5 54-55 33.518317619095036 37.5 33.0 40.0 20.5 41.0 56-57 33.23652813627346 37.0 32.0 40.0 19.5 41.0 58-59 32.91650543934763 37.0 32.0 40.0 18.0 41.0 60-61 32.480151934089555 36.0 31.0 39.0 15.0 41.0 62-63 31.97409818654371 36.0 31.0 39.0 11.5 40.5 64-65 31.49861393014249 35.0 30.0 39.0 8.0 40.0 66-67 30.980331160657983 35.0 29.5 38.0 7.0 40.0 68-69 30.447149863537604 35.0 29.0 38.0 2.0 40.0 70-71 29.83342881871309 34.0 28.5 37.0 2.0 39.0 72-73 29.310423224903815 34.0 28.0 36.5 2.0 39.0 74-75 28.741884891608777 34.0 26.5 36.0 2.0 39.0 76-77 28.040122624673522 33.5 26.0 35.5 2.0 38.0 78-79 27.299802518873683 33.0 25.0 35.0 2.0 37.0 80-81 26.69001373559741 32.0 24.5 35.0 2.0 36.5 82-83 26.058296122119163 32.0 22.5 35.0 2.0 36.0 84-85 25.444154328883307 31.5 20.0 34.5 2.0 36.0 86-87 24.817531396563034 31.0 18.5 34.0 2.0 35.0 88-89 24.054660018433275 31.0 11.5 34.0 2.0 35.0 90-91 23.265390124156525 30.0 4.5 34.0 2.0 35.0 92-93 22.46404783970711 29.5 2.0 34.0 2.0 35.0 94-95 21.54308484156831 29.0 2.0 34.0 2.0 35.0 96-97 20.34753793246069 28.0 2.0 34.0 2.0 35.0 98-99 18.89043167561523 26.0 2.0 33.5 2.0 35.0 100-101 15.371417957889415 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6955205740724857 1101 2 0.5434127864315705 1101 3 0.609318170556147 1101 4 0.5064315794291616 1101 5 0.47621395044523496 1101 6 0.4605673640014132 1101 7 0.39795117692510473 1101 8 0.40239132449012516 1101 9 0.4387316339084748 1101 10-11 0.608090788078151 1101 12-13 0.6667978683691231 1101 14-15 0.8234673050408787 1101 16-17 0.7433283813780918 1101 18-19 0.6727925409100095 1101 20-21 0.7329674276035902 1101 22-23 0.7331069773706247 1101 24-25 0.8785738347031966 1101 26-27 0.7985793153817795 1101 28-29 0.7235926925215779 1101 30-31 0.6200889252615553 1101 32-33 0.8216625993526208 1101 34-35 0.7677962907710452 1101 36-37 0.7874261795191373 1101 38-39 0.6414116266809273 1101 40-41 0.6946186171104571 1101 42-43 0.8613753202687207 1101 44-45 0.7267716386064649 1101 46-47 0.7673314595156384 1101 48-49 0.862156082798343 1101 50-51 0.7055627297024003 1101 52-53 0.8219868210113432 1101 54-55 0.8307147760747213 1101 56-57 0.7598356241454169 1101 58-59 0.8362846787830307 1101 60-61 0.7230051011173657 1101 62-63 0.644344402503318 1101 64-65 0.6245423633638296 1101 66-67 0.6361384972440227 1101 68-69 0.6125523137564457 1101 70-71 0.7419982653066519 1101 72-73 0.8627538156910362 1101 74-75 0.702361267929092 1101 76-77 0.8817517893032623 1101 78-79 0.9164118265586545 1101 80-81 0.95804921340223 1101 82-83 0.9943735558763791 1101 84-85 1.0242285235311535 1101 86-87 1.0566015156954016 1101 88-89 1.2307996599911988 1101 90-91 1.1041582612184584 1101 92-93 1.0487040253337447 1101 94-95 0.8616085803769273 1101 96-97 0.6275666120547676 1101 98-99 0.47383801107593726 1101 100-101 0.7192570817527741 1102 1 0.5757829141773207 1102 2 0.5269103079936954 1102 3 0.5595541779410134 1102 4 0.5872381319917395 1102 5 0.5649193581088525 1102 6 0.5313045884392622 1102 7 0.508991133737517 1102 8 0.5239137042447268 1102 9 0.5786096097700693 1102 10-11 0.5794474363583575 1102 12-13 0.6544231933788254 1102 14-15 0.6734416886198176 1102 16-17 0.680388638276078 1102 18-19 0.675914686906566 1102 20-21 0.595341032513204 1102 22-23 0.7683449087168768 1102 24-25 0.7175860934600635 1102 26-27 0.615305680902992 1102 28-29 0.538165968370464 1102 30-31 0.55503343611894 1102 32-33 0.6143466543207055 1102 34-35 0.5772687759395083 1102 36-37 0.5358211240212611 1102 38-39 0.7109060725254537 1102 40-41 0.7277618546155153 1102 42-43 0.860095765841578 1102 44-45 0.9539055650993475 1102 46-47 0.7708176950023926 1102 48-49 0.679708637954441 1102 50-51 0.8582266846244906 1102 52-53 0.8474299763604236 1102 54-55 0.8647253030479618 1102 56-57 0.8212550665067297 1102 58-59 0.934626359473576 1102 60-61 0.9139668503192837 1102 62-63 0.8300573841542693 1102 64-65 0.7605220134867636 1102 66-67 0.7920340084521378 1102 68-69 0.6855350478955593 1102 70-71 0.7339580285261853 1102 72-73 0.9002213583970828 1102 74-75 0.7756508313230697 1102 76-77 0.7554968139740836 1102 78-79 0.827003703467053 1102 80-81 0.9971399156241105 1102 82-83 0.8808297413139314 1102 84-85 0.7464967713548525 1102 86-87 0.6793237397860175 1102 88-89 0.8672673979323733 1102 90-91 0.9970673388656941 1102 92-93 0.9581696532505326 1102 94-95 0.6919091594871176 1102 96-97 0.9727260256854997 1102 98-99 1.1338776608407137 1102 100-101 0.7303194447442305 1103 1 0.5252865294636422 1103 2 0.627137535761463 1103 3 0.48627209183793596 1103 4 0.5160892313834253 1103 5 0.4853178313027584 1103 6 0.5719955911655887 1103 7 0.3932355099016789 1103 8 0.3514708186124693 1103 9 0.4280176043215391 1103 10-11 0.5282434170795725 1103 12-13 0.5467458971137091 1103 14-15 0.6966592667131692 1103 16-17 0.6682468613096972 1103 18-19 0.7412526846124976 1103 20-21 0.6369867620089309 1103 22-23 0.72167779513466 1103 24-25 0.7533463975710148 1103 26-27 0.7296636791764755 1103 28-29 0.7509194712767169 1103 30-31 0.6809060215925697 1103 32-33 0.7071433078828733 1103 34-35 0.8671588059253494 1103 36-37 0.890098743299383 1103 38-39 0.7425946195468001 1103 40-41 0.9364712790362759 1103 42-43 0.8898209826950136 1103 44-45 0.9314832636295876 1103 46-47 0.8768225537695358 1103 48-49 0.997882828910484 1103 50-51 1.1218349957731206 1103 52-53 1.1335766088037715 1103 54-55 1.1804986527060564 1103 56-57 1.1909486400941844 1103 58-59 1.210437809367697 1103 60-61 1.2278637304511903 1103 62-63 1.3986446772484804 1103 64-65 1.2401527817304796 1103 66-67 1.223323292637371 1103 68-69 1.3024836483575797 1103 70-71 1.354980360183312 1103 72-73 1.377508822292544 1103 74-75 1.3359253306919747 1103 76-77 1.3190735394348216 1103 78-79 1.3518687137221228 1103 80-81 1.3203970169545336 1103 82-83 1.3937955839423566 1103 84-85 1.372613816892116 1103 86-87 1.480262797093065 1103 88-89 1.451990494678757 1103 90-91 1.5854834523372787 1103 92-93 1.4916276979566199 1103 94-95 1.2671486534416267 1103 96-97 1.2847705779563192 1103 98-99 1.3984563682211153 1103 100-101 1.2024609502711083 1104 1 0.5243904247364846 1104 2 0.5588680800264036 1104 3 0.5296595699457392 1104 4 0.6291071884452748 1104 5 0.42794319970415273 1104 6 0.5623175211693763 1104 7 0.5345409358779349 1104 8 0.42395225217007493 1104 9 0.541973503371473 1104 10-11 0.5176893504862292 1104 12-13 0.6154126138512979 1104 14-15 0.6577338637072572 1104 16-17 0.716246511482268 1104 18-19 0.7628560335694488 1104 20-21 0.6821174335919125 1104 22-23 0.7332774458938758 1104 24-25 0.7427244805120736 1104 26-27 0.700612911821672 1104 28-29 0.7684308736671142 1104 30-31 0.623258598702229 1104 32-33 0.5319354203087059 1104 34-35 0.5965899449972696 1104 36-37 0.5922561565339564 1104 38-39 0.6440510811226332 1104 40-41 0.6445453840951885 1104 42-43 0.6075515830002658 1104 44-45 0.7626348712880926 1104 46-47 0.8497977287585172 1104 48-49 0.6890939705281554 1104 50-51 0.5226552916922387 1104 52-53 0.6286767862724929 1104 54-55 0.8412377951456378 1104 56-57 0.7062662493587126 1104 58-59 0.6865932723066308 1104 60-61 0.8868064565532165 1104 62-63 0.9041875353926052 1104 64-65 0.8816205779620248 1104 66-67 0.9782927603138027 1104 68-69 0.9630376879342393 1104 70-71 1.0214456882480327 1104 72-73 0.8912142303914798 1104 74-75 0.716704806721193 1104 76-77 0.8799207794762616 1104 78-79 1.0172178282967046 1104 80-81 0.9100350466850777 1104 82-83 1.0034541710517395 1104 84-85 0.9180250760091866 1104 86-87 1.0652934019757225 1104 88-89 1.032336098242613 1104 90-91 1.1335087683571885 1104 92-93 1.1833146033339368 1104 94-95 0.9787700101745962 1104 96-97 1.2209922981485626 1104 98-99 1.1293661302666713 1104 100-101 0.9307557677228662 1105 1 0.4736488640301033 1105 2 0.5342814038539565 1105 3 0.4631979390899268 1105 4 0.4489806022908027 1105 5 0.34147212565583374 1105 6 0.39086414191326213 1105 7 0.33028076769146963 1105 8 0.3708114012774786 1105 9 0.39175466915655477 1105 10-11 0.37538565348647523 1105 12-13 0.4392971096544258 1105 14-15 0.6339911963361331 1105 16-17 0.4738861379951942 1105 18-19 0.5766350279113368 1105 20-21 0.6115079764758207 1105 22-23 0.6630387185785551 1105 24-25 0.5926911375785906 1105 26-27 0.5969804458427319 1105 28-29 0.6126762242163863 1105 30-31 0.7381522306280246 1105 32-33 0.6589682415411815 1105 34-35 0.7916575884715442 1105 36-37 0.822712156716527 1105 38-39 0.7448862288064788 1105 40-41 0.6485633989564477 1105 42-43 0.7464447247119992 1105 44-45 0.5940549278316922 1105 46-47 0.561379115414347 1105 48-49 0.5707734621036664 1105 50-51 0.457007518728652 1105 52-53 0.5516125792074646 1105 54-55 0.6089798899744707 1105 56-57 0.6979612446400054 1105 58-59 0.7004800949655561 1105 60-61 0.7121639777281814 1105 62-63 0.6260859853975624 1105 64-65 0.7165490448399368 1105 66-67 0.6581306921362042 1105 68-69 0.722102098364445 1105 70-71 0.8278743964776503 1105 72-73 0.593117066902348 1105 74-75 0.6295342315819497 1105 76-77 0.6338437196744771 1105 78-79 0.7979039431061672 1105 80-81 0.7844723260057016 1105 82-83 0.9242176180683259 1105 84-85 0.7625389321393037 1105 86-87 0.7041639034239786 1105 88-89 0.9475835537947539 1105 90-91 0.7803741354125506 1105 92-93 0.7672611255150379 1105 94-95 1.0543229886799956 1105 96-97 1.2703264794145284 1105 98-99 1.2035887856669873 1105 100-101 0.9239541559383166 1106 1 0.280131928093482 1106 2 0.34563024880020876 1106 3 0.03183322211794959 1106 4 0.22881890169681185 1106 5 0.31363009736342917 1106 6 -0.012064697349558173 1106 7 0.24046138995805677 1106 8 0.24383477959310085 1106 9 0.326200984975209 1106 10-11 0.14798894294647624 1106 12-13 0.2441542614228851 1106 14-15 0.4914287305580203 1106 16-17 0.2328058005933471 1106 18-19 -0.0932432287996221 1106 20-21 0.19037295336088533 1106 22-23 0.16608056805075222 1106 24-25 0.36919295003917085 1106 26-27 0.07795231285040671 1106 28-29 0.11351207004287289 1106 30-31 0.16043541113246107 1106 32-33 0.5084897242240416 1106 34-35 0.2605502201088612 1106 36-37 0.12868565251286412 1106 38-39 0.16005943488637087 1106 40-41 0.3099825590559746 1106 42-43 -0.14723037700066044 1106 44-45 0.24042671443958596 1106 46-47 0.012892502670986516 1106 48-49 0.4515790193919784 1106 50-51 0.09189620661809528 1106 52-53 0.37785125566072253 1106 54-55 0.13623597020790612 1106 56-57 -0.1370503301560504 1106 58-59 -0.5792277627268234 1106 60-61 0.14317940616891178 1106 62-63 0.18634413107364978 1106 64-65 0.2317465787845805 1106 66-67 -0.28006821414953365 1106 68-69 -0.3433276592579375 1106 70-71 -0.3307622727534927 1106 72-73 0.20834471610978866 1106 74-75 0.23821948723245967 1106 76-77 0.10410453379156692 1106 78-79 0.20948262052976574 1106 80-81 0.08073231533193592 1106 82-83 0.1773982264728744 1106 84-85 0.08984404930812318 1106 86-87 0.41342051734561736 1106 88-89 0.23535755416903115 1106 90-91 0.2823389743704041 1106 92-93 0.24596270370728845 1106 94-95 0.01940889861837647 1106 96-97 0.015626696355315772 1106 98-99 -0.07523737753070492 1106 100-101 -0.4907972631871278 1107 1 0.48178696298879586 1107 2 0.46316098991021093 1107 3 0.4542116424126199 1107 4 0.37778644351580226 1107 5 0.4066022918909553 1107 6 0.4175309601224697 1107 7 0.4081970718014105 1107 8 0.38926293955765345 1107 9 0.40133779676207126 1107 10-11 0.38535019253105673 1107 12-13 0.4856597449722315 1107 14-15 0.5228771630383591 1107 16-17 0.5921904244994067 1107 18-19 0.464181164686579 1107 20-21 0.6621487371207166 1107 22-23 0.6578275493291414 1107 24-25 0.6977611343750567 1107 26-27 0.636172651833455 1107 28-29 0.7348441848096883 1107 30-31 0.7365836331331295 1107 32-33 0.7995687094904653 1107 34-35 0.9050411916387091 1107 36-37 0.8313803462095493 1107 38-39 0.8663073577843434 1107 40-41 0.7805696865623517 1107 42-43 0.9165394247053413 1107 44-45 0.9240286863017744 1107 46-47 0.9273768274743546 1107 48-49 0.9545772469315779 1107 50-51 0.8900306920506864 1107 52-53 0.7706234944997661 1107 54-55 0.8521587574018739 1107 56-57 0.8979009271830378 1107 58-59 0.9989111538567528 1107 60-61 0.9947580037096202 1107 62-63 0.9843883318047908 1107 64-65 0.9163145415629117 1107 66-67 0.9966100842884877 1107 68-69 0.8230421202922997 1107 70-71 0.8298497799772022 1107 72-73 0.7964315666692166 1107 74-75 0.8737711655308971 1107 76-77 0.934570912433422 1107 78-79 0.9925827645421741 1107 80-81 1.0009040224328558 1107 82-83 0.9431692467453914 1107 84-85 0.9186559469485331 1107 86-87 1.0403890561735771 1107 88-89 1.3060554604501888 1107 90-91 1.2192166746030395 1107 92-93 1.2292927136773777 1107 94-95 1.1258601005680866 1107 96-97 1.1201924435581532 1107 98-99 1.1282914803401276 1107 100-101 0.7296065254469699 1108 1 0.3382116618319948 1108 2 0.3333156550660341 1108 3 0.34904712366872914 1108 4 0.3297310402299658 1108 5 0.3891489937288597 1108 6 0.3194704062367748 1108 7 0.2702662762422108 1108 8 0.2697885214834628 1108 9 0.23374843692031533 1108 10-11 0.3258617452039161 1108 12-13 0.3850746000380312 1108 14-15 0.5622591094500464 1108 16-17 0.4200127079565448 1108 18-19 0.42646301532164443 1108 20-21 0.43102317162777126 1108 22-23 0.5050381929211198 1108 24-25 0.454477179352061 1108 26-27 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-0.8354879430930211 2316 48-49 -1.0027782103558636 2316 50-51 -1.1477755527270759 2316 52-53 -1.1025874981794104 2316 54-55 -0.9377031238313904 2316 56-57 -0.9310991126553816 2316 58-59 -0.9521380120000416 2316 60-61 -0.9156131960454772 2316 62-63 -0.9031022720932995 2316 64-65 -0.8061565572332334 2316 66-67 -0.9466544701030166 2316 68-69 -0.9624414919624158 2316 70-71 -1.2067759631928645 2316 72-73 -1.0399639785563046 2316 74-75 -0.949106547282291 2316 76-77 -0.9227802727072643 2316 78-79 -0.9497458489863249 2316 80-81 -0.8664188774269768 2316 82-83 -0.763311861978913 2316 84-85 -0.8440298914353654 2316 86-87 -0.9471440070971795 2316 88-89 -1.1615843645542938 2316 90-91 -1.0919221748842531 2316 92-93 -1.131514874074611 2316 94-95 -1.3257679018599617 2316 96-97 -1.2891315560518493 2316 98-99 -1.1870383713246504 2316 100-101 -0.8798391386123772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11535.0 3 3947.0 4 4023.0 5 4625.0 6 5590.0 7 6978.0 8 9104.0 9 11552.0 10 14935.0 11 18633.0 12 22798.0 13 26688.0 14 30541.0 15 35477.0 16 40130.0 17 45602.0 18 43694.0 19 42005.0 20 44037.0 21 47787.0 22 52854.0 23 59484.0 24 66527.0 25 74687.0 26 84696.0 27 96613.0 28 111864.0 29 128525.0 30 149853.0 31 176381.0 32 211094.0 33 254631.0 34 317204.0 35 397701.0 36 486919.0 37 502982.0 38 258664.0 39 16470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.521929760613936 18.19016390758926 14.524771841832848 23.763134489963956 2 15.549546083953969 19.41587220822027 40.21005734200679 24.824524365818963 3 19.410831645385322 23.101958871113997 29.625543740629013 27.861665742871665 4 13.135468854212778 16.02851459614327 36.56404072068657 34.27197582895738 5 13.942412208826314 36.98917754170664 34.307128925583946 14.761281323883107 6 33.16757622314952 35.653524124748806 16.910009856012294 14.268889796089375 7 29.24213579309179 32.26966964788765 20.145384964695797 18.342809594324766 8 26.81956014223496 34.66766509351441 19.427387560136104 19.085387204114525 9 26.332259657147294 16.797162041668784 19.770975189319394 37.099603111864525 10-11 25.170074977299922 26.153885448580333 27.281852581678773 21.394186992440968 12-13 26.258187581578905 23.30832761538726 27.394650914124302 23.038833888909537 14-15 22.601754088226443 25.05461971918396 25.59517903481347 26.748447157776123 16-17 22.529743396563724 28.12842302854626 26.568933043039657 22.772900531850368 18-19 22.7104278315594 26.580252817974763 28.619066257924906 22.09025309254093 20-21 22.84822677571795 25.92129359591705 28.920706227067676 22.30977340129732 22-23 22.62487121659023 25.828533742051572 28.886053358572987 22.66054168278521 24-25 22.580190246455345 26.020565686020408 28.904472734607257 22.49477133291699 26-27 22.045267348326234 26.409938059550036 28.595038556802972 22.949756035320757 28-29 22.315920168004983 26.99069147781346 28.324110972787054 22.3692773813945 30-31 22.422890361994522 26.353797898887372 28.24668698111747 22.976624758000636 32-33 21.950731074591012 26.369708004399577 28.304663541189328 23.374897379820084 34-35 22.863582270336323 26.590510792849877 28.069506836991405 22.4764000998224 36-37 23.084083837862927 25.905158398409412 28.16387499160288 22.846882772124783 38-39 22.401074929414026 26.416265215312762 28.018980579152313 23.163679276120895 40-41 23.040089523381717 26.204384566971882 28.18875653641861 22.566769373227793 42-43 22.695419503658478 26.307037867727896 27.913177644483252 23.08436498413038 44-45 22.611764357447818 26.497914814544366 28.235471488848457 22.65484933915936 46-47 23.091033440451824 26.792526107339697 27.41032885091958 22.706111601288896 48-49 22.713580387820674 26.894652736099655 27.35137045316984 23.040396422909833 50-51 22.894984009256262 27.038176711793447 27.395129484107493 22.671709794842798 52-53 23.292346805442023 26.54021199558427 27.514704325946244 22.65273687302747 54-55 22.584009018497877 26.886332966924943 27.696223184842182 22.833434829735 56-57 22.717929941314768 26.95538707127406 27.459741993336888 22.866940994074284 58-59 22.713015630972492 26.54617478183358 27.421802368558197 23.31900721863573 60-61 22.21746868827614 26.82799658715839 27.941319346964015 23.01321537760146 62-63 22.485936117093004 26.82499202449829 27.967170677523818 22.721901180884892 64-65 22.09764746869613 26.943368921033805 27.74478475226464 23.214198858005417 66-67 22.07672324101438 26.908704449565 27.707289984489396 23.307282324931222 68-69 22.837600085485263 26.405665109437347 27.870851376285934 22.885883428791452 70-71 22.553630886099157 26.581953290301197 27.373467805252442 23.4909480183472 72-73 22.472679060807284 26.696916582142592 27.563416039247773 23.266988317802355 74-75 22.51985663463192 27.093046401712357 27.402966566594532 22.984130397061193 76-77 22.609451681927514 26.599414249018217 27.53149624394205 23.25963782511222 78-79 22.75549724036912 26.912713859584485 27.361477815080093 22.970311084966298 80-81 23.019542800446207 26.877816709105872 27.091434038293073 23.011206452154852 82-83 22.85139811151062 26.939308039005542 27.282128926134696 22.927164923349135 84-85 22.76576755876176 27.076910153763116 27.258596427577046 22.89872585989808 86-87 22.999982382242525 27.046755741027134 26.857007386436315 23.09625449029402 88-89 22.77743917894529 27.141763180103208 26.725944234240757 23.354853406710742 90-91 22.703352111258763 27.095864472023766 26.939622972084937 23.261160444632534 92-93 22.971959442636305 26.589792731493244 27.220229651411476 23.218018174458972 94-95 22.652929724461785 26.450956220200723 27.416791871840978 23.479322183496514 96-97 23.005893475597773 25.944650034102246 27.443054488375036 23.60640200192495 98-99 22.711129088726427 26.330426526584272 26.990439943401157 23.968004441288144 100-101 23.259491931688718 25.703400252502984 27.341819458066947 23.695288357741358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 216.5 2 212.5 3 296.5 4 284.0 5 279.0 6 457.0 7 1031.0 8 1753.0 9 2076.0 10 1927.0 11 1748.0 12 1846.0 13 2017.0 14 2271.5 15 2557.0 16 2771.0 17 3163.0 18 3604.5 19 4129.0 20 4960.0 21 6029.5 22 7353.0 23 9145.0 24 11476.5 25 14603.0 26 19319.5 27 25231.5 28 30296.0 29 35395.0 30 41323.5 31 51039.0 32 66094.0 33 71909.0 34 71233.5 35 76120.0 36 84477.5 37 92892.5 38 98396.0 39 102772.0 40 109610.5 41 118839.0 42 123915.0 43 127279.0 44 132734.0 45 141270.5 46 145800.0 47 148674.5 48 151498.0 49 148483.5 50 145078.0 51 142260.5 52 139328.0 53 139503.0 54 141158.0 55 134319.5 56 122867.0 57 112296.5 58 104654.0 59 92468.5 60 73635.0 61 61329.5 62 53036.0 63 40153.5 64 28051.5 65 21214.0 66 16073.0 67 12322.5 68 9446.5 69 7106.5 70 5364.0 71 3794.5 72 2717.0 73 2152.0 74 1622.5 75 1200.5 76 930.0 77 654.0 78 401.5 79 240.0 80 154.5 81 100.0 82 68.0 83 45.5 84 30.0 85 26.5 86 24.0 87 14.0 88 8.5 89 8.0 90 7.5 91 7.0 92 3.0 93 2.5 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18818789684515283 2 0.04848308453519811 3 0.04646614736917354 4 0.0526701439684643 5 0.05833799271349535 6 0.06301013830061555 7 0.036560177490470615 8 0.03413474672120056 9 0.04138550817880786 10-11 0.04199824858367609 12-13 0.03894731198443639 14-15 0.03442835149853325 16-17 0.03204121700456747 18-19 0.03989195344194157 20-21 0.04885328186313932 22-23 0.04663209789549202 24-25 0.06616319830066661 26-27 0.051610613685046325 28-29 0.04360669214645517 30-31 0.05226165036521881 32-33 0.04871286218702369 34-35 0.04792140583073557 36-37 0.045878937814508164 38-39 0.02650102251055062 40-41 0.025058529474090013 42-43 0.021879938623836113 44-45 0.0038806892308320758 46-47 0.011501648016380593 48-49 0.010378290607455518 50-51 0.00882090874508212 52-53 0.009714488502181613 54-55 0.011105919838236533 56-57 0.008731550769372171 58-59 0.011208043239047904 60-61 0.01137399376536638 62-63 0.0037913312551221268 64-65 0.006676317328043342 66-67 0.003957281781440604 68-69 0.009331525749138973 70-71 0.023475616761513777 72-73 0.020067248259434286 74-75 0.0209735934416352 76-77 0.012510116599392877 78-79 0.004672145587120197 80-81 0.006306120000102123 82-83 0.0052976514170898405 84-85 0.004812565263235831 86-87 0.008348588016329532 88-89 0.030215761215064224 90-91 0.03617721473742797 92-93 0.02285011093154413 94-95 0.03209227870497316 96-97 0.03570489400867538 98-99 0.02167569182221337 100-101 0.12247148842303597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3916830.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.89166303189733 #Duplication Level Percentage of deduplicated Percentage of total 1 71.87564117107361 34.422439871666235 2 14.42875018400522 13.820336835676091 3 5.559296304105524 7.9873183587208185 4 2.6927212486449434 5.158355947157339 5 1.4580279857068759 3.491369249127486 6 0.9052720789939326 2.6012991215617536 7 0.6185506928536539 2.0736394945205676 8 0.423486793546297 1.6225189451982343 9 0.31483679255771563 1.3570251821295731 >10 1.5622536686788506 13.199935676977908 >50 0.10055809572219458 3.307604608154615 >100 0.05241380389147931 4.639710323846196 >500 0.004390044343079025 1.4217280149188603 >1k 0.0034263760014269684 3.706184630913631 >5k 3.747598751560745E-4 1.1905337394305877 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9105 0.23245839109688193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6841 0.17465654623764626 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6766 0.17274173247243307 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6538 0.16692069862618494 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6168 0.15747428405113317 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5736 0.1464449567635052 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5279 0.1347773582208061 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4976 0.1270415106093448 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4915 0.1254841287469714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4789 0.12226724162141324 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4664 0.1190758853460579 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4630 0.11820783643916126 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4108 0.10488073263327743 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5530850202842605E-5 0.0 4 0.0 0.0 0.0 2.5530850202842605E-5 0.0 5 0.0 0.0 0.0 2.5530850202842605E-5 0.0 6 2.5530850202842605E-5 0.0 0.0 2.5530850202842605E-5 2.5530850202842605E-5 7 2.5530850202842605E-5 0.0 0.0 2.5530850202842605E-5 2.5530850202842605E-5 8 2.5530850202842605E-5 0.0 0.0 2.5530850202842605E-5 2.5530850202842605E-5 9 2.5530850202842605E-5 0.0 0.0 2.5530850202842605E-5 2.5530850202842605E-5 10-11 2.5530850202842605E-5 0.0 0.0 2.5530850202842605E-5 2.5530850202842605E-5 12-13 2.5530850202842605E-5 0.0 0.0 7.659255060852782E-5 1.9148137652131954E-4 14-15 3.829627530426391E-5 0.0 0.0 7.659255060852782E-5 2.1701222672416214E-4 16-17 5.106170040568521E-5 0.0 0.0 1.0212340081137042E-4 2.5530850202842604E-4 18-19 5.106170040568521E-5 0.0 0.0 1.1488882591279172E-4 3.1913562753553256E-4 20-21 5.106170040568521E-5 0.0 0.0 1.5318510121705564E-4 3.574319028397965E-4 22-23 5.106170040568521E-5 0.0 0.0 2.5530850202842604E-4 3.574319028397965E-4 24-25 1.0212340081137042E-4 0.0 0.0 5.616787044625373E-4 3.829627530426391E-4 26-27 1.0212340081137042E-4 0.0 0.0 0.0011488882591279173 3.957281781440604E-4 28-29 1.6595052631847691E-4 0.0 0.0 0.003395603076978066 4.084936032454817E-4 30-31 2.0424680162274084E-4 0.0 0.0 0.011514413441482015 4.084936032454817E-4 32-33 2.0424680162274084E-4 0.0 0.0 0.030432773441788387 4.084936032454817E-4 34-35 2.1701222672416214E-4 0.0 0.0 0.05836352356369819 4.084936032454817E-4 36-37 2.5530850202842604E-4 0.0 0.0 0.1052253991110158 4.2125902834690295E-4 38-39 2.8083935223126863E-4 0.0 0.0 0.17768195198668313 4.7232072875258815E-4 40-41 2.8083935223126863E-4 0.0 0.0 0.2595210923118951 4.978515789554308E-4 42-43 3.1913562753553256E-4 0.0 0.0 0.34411756445901404 5.361478542596947E-4 44-45 3.574319028397965E-4 0.0 0.0 0.4525087889951823 5.872095546653799E-4 46-47 4.2125902834690295E-4 0.0 0.0 0.5779801523170524 6.127404048682226E-4 48-49 4.340244534483243E-4 0.0 0.0 0.710651215396124 6.255058299696438E-4 50-51 4.340244534483243E-4 0.0 0.0 0.8597258497305218 6.382712550710651E-4 52-53 4.340244534483243E-4 0.0 0.0 1.001715673133631 6.893329554767503E-4 54-55 4.595553036511669E-4 0.0 0.0 1.1552837371037292 6.893329554767503E-4 56-57 5.361478542596947E-4 0.0 0.0 1.3343698858515687 7.276292307810142E-4 58-59 5.361478542596947E-4 0.0 0.0 1.5277916069883042 7.659255060852782E-4 60-61 5.361478542596947E-4 0.0 0.0 1.737757319056482 7.659255060852782E-4 62-63 5.361478542596947E-4 0.0 0.0 1.9465996737157343 7.659255060852782E-4 64-65 5.616787044625373E-4 0.0 0.0 2.1614162473224523 7.659255060852782E-4 66-67 6.127404048682226E-4 0.0 0.0 2.4174013168812536 7.659255060852782E-4 68-69 6.382712550710651E-4 0.0 0.0 2.7004363222299665 7.914563562881207E-4 70-71 6.510366801724865E-4 0.0 0.0 2.993938976161845 7.914563562881207E-4 72-73 6.638021052739078E-4 0.0 0.0 3.3049174970575694 8.425180566938059E-4 74-75 6.638021052739078E-4 0.0 0.0 3.6145811791678475 9.063451822009125E-4 76-77 6.638021052739078E-4 0.0 0.0 3.949839538606475 9.446414575051764E-4 78-79 6.638021052739078E-4 0.0 0.0 4.328385454564023 9.446414575051764E-4 80-81 7.786909311866994E-4 2.5530850202842605E-5 0.0 4.723117929550172 9.446414575051764E-4 82-83 7.914563562881207E-4 2.5530850202842605E-5 0.0 5.1413898484233425 9.957031579108616E-4 84-85 8.169872064909634E-4 2.5530850202842605E-5 0.0 5.5718910445436745 0.0010084685830122828 86-87 8.42518056693806E-4 2.5530850202842605E-5 0.0 6.007919669732922 0.0011105919838236534 88-89 8.680489068966487E-4 2.5530850202842605E-5 0.0 6.463836827230184 0.0012382462348378663 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6360 0.0 43.724197 1 GTATCAA 11060 0.0 40.94412 1 ATCAACG 14015 0.0 31.947767 3 TATCAAC 14185 0.0 31.698925 2 TCAACGC 14235 0.0 31.353848 4 CAACGCA 14420 0.0 30.8837 5 AACGCAG 14940 0.0 30.0257 6 ACGCAGA 17185 0.0 25.903708 7 CGCAGAG 17395 0.0 25.509052 8 TAAGGTG 2665 0.0 25.14646 5 GTACATG 12125 0.0 23.720362 1 TACATGG 12075 0.0 23.38202 2 GTAAGGT 2665 0.0 23.007807 4 ACATGGG 11720 0.0 22.873585 3 GCAGAGT 19015 0.0 22.260302 9 GGTAAGG 2845 0.0 22.220411 3 AGAGTAC 16290 0.0 20.718683 10-11 AGGTAAG 2965 0.0 20.519558 2 AAGGTAA 2795 0.0 19.932886 1 GAGTACT 11500 0.0 19.909359 12-13 >>END_MODULE