FastQCFastQC Report
Thu 26 May 2016
SRR938566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3916830
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88270.22536081474049166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72630.18543056502324584No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65390.1669462294763878No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65340.16681857522537358No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62040.15839339465843552No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC59670.1523425831603618No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57570.1469811046177649No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53940.137713405994133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.12410546283601791No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA47730.12185874801816776No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45740.11677810882780207No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44090.11256551854433305No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA42110.10751041020417021No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41500.1059530283417968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65950.045.870911
GTATCAA111350.039.1291431
ATCAACG140650.030.5893883
TCAACGC141800.030.3405284
TATCAAC143800.030.0003812
CAACGCA145800.029.6385655
AACGCAG151000.028.6545056
TAAGGTG30750.026.7455675
GTAAGGT30900.026.6157344
AGGTAAG30450.025.3049872
GGTAAGG31750.025.3049163
ACGCAGA171100.025.0107237
CGCAGAG173700.024.5543258
AAGGTAA30650.023.5889261
GCAGAGT195850.021.4135489
GGTGTGC44450.021.3702558
GTACATG118350.021.341321
TACATGG115350.020.9471282
GTGTAGC24400.020.46891
AGAGTAC165850.020.3040310-11
Produced by FastQC (version 0.11.5)