##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3916830 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820548249477255 31.0 31.0 34.0 30.0 34.0 2 32.04980354010769 33.0 31.0 34.0 30.0 34.0 3 32.11968581735741 33.0 31.0 34.0 30.0 34.0 4 35.78056489559159 37.0 35.0 37.0 35.0 37.0 5 35.6453236418226 37.0 35.0 37.0 33.0 37.0 6 35.67237127983599 37.0 35.0 37.0 33.0 37.0 7 35.61115340721961 37.0 35.0 37.0 33.0 37.0 8 35.595217305831504 37.0 35.0 37.0 33.0 37.0 9 37.18968119627352 39.0 37.0 39.0 33.0 39.0 10-11 37.10412948736606 39.0 37.0 39.0 33.0 39.0 12-13 37.00163091071096 39.0 37.0 39.0 33.0 39.0 14-15 38.08249541082968 40.0 37.0 41.0 33.0 41.0 16-17 38.00996035058964 40.0 37.0 41.0 33.0 41.0 18-19 38.11088226959046 40.0 37.5 41.0 33.0 41.0 20-21 38.1480804630275 40.0 37.0 41.0 33.0 41.0 22-23 38.06337612303827 40.0 37.0 41.0 33.0 41.0 24-25 37.96025676376049 40.0 37.0 41.0 33.0 41.0 26-27 37.77342583160362 40.0 37.0 41.0 32.0 41.0 28-29 37.66066232131596 40.0 37.0 41.0 32.0 41.0 30-31 37.42663339996885 39.0 36.5 41.0 31.5 41.0 32-33 37.21830498132418 39.0 36.0 41.0 31.0 41.0 34-35 37.07294266026353 39.0 36.0 41.0 31.0 41.0 36-37 37.07074343282706 39.0 36.0 41.0 31.0 41.0 38-39 36.94443874255457 39.0 36.0 41.0 31.0 41.0 40-41 36.77894368660371 39.0 35.5 41.0 30.0 41.0 42-43 36.5695852258076 39.0 35.0 41.0 30.0 41.0 44-45 36.32627073424172 39.0 35.0 40.0 30.0 41.0 46-47 35.99090808128001 38.5 35.0 40.0 29.0 41.0 48-49 35.656413604879454 38.0 35.0 40.0 28.0 41.0 50-51 35.30081494473848 38.0 34.0 40.0 27.0 41.0 52-53 34.82245668052992 38.0 34.0 40.0 26.0 41.0 54-55 34.51465560159619 38.0 33.0 40.0 25.5 41.0 56-57 34.18321218944912 37.0 33.0 40.0 25.0 41.0 58-59 33.742080202612826 37.0 32.5 40.0 23.0 41.0 60-61 33.38181629021428 36.0 32.0 39.0 23.0 41.0 62-63 32.93642358744189 36.0 31.5 39.0 22.0 40.0 64-65 32.4465765682963 35.0 31.0 39.0 20.5 40.0 66-67 31.965485226573527 35.0 31.0 38.0 19.5 40.0 68-69 31.436302188249172 35.0 30.0 38.0 18.0 40.0 70-71 30.870005846564695 34.0 29.5 37.0 15.0 39.0 72-73 30.439890293936678 34.0 29.0 36.5 12.5 39.0 74-75 29.946314238810466 34.0 29.0 36.0 8.0 39.0 76-77 28.66340829190953 32.5 27.0 34.5 7.5 36.5 78-79 29.368473101972768 33.5 29.0 35.0 7.0 37.0 80-81 29.120433871268347 34.0 29.0 35.0 4.0 37.0 82-83 28.66836753190718 33.0 28.0 35.0 2.0 36.0 84-85 28.17645149266116 33.0 27.0 35.0 2.0 36.0 86-87 27.635585281975473 33.0 26.0 35.0 2.0 35.5 88-89 27.008776229757228 32.0 25.5 34.5 2.0 35.0 90-91 26.49978337073603 32.0 25.0 34.0 2.0 35.0 92-93 25.804390029692378 32.0 23.5 34.0 2.0 35.0 94-95 25.082205252717124 31.0 19.5 34.0 2.0 35.0 96-97 24.128223971936492 31.0 12.5 34.0 2.0 35.0 98-99 22.928130273716246 31.0 2.0 34.0 2.0 35.0 100-101 19.258447392406616 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34154890780946445 1101 2 0.34222322791336524 1101 3 0.30142499099503794 1101 4 0.17646423803282119 1101 5 0.2034343113247843 1101 6 0.18010948184211628 1101 7 0.2861656409460167 1101 8 0.2956920376572114 1101 9 0.28901566760042385 1101 10-11 0.38816419102764144 1101 12-13 0.32936477416594556 1101 14-15 0.45216189758682646 1101 16-17 0.4629199640231505 1101 18-19 0.4084604810031536 1101 20-21 0.4248381297805892 1101 22-23 0.4191002278251972 1101 24-25 0.471836395610417 1101 26-27 0.5151872651579765 1101 28-29 0.5563156770508613 1101 30-31 0.4508088832832797 1101 32-33 0.28985307471183575 1101 34-35 0.28711131014889446 1101 36-37 0.40237774135948 1101 38-39 0.5041058865695476 1101 40-41 0.5025989517129119 1101 42-43 0.520720404988495 1101 44-45 0.4241744662823237 1101 46-47 0.5088009398560658 1101 48-49 0.609481895260771 1101 50-51 0.5583219314240253 1101 52-53 0.5244414742223924 1101 54-55 0.5087129920817617 1101 56-57 0.5897247175912099 1101 58-59 0.671415843168262 1101 60-61 0.7560086501551666 1101 62-63 0.7047063255992043 1101 64-65 0.7372565194996739 1101 66-67 0.5381055865201958 1101 68-69 0.6508793981237311 1101 70-71 0.5122576991148549 1101 72-73 0.39892439669171864 1101 74-75 0.48851380853764326 1101 76-77 0.7065810596649271 1101 78-79 0.5871631077797836 1101 80-81 0.5705112902247613 1101 82-83 0.5953567432055671 1101 84-85 0.4701712245272738 1101 86-87 0.5454245393835926 1101 88-89 0.722308072610975 1101 90-91 0.771434369956495 1101 92-93 0.720622840913439 1101 94-95 0.8823688011514577 1101 96-97 0.9226901468450883 1101 98-99 0.914815109043797 1101 100-101 0.8765256651088684 1102 1 0.3517749481571819 1102 2 0.29865932640795734 1102 3 0.320344454102802 1102 4 0.2740958655993211 1102 5 0.22738822011110926 1102 6 0.25131439705022984 1102 7 0.32240093115844815 1102 8 0.3490084360539285 1102 9 0.42516286178337737 1102 10-11 0.4560113241068109 1102 12-13 0.3615225328841447 1102 14-15 0.4396949772746481 1102 16-17 0.43607072266667046 1102 18-19 0.512307879637028 1102 20-21 0.4171188735259719 1102 22-23 0.4266884974698044 1102 24-25 0.38963306019078914 1102 26-27 0.5195574746360947 1102 28-29 0.47070830581104417 1102 30-31 0.3212860562786446 1102 32-33 0.3331967579819235 1102 34-35 0.39810479488626527 1102 36-37 0.5039727293636531 1102 38-39 0.41250968871905513 1102 40-41 0.44509526211842143 1102 42-43 0.37969757383455516 1102 44-45 0.3724116066424088 1102 46-47 0.18614292060596682 1102 48-49 0.4225276637031783 1102 50-51 0.41964222753501446 1102 52-53 0.2864689407911172 1102 54-55 0.22766512540769668 1102 56-57 0.3633018487879056 1102 58-59 0.35927427105814047 1102 60-61 0.43042546710629637 1102 62-63 0.4601194706449263 1102 64-65 0.5494073947601592 1102 66-67 0.4050014397173527 1102 68-69 0.36626065039765265 1102 70-71 0.2756682777140256 1102 72-73 0.12855187321873274 1102 74-75 0.15149604912933867 1102 76-77 0.1754551207783237 1102 78-79 0.0859436525775088 1102 80-81 0.17258218023051697 1102 82-83 0.25182131894915116 1102 84-85 0.14345764319437038 1102 86-87 -0.04010517457292195 1102 88-89 0.2555107541894692 1102 90-91 0.10924237302435102 1102 92-93 0.20852459615148433 1102 94-95 0.16518085960668571 1102 96-97 0.019361060348938253 1102 98-99 0.03391526291703073 1102 100-101 -0.08102668039878225 1103 1 0.28069275586367937 1103 2 0.2768910296373903 1103 3 0.2425186351883255 1103 4 0.11628891584318524 1103 5 0.15523269020978603 1103 6 0.08363017496500902 1103 7 0.24345883784165778 1103 8 0.20209994694790367 1103 9 0.2945008496187711 1103 10-11 0.3565295267989086 1103 12-13 0.30042516054194124 1103 14-15 0.5021983651275619 1103 16-17 0.4816683645976596 1103 18-19 0.46621004872657323 1103 20-21 0.46301797781672605 1103 22-23 0.44593680745042974 1103 24-25 0.431065915310775 1103 26-27 0.5343293770280937 1103 28-29 0.5278768792031485 1103 30-31 0.5185974068505743 1103 32-33 0.6221273726978183 1103 34-35 0.5868821696632054 1103 36-37 0.6245251827860798 1103 38-39 0.6127279507577086 1103 40-41 0.637900264501738 1103 42-43 0.7137726498890302 1103 44-45 0.7933159854962781 1103 46-47 0.6981321567957579 1103 48-49 0.6586347934730554 1103 50-51 0.7161109640787018 1103 52-53 0.7666965880562984 1103 54-55 0.6921875296543334 1103 56-57 0.5662343002444388 1103 58-59 0.6857478894002114 1103 60-61 0.6930394871212613 1103 62-63 0.6738705959878786 1103 64-65 0.721207092146237 1103 66-67 0.5847587974758497 1103 68-69 0.5108420803985432 1103 70-71 0.2812573816387527 1103 72-73 0.22827681451994408 1103 74-75 0.5086388768956276 1103 76-77 0.464028381272648 1103 78-79 0.5739972298006073 1103 80-81 0.5197739534748855 1103 82-83 0.600244152604855 1103 84-85 0.4430543910200804 1103 86-87 0.6316123506719826 1103 88-89 0.5720401451244612 1103 90-91 0.5196823888007422 1103 92-93 0.49633889533786757 1103 94-95 0.531157603821427 1103 96-97 0.5352689370709847 1103 98-99 0.6440998787951742 1103 100-101 0.31036617852135606 1104 1 0.3115566127079923 1104 2 0.2703505998297757 1104 3 0.23862838744612702 1104 4 0.15563338617304368 1104 5 0.1476255646295357 1104 6 0.19686179538155812 1104 7 0.1442135218201983 1104 8 0.22304219771071843 1104 9 0.36698483046348684 1104 10-11 0.4065207695346089 1104 12-13 0.3326752819047343 1104 14-15 0.471727986950377 1104 16-17 0.46593990879938474 1104 18-19 0.4117594711523722 1104 20-21 0.4459711851560968 1104 22-23 0.4692569121300352 1104 24-25 0.44329347234125294 1104 26-27 0.4740702901409861 1104 28-29 0.538673517000305 1104 30-31 0.47009219737827834 1104 32-33 0.5625710282894687 1104 34-35 0.44859107202251636 1104 36-37 0.5607014377630293 1104 38-39 0.5058340578476646 1104 40-41 0.5177087995962921 1104 42-43 0.6353659177642896 1104 44-45 0.6821882698470105 1104 46-47 0.4989747144603527 1104 48-49 0.5943836059548246 1104 50-51 0.6736888305929867 1104 52-53 0.599266417107863 1104 54-55 0.6306165127178787 1104 56-57 0.6738101713838276 1104 58-59 0.6178010128411984 1104 60-61 0.5684404822151023 1104 62-63 0.4886768412257112 1104 64-65 0.4898950517144627 1104 66-67 0.520313861756641 1104 68-69 0.4317984493071627 1104 70-71 0.302064394613474 1104 72-73 0.13085118777731708 1104 74-75 0.08671121969501172 1104 76-77 0.18060733763985937 1104 78-79 0.12233921992192265 1104 80-81 0.19323370738070977 1104 82-83 0.2548146147730499 1104 84-85 0.27231375031253435 1104 86-87 0.351351023716024 1104 88-89 0.45162729781247535 1104 90-91 0.3359843933072817 1104 92-93 0.2160788034302037 1104 94-95 0.33029486855534174 1104 96-97 0.33289438690838224 1104 98-99 0.3763488191430362 1104 100-101 0.07068992790566853 1105 1 0.2378571026620726 1105 2 0.24749837727085833 1105 3 0.16115874995321633 1105 4 0.1733613263235938 1105 5 0.13418815300600073 1105 6 0.1702984472744049 1105 7 0.18008400420296766 1105 8 0.20448519783233365 1105 9 0.268845824012125 1105 10-11 0.24403333181157905 1105 12-13 0.36178502774738064 1105 14-15 0.4326090781200804 1105 16-17 0.3301458111269042 1105 18-19 0.3865500613533541 1105 20-21 0.39982599513243144 1105 22-23 0.30178861644284893 1105 24-25 0.38718649417152307 1105 26-27 0.32761851411883214 1105 28-29 0.263381959514831 1105 30-31 0.356621435599493 1105 32-33 0.4012720419088751 1105 34-35 0.41145782623635085 1105 36-37 0.3033480823965462 1105 38-39 0.27815179686601255 1105 40-41 0.3711298120139901 1105 42-43 0.3827998768017977 1105 44-45 0.4479920042555605 1105 46-47 0.16748847035224657 1105 48-49 0.14106656542651308 1105 50-51 0.12267453956760477 1105 52-53 0.236314106529953 1105 54-55 0.14739937533113334 1105 56-57 0.12494970242489245 1105 58-59 0.19762895694928773 1105 60-61 0.14179847453864625 1105 62-63 0.2172085634485228 1105 64-65 0.19412167475775988 1105 66-67 0.07352368059698478 1105 68-69 -0.0022456607145535656 1105 70-71 0.056032145923289534 1105 72-73 0.28208728116699433 1105 74-75 0.1479636039639054 1105 76-77 0.06593458369120953 1105 78-79 0.21961825745001917 1105 80-81 0.21538000146928127 1105 82-83 0.30892607217627344 1105 84-85 0.3130738435369764 1105 86-87 0.36066303268951216 1105 88-89 0.28056862464891097 1105 90-91 0.33476470778956724 1105 92-93 0.48658792120954786 1105 94-95 0.4732163883749223 1105 96-97 0.40226364866679987 1105 98-99 0.12866155636027443 1105 100-101 -0.1610707219731644 1106 1 0.11525256561926156 1106 2 0.12547625346905988 1106 3 0.2045710968644059 1106 4 0.06397985015588858 1106 5 -0.014087063761508034 1106 6 0.026141986528209316 1106 7 0.12657483100382194 1106 8 0.1461599391321755 1106 9 0.04718473681302271 1106 10-11 0.09503690821563282 1106 12-13 0.07230124978939045 1106 14-15 0.15955894608422483 1106 16-17 0.07454364449142759 1106 18-19 0.21469781674395705 1106 20-21 0.17508220012414455 1106 22-23 0.20325982846954815 1106 24-25 0.1700821338696059 1106 26-27 0.0703866577849297 1106 28-29 0.16503199174405836 1106 30-31 0.29954459142951606 1106 32-33 0.3188043924241981 1106 34-35 0.1231662176169408 1106 36-37 -0.20355527845291732 1106 38-39 -0.006560669951745979 1106 40-41 0.12288973549557625 1106 42-43 0.2453974135188588 1106 44-45 0.2149487685802356 1106 46-47 -0.029027308104772942 1106 48-49 0.010969565863170772 1106 50-51 -0.022634445107918566 1106 52-53 0.010766729572807776 1106 54-55 -0.20010383026358625 1106 56-57 0.029833053432312795 1106 58-59 -0.009279567506794706 1106 60-61 -0.4353988306824803 1106 62-63 -0.29096507928534265 1106 64-65 -0.5529554119977682 1106 66-67 -0.33962286186588386 1106 68-69 -0.9382255330452445 1106 70-71 -0.7269738008880964 1106 72-73 -0.4191009721477599 1106 74-75 -0.09874461414129243 1106 76-77 -0.1457384248547342 1106 78-79 -0.1918166707100042 1106 80-81 -0.9814261865414444 1106 82-83 -0.4871391902666282 1106 84-85 -0.345421190390347 1106 86-87 -0.3107101054664234 1106 88-89 -0.10736430391311202 1106 90-91 -0.28580814064991245 1106 92-93 -0.4238914278294672 1106 94-95 -0.17076021251640583 1106 96-97 -0.21502181907278484 1106 98-99 -0.2355532288822033 1106 100-101 -0.5022602575258119 1107 1 0.14514954135870894 1107 2 0.18872982608454691 1107 3 0.16359735143402787 1107 4 0.13380925084271666 1107 5 0.1180166251716912 1107 6 0.1208024495834863 1107 7 0.1154692588314461 1107 8 0.23386127955535585 1107 9 0.15242678843593183 1107 10-11 0.1994420261950296 1107 12-13 0.20530113901061497 1107 14-15 0.33379457253820277 1107 16-17 0.29372775881512325 1107 18-19 0.30603048540464073 1107 20-21 0.3430538407578041 1107 22-23 0.33555793415249724 1107 24-25 0.4007152345675138 1107 26-27 0.40736350502099583 1107 28-29 0.3952280701754276 1107 30-31 0.4070548384661876 1107 32-33 0.3539268029703351 1107 34-35 0.40809199583783595 1107 36-37 0.3727164618816303 1107 38-39 0.3295022523923308 1107 40-41 0.33763882157069247 1107 42-43 0.4503403624498503 1107 44-45 0.49915836202213626 1107 46-47 0.3297352929808639 1107 48-49 0.34058571740852983 1107 50-51 0.2434788815786817 1107 52-53 0.32158427693072866 1107 54-55 0.30905148157866336 1107 56-57 0.3109596735718938 1107 58-59 0.1461253289150477 1107 60-61 0.14575769972628194 1107 62-63 0.19515489412775366 1107 64-65 0.2819653811782601 1107 66-67 0.28902563708171414 1107 68-69 0.2847023499723669 1107 70-71 0.34916943606770445 1107 72-73 0.18130558595570534 1107 74-75 0.2080538792673714 1107 76-77 0.25472518431774915 1107 78-79 0.30131061410222415 1107 80-81 0.4152587586230574 1107 82-83 0.24523313719543083 1107 84-85 0.3387141812724437 1107 86-87 0.47206213337261715 1107 88-89 0.27643983921306514 1107 90-91 0.3617009985990087 1107 92-93 0.35712939858019155 1107 94-95 0.29372411228696294 1107 96-97 0.19210845676636623 1107 98-99 0.22308031493080094 1107 100-101 -0.34719157468543926 1108 1 0.17637983571549043 1108 2 0.16102505601590877 1108 3 0.11659193471984253 1108 4 0.09019599467027462 1108 5 0.10392176338558556 1108 6 0.09920954073316324 1108 7 0.15304662513352696 1108 8 0.12644404785562102 1108 9 0.214989285446741 1108 10-11 0.1691719201539854 1108 12-13 0.21834374550847002 1108 14-15 0.3306753631307586 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66-67 -0.4770513684946138 2316 68-69 -0.5043404585107005 2316 70-71 -0.5570832831668113 2316 72-73 -0.5399364188395808 2316 74-75 -0.3076153590743864 2316 76-77 -0.3670682943419621 2316 78-79 -0.37748129618164583 2316 80-81 -0.507633893599035 2316 82-83 -0.35760165495807783 2316 84-85 -0.4168028113878499 2316 86-87 -0.1464108126683321 2316 88-89 0.09135324830230829 2316 90-91 -0.034318068280271063 2316 92-93 -0.08689219070033616 2316 94-95 0.027856310854247113 2316 96-97 0.21669958873323836 2316 98-99 0.33371091000153186 2316 100-101 0.20116317704254882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 23.0 4 46.0 5 131.0 6 273.0 7 702.0 8 1608.0 9 2905.0 10 4969.0 11 7415.0 12 10578.0 13 14401.0 14 18979.0 15 24493.0 16 29773.0 17 36211.0 18 35163.0 19 31418.0 20 31003.0 21 32816.0 22 35966.0 23 40223.0 24 47466.0 25 55341.0 26 66819.0 27 80224.0 28 97138.0 29 116173.0 30 139186.0 31 166246.0 32 201369.0 33 249723.0 34 320221.0 35 423347.0 36 568641.0 37 661986.0 38 346716.0 39 17129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.160019076758424 18.998608050228654 15.071736985831318 23.769635887181604 2 15.903313477670041 20.042171998406097 39.11308532485977 24.941429199064093 3 19.36143149802185 23.285331465137794 30.054314857669233 27.29892217917112 4 13.548379684591877 16.694648478488983 36.16327489321722 33.59369694370192 5 13.905281973115482 37.12569999782798 34.34791441212959 14.621103616926948 6 33.2665446292027 35.96954680187805 16.608915883507834 14.154992685411417 7 29.242525205331866 32.333443115989205 20.026935046964 18.39709663171493 8 26.899967575820245 34.90611029837905 19.25245670606077 18.94146541973994 9 26.1900312242298 16.65188430439922 19.70764623432725 37.450438237043734 10-11 25.183630640083944 26.26655994771282 27.23154949282966 21.318259919373574 12-13 26.16996397597036 23.33743103479089 27.443123648460617 23.04948134077813 14-15 22.472254348542062 25.082847608908228 25.57229953814692 26.872598504402795 16-17 22.46873109121407 28.16288937737916 26.64316551905495 22.725214012351824 18-19 22.60768274344304 26.654373562293998 28.650911579006493 22.08703211525647 20-21 22.757357352757204 25.92771450382069 28.995526995044465 22.319401148377644 22-23 22.576177674292733 25.866721302686102 28.980948879578637 22.576152143442528 24-25 22.639499236945255 26.12870509194634 28.862068679395097 22.36972699171331 26-27 22.078611470808234 26.491772798063163 28.589480451817884 22.84013527931072 28-29 22.32250314667729 26.98821495954637 28.394683966370764 22.29459792740558 30-31 22.418167242387337 26.384691191601373 28.2719827002959 22.925158865715385 32-33 21.99417641306873 26.396307728443663 28.343035056410415 23.26648080207719 34-35 22.88568138750557 26.567535902463725 28.106432690452827 22.44035001957788 36-37 23.041032570364663 25.97341853834513 28.23994674261248 22.745602148677722 38-39 22.359796643473484 26.413535689009127 28.165471262818293 23.061196404699096 40-41 23.01068449228172 26.219849252325545 28.239018375835638 22.530447879557098 42-43 22.65453503791602 26.34230269806021 27.98486218646073 23.01830007756304 44-45 22.586224063847553 26.503333052493982 28.31819353916305 22.59224934449542 46-47 23.050055784907695 26.783649022296093 27.45951445429084 22.706780738505373 48-49 22.69661946012464 26.913537222703056 27.370258601981707 23.0195847151906 50-51 22.874850325390685 27.00395472869642 27.499431938582987 22.621763007329907 52-53 23.357664233576642 26.56144126755565 27.529047724818284 22.551846774049423 54-55 22.58856013664111 26.887419162945548 27.721397150246503 22.802623550166846 56-57 22.74853644401212 26.93038758383693 27.444400190970757 22.87667578118019 58-59 22.651927706844567 26.5879295246411 27.432426222225626 23.327716546288706 60-61 22.21143118286982 26.904614190557158 27.88710513348805 22.99684949308497 62-63 22.464033414776747 26.872815516629515 27.918495313812446 22.74465575478129 64-65 22.075109208211742 26.952739843189516 27.667246216966273 23.304904731632465 66-67 22.041284948250205 26.99975005278507 27.598500010340004 23.36046498862472 68-69 22.777350700227046 26.517379871992475 27.724066144795206 22.981203282985273 70-71 22.490060834940927 26.632647642429024 27.301722209910672 23.575569312719374 72-73 22.382028323925216 26.799184544644522 27.401904090808127 23.416883040622135 74-75 22.404674188055136 27.118064863678025 27.229851691291174 23.247409256975665 76-77 22.403844435423544 26.61011583346737 27.565454717207537 23.420585013901547 78-79 22.52683930627574 26.91061394035483 27.403499768945807 23.15904698442363 80-81 22.716978463350777 26.88932527247769 27.110897103583714 23.282799160587814 82-83 22.591163942740554 26.95117332283838 27.29409325742279 23.163569476998273 84-85 22.443184574493888 27.083551420481385 27.21508031139015 23.258183693634578 86-87 22.63983110456826 27.037119872302064 26.860557355945996 23.462491667183677 88-89 22.37318054303125 27.185135522928245 26.660935838898208 23.780748095142293 90-91 22.233590913988348 27.121402138840278 26.985445918530438 23.659561028640933 92-93 22.401759601919427 26.688989339498235 27.198359883527225 23.710891175055117 94-95 22.04151342303769 26.48759666459824 27.375355501177673 24.095534411186396 96-97 22.281232527324395 26.025842326575315 27.49848729712548 24.194437848974808 98-99 21.934808455926188 26.482954187151996 27.03724213502409 24.544995221897725 100-101 22.00133628538195 26.109520254655045 27.432312575379875 24.456830884583137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 280.0 1 209.5 2 186.5 3 256.0 4 254.0 5 256.5 6 489.0 7 1093.5 8 1736.0 9 2016.0 10 1835.5 11 1628.0 12 1734.0 13 1946.5 14 2133.0 15 2362.5 16 2695.5 17 3085.0 18 3507.5 19 4018.0 20 4757.0 21 5781.5 22 7036.5 23 8867.5 24 11336.5 25 14493.5 26 19096.0 27 24905.5 28 30118.5 29 35383.0 30 41282.5 31 51134.5 32 67110.5 33 72716.5 34 70914.5 35 75771.0 36 83990.5 37 92498.5 38 98660.5 39 103408.5 40 110383.0 41 119323.5 42 123980.5 43 127465.0 44 133458.5 45 142485.5 46 146353.0 47 149588.5 48 152642.5 49 148869.0 50 146036.0 51 143675.0 52 140735.0 53 142027.0 54 144554.0 55 136000.5 56 123560.5 57 112393.5 58 104049.0 59 91857.5 60 73022.0 61 60580.0 62 51850.0 63 38286.5 64 25773.0 65 19400.5 66 14625.5 67 11301.5 68 8587.5 69 6448.0 70 4862.0 71 3226.5 72 2245.5 73 1796.0 74 1430.0 75 1063.5 76 767.5 77 507.0 78 312.5 79 189.5 80 110.5 81 60.5 82 41.0 83 23.5 84 13.5 85 10.0 86 6.0 87 4.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.616787044625373E-4 2 0.048763923887429374 3 9.701723077080189E-4 4 0.0 5 0.08744316194473592 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9148137652131954E-4 26-27 0.008693254494067907 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010735722510295316 36-37 6.382712550710651E-5 38-39 0.004135997732860502 40-41 0.00559125619442253 42-43 4.084936032454817E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.659255060852782E-5 68-69 7.403946558824355E-4 70-71 5.106170040568521E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0010595302834179683 82-83 6.76567530375329E-4 84-85 0.005106170040568521 86-87 0.0022211839676473067 88-89 5.106170040568521E-5 90-91 0.0032679488259638534 92-93 8.935797570994912E-4 94-95 1.2765425101421302E-5 96-97 0.0 98-99 7.659255060852782E-5 100-101 5.106170040568521E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3916830.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.41059367076317 #Duplication Level Percentage of deduplicated Percentage of total 1 69.5232140155379 31.57090422345099 2 15.51162195858751 14.087839238718097 3 5.913071358284267 8.055482423918233 4 2.9753167591885967 5.404436015733012 5 1.6326549494070224 3.7069915256041357 6 1.0276515861847155 2.7999761169209623 7 0.6780852082921717 2.155457630753743 8 0.49122702010093217 1.7845528487922597 9 0.3529514952253531 1.4424963241650133 >10 1.7127788605291085 13.860994634969002 >50 0.11346739077570053 3.553794661364804 >100 0.05851750113577196 4.893208645635454 >500 0.0053953696182033484 1.6451450130113165 >1k 0.0035969130068882736 3.699221857981585 >5k 4.496141258610339E-4 1.3394988389814306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8827 0.22536081474049166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7263 0.18543056502324584 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6539 0.1669462294763878 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6534 0.16681857522537358 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6204 0.15839339465843552 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5967 0.1523425831603618 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5757 0.1469811046177649 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5394 0.137713405994133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4861 0.12410546283601791 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4773 0.12185874801816776 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4574 0.11677810882780207 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4409 0.11256551854433305 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4211 0.10751041020417021 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4150 0.1059530283417968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5530850202842605E-5 2 0.0 0.0 0.0 0.0 2.5530850202842605E-5 3 0.0 0.0 0.0 0.0 2.5530850202842605E-5 4 0.0 0.0 0.0 0.0 2.5530850202842605E-5 5 0.0 0.0 0.0 0.0 2.5530850202842605E-5 6 2.5530850202842605E-5 0.0 0.0 0.0 1.2765425101421302E-4 7 2.5530850202842605E-5 0.0 0.0 0.0 1.2765425101421302E-4 8 2.5530850202842605E-5 0.0 0.0 0.0 1.2765425101421302E-4 9 2.5530850202842605E-5 0.0 0.0 0.0 1.2765425101421302E-4 10-11 2.5530850202842605E-5 0.0 0.0 0.0 1.2765425101421302E-4 12-13 2.5530850202842605E-5 0.0 0.0 5.106170040568521E-5 2.2977765182558344E-4 14-15 5.106170040568521E-5 0.0 0.0 5.106170040568521E-5 2.5530850202842604E-4 16-17 5.106170040568521E-5 0.0 0.0 7.659255060852782E-5 2.5530850202842604E-4 18-19 6.382712550710652E-5 0.0 0.0 7.659255060852782E-5 2.8083935223126863E-4 20-21 7.659255060852782E-5 0.0 0.0 1.0212340081137042E-4 3.063702024341113E-4 22-23 8.935797570994912E-5 0.0 0.0 2.2977765182558344E-4 3.319010526369539E-4 24-25 1.0212340081137042E-4 0.0 0.0 5.361478542596947E-4 3.319010526369539E-4 26-27 1.0212340081137042E-4 0.0 0.0 0.0011105919838236534 4.2125902834690295E-4 28-29 1.2765425101421304E-4 0.0 0.0 0.0033700722267752236 4.340244534483243E-4 30-31 1.5318510121705564E-4 0.0 0.0 0.011629302267394805 4.467898785497456E-4 32-33 1.6595052631847694E-4 0.0 0.0 0.030726378219121074 4.8508615385400947E-4 34-35 1.7871595141989824E-4 0.0 0.0 0.05889967141795789 5.361478542596947E-4 36-37 1.7871595141989824E-4 0.0 0.0 0.10591473206649255 5.361478542596947E-4 38-39 2.2977765182558344E-4 0.0 0.0 0.17853723546847833 5.616787044625373E-4 40-41 2.2977765182558344E-4 0.0 0.0 0.2607465731216315 5.872095546653799E-4 42-43 2.4254307692700474E-4 0.0 0.0 0.3458919585481116 6.382712550710651E-4 44-45 2.5530850202842604E-4 0.0 0.0 0.45507463944056803 6.893329554767503E-4 46-47 3.7019732794121775E-4 0.0 0.0 0.5809544963656834 7.531600809838569E-4 48-49 3.829627530426391E-4 0.0 0.0 0.7140851147484062 7.659255060852782E-4 50-51 3.829627530426391E-4 0.0 0.0 0.8632491070585142 7.914563562881207E-4 52-53 3.829627530426391E-4 0.0 0.0 1.0056474240648687 8.935797570994912E-4 54-55 4.084936032454817E-4 0.0 0.0 1.159815463014734 8.935797570994912E-4 56-57 4.084936032454817E-4 0.0 0.0 1.3396037101431515 8.935797570994912E-4 58-59 4.084936032454817E-4 0.0 0.0 1.5339828381624936 8.935797570994912E-4 60-61 4.084936032454817E-4 0.0 0.0 1.744637883186148 8.935797570994912E-4 62-63 4.084936032454817E-4 0.0 0.0 1.9543738176024998 9.829377328094402E-4 64-65 4.084936032454817E-4 0.0 0.0 2.170237156067534 0.0010084685830122828 66-67 4.595553036511669E-4 0.0 0.0 2.4274349410109703 0.0010467648583165467 68-69 4.595553036511669E-4 0.0 0.0 2.7116443654690143 0.0010722957085193894 70-71 4.595553036511669E-4 0.0 0.0 3.0068065246640776 0.0010850611336208106 72-73 4.595553036511669E-4 0.0 0.0 3.3212444757622874 0.0011871845344321812 74-75 4.7232072875258815E-4 0.0 0.0 3.63552924175928 0.0012765425101421302 76-77 4.8508615385400947E-4 0.0 0.0 3.9766724621696623 0.0012765425101421302 78-79 4.8508615385400947E-4 0.0 0.0 4.36186916460505 0.0012765425101421302 80-81 5.361478542596947E-4 0.0 0.0 4.764069413275531 0.0012893079352435514 82-83 5.872095546653799E-4 0.0 0.0 5.191532438221726 0.0014680238866634499 84-85 5.999749797668012E-4 0.0 0.0 5.632220443572992 0.0015063201619677138 86-87 6.127404048682226E-4 0.0 0.0 6.078078446090334 0.0015446164372719777 88-89 6.127404048682226E-4 0.0 0.0 6.546186584559452 0.0015829127125762414 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6595 0.0 45.87091 1 GTATCAA 11135 0.0 39.129143 1 ATCAACG 14065 0.0 30.589388 3 TCAACGC 14180 0.0 30.340528 4 TATCAAC 14380 0.0 30.000381 2 CAACGCA 14580 0.0 29.638565 5 AACGCAG 15100 0.0 28.654505 6 TAAGGTG 3075 0.0 26.745567 5 GTAAGGT 3090 0.0 26.615734 4 AGGTAAG 3045 0.0 25.304987 2 GGTAAGG 3175 0.0 25.304916 3 ACGCAGA 17110 0.0 25.010723 7 CGCAGAG 17370 0.0 24.554325 8 AAGGTAA 3065 0.0 23.588926 1 GCAGAGT 19585 0.0 21.413548 9 GGTGTGC 4445 0.0 21.370255 8 GTACATG 11835 0.0 21.34132 1 TACATGG 11535 0.0 20.947128 2 GTGTAGC 2440 0.0 20.4689 1 AGAGTAC 16585 0.0 20.30403 10-11 >>END_MODULE