FastQCFastQC Report
Thu 26 May 2016
SRR938565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913457
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.23672165044869536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.18162969466637807No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA66170.16908324276975575No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65120.16640019297516237No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA63700.16277168753866467No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58640.14984194281424326No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC53230.13601784815829074No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA50880.1300129271894389No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48640.12428908762763971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.12290923344756312No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47530.12145272070192671No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44400.1134546770285198No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41030.1048433648306344No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39150.10003942805555292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64300.043.7435841
GTATCAA111300.040.305821
TATCAAC139150.031.96752
ATCAACG139450.031.9328083
TCAACGC142350.031.3490354
CAACGCA144000.030.9898285
AACGCAG150100.029.820066
ACGCAGA167350.026.5407497
CGCAGAG169300.026.0947578
TAAGGTG29250.025.6712655
GTAAGGT29750.024.1215954
GTACATG116400.023.9594141
AGGTAAG28400.023.260152
GGTAAGG30150.023.171073
TACATGG117150.022.79872
GCAGAGT186750.022.7661559
AAGGTAA28500.022.581991
ACATGGG117350.021.62593
AGAGTAC158550.021.07644510-11
GTGTAAG17550.020.6447561
Produced by FastQC (version 0.11.5)