FastQCFastQC Report
Thu 26 May 2016
SRR938565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913457
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89810.22949019243088653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71860.18362281737093317No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65860.16829110425897104No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65500.16737120147225332No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA62750.16034416629593734No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC61560.1573033765287315No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58700.1499952599453629No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53130.1357623196064247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.12950187008570682No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48420.12372692481353444No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA45310.11577998685050073No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA44880.11468121407747676No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41420.10583992618291194No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA40600.10374459205761044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67200.044.167041
GTATCAA111650.037.4892651
TATCAAC142400.029.4940032
ATCAACG142050.029.31993
TCAACGC143500.029.023644
CAACGCA145650.028.6278515
AACGCAG151000.027.6160776
TAAGGTG30000.027.575775
GTAAGGT31100.024.3072814
TAAGGTA14500.024.2640554
ACGCAGA173400.023.9390437
CGCAGAG175750.023.5378748
GGTAAGG31500.022.3383373
AGGTAAG29850.022.3083112
GTACATG120100.021.5842251
TACATGG117400.021.3108442
AAGGTAA29600.021.2111991
GCAGAGT194150.020.9157359
GTGTAAG16950.020.7656271
ACATGGG114100.020.584533
Produced by FastQC (version 0.11.5)