FastQCFastQC Report
Thu 26 May 2016
SRR938564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3925232
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87100.22189771203332695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68750.1751488829195319No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA67350.1715822147582614No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65430.16669078413709051No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61360.15632197026825423No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA57460.14638625181900075No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52010.13250172219119788No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49350.12572505268478398No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49310.12562314788017626No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA49050.12496076665022603No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA46000.1171905252988868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.1134455237295528No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG39500.10063099455013104No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39390.1003507563374598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.045.5531961
GTATCAA109150.040.3336871
TATCAAC139800.031.377622
ATCAACG139800.031.3100323
TCAACGC141600.030.8448934
CAACGCA145150.030.0905065
AACGCAG150000.029.1761116
ACGCAGA170150.025.5179627
CGCAGAG172350.025.1646678
GTACATG121550.023.3873621
TAAGGTG29100.023.0280515
GCAGAGT188350.022.4460289
TACATGG121450.022.305032
GTGTAAG18700.021.4253351
AGAGTAC161050.021.13224610-11
TAAGGTA16050.020.7278394
GTAAGGT29850.020.5388624
ATATGCG2550.020.50623994-95
AGGTAAG26950.020.4561882
ACATGGG123200.020.2911223
Produced by FastQC (version 0.11.5)