##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938564_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3925232 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1394506108174 31.0 31.0 34.0 28.0 34.0 2 31.319712822070134 31.0 31.0 34.0 28.0 34.0 3 31.381208295458713 31.0 31.0 34.0 28.0 34.0 4 35.048224155922505 37.0 35.0 37.0 32.0 37.0 5 35.047681767599975 37.0 35.0 37.0 32.0 37.0 6 35.01759055260938 37.0 35.0 37.0 32.0 37.0 7 34.93841867181354 37.0 35.0 37.0 32.0 37.0 8 34.88581184500687 37.0 35.0 37.0 32.0 37.0 9 36.383208686773166 39.0 37.0 39.0 32.0 39.0 10-11 36.222142028802374 39.0 35.0 39.0 32.0 39.0 12-13 36.05343327477204 38.0 35.0 39.0 30.5 39.0 14-15 37.23794975685513 39.0 36.5 41.0 31.5 41.0 16-17 37.1744847183555 39.0 36.0 41.0 31.0 41.0 18-19 37.15947949063903 39.5 36.0 41.0 31.0 41.0 20-21 37.07962472027131 39.0 36.0 41.0 31.0 41.0 22-23 36.94316157618199 39.0 36.0 41.0 30.5 41.0 24-25 36.810606863492396 39.0 36.0 41.0 30.0 41.0 26-27 36.607279136621734 39.0 36.0 41.0 30.0 41.0 28-29 36.38269343060487 39.0 35.5 40.5 29.5 41.0 30-31 36.106514213682146 39.0 35.0 40.0 29.0 41.0 32-33 35.81078213975633 38.0 35.0 40.0 27.5 41.0 34-35 35.52473267822131 38.0 34.5 40.0 27.0 41.0 36-37 35.31289195135472 38.0 34.0 40.0 26.0 41.0 38-39 35.07367258801518 38.0 34.0 40.0 25.0 41.0 40-41 34.81263476910409 38.0 34.0 40.0 24.5 41.0 42-43 34.41032237075414 38.0 33.0 40.0 24.0 41.0 44-45 33.97210394188165 38.0 33.0 40.0 23.0 41.0 46-47 33.51811013973187 38.0 32.5 40.0 20.5 41.0 48-49 33.05436837873532 37.0 31.5 40.0 18.5 41.0 50-51 32.31977358280989 36.5 30.5 39.5 15.0 40.5 52-53 32.31116020658142 36.5 31.0 39.0 14.5 40.0 54-55 32.30844482568164 37.0 31.0 39.0 11.0 40.5 56-57 31.90092687515031 36.0 30.5 39.0 8.0 40.5 58-59 31.4606754454259 36.0 30.0 39.0 7.0 40.0 60-61 30.925967815405563 35.0 29.0 39.0 2.0 40.0 62-63 30.223808936643742 35.0 28.0 38.0 2.0 40.0 64-65 29.580483395631138 34.0 27.5 38.0 2.0 40.0 66-67 28.909933986067575 34.0 26.0 37.5 2.0 40.0 68-69 28.096107058130578 33.5 25.5 37.0 2.0 39.0 70-71 27.278837403750913 33.0 23.5 36.0 2.0 39.0 72-73 26.400980757315743 32.0 21.0 35.5 2.0 38.5 74-75 25.506703552809107 31.5 18.5 35.0 2.0 37.5 76-77 24.59746748727209 31.0 11.5 35.0 2.0 37.0 78-79 23.63451956470344 30.0 6.5 34.0 2.0 36.0 80-81 22.7003737613471 29.5 2.0 34.0 2.0 36.0 82-83 21.713657434770735 29.0 2.0 34.0 2.0 35.0 84-85 20.754035048119448 27.5 2.0 33.5 2.0 35.0 86-87 19.792513155910278 26.0 2.0 33.0 2.0 35.0 88-89 18.70569230048058 25.0 2.0 32.0 2.0 34.5 90-91 17.561956083105407 21.5 2.0 32.0 2.0 34.0 92-93 16.41439448674626 15.5 2.0 31.0 2.0 34.0 94-95 15.141787287987054 3.5 2.0 31.0 2.0 34.0 96-97 13.707860962103641 2.0 2.0 30.5 2.0 34.0 98-99 11.94892671312167 2.0 2.0 29.5 2.0 34.0 100-101 8.411297727115238 2.0 2.0 12.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6005528723003373 1101 2 0.5698693921983811 1101 3 0.48665418761950363 1101 4 0.3586966622089136 1101 5 0.38693880788240875 1101 6 0.5095320223617321 1101 7 0.34013817197402574 1101 8 0.3942926011784422 1101 9 0.4425473002842466 1101 10-11 0.5200422001605816 1101 12-13 0.5363148759123675 1101 14-15 0.6615672279287068 1101 16-17 0.6467441730185968 1101 18-19 0.567249323839718 1101 20-21 0.6054904911772994 1101 22-23 0.5889206797412925 1101 24-25 0.6911473189801356 1101 26-27 0.7516302819702716 1101 28-29 0.8404348762993337 1101 30-31 0.9787573068720619 1101 32-33 0.8267388078874163 1101 34-35 0.840393239745957 1101 36-37 1.0661800752563408 1101 38-39 0.9789011943644184 1101 40-41 0.8973140624653908 1101 42-43 1.0781742543325592 1101 44-45 1.2585373729303129 1101 46-47 1.311191247297181 1101 48-49 1.1237544925708036 1101 50-51 1.2074082198339866 1101 52-53 1.2455813065198669 1101 54-55 1.3361373512406587 1101 56-57 1.5106304234497117 1101 58-59 1.3647001409373622 1101 60-61 1.476154621171876 1101 62-63 1.3691343648838519 1101 64-65 1.3692299279884246 1101 66-67 1.8202053124926287 1101 68-69 1.9206804155863217 1101 70-71 2.1378290482245603 1101 72-73 2.381580630063258 1101 74-75 2.344598861034644 1101 76-77 2.6699940409199954 1101 78-79 2.654788843233977 1101 80-81 2.8933891531465257 1101 82-83 3.138283293859974 1101 84-85 3.166847435284925 1101 86-87 3.4027743549751825 1101 88-89 3.790570845215669 1101 90-91 3.755755355849015 1101 92-93 3.6442909369675007 1101 94-95 3.6490386726640978 1101 96-97 3.4973415904027068 1101 98-99 3.253389715260324 1101 100-101 2.479300130134593 1102 1 0.6440788047070569 1102 2 0.5508764672020448 1102 3 0.5846572988726635 1102 4 0.3662910885378494 1102 5 0.45967851620306277 1102 6 0.4916151782157101 1102 7 0.4647893020293381 1102 8 0.5099099860574299 1102 9 0.5989356154214533 1102 10-11 0.6981404826660125 1102 12-13 0.6949077125318652 1102 14-15 0.8218783941442211 1102 16-17 0.8263151755665561 1102 18-19 0.807175902559564 1102 20-21 0.789713462218721 1102 22-23 0.6833700998087835 1102 24-25 0.7773391882260441 1102 26-27 0.752253359111613 1102 28-29 0.6916628431200209 1102 30-31 0.5699570973988557 1102 32-33 0.652974671278912 1102 34-35 0.6604140098360745 1102 36-37 0.8672783866469942 1102 38-39 0.9910217382551707 1102 40-41 0.9477035996432619 1102 42-43 1.1295495528024802 1102 44-45 1.2653098486754644 1102 46-47 1.3448063123268383 1102 48-49 1.389132355945769 1102 50-51 1.2097302845527196 1102 52-53 1.2004200788657542 1102 54-55 1.2910623958061151 1102 56-57 1.478649886803268 1102 58-59 1.4420703455546153 1102 60-61 1.6459793339152107 1102 62-63 1.6346081714876952 1102 64-65 1.7436377703845416 1102 66-67 1.97825069329906 1102 68-69 2.022932891641627 1102 70-71 1.7777871706240873 1102 72-73 1.879118783047037 1102 74-75 1.9412047883664236 1102 76-77 2.1613022745616988 1102 78-79 2.5590640854416336 1102 80-81 2.607686875551412 1102 82-83 2.8930584931658423 1102 84-85 3.0561425397007262 1102 86-87 3.0192152241663024 1102 88-89 3.2491320614690657 1102 90-91 3.06795798828923 1102 92-93 3.165668979959097 1102 94-95 3.2061592323181642 1102 96-97 2.9785638134107284 1102 98-99 2.8100318368769717 1102 100-101 2.114317687489857 1103 1 0.4966975349850351 1103 2 0.3745021093867962 1103 3 0.3782349285221933 1103 4 0.3095631802161307 1103 5 0.27442815363563255 1103 6 0.334107599088334 1103 7 0.3646659124682188 1103 8 0.37180891440220165 1103 9 0.4969857546972207 1103 10-11 0.5296752831071316 1103 12-13 0.5136660226257561 1103 14-15 0.554944745392639 1103 16-17 0.574137196983564 1103 18-19 0.5382084978345603 1103 20-21 0.5664379198154208 1103 22-23 0.5971351142833257 1103 24-25 0.5550409088427983 1103 26-27 0.6265651646934245 1103 28-29 0.6374904822208336 1103 30-31 0.6425881425019995 1103 32-33 0.7753336802049446 1103 34-35 0.6715519286386709 1103 36-37 0.8326725615979527 1103 38-39 0.798575447704124 1103 40-41 0.9844905000797937 1103 42-43 0.8842363049945803 1103 44-45 1.0173063625584078 1103 46-47 1.2242801692641265 1103 48-49 1.0133039607420429 1103 50-51 1.1399885982662497 1103 52-53 1.1901079209721601 1103 54-55 1.2733258301060033 1103 56-57 1.3735173154179066 1103 58-59 1.4893276224419232 1103 60-61 1.6292401403552752 1103 62-63 1.5900765249609847 1103 64-65 1.7776239127876785 1103 66-67 1.9621538252543012 1103 68-69 2.178017482666366 1103 70-71 2.0709923793688247 1103 72-73 2.2360257831926305 1103 74-75 2.340380354659356 1103 76-77 2.359998117684821 1103 78-79 2.4928620869168405 1103 80-81 2.613963253689743 1103 82-83 2.905416432286689 1103 84-85 2.819154588281144 1103 86-87 2.9896511174978784 1103 88-89 3.392117919931426 1103 90-91 3.2178768680986245 1103 92-93 3.3080584019184194 1103 94-95 3.235767981233277 1103 96-97 3.037142404276201 1103 98-99 2.7495144050850957 1103 100-101 1.9370326680604233 1104 1 0.4554327146871202 1104 2 0.40549904209752796 1104 3 0.46571389851851563 1104 4 0.36782286638290174 1104 5 0.3480030781524377 1104 6 0.4580116220858841 1104 7 0.42688617126560047 1104 8 0.4379256722332343 1104 9 0.6072756746089709 1104 10-11 0.452356212331658 1104 12-13 0.5479302216720754 1104 14-15 0.5895448999186641 1104 16-17 0.6368780256537647 1104 18-19 0.6845808081486169 1104 20-21 0.7474432457926596 1104 22-23 0.6468709886780886 1104 24-25 0.726733418526905 1104 26-27 0.7019597793638042 1104 28-29 0.7871813926879199 1104 30-31 0.7572024306893042 1104 32-33 0.7830628294949307 1104 34-35 0.9435644690781047 1104 36-37 1.0518439438565323 1104 38-39 0.9918922343120684 1104 40-41 0.9632080239462724 1104 42-43 1.00744863290452 1104 44-45 0.9931373896169404 1104 46-47 1.0002064449094874 1104 48-49 0.9763134488801839 1104 50-51 0.9612112349060098 1104 52-53 1.2018886573763865 1104 54-55 1.5248938346743373 1104 56-57 1.6336534212270237 1104 58-59 1.76768434825863 1104 60-61 1.9360822637617723 1104 62-63 1.9540224243631883 1104 64-65 1.9988221939666069 1104 66-67 2.144762527422987 1104 68-69 2.4115547274117652 1104 70-71 2.3083799601821156 1104 72-73 2.5307443577162196 1104 74-75 2.3893028690721714 1104 76-77 2.517405625012067 1104 78-79 2.6854212688131653 1104 80-81 2.9505882462894277 1104 82-83 2.9010568797377196 1104 84-85 2.9203950306362643 1104 86-87 2.9358764332383274 1104 88-89 3.1073883067635926 1104 90-91 2.997179822303618 1104 92-93 3.0773870455140937 1104 94-95 3.1623310959744764 1104 96-97 3.1962378199789523 1104 98-99 2.717749038850158 1104 100-101 1.8506893744677075 1105 1 0.5057724890149302 1105 2 0.44583897468073275 1105 3 0.3492429937724353 1105 4 0.34565508401571776 1105 5 0.35529548664125343 1105 6 0.4313759734761575 1105 7 0.3362969864391303 1105 8 0.37877233858338855 1105 9 0.409131693852828 1105 10-11 0.5363958394819548 1105 12-13 0.5110940634799874 1105 14-15 0.6148429739505374 1105 16-17 0.5999203273201985 1105 18-19 0.6333028958537525 1105 20-21 0.6097949209259923 1105 22-23 0.6222404358920102 1105 24-25 0.5718162652249106 1105 26-27 0.5440512826317772 1105 28-29 0.658925841880027 1105 30-31 0.7247125985982734 1105 32-33 0.7938615444611443 1105 34-35 0.8886289581505693 1105 36-37 0.9818653083345268 1105 38-39 1.0609906533406956 1105 40-41 1.0758462995955114 1105 42-43 1.2652292592793586 1105 44-45 1.1881241752916694 1105 46-47 1.3027521411540803 1105 48-49 1.1816719764720105 1105 50-51 1.2190599110996558 1105 52-53 1.3095611953181177 1105 54-55 1.4963167089087719 1105 56-57 1.5715299503697508 1105 58-59 1.6130159724337254 1105 60-61 1.746601183193956 1105 62-63 1.8348450358936788 1105 64-65 1.8117715997176056 1105 66-67 1.9699656482193255 1105 68-69 2.1402378842903396 1105 70-71 2.2400487632965813 1105 72-73 2.345198139228824 1105 74-75 2.3739865956701536 1105 76-77 2.5563589238457247 1105 78-79 2.6144152392827067 1105 80-81 2.690549631973642 1105 82-83 3.017583490381977 1105 84-85 3.2216300557197464 1105 86-87 3.3498025912721587 1105 88-89 3.2840557647072224 1105 90-91 3.0666077290595375 1105 92-93 3.180570827501466 1105 94-95 3.2832040327197944 1105 96-97 3.2861281489919154 1105 98-99 2.998502334518708 1105 100-101 2.3252732419129902 1106 1 0.2451918441823011 1106 2 0.21573265727717938 1106 3 0.12087647127359347 1106 4 0.20882891577237217 1106 5 0.2628100435162253 1106 6 -0.1158082709672712 1106 7 0.23034046093466998 1106 8 -0.05475284493766708 1106 9 0.19233649876778003 1106 10-11 0.22450387874858535 1106 12-13 0.36761263973355796 1106 14-15 0.38117667916554154 1106 16-17 -0.16630303601838392 1106 18-19 0.3151769757013341 1106 20-21 0.34774193033510414 1106 22-23 0.32314100084649056 1106 24-25 0.2997642425452227 1106 26-27 0.21897718648253317 1106 28-29 0.27963679812534536 1106 30-31 0.16160733570850283 1106 32-33 0.39158613806579723 1106 34-35 0.24469862737964831 1106 36-37 0.40449395137397204 1106 38-39 0.4909920287934497 1106 40-41 0.5724789874849066 1106 42-43 0.2614574621739223 1106 44-45 0.6822361167686992 1106 46-47 0.8209134155365305 1106 48-49 0.6794819500327449 1106 50-51 0.4404838612980697 1106 52-53 0.5247379409969 1106 54-55 0.30727937300793684 1106 56-57 0.870501519251917 1106 58-59 0.8486383648045468 1106 60-61 1.202041200718238 1106 62-63 1.1363096383623557 1106 64-65 1.0045623933538437 1106 66-67 1.3408429323623956 1106 68-69 1.5014003965802623 1106 70-71 1.3889770818310616 1106 72-73 1.6049813271207647 1106 74-75 1.8769495246713213 1106 76-77 1.9470831761343277 1106 78-79 2.2798735452343415 1106 80-81 2.1663838440684593 1106 82-83 2.4199176846468475 1106 84-85 2.5377837803948573 1106 86-87 2.2066088918646223 1106 88-89 2.2786963894684256 1106 90-91 2.3058510262744996 1106 92-93 2.327595111883962 1106 94-95 2.5654294133890794 1106 96-97 2.6057564710923824 1106 98-99 2.534272664474541 1106 100-101 1.8591364811551667 1107 1 0.17892506557473453 1107 2 0.17866591752513017 1107 3 0.17711328333237475 1107 4 0.10752432627246122 1107 5 0.12309091341730749 1107 6 0.17979376987265994 1107 7 0.23014136494226278 1107 8 0.21987627372816831 1107 9 0.27697715844816173 1107 10-11 0.25707871995363263 1107 12-13 0.22880919701114522 1107 14-15 0.24576673415689498 1107 16-17 0.29906900558477645 1107 18-19 0.3458070171857628 1107 20-21 0.40951787044209453 1107 22-23 0.39835746232671454 1107 24-25 0.46737569968118464 1107 26-27 0.43675206423026935 1107 28-29 0.4302847177745903 1107 30-31 0.48396620112732336 1107 32-33 0.5398853803060675 1107 34-35 0.5291577492911514 1107 36-37 0.6325338368735416 1107 38-39 0.6818331505769777 1107 40-41 0.6185287533881052 1107 42-43 0.8836210670410409 1107 44-45 0.5970043366539315 1107 46-47 0.7973398140075929 1107 48-49 0.6236586524480359 1107 50-51 0.8755119775840043 1107 52-53 0.8121643255909916 1107 54-55 0.953069196253864 1107 56-57 1.0979157846243197 1107 58-59 1.1065704031080692 1107 60-61 1.231475991673232 1107 62-63 1.246152516353714 1107 64-65 1.324561499060131 1107 66-67 1.4721598453838531 1107 68-69 1.4889754960511645 1107 70-71 1.5710153853558886 1107 72-73 1.5306148301386209 1107 74-75 1.5722746428375878 1107 76-77 1.8315823485271707 1107 78-79 2.2885769118646984 1107 80-81 2.267978211714148 1107 82-83 2.4091405781033153 1107 84-85 2.158648635654192 1107 86-87 2.155946382121833 1107 88-89 2.377191855938964 1107 90-91 2.379551717733147 1107 92-93 2.571657695483079 1107 94-95 2.499599952116238 1107 96-97 2.3693442425509996 1107 98-99 2.322335173292201 1107 100-101 1.8258231730625045 1108 1 0.26628657278119405 1108 2 0.236118671794582 1108 3 0.16349785962301056 1108 4 0.08651058195194139 1108 5 0.04602285613817969 1108 6 0.1634210829764413 1108 7 0.134965681557496 1108 8 0.16246408928478928 1108 9 0.26095819529174236 1108 10-11 0.266722636867577 1108 12-13 0.1688440834975964 1108 14-15 0.2702400213915013 1108 16-17 0.3918224089952034 1108 18-19 0.2910844102530916 1108 20-21 0.27135726834073637 1108 22-23 0.3861226600924397 1108 24-25 0.33866765915745134 1108 26-27 0.4284390811493708 1108 28-29 0.4412979999673752 1108 30-31 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0.30496369674230905 2316 90-91 0.05421003145754355 2316 92-93 0.15832867315469557 2316 94-95 -0.06888685553171214 2316 96-97 0.0426352188857404 2316 98-99 0.10065232026150817 2316 100-101 0.2951906060642404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11301.0 3 4122.0 4 4600.0 5 5318.0 6 6497.0 7 8400.0 8 11036.0 9 14276.0 10 18685.0 11 23618.0 12 29430.0 13 34759.0 14 40460.0 15 46305.0 16 53103.0 17 60323.0 18 60747.0 19 61717.0 20 66990.0 21 73981.0 22 81861.0 23 92094.0 24 102138.0 25 113176.0 26 125454.0 27 139767.0 28 155184.0 29 173005.0 30 193707.0 31 218840.0 32 248000.0 33 282214.0 34 322534.0 35 356803.0 36 357216.0 37 258345.0 38 68176.0 39 1050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50632610313581 18.210302328138948 14.491776193801007 23.79159537492423 2 15.529583313795964 19.41143761735163 40.20474482837356 24.854234240478846 3 19.4054084360593 23.129104684915657 29.63671069632786 27.828776182697183 4 13.075835759277677 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26.937697419206312 27.24103167536342 23.09201937496759 50-51 22.92481336821093 27.01805594641321 27.34970565817365 22.70742502720221 52-53 23.327792994917896 26.52925251547798 27.477479715469418 22.665474774134708 54-55 22.590905547099567 26.835155970932238 27.728185547751817 22.845752934216378 56-57 22.749773208788937 26.92789090479661 27.433207583801277 22.889128302613173 58-59 22.752347571092727 26.470525666119116 27.415821511349804 23.361305251438353 60-61 22.212149874998012 26.79957324161213 27.922380929632638 23.065895953757227 62-63 22.53182773016908 26.74181603136904 27.981853164835623 22.74450307362626 64-65 22.142835213470388 26.849829761533663 27.745156576458392 23.26217844853756 66-67 22.119096438092598 26.882148884305266 27.624909301396556 23.37384537620558 68-69 22.901984253918464 26.304998259225247 27.837364121797926 22.955653365058364 70-71 22.619002574354276 26.460850819819786 27.357333036748155 23.562813569077782 72-73 22.526522868134936 26.625217990286576 27.467215624035372 23.381043517543116 74-75 22.589030307338618 26.97422950046105 27.331576282773028 23.105163909427304 76-77 22.731046898296153 26.435960320040436 27.430507077190242 23.40248570447317 78-79 22.843276687662712 26.75235281917905 27.238738851211735 23.1656316419465 80-81 23.09420876523259 26.73107387889856 26.912052752169398 23.262664603699452 82-83 22.93588126773147 26.75036827697843 27.118575186278782 23.195175269011315 84-85 22.88965615552704 26.854789530279692 27.078629656631975 23.176924657561297 86-87 23.104549753180525 26.788027217400874 26.71042481075815 23.39699821866045 88-89 22.914219210142264 26.86174973420638 26.531726107399095 23.69230494825226 90-91 22.82572659680818 26.84154477617075 26.686392884800767 23.646335742220298 92-93 23.1253411007049 26.29999179574775 26.969835869092744 23.604831234454597 94-95 22.78830578220727 26.088656587336512 27.123069227634993 23.99996840282122 96-97 23.083364908730328 25.624574942495403 27.121936751188503 24.170123397585762 98-99 22.79550870641664 25.962628438149803 26.598720071016007 24.643142784417552 100-101 23.791233233395623 24.922100620287203 26.921632815891677 24.365033330425497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 195.5 2 187.5 3 263.5 4 267.5 5 266.5 6 424.0 7 905.0 8 1509.5 9 1779.0 10 1640.5 11 1515.5 12 1594.5 13 1782.5 14 2081.5 15 2357.5 16 2642.0 17 3026.5 18 3482.0 19 4031.0 20 4782.0 21 5871.5 22 7225.5 23 9002.5 24 11394.0 25 14678.5 26 19474.5 27 25194.5 28 30230.0 29 35267.0 30 41586.0 31 51688.0 32 65486.5 33 71135.5 34 71267.0 35 75945.5 36 83764.0 37 92122.0 38 97796.5 39 102390.0 40 109044.0 41 116890.5 42 122428.5 43 126978.0 44 132093.5 45 139635.0 46 144135.5 47 147114.5 48 149554.0 49 147434.0 50 144654.0 51 142206.5 52 139962.0 53 139529.5 54 140205.0 55 134324.5 56 124223.5 57 114114.0 58 106207.5 59 94382.5 60 76561.5 61 63676.5 62 54486.5 63 42372.0 64 31037.0 65 23760.5 66 17943.0 67 13708.0 68 10515.5 69 7824.0 70 5914.0 71 4283.0 72 3065.5 73 2362.5 74 1799.5 75 1353.0 76 1022.0 77 743.0 78 482.0 79 302.0 80 182.0 81 103.5 82 72.0 83 49.0 84 34.5 85 27.0 86 17.0 87 11.0 88 8.5 89 7.0 90 2.5 91 2.0 92 5.0 93 4.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3883082579577462 2 0.028737154899379197 3 0.034698585968931266 4 0.04264716072833402 5 0.06149954958076364 6 0.053984070240943725 7 0.03885120675669616 8 0.04045620742926787 9 0.037271682285276386 10-11 0.03761561100082747 12-13 0.03036763177310284 14-15 0.028673464396499367 16-17 0.02277572382982713 18-19 0.027616202048694194 20-21 0.03380691892861364 22-23 0.032902513787720064 24-25 0.041207755363249866 26-27 0.0358450150207682 28-29 0.03131025121572432 30-31 0.03406168094013297 32-33 0.028864535905138856 34-35 0.030316679370798974 36-37 0.03245668026756125 38-39 0.015897149518805512 40-41 0.01366798191801147 42-43 0.014355839349113633 44-45 0.011693576328736749 46-47 0.020113460809450243 48-49 0.01741298348734546 50-51 0.011680838228160781 52-53 0.009719170739462024 54-55 0.008509051184745257 56-57 0.009337027722183045 58-59 0.0041908350894928 60-61 0.007502741239243948 62-63 0.011986552641983964 64-65 0.0075536936415478125 66-67 0.0036685729658781953 68-69 0.006509169394318604 70-71 0.014228458343353971 72-73 0.011680838228160781 74-75 0.012050243144863795 76-77 0.005808573862640476 78-79 0.00932428962160708 80-81 0.011833695435072374 82-83 0.01366798191801147 84-85 0.00847083688301736 86-87 0.0024457153105854634 88-89 0.016928935665458753 90-91 0.02580739176690703 92-93 0.011158576104546177 94-95 0.02117072315725542 96-97 0.019438341478924048 98-99 0.012305005156383112 100-101 0.12203100351775384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3925232.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.1117781314084 #Duplication Level Percentage of deduplicated Percentage of total 1 72.21622776898407 34.744511279086154 2 14.152585220566777 13.618120802355168 3 5.4965860804639615 7.933515899504097 4 2.646643302911056 5.093388615305385 5 1.4856035444394047 3.573751406065128 6 0.944494424266155 2.7264783711987337 7 0.6160740211966682 2.074829163423709 8 0.4283963647005954 1.6488728680621572 9 0.307529122377742 1.3316195614306199 >10 1.5464242126255308 13.099781935670052 >50 0.10015442735813956 3.322753140010906 >100 0.0510861530994571 4.532851880369276 >500 0.004470194783102362 1.4544810474298648 >1k 0.0033526460046271987 3.668667728755818 >5k 3.7251622273635533E-4 1.176376301333083 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8710 0.22189771203332695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6875 0.1751488829195319 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6735 0.1715822147582614 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6543 0.16669078413709051 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6136 0.15632197026825423 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5746 0.14638625181900075 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5201 0.13250172219119788 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4935 0.12572505268478398 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4931 0.12562314788017626 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4905 0.12496076665022603 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4600 0.1171905252988868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4453 0.1134455237295528 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3950 0.10063099455013104 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3939 0.1003507563374598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.095240230386382E-5 7 0.0 0.0 0.0 0.0 5.095240230386382E-5 8 0.0 0.0 0.0 0.0 5.095240230386382E-5 9 0.0 0.0 0.0 0.0 5.095240230386382E-5 10-11 0.0 0.0 0.0 3.8214301727897865E-5 5.095240230386382E-5 12-13 0.0 0.0 0.0 5.095240230386382E-5 2.038096092154553E-4 14-15 2.547620115193191E-5 0.0 0.0 6.369050287982977E-5 2.038096092154553E-4 16-17 7.642860345579573E-5 0.0 0.0 7.642860345579573E-5 2.292858103673872E-4 18-19 1.401191063356255E-4 0.0 0.0 7.642860345579573E-5 2.292858103673872E-4 20-21 1.5285720691159146E-4 1.2738100575965956E-5 0.0 1.146429051836936E-4 2.4202391094335315E-4 22-23 1.5285720691159146E-4 2.547620115193191E-5 0.0 2.1654770979142123E-4 2.547620115193191E-4 24-25 1.5285720691159146E-4 2.547620115193191E-5 0.0 4.840478218867063E-4 2.547620115193191E-4 26-27 1.5285720691159146E-4 2.547620115193191E-5 0.0 9.680956437734126E-4 3.439287155510808E-4 28-29 2.292858103673872E-4 2.547620115193191E-5 0.0 0.002904286931320238 3.821430172789787E-4 30-31 3.439287155510808E-4 2.547620115193191E-5 0.0 0.011094885601666347 3.821430172789787E-4 32-33 3.5666681612704675E-4 2.547620115193191E-5 0.0 0.02947596473278522 4.3309541958284247E-4 34-35 3.694049167030127E-4 2.547620115193191E-5 0.0 0.05695204767514378 4.840478218867063E-4 36-37 3.821430172789787E-4 2.547620115193191E-5 0.0 0.10452885332637663 4.840478218867063E-4 38-39 4.585716207347744E-4 2.547620115193191E-5 0.0 0.17967090862399981 5.222621236146041E-4 40-41 4.585716207347744E-4 2.547620115193191E-5 0.0 0.2617552287355244 5.350002241905702E-4 42-43 4.7130972131074035E-4 2.547620115193191E-5 0.0 0.34480764449082246 5.350002241905702E-4 44-45 4.840478218867063E-4 2.547620115193191E-5 0.0 0.45108161759610643 5.604764253425021E-4 46-47 4.840478218867063E-4 2.547620115193191E-5 0.0 0.5789721473788045 5.85952626494434E-4 48-49 4.840478218867063E-4 3.8214301727897865E-5 0.0 0.7125693462195355 6.114288276463658E-4 50-51 4.840478218867063E-4 5.095240230386382E-5 0.0 0.8569684543486856 6.623812299502297E-4 52-53 4.840478218867063E-4 5.095240230386382E-5 0.0 0.9951004169944604 7.642860345579574E-4 54-55 4.840478218867063E-4 5.095240230386382E-5 0.0 1.1497027436849592 7.642860345579574E-4 56-57 5.350002241905702E-4 5.095240230386382E-5 0.0 1.3319594867258802 7.642860345579574E-4 58-59 5.350002241905702E-4 5.095240230386382E-5 0.0 1.5182796838505341 8.152384368618212E-4 60-61 5.350002241905702E-4 5.095240230386382E-5 0.0 1.726407509161242 8.40714638013753E-4 62-63 5.604764253425021E-4 5.095240230386382E-5 0.0 1.9348920012880768 8.40714638013753E-4 64-65 5.604764253425021E-4 5.095240230386382E-5 0.0 2.1504206630334206 8.40714638013753E-4 66-67 5.85952626494434E-4 5.095240230386382E-5 0.0 2.405106245949284 8.40714638013753E-4 68-69 5.85952626494434E-4 5.095240230386382E-5 0.0 2.6849113632009525 8.40714638013753E-4 70-71 5.986907270703999E-4 5.095240230386382E-5 0.0 2.9771106523130353 8.40714638013753E-4 72-73 6.241669282223318E-4 6.369050287982977E-5 0.0 3.2823028040126037 9.298813420455148E-4 74-75 6.369050287982977E-4 7.642860345579573E-5 0.0 3.5857625740338404 0.0010317861466532423 76-77 6.369050287982977E-4 1.0190480460772765E-4 0.0 3.9105968768215487 0.0010445242472292083 78-79 6.369050287982977E-4 1.146429051836936E-4 0.0 4.281504889392525 0.0010445242472292083 80-81 6.878574311021616E-4 1.2738100575965955E-4 0.0 4.666743265111464 0.0010445242472292083 82-83 7.260717328300594E-4 1.2738100575965955E-4 0.0 5.0659425990616604 0.0010954766495330723 84-85 7.642860345579573E-4 1.2738100575965955E-4 0.0 5.469740387319781 0.0010954766495330723 86-87 7.897622357098893E-4 1.2738100575965955E-4 0.0 5.879754368658974 0.001146429051836936 88-89 8.025003362858552E-4 1.2738100575965955E-4 0.0 6.303372641413297 0.0011973814541407998 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6230 0.0 45.553196 1 GTATCAA 10915 0.0 40.333687 1 TATCAAC 13980 0.0 31.37762 2 ATCAACG 13980 0.0 31.310032 3 TCAACGC 14160 0.0 30.844893 4 CAACGCA 14515 0.0 30.090506 5 AACGCAG 15000 0.0 29.176111 6 ACGCAGA 17015 0.0 25.517962 7 CGCAGAG 17235 0.0 25.164667 8 GTACATG 12155 0.0 23.387362 1 TAAGGTG 2910 0.0 23.028051 5 GCAGAGT 18835 0.0 22.446028 9 TACATGG 12145 0.0 22.30503 2 GTGTAAG 1870 0.0 21.425335 1 AGAGTAC 16105 0.0 21.132246 10-11 TAAGGTA 1605 0.0 20.727839 4 GTAAGGT 2985 0.0 20.538862 4 ATATGCG 255 0.0 20.506239 94-95 AGGTAAG 2695 0.0 20.456188 2 ACATGGG 12320 0.0 20.291122 3 >>END_MODULE