FastQCFastQC Report
Thu 26 May 2016
SRR938564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3925232
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87200.22215247404484628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72210.18396364851810035No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65620.1671748319589772No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA65240.1662067363152038No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA63280.16121340088942512No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC60680.15458958858992283No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA58280.14847530031345918No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53500.13629767616283572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.12404362340875648No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48180.12274433715000796No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA46530.11854076395993918No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA43370.1104902843959287No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA41930.10682171143005051No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG41350.10534409176323845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.045.331271
GTATCAA113400.037.2894481
ATCAACG143850.028.9908433
TATCAAC146600.028.6771032
TCAACGC147400.028.356714
CAACGCA148400.028.1976365
AACGCAG155800.026.8579946
ACGCAGA177250.023.5541677
TAAGGTG28350.023.4555515
CGCAGAG179950.023.0687878
GAGTACT117650.021.21514112-13
GTACATG119600.021.0151981
AGGTAAG27650.020.788172
GTAAGGT28750.020.4858744
TACATGG120200.020.4715752
AAGGTAA27500.020.3872341
GTGTAGC22900.020.3329121
GCAGAGT203250.020.2606629
GTACTTT122450.020.17017614-15
AGAGTAC169550.020.12769710-11
Produced by FastQC (version 0.11.5)