##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938564_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3925232 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847875233871527 31.0 31.0 34.0 30.0 34.0 2 32.1039500340362 33.0 31.0 34.0 30.0 34.0 3 32.1643599664937 34.0 31.0 34.0 30.0 34.0 4 35.8098306036433 37.0 35.0 37.0 35.0 37.0 5 35.70090634133218 37.0 35.0 37.0 33.0 37.0 6 35.73085157769018 37.0 35.0 37.0 33.0 37.0 7 35.65383370970174 37.0 35.0 37.0 33.0 37.0 8 35.622435310830035 37.0 35.0 37.0 33.0 37.0 9 37.182275850191786 39.0 37.0 39.0 33.0 39.0 10-11 37.112123181508764 39.0 37.0 39.0 33.0 39.0 12-13 37.000812563435744 39.0 37.0 39.0 33.0 39.0 14-15 38.04338635270476 40.0 37.0 41.0 33.0 41.0 16-17 38.125928225388975 40.0 37.0 41.0 33.0 41.0 18-19 38.25664852421462 40.0 38.0 41.0 33.0 41.0 20-21 38.20271196708883 40.0 37.5 41.0 33.0 41.0 22-23 38.08749036999596 40.0 37.0 41.0 33.0 41.0 24-25 37.96648401928854 40.0 37.0 41.0 33.0 41.0 26-27 37.75286556310557 40.0 37.0 41.0 32.0 41.0 28-29 37.622584601368786 40.0 37.0 41.0 32.0 41.0 30-31 37.36997927765798 39.0 36.0 41.0 31.5 41.0 32-33 37.26709491311597 39.0 36.0 41.0 31.0 41.0 34-35 37.14067474228275 39.0 36.0 41.0 31.0 41.0 36-37 37.01006628398016 39.0 36.0 41.0 31.0 41.0 38-39 36.7940263148777 39.0 35.5 41.0 30.0 41.0 40-41 36.58059077781899 39.0 35.0 40.5 30.0 41.0 42-43 36.32425115254334 39.0 35.0 40.0 30.0 41.0 44-45 36.049577961251714 38.0 35.0 40.0 29.5 41.0 46-47 35.66532398594529 38.0 35.0 40.0 28.5 41.0 48-49 35.26411305115214 38.0 34.0 40.0 27.0 41.0 50-51 34.871773821267126 38.0 34.0 40.0 26.0 41.0 52-53 34.28118108687588 38.0 33.0 40.0 24.0 41.0 54-55 33.857553770070155 37.0 33.0 40.0 23.5 41.0 56-57 33.470990886653325 37.0 32.0 39.5 23.0 41.0 58-59 32.94312501783334 36.0 31.5 39.0 21.5 40.0 60-61 32.45940125322529 36.0 31.0 39.0 19.5 40.0 62-63 31.97459729768839 35.0 30.5 38.5 18.0 40.0 64-65 31.34155305979366 35.0 29.5 38.0 13.5 40.0 66-67 30.706941398623062 34.0 29.0 38.0 8.5 40.0 68-69 30.00178779241584 34.0 28.0 37.0 7.5 39.0 70-71 29.23340263709253 34.0 27.0 36.0 4.0 39.0 72-73 28.671091288362064 33.0 26.0 36.0 2.0 39.0 74-75 27.98970519449551 32.5 26.0 35.0 2.0 38.0 76-77 26.831177749493534 31.0 25.0 34.0 2.0 36.0 78-79 27.34953373456652 32.0 26.0 35.0 2.0 37.0 80-81 26.99928157112752 32.0 25.0 35.0 2.0 36.0 82-83 26.34764862306228 32.0 24.0 35.0 2.0 36.0 84-85 25.60260170609024 31.0 21.0 34.0 2.0 35.0 86-87 24.745999472133114 31.0 19.0 34.0 2.0 35.0 88-89 23.79710651497797 30.0 11.5 34.0 2.0 35.0 90-91 22.979127603158233 30.0 4.5 34.0 2.0 35.0 92-93 21.94997875284824 29.0 2.0 34.0 2.0 35.0 94-95 20.81472445450358 28.5 2.0 33.5 2.0 35.0 96-97 19.47077892465974 26.5 2.0 33.0 2.0 34.0 98-99 17.71704793500104 22.5 2.0 32.5 2.0 34.0 100-101 13.34539168640223 2.0 2.0 28.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3532607065814446 1101 2 0.33274778368704006 1101 3 0.31055646500561096 1101 4 0.24109984803816786 1101 5 0.2095826146614712 1101 6 0.2398907329590969 1101 7 0.23717017786205474 1101 8 0.30150680268558716 1101 9 0.3835443535840568 1101 10-11 0.2916938215474829 1101 12-13 0.3550216319818631 1101 14-15 0.5287780161366911 1101 16-17 0.4083470220047687 1101 18-19 0.4078168876155175 1101 20-21 0.5347712456398526 1101 22-23 0.43703957510999203 1101 24-25 0.4768595413229235 1101 26-27 0.5319012633616751 1101 28-29 0.6343457432299218 1101 30-31 0.6036286659249015 1101 32-33 0.55763083208452 1101 34-35 0.36721462918450953 1101 36-37 0.5077139195011497 1101 38-39 0.6666548994117818 1101 40-41 0.624640571484754 1101 42-43 0.5501471491447703 1101 44-45 0.7939533723536627 1101 46-47 0.7919942457426785 1101 48-49 0.6151117800193973 1101 50-51 0.7917194636997849 1101 52-53 0.8946409340473949 1101 54-55 0.8449088020592512 1101 56-57 0.9050326596499403 1101 58-59 0.924562015327048 1101 60-61 1.073289587044485 1101 62-63 1.1384088361256808 1101 64-65 1.2294254982282062 1101 66-67 1.3688020791907718 1101 68-69 1.5737215981781603 1101 70-71 1.6505722177419493 1101 72-73 1.6672829120615056 1101 74-75 1.6661617548774252 1101 76-77 1.6924887096121672 1101 78-79 1.6489976394524 1101 80-81 1.7910831447377618 1101 82-83 2.0797925093016154 1101 84-85 2.3364156325146475 1101 86-87 2.291292451850829 1101 88-89 2.1909364269410005 1101 90-91 2.393562249098803 1101 92-93 2.5890901730758777 1101 94-95 2.4287193074206535 1101 96-97 2.548651131331887 1101 98-99 2.604137580628702 1101 100-101 2.3497669592114985 1102 1 0.2242686140587722 1102 2 0.17672100240428534 1102 3 0.23288888750312253 1102 4 0.16300785381896077 1102 5 0.20669000299731977 1102 6 0.2721216933618962 1102 7 0.25089619450454137 1102 8 0.24568028372150508 1102 9 0.264944889553945 1102 10-11 0.34131960461166244 1102 12-13 0.3475479015532059 1102 14-15 0.4008711642308569 1102 16-17 0.30916306773440994 1102 18-19 0.3734773489592911 1102 20-21 0.30557884819494063 1102 22-23 0.3065975554521998 1102 24-25 0.3480417825891209 1102 26-27 0.47157065287900224 1102 28-29 0.3904274660703777 1102 30-31 0.3275649337179587 1102 32-33 0.30126711624586733 1102 34-35 0.489887590499535 1102 36-37 0.5436111297089283 1102 38-39 0.5629075068564902 1102 40-41 0.5052458693062505 1102 42-43 0.5003513778553668 1102 44-45 0.4320156787044809 1102 46-47 0.4438731013120858 1102 48-49 0.5742898532936138 1102 50-51 0.6790395946426955 1102 52-53 0.6446258736870902 1102 54-55 0.5604081154175873 1102 56-57 0.6726920291495659 1102 58-59 0.8220322326657268 1102 60-61 0.749345326822592 1102 62-63 0.872420889696766 1102 64-65 1.0608889042792988 1102 66-67 1.2155247189894745 1102 68-69 1.232470193432107 1102 70-71 1.3584568254743097 1102 72-73 1.570090972690128 1102 74-75 1.3783939286297588 1102 76-77 1.2619976617715167 1102 78-79 1.5155910933123558 1102 80-81 1.7932157514019629 1102 82-83 1.6646528893237154 1102 84-85 1.641123488364972 1102 86-87 1.5920999513052863 1102 88-89 1.402050938763363 1102 90-91 1.7793197664765863 1102 92-93 2.0822782464293326 1102 94-95 1.8993322202034015 1102 96-97 2.0430989495053247 1102 98-99 1.9159947833621125 1102 100-101 1.952472566786481 1103 1 0.18567197324706797 1103 2 0.2376206683515818 1103 3 0.22206543512111665 1103 4 0.16950100827229164 1103 5 0.15422072370013495 1103 6 0.14334168150556081 1103 7 0.24203418569996415 1103 8 0.23317595020611748 1103 9 0.2189177679814449 1103 10-11 0.3349414743254755 1103 12-13 0.3572850994840522 1103 14-15 0.4702832218201607 1103 16-17 0.33375249982872646 1103 18-19 0.37189886346561707 1103 20-21 0.43180938558302273 1103 22-23 0.49811251221357367 1103 24-25 0.34005958800845093 1103 26-27 0.43763639200579263 1103 28-29 0.4576895369719196 1103 30-31 0.4849196030958751 1103 32-33 0.48324516191286904 1103 34-35 0.5313007146765756 1103 36-37 0.5236862895563306 1103 38-39 0.5106162737089335 1103 40-41 0.6004847535879207 1103 42-43 0.5861875995286567 1103 44-45 0.7553629302301559 1103 46-47 0.8055519956643948 1103 48-49 0.7922964040405844 1103 50-51 0.7976018408153251 1103 52-53 0.9736527048838965 1103 54-55 1.0709725623323152 1103 56-57 1.1273084396394424 1103 58-59 1.1143734731732096 1103 60-61 1.1792742531484066 1103 62-63 1.2631270174282854 1103 64-65 1.378877477472873 1103 66-67 1.3062363968381518 1103 68-69 1.288588433449739 1103 70-71 1.6157449336645904 1103 72-73 1.3031456204306266 1103 74-75 1.3208010063965538 1103 76-77 1.4128610267351576 1103 78-79 1.53267278540633 1103 80-81 1.5926563156799638 1103 82-83 1.861015390801736 1103 84-85 1.858676658889511 1103 86-87 1.6969311271981553 1103 88-89 1.7905468328832157 1103 90-91 2.0189030411583175 1103 92-93 2.278535215244915 1103 94-95 1.8918177637569364 1103 96-97 1.852954871681419 1103 98-99 1.9395315432358906 1103 100-101 1.8573690571015273 1104 1 0.22154494415615744 1104 2 0.2478158642556707 1104 3 0.21090546320858294 1104 4 0.15106834587570006 1104 5 0.14676817750532223 1104 6 0.1834371399240453 1104 7 0.24480816027885766 1104 8 0.18789682538400854 1104 9 0.19002567166571538 1104 10-11 0.31716168815892587 1104 12-13 0.26237520344814413 1104 14-15 0.39344005674934124 1104 16-17 0.302451236267423 1104 18-19 0.4374177509381383 1104 20-21 0.44233840118025824 1104 22-23 0.3693033368744878 1104 24-25 0.36517598837625087 1104 26-27 0.4512187580526401 1104 28-29 0.4046705839270004 1104 30-31 0.3521295089489058 1104 32-33 0.4671542208703201 1104 34-35 0.38815130628945127 1104 36-37 0.36589782535173043 1104 38-39 0.39087332498954197 1104 40-41 0.3554786347467598 1104 42-43 0.4008905415933839 1104 44-45 0.3855086208498548 1104 46-47 0.36366176460226285 1104 48-49 0.49728878640183183 1104 50-51 0.46792227624600713 1104 52-53 0.5180217972795234 1104 54-55 0.7112966623231074 1104 56-57 0.7282868172360182 1104 58-59 0.7140177335653135 1104 60-61 0.6937600943822773 1104 62-63 0.7647453441256964 1104 64-65 1.024477270039288 1104 66-67 0.8850996038303407 1104 68-69 1.024353153702915 1104 70-71 1.094488199379672 1104 72-73 1.285945793991388 1104 74-75 1.2236946599629803 1104 76-77 1.187932275825574 1104 78-79 1.3817860218124274 1104 80-81 1.489740704728849 1104 82-83 1.6151190611322122 1104 84-85 1.7109464004893091 1104 86-87 1.6208026262880075 1104 88-89 1.6572030829066335 1104 90-91 1.8771696217939997 1104 92-93 1.9421450842275227 1104 94-95 1.8241496005408813 1104 96-97 1.9799473544621087 1104 98-99 2.2533619977537533 1104 100-101 2.0191007078846877 1105 1 0.1635873882524379 1105 2 0.16769622317890054 1105 3 0.14770965381137557 1105 4 0.17178840791181216 1105 5 0.08781804377951374 1105 6 0.1480005924236849 1105 7 0.12563678551426705 1105 8 0.12752809031935897 1105 9 0.16559863531445274 1105 10-11 0.2655337149357422 1105 12-13 0.24591600960111037 1105 14-15 0.31624149370830423 1105 16-17 0.23686608695346223 1105 18-19 0.272900192502334 1105 20-21 0.19422622975802284 1105 22-23 0.26554255572586527 1105 24-25 0.26355513664573493 1105 26-27 0.24912345665524072 1105 28-29 0.35999350405550246 1105 30-31 0.4305292304194168 1105 32-33 0.5113726766142079 1105 34-35 0.4642433476257537 1105 36-37 0.3933915346998802 1105 38-39 0.3240939804833829 1105 40-41 0.3999338405578996 1105 42-43 0.40033601469787783 1105 44-45 0.406780994205981 1105 46-47 0.41603955187536457 1105 48-49 0.46920921559046036 1105 50-51 0.551893537066718 1105 52-53 0.5859437457199164 1105 54-55 0.5886873474106977 1105 56-57 0.7050514439906692 1105 58-59 0.820698959728098 1105 60-61 0.841118337114402 1105 62-63 0.892471055879323 1105 64-65 1.0909163654514593 1105 66-67 1.0504711083643592 1105 68-69 0.9997608987218243 1105 70-71 1.0274537471807115 1105 72-73 1.2260287198170872 1105 74-75 1.2380591854341851 1105 76-77 1.2294692521980437 1105 78-79 1.2707030801611872 1105 80-81 1.3797351657901125 1105 82-83 1.5977397704328844 1105 84-85 1.7628902514760334 1105 86-87 1.7856525210481706 1105 88-89 1.81315174485729 1105 90-91 1.9869624654851528 1105 92-93 2.0996250253565165 1105 94-95 1.8985033646453928 1105 96-97 1.988488397998168 1105 98-99 2.14944834189593 1105 100-101 2.0038736905240704 1106 1 -0.029330150807872712 1106 2 -6.190509692132196E-4 1106 3 0.002540702364861147 1106 4 0.04999726277097949 1106 5 0.04153660576913154 1106 6 -0.0614747487650078 1106 7 -0.03956976164084125 1106 8 -0.05519703818358579 1106 9 -0.16140089091848608 1106 10-11 0.036822004738546354 1106 12-13 -0.0062954637125542945 1106 14-15 -0.08126129540422511 1106 16-17 -0.08780872035580245 1106 18-19 0.030470022200908886 1106 20-21 0.061451024586311576 1106 22-23 0.054857019885133695 1106 24-25 0.04883993177968193 1106 26-27 -0.19722267857723352 1106 28-29 0.03455170353690562 1106 30-31 0.09948577120668034 1106 32-33 -0.06689527177065457 1106 34-35 -0.03888392250806305 1106 36-37 -0.010554995847904536 1106 38-39 0.03249045728662736 1106 40-41 0.1591059198858602 1106 42-43 0.2103408542538645 1106 44-45 0.14140582673187652 1106 46-47 -0.2597672530497732 1106 48-49 -0.0164650873861234 1106 50-51 0.2320627686980501 1106 52-53 0.06925634537655867 1106 54-55 0.03418610186182036 1106 56-57 0.2832180164133504 1106 58-59 0.5670564042387625 1106 60-61 0.4812834933301744 1106 62-63 0.329323714323845 1106 64-65 0.699946365604287 1106 66-67 0.4959366481786205 1106 68-69 0.3334603062582886 1106 70-71 0.25816781093039864 1106 72-73 0.5679667836890374 1106 74-75 0.5395743230558772 1106 76-77 0.5342319865981153 1106 78-79 0.752970030069239 1106 80-81 0.7904975232142455 1106 82-83 0.9004966871772346 1106 84-85 1.0314347677496762 1106 86-87 1.0790763286070515 1106 88-89 1.1920184736740431 1106 90-91 1.2168403558462835 1106 92-93 1.2414138271333996 1106 94-95 1.2331717022324398 1106 96-97 1.381894885040893 1106 98-99 1.5883359572174562 1106 100-101 1.6433847425416541 1107 1 0.05801767470925867 1107 2 0.01995510101291842 1107 3 0.009785059641366445 1107 4 0.07956812513995004 1107 5 0.028068426820965442 1107 6 -0.011518229942055314 1107 7 -0.01637112744320035 1107 8 0.04096285690651058 1107 9 0.1419418016611118 1107 10-11 0.009854616392267701 1107 12-13 0.08898218364738852 1107 14-15 0.11383851471255468 1107 16-17 0.11045494287169078 1107 18-19 0.06094105672141126 1107 20-21 0.08079340262123225 1107 22-23 0.07922363250870745 1107 24-25 0.16768674774714043 1107 26-27 0.14849604446386167 1107 28-29 0.12145614054431775 1107 30-31 0.08343792109648263 1107 32-33 0.14930871354133757 1107 34-35 0.007712345511762919 1107 36-37 0.08654236575436158 1107 38-39 0.09848557490551713 1107 40-41 0.09045972252295087 1107 42-43 0.061971456213747445 1107 44-45 0.13025037998205136 1107 46-47 0.17904744543833573 1107 48-49 0.17544658491998888 1107 50-51 0.05484266528377901 1107 52-53 0.009479363725773737 1107 54-55 0.12926918251862674 1107 56-57 0.17567177718824212 1107 58-59 0.26598134370048143 1107 60-61 0.2686710416779263 1107 62-63 0.44276313710350834 1107 64-65 0.569184242335151 1107 66-67 0.5701475110718306 1107 68-69 0.686004668237544 1107 70-71 0.6012311176739153 1107 72-73 0.8416083245571002 1107 74-75 0.7807402816115285 1107 76-77 0.780434225724548 1107 78-79 0.8980188808632725 1107 80-81 0.9789183662710919 1107 82-83 1.0401152092335266 1107 84-85 1.21455730603277 1107 86-87 1.5125114842180096 1107 88-89 1.7387154461043544 1107 90-91 1.678554948175556 1107 92-93 1.7326737443495546 1107 94-95 1.4623202999259313 1107 96-97 1.4012194350222735 1107 98-99 1.595767749975682 1107 100-101 1.4385422384575843 1108 1 -0.02728445347808872 1108 2 0.07559741936880471 1108 3 0.04700814236365858 1108 4 0.0582119429756105 1108 5 0.049740614072746325 1108 6 0.09501938072209981 1108 7 0.050476244237941614 1108 8 0.041567959129402254 1108 9 0.008640936068530891 1108 10-11 0.06516495120797572 1108 12-13 0.02067236774529846 1108 14-15 0.187467937684886 1108 16-17 0.1410959425597227 1108 18-19 0.029761838594033918 1108 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86-87 1.4758721496154301 1108 88-89 1.6473780365429924 1108 90-91 1.8023039660056064 1108 92-93 1.8634556276590928 1108 94-95 1.6666972733242424 1108 96-97 1.7481958425101247 1108 98-99 1.9622447007309205 1108 100-101 1.7837257328703533 1109 1 0.18879683335759267 1109 2 0.17795735614426889 1109 3 0.26651122731792043 1109 4 0.17135050957985243 1109 5 0.21759675671621892 1109 6 0.20340570936937752 1109 7 0.2342526486557901 1109 8 0.2902613972242847 1109 9 0.27942597651532 1109 10-11 0.3338843792035249 1109 12-13 0.36421300387835487 1109 14-15 0.5238986156550567 1109 16-17 0.3450534033522885 1109 18-19 0.4564068351801396 1109 20-21 0.4278132757739357 1109 22-23 0.5403541516965547 1109 24-25 0.48577756216337775 1109 26-27 0.5443104376166232 1109 28-29 0.6105967832106032 1109 30-31 0.4718541066633293 1109 32-33 0.5353557421058284 1109 34-35 0.6258100437702581 1109 36-37 0.609267890426203 1109 38-39 0.6792909229202806 1109 40-41 0.7186412882855677 1109 42-43 0.7186452067002378 1109 44-45 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69.0 5 139.0 6 275.0 7 726.0 8 1826.0 9 3324.0 10 5350.0 11 8305.0 12 12031.0 13 16598.0 14 22257.0 15 28949.0 16 35989.0 17 44516.0 18 45345.0 19 43661.0 20 45155.0 21 48764.0 22 53564.0 23 60032.0 24 67937.0 25 78236.0 26 90131.0 27 105815.0 28 124953.0 29 146477.0 30 172166.0 31 202126.0 32 238891.0 33 288112.0 34 352989.0 35 436209.0 36 514289.0 37 466339.0 38 159855.0 39 3796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22964269360053 18.919042198579664 15.078769883588809 23.772545224231 2 15.886725802613267 20.03693491189994 39.155083315030645 24.921255970456148 3 19.405675334081838 23.19048823729778 30.05545505012406 27.348381378496324 4 13.567478304467098 16.72250200752465 36.08721726511961 33.62280242288864 5 13.939950402805243 37.09723934085766 34.34019782830118 14.622612428035916 6 33.3072032430185 36.00408842076086 16.56019312998569 14.128515206234942 7 29.273377981225057 32.26232232897316 20.036216967557586 18.428082722244188 8 26.923135714340308 34.87780333891262 19.22187999174571 18.977180955001362 9 26.197182524477636 16.54996820557136 19.73009892958757 37.52275034036344 10-11 25.179352456109598 26.231736111394184 27.217091881448024 21.371819551048194 12-13 26.15914167621175 23.300915716574206 27.449969836177836 23.08997277103621 14-15 22.488135733123546 25.088109441683958 25.540923440958395 26.882831384234102 16-17 22.45282571832697 28.135763694986693 26.6737991537825 22.73761143290384 18-19 22.61215388033115 26.61469436711002 28.68016463740232 22.092987115156507 20-21 22.734274050552937 25.91142383431094 28.99483393593041 22.359468179205713 22-23 22.570640410554077 25.825912965144482 28.98088571579973 22.622560908501715 24-25 22.62085463814306 26.10316241080004 28.88304200837161 22.392940942685286 26-27 22.051403694317273 26.479129494740366 28.624703909125675 22.844762901816686 28-29 22.325953111939427 26.957042673520803 28.35011073261862 22.366893481921146 30-31 22.428470470025722 26.383141174840112 28.267794107456577 22.920594247677588 32-33 21.99542929553804 26.363367820187193 28.36060848124135 23.280594403033415 34-35 22.862044971549135 26.573269157419787 28.108492148845926 22.45619372218515 36-37 23.08791031855338 25.955955672883448 28.208246139924213 22.74788786863896 38-39 22.41987918146705 26.402370711455493 28.12623495787862 23.05151514919884 40-41 23.0223886301328 26.19450616088627 28.23298749477675 22.55011771420418 42-43 22.679227794820868 26.328827962361455 27.972234019758886 23.01971022305879 44-45 22.62651463696863 26.46243578030968 28.302750587358904 22.608298995362784 46-47 23.09734228075756 26.779346363834033 27.432085766260876 22.691225589147532 48-49 22.697550106592427 26.910001752762643 27.32511097433222 23.06733716631272 50-51 22.883055065280217 27.025485372584345 27.484439136336402 22.607020425799035 52-53 23.38748384808847 26.54808683919829 27.478350324261086 22.586078988452147 54-55 22.62289209911669 26.838439103726863 27.709636016418905 22.829032780737545 56-57 22.761049537963615 26.9384841456505 27.414799940487594 22.885666375898293 58-59 22.702581656319932 26.584186616230582 27.369605159644067 23.34362656780542 60-61 22.23367943601805 26.86343125705691 27.858952031370375 23.04393727555467 62-63 22.487498828094747 26.87653876254958 27.877778944021653 22.75818346533402 64-65 22.110258507144803 26.91767106640885 27.667104857549752 23.3049655688966 66-67 22.047037174656314 26.955303971286153 27.5819321466381 23.41572670741943 68-69 22.850131738711514 26.45308741427812 27.67928158455353 23.017499262456838 70-71 22.456767512536207 26.643056166630704 27.25044717113464 23.64972914969845 72-73 22.41939985358803 26.748539669886483 27.337397558433267 23.49466291809222 74-75 22.409070342848526 27.13766982435688 27.144306374756955 23.30895345803764 76-77 22.428546898629175 26.583078401480474 27.53311141863717 23.455263281253185 78-79 22.535750753076506 26.874870071374126 27.39190957375258 23.197469601796787 80-81 22.778542489389785 26.863414523542207 27.075032857972293 23.283010129095718 82-83 22.6110484602081 26.936903301101765 27.253881627880645 23.19816661080949 84-85 22.459990053564002 27.03137611387854 27.20057253153102 23.308061301026438 86-87 22.631491587221173 27.02162929302799 26.82211695305855 23.52476216669229 88-89 22.325266379159245 27.214977356752417 26.55907981999535 23.900676444092987 90-91 22.23564834612191 27.074734213062907 26.91406656529561 23.775550875519578 92-93 22.397388981792577 26.69350088606679 27.099695506892367 23.80941462524827 94-95 22.044494468412612 26.42013670793007 27.269717605047045 24.265651218610273 96-97 22.2475384894447 26.027136739943014 27.356370273145636 24.368954497466646 98-99 21.8868719097368 26.478294786142577 26.880895192946557 24.753938111174065 100-101 22.10639604476731 25.97579938299165 27.28159531013529 24.636209262105755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 203.0 2 184.5 3 241.5 4 239.0 5 245.5 6 447.5 7 1020.5 8 1677.0 9 1930.5 10 1763.5 11 1591.5 12 1660.0 13 1855.5 14 2094.0 15 2390.0 16 2769.5 17 3125.5 18 3527.0 19 4093.0 20 4878.0 21 5952.0 22 7262.0 23 9130.0 24 11532.0 25 14639.5 26 19372.0 27 25183.0 28 30241.0 29 35415.5 30 41502.0 31 51505.5 32 66746.0 33 72182.0 34 70988.5 35 75753.5 36 84171.5 37 92681.0 38 98148.0 39 102387.0 40 109487.5 41 118679.5 42 123822.0 43 127699.5 44 133353.0 45 141674.5 46 146238.0 47 149257.5 48 151590.5 49 148690.0 50 145546.0 51 143173.0 52 141356.0 53 142198.0 54 143871.5 55 136242.0 56 124241.5 57 113221.5 58 104641.0 59 92788.5 60 74258.0 61 61381.0 62 52616.5 63 39324.0 64 27248.0 65 20508.5 66 15325.0 67 11874.5 68 9111.0 69 6798.5 70 5168.5 71 3557.5 72 2474.0 73 1980.0 74 1543.5 75 1136.5 76 834.5 77 573.0 78 368.5 79 220.0 80 118.5 81 72.0 82 45.5 83 24.5 84 14.0 85 9.5 86 7.5 87 7.0 88 3.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02427881969779111 2 0.012483338564446637 3 0.0018088102817871655 4 0.0 5 2.547620115193191E-4 6 0.0 7 0.0 8 5.095240230386382E-5 9 4.076192184309106E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015795244714197785 26-27 0.008636432190504919 28-29 2.8023821267125103E-4 30-31 0.0 32-33 4.203573190068766E-4 34-35 0.010063099455013104 36-37 0.0012738100575965955 38-39 0.01281452917942175 40-41 0.012024766943711861 42-43 0.005426430845361497 44-45 8.66190839165685E-4 46-47 1.4011910633562552E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.095240230386382E-5 66-67 4.458335201588084E-4 68-69 9.680956437734126E-4 70-71 2.547620115193191E-5 72-73 1.6559530748755743E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2738100575965955E-4 82-83 0.0010827385489571063 84-85 0.005299049839601838 86-87 0.004114406486037003 88-89 0.0 90-91 0.0012355957558686976 92-93 5.095240230386382E-4 94-95 6.369050287982977E-5 96-97 0.0 98-99 0.0 100-101 3.8214301727897865E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3925232.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.31442697011459 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52834220412373 31.50636985161901 2 15.438882965057408 13.992082692404804 3 5.975241949555925 8.122939948557512 4 2.9238085381979815 5.2996283391507975 5 1.630832035581336 3.6950109588436884 6 1.0403929504389202 2.82868862237319 7 0.6850562599952251 2.1730052304781426 8 0.48711825120318847 1.7658787535965454 9 0.34883882711089886 1.4226688399911531 >10 1.7583775330274234 14.034311232921961 >50 0.1153261892226887 3.591561849216776 >100 0.05861400323071234 4.945154453665129 >500 0.005230989440926471 1.6347044924208063 >1k 0.0034873262349230795 3.647295441237683 >5k 4.4997757869975224E-4 1.3406992935229238 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8720 0.22215247404484628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7221 0.18396364851810035 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6562 0.1671748319589772 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 6524 0.1662067363152038 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 6328 0.16121340088942512 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6068 0.15458958858992283 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5828 0.14847530031345918 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5350 0.13629767616283572 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4869 0.12404362340875648 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4818 0.12274433715000796 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4653 0.11854076395993918 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 4337 0.1104902843959287 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 4193 0.10682171143005051 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4135 0.10534409176323845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.547620115193191E-5 0.0 0.0 0.0 0.0 3 2.547620115193191E-5 0.0 0.0 0.0 0.0 4 2.547620115193191E-5 0.0 0.0 0.0 0.0 5 2.547620115193191E-5 0.0 0.0 0.0 5.095240230386382E-5 6 2.547620115193191E-5 0.0 0.0 0.0 1.2738100575965955E-4 7 5.095240230386382E-5 0.0 0.0 0.0 1.2738100575965955E-4 8 7.642860345579573E-5 0.0 0.0 0.0 1.2738100575965955E-4 9 7.642860345579573E-5 0.0 0.0 0.0 1.2738100575965955E-4 10-11 1.2738100575965955E-4 0.0 0.0 3.8214301727897865E-5 1.401191063356255E-4 12-13 1.2738100575965955E-4 0.0 0.0 6.369050287982977E-5 2.547620115193191E-4 14-15 1.2738100575965955E-4 0.0 0.0 8.916670403176169E-5 2.92976313247217E-4 16-17 1.2738100575965955E-4 0.0 0.0 1.0190480460772765E-4 3.3119061497511486E-4 18-19 1.401191063356255E-4 0.0 0.0 1.146429051836936E-4 3.3119061497511486E-4 20-21 1.5285720691159146E-4 0.0 0.0 1.7833340806352338E-4 3.694049167030127E-4 22-23 1.7833340806352338E-4 0.0 0.0 2.92976313247217E-4 4.076192184309106E-4 24-25 2.547620115193191E-4 0.0 0.0 5.47738324766536E-4 4.076192184309106E-4 26-27 2.547620115193191E-4 0.0 0.0 0.0010317861466532423 4.4583352015880847E-4 28-29 3.05714413823183E-4 0.0 0.0 0.0029552393336241016 4.585716207347744E-4 30-31 3.3119061497511486E-4 0.0 0.0 0.011298695210881803 4.585716207347744E-4 32-33 3.3119061497511486E-4 0.0 0.0 0.029819893448336302 5.350002241905701E-4 34-35 3.3119061497511486E-4 0.0 0.0 0.057410619295878554 5.604764253425021E-4 36-37 3.5666681612704675E-4 0.0 0.0 0.10543325846727022 5.604764253425021E-4 38-39 4.585716207347744E-4 0.0 0.0 0.18121221879369168 5.73214525918468E-4 40-41 4.585716207347744E-4 0.0 0.0 0.2637041581236472 5.85952626494434E-4 42-43 4.585716207347744E-4 0.0 0.0 0.3467310976777933 6.496431293742638E-4 44-45 4.840478218867063E-4 0.0 0.0 0.4538203092199391 6.623812299502297E-4 46-47 5.73214525918468E-4 2.547620115193191E-5 0.0 0.5821439344222201 6.623812299502297E-4 48-49 5.85952626494434E-4 2.547620115193191E-5 0.0 0.7161742286825339 7.515479339819913E-4 50-51 5.85952626494434E-4 2.547620115193191E-5 0.0 0.8607644083203234 8.152384368618212E-4 52-53 5.85952626494434E-4 2.547620115193191E-5 0.0 0.999380418787985 8.152384368618212E-4 54-55 5.85952626494434E-4 2.547620115193191E-5 0.0 1.154237507490003 8.40714638013753E-4 56-57 6.114288276463658E-4 2.547620115193191E-5 0.0 1.3360229408096134 8.40714638013753E-4 58-59 6.114288276463658E-4 2.547620115193191E-5 0.0 1.5215788518997093 8.40714638013753E-4 60-61 6.241669282223318E-4 2.547620115193191E-5 0.0 1.730063344026544 8.40714638013753E-4 62-63 6.369050287982977E-4 2.547620115193191E-5 0.0 1.938586050455107 8.916670403176169E-4 64-65 6.369050287982977E-4 2.547620115193191E-5 0.0 2.1547643553298252 9.171432414695488E-4 66-67 6.623812299502297E-4 2.547620115193191E-5 0.0 2.4104562481911893 9.171432414695488E-4 68-69 6.623812299502297E-4 2.547620115193191E-5 0.0 2.6925924378482597 9.171432414695488E-4 70-71 7.005955316781276E-4 2.547620115193191E-5 0.0 2.988447561825645 9.426194426214807E-4 72-73 7.260717328300594E-4 2.547620115193191E-5 0.0 3.300492811635083 9.935718449253445E-4 74-75 7.388098334060254E-4 2.547620115193191E-5 0.0 3.612143180326666 0.0010700004483811401 76-77 7.388098334060254E-4 2.547620115193191E-5 0.0 3.948212487822376 0.0011209528506850041 78-79 7.388098334060254E-4 3.8214301727897865E-5 0.0 4.3320624105785335 0.0011209528506850041 80-81 8.152384368618212E-4 5.095240230386382E-5 1.2738100575965956E-5 4.733809364643925 0.0011209528506850041 82-83 8.152384368618212E-4 5.095240230386382E-5 2.547620115193191E-5 5.151402515825816 0.001146429051836936 84-85 8.152384368618212E-4 5.095240230386382E-5 2.547620115193191E-5 5.576931503666534 0.001146429051836936 86-87 8.152384368618212E-4 5.095240230386382E-5 2.547620115193191E-5 6.010702042579903 0.001171905252988868 88-89 8.152384368618212E-4 5.095240230386382E-5 2.547620115193191E-5 6.4684584248778165 0.0011973814541407998 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6970 0.0 45.33127 1 GTATCAA 11340 0.0 37.289448 1 ATCAACG 14385 0.0 28.990843 3 TATCAAC 14660 0.0 28.677103 2 TCAACGC 14740 0.0 28.35671 4 CAACGCA 14840 0.0 28.197636 5 AACGCAG 15580 0.0 26.857994 6 ACGCAGA 17725 0.0 23.554167 7 TAAGGTG 2835 0.0 23.455551 5 CGCAGAG 17995 0.0 23.068787 8 GAGTACT 11765 0.0 21.215141 12-13 GTACATG 11960 0.0 21.015198 1 AGGTAAG 2765 0.0 20.78817 2 GTAAGGT 2875 0.0 20.485874 4 TACATGG 12020 0.0 20.471575 2 AAGGTAA 2750 0.0 20.387234 1 GTGTAGC 2290 0.0 20.332912 1 GCAGAGT 20325 0.0 20.260662 9 GTACTTT 12245 0.0 20.170176 14-15 AGAGTAC 16955 0.0 20.127697 10-11 >>END_MODULE