FastQCFastQC Report
Thu 26 May 2016
SRR938563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737447
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.4442353145378583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30950.4196911778066763No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19880.26957869514690547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.2638833706015483No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13510.18319960620898856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11270.15282454196708373No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8570.11621174131835915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.10888918118861424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG600.00392975231.6999915
CGCCGGT300.003948694631.67095294-95
TGTAGCG700.00834670927.1714235
GTATAGA2900.024.5948241
GTATAGC4550.024.0362591
TAGGACG1452.3139775E-522.9551684
GTATAGT2205.5701094E-821.6136321
GAGTACT29350.021.04988712-13
TAGCCCT8750.020.6502824
TCGCCGG704.8482034E-420.359994-95
AGTACTT28400.020.2479712-13
ATAGCCC4500.020.076663
GTACTTT30450.019.96918514-15
TATAGCC5750.019.0199972
GTACATG23150.018.6913571
GTGTAGC5750.018.1930391
CGGGTCC800.001189879517.81551634-35
GTAAGCG951.8641973E-417.50236994-95
GTCCTAC2453.7204845E-617.4673421
AGAGTAC44300.017.43327110-11
Produced by FastQC (version 0.11.5)