##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 737447 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.217429862756237 31.0 31.0 34.0 28.0 34.0 2 31.26827148256078 31.0 31.0 34.0 28.0 34.0 3 31.304549343885054 31.0 31.0 34.0 28.0 34.0 4 34.93392203100697 37.0 35.0 37.0 32.0 37.0 5 34.93396135586693 37.0 35.0 37.0 32.0 37.0 6 34.964721532530476 37.0 35.0 37.0 32.0 37.0 7 34.897237360786605 37.0 35.0 37.0 32.0 37.0 8 34.88718918105301 37.0 35.0 37.0 32.0 37.0 9 36.40659328738201 39.0 37.0 39.0 32.0 39.0 10-11 36.275223168580254 39.0 35.0 39.0 32.0 39.0 12-13 36.182416499084 39.0 35.0 39.0 32.0 39.0 14-15 37.19409937256508 39.5 36.5 41.0 31.5 41.0 16-17 37.047503074797234 39.0 36.0 41.0 31.0 41.0 18-19 37.210613779702136 39.5 37.0 41.0 32.0 41.0 20-21 37.22635118184765 40.0 37.0 41.0 31.0 41.0 22-23 37.149418873491925 40.0 36.5 41.0 31.0 41.0 24-25 37.0475905387099 39.0 36.0 41.0 31.0 41.0 26-27 36.869574355852016 39.0 36.0 41.0 30.5 41.0 28-29 36.70110936785966 39.0 36.0 41.0 30.0 41.0 30-31 36.48390799610006 39.0 36.0 40.5 30.0 41.0 32-33 36.23556269128493 39.0 35.0 40.0 30.0 41.0 34-35 36.01281990434567 38.5 35.0 40.0 29.5 41.0 36-37 35.77618323757504 38.0 35.0 40.0 28.5 41.0 38-39 35.657731335268835 38.0 35.0 40.0 27.0 41.0 40-41 35.59257817849961 38.0 35.0 40.0 27.0 41.0 42-43 35.34304160163374 38.0 35.0 40.0 26.5 41.0 44-45 35.00639774790595 38.0 34.0 40.0 25.0 41.0 46-47 34.633505865506265 38.0 34.0 40.0 24.0 41.0 48-49 34.314137151551236 38.0 33.0 40.0 23.0 41.0 50-51 33.64767230729801 37.5 33.0 39.5 22.5 40.5 52-53 33.696614807572615 38.0 33.0 39.5 22.0 40.5 54-55 33.86682907381819 38.0 33.0 40.0 20.5 41.0 56-57 33.611627683074175 38.0 33.0 40.0 18.5 41.0 58-59 33.33217234594486 38.0 33.0 40.0 15.5 41.0 60-61 32.93412814751433 37.5 32.0 40.0 11.0 41.0 62-63 32.474238148639834 37.0 31.0 40.0 8.0 41.0 64-65 32.0628146836315 36.5 31.0 39.0 7.5 41.0 66-67 31.604453608191506 36.0 31.0 39.0 2.0 40.0 68-69 31.111292743749722 35.5 29.5 39.0 2.0 40.0 70-71 30.54972425136993 35.0 29.0 38.5 2.0 40.0 72-73 29.963228543881797 35.0 28.5 38.0 2.0 39.5 74-75 29.349637329869132 34.0 28.0 37.0 2.0 39.0 76-77 28.642732291269745 34.0 26.5 36.5 2.0 39.0 78-79 27.892270902180087 33.5 26.0 36.0 2.0 38.5 80-81 27.21334685746908 33.0 25.5 35.5 2.0 37.0 82-83 26.51215070371159 32.0 23.5 35.0 2.0 37.0 84-85 25.819568728328953 32.0 21.0 35.0 2.0 36.0 86-87 25.09418100555023 31.0 19.0 34.5 2.0 36.0 88-89 24.30771092702255 31.0 12.5 34.0 2.0 35.0 90-91 23.437756882867514 30.5 4.5 34.0 2.0 35.0 92-93 22.540164920326475 30.0 2.0 34.0 2.0 35.0 94-95 21.572922528669856 29.0 2.0 34.0 2.0 35.0 96-97 20.360636764404767 28.5 2.0 34.0 2.0 35.0 98-99 18.85972958056647 26.0 2.0 33.5 2.0 35.0 100-101 15.02480517243951 10.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3640595198800298 1101 2 0.36331583878805773 1101 3 0.29904119847792643 1101 4 0.0549333498859923 1101 5 0.16421276028207643 1101 6 0.250354954023571 1101 7 0.1718013034272161 1101 8 0.3743555244677239 1101 9 0.3561942885860603 1101 10-11 0.44275866402146846 1101 12-13 0.5174869342188799 1101 14-15 0.6071957265830292 1101 16-17 0.3095933015154273 1101 18-19 0.4173065993372873 1101 20-21 0.3145107107069265 1101 22-23 0.4891618556915276 1101 24-25 0.49226127053866975 1101 26-27 0.4741850589576231 1101 28-29 0.5043362724083948 1101 30-31 0.49061293861138466 1101 32-33 0.5380746649868229 1101 34-35 0.6346491551811795 1101 36-37 0.7263496355590959 1101 38-39 0.7053604520019903 1101 40-41 0.8902356828350335 1101 42-43 0.8914762075712162 1101 44-45 0.9109146426872101 1101 46-47 0.8230427783807599 1101 48-49 0.6640488520621233 1101 50-51 0.6763476494391227 1101 52-53 0.5911445597849863 1101 54-55 0.6077638761673043 1101 56-57 0.7720472714552216 1101 58-59 0.6963595210831173 1101 60-61 0.8196095436341864 1101 62-63 0.8427905043608703 1101 64-65 0.673618585416726 1101 66-67 1.0042931724852089 1101 68-69 0.9160656918311538 1101 70-71 0.9151494063461776 1101 72-73 1.152729983218066 1101 74-75 0.6236968950498039 1101 76-77 0.6680308335490572 1101 78-79 0.8656650985874705 1101 80-81 0.8735223803883123 1101 82-83 1.064646552066698 1101 84-85 1.1182680819023538 1101 86-87 0.8645529153088667 1101 88-89 1.0575083172981188 1101 90-91 0.8248639975389196 1101 92-93 0.9836799105033869 1101 94-95 0.9702907363139204 1101 96-97 1.0117961210219022 1101 98-99 1.0878994529411763 1101 100-101 0.9160922743990412 1102 1 0.4525688366502152 1102 2 0.4343562114588657 1102 3 0.3360494800514253 1102 4 0.24172165216342023 1102 5 0.3460838782945004 1102 6 0.40304646541073197 1102 7 0.2865787361394325 1102 8 0.41233430293563345 1102 9 0.43829832585314676 1102 10-11 0.5421698959055306 1102 12-13 0.6014996153782945 1102 14-15 0.8939452089846895 1102 16-17 0.7052001855734034 1102 18-19 0.52901079188387 1102 20-21 0.7706442510795952 1102 22-23 0.7152552821925582 1102 24-25 0.6675977094620649 1102 26-27 0.565023568274384 1102 28-29 0.7142547506692694 1102 30-31 0.7341753092117997 1102 32-33 0.7484783916948956 1102 34-35 0.33301872039857017 1102 36-37 0.5350970475673762 1102 38-39 0.7541117459978537 1102 40-41 0.713055299812261 1102 42-43 0.5436824705111789 1102 44-45 0.7291405743642301 1102 46-47 0.2904664326250668 1102 48-49 0.4204864814617153 1102 50-51 0.41984532024657284 1102 52-53 0.2443536695158386 1102 54-55 0.3113417747180236 1102 56-57 0.6317781203165111 1102 58-59 0.7495104010003004 1102 60-61 0.6620331978784932 1102 62-63 0.7078914360378903 1102 64-65 0.5064019705099057 1102 66-67 0.32494534639824835 1102 68-69 0.8894093771313543 1102 70-71 0.6633054622468002 1102 72-73 0.5580418362201378 1102 74-75 0.38576340643697193 1102 76-77 0.3714793325138608 1102 78-79 0.2503700675315699 1102 80-81 0.4284848545083939 1102 82-83 0.765215910244752 1102 84-85 0.8219106802460381 1102 86-87 1.1450588676898228 1102 88-89 1.2232691868633374 1102 90-91 1.425116326731466 1102 92-93 1.8473897966317487 1102 94-95 1.7272459640571896 1102 96-97 1.8626630982475767 1102 98-99 2.279184390829375 1102 100-101 2.0194501936537 1103 1 0.16598596315747116 1103 2 0.1946898513525923 1103 3 0.235165564253343 1103 4 0.19516917924292443 1103 5 0.30660784109850425 1103 6 0.3082171173625028 1103 7 0.2995947794666307 1103 8 0.2889629050202416 1103 9 0.1732577066011487 1103 10-11 0.44325626929608575 1103 12-13 0.404678263446975 1103 14-15 0.4680306371473648 1103 16-17 0.6237221812815505 1103 18-19 0.634432440156921 1103 20-21 0.42409050082412847 1103 22-23 0.5492943430958874 1103 24-25 0.6113055574081088 1103 26-27 0.6100368525580606 1103 28-29 0.7516583118791544 1103 30-31 0.6427724122160967 1103 32-33 0.37940522594791304 1103 34-35 0.7223120986940899 1103 36-37 0.7169895470564427 1103 38-39 0.7316913239663734 1103 40-41 0.800664511952057 1103 42-43 1.065758012101206 1103 44-45 0.7358497762853489 1103 46-47 0.21459592884415457 1103 48-49 0.6656805086433977 1103 50-51 0.7838059527828563 1103 52-53 0.8603867780382188 1103 54-55 0.7631000263019203 1103 56-57 0.9885888703676073 1103 58-59 0.9760170175954457 1103 60-61 0.7773797388553945 1103 62-63 0.861446259254727 1103 64-65 0.9369117548957533 1103 66-67 1.0616610627276977 1103 68-69 1.1209902068881554 1103 70-71 1.2827841818462282 1103 72-73 1.23337202730945 1103 74-75 1.247035248864929 1103 76-77 1.110420805025612 1103 78-79 1.2516346291502067 1103 80-81 1.1080877310667425 1103 82-83 1.07272153051861 1103 84-85 1.2307568635691517 1103 86-87 1.5340041722064761 1103 88-89 1.0097881936076654 1103 90-91 1.1060220849575053 1103 92-93 1.1000817800774918 1103 94-95 1.0939400934256795 1103 96-97 1.8973746760117258 1103 98-99 1.7962016859448475 1103 100-101 1.5898153837211417 1104 1 0.1954066221709283 1104 2 0.15694138373915933 1104 3 0.16555973943973257 1104 4 0.07763193090907095 1104 5 0.22201545011022006 1104 6 0.2050209137266421 1104 7 0.23240239080455183 1104 8 0.11404984687678876 1104 9 0.3842792588767594 1104 10-11 0.44549814793804643 1104 12-13 0.41387258285796946 1104 14-15 0.5872282132433071 1104 16-17 0.7252289796113374 1104 18-19 0.432605207292319 1104 20-21 0.35046906718490334 1104 22-23 0.4501021172680524 1104 24-25 0.5774651117075109 1104 26-27 0.7111714583247206 1104 28-29 0.6814622129224546 1104 30-31 0.7066092523012344 1104 32-33 0.5253366959922303 1104 34-35 0.38090393139353296 1104 36-37 0.6869553518647038 1104 38-39 0.7635544966753542 1104 40-41 0.7853610005478444 1104 42-43 0.49346832997691337 1104 44-45 0.5060412228350017 1104 46-47 0.32987675976405484 1104 48-49 -0.026202204175142185 1104 50-51 0.5772507112647745 1104 52-53 0.6832812730775899 1104 54-55 0.6561382807187996 1104 56-57 0.6926085500493784 1104 58-59 0.6505520676669718 1104 60-61 0.9587509195199928 1104 62-63 1.089533422100608 1104 64-65 1.327522034389112 1104 66-67 1.144534970865923 1104 68-69 1.0882576156137453 1104 70-71 1.4351127115692925 1104 72-73 1.3543724111330313 1104 74-75 0.9371994788334064 1104 76-77 1.0043372012711203 1104 78-79 1.2953032847208839 1104 80-81 0.9885470399516798 1104 82-83 0.9691938428812215 1104 84-85 1.213394662050142 1104 86-87 0.8944116067529251 1104 88-89 0.9796813045559425 1104 90-91 0.9504804866230643 1104 92-93 0.782258408710728 1104 94-95 0.811335443344845 1104 96-97 1.0806388047903575 1104 98-99 1.3493728828928688 1104 100-101 0.9312351820392983 1105 1 0.38979392748976593 1105 2 0.27205223364284237 1105 3 0.2508276403115133 1105 4 0.29712780056655674 1105 5 0.1464938515207379 1105 6 0.10643143385594556 1105 7 0.23756123757374326 1105 8 0.24248227577938763 1105 9 0.45487056719947816 1105 10-11 0.42785848358959555 1105 12-13 0.460498109354198 1105 14-15 0.5305870143011902 1105 16-17 0.5295601253324378 1105 18-19 0.513726440191391 1105 20-21 0.6987973396242495 1105 22-23 0.6728385916207458 1105 24-25 0.7269900406917387 1105 26-27 0.5766414684465104 1105 28-29 0.434710980349891 1105 30-31 0.7011891981006926 1105 32-33 0.6797511015820632 1105 34-35 0.5553007054817556 1105 36-37 0.5836723009619078 1105 38-39 0.522623292182594 1105 40-41 0.9530480087432309 1105 42-43 0.9242075470076401 1105 44-45 0.9039499308377472 1105 46-47 0.8789184550002815 1105 48-49 0.6596305577669312 1105 50-51 0.7770090943907704 1105 52-53 0.7115080011280028 1105 54-55 0.641845336584538 1105 56-57 0.45265687342417493 1105 58-59 0.5662147182693857 1105 60-61 0.4284590202154632 1105 62-63 0.5824073568640458 1105 64-65 0.8109027115693763 1105 66-67 0.6304052813762588 1105 68-69 1.0358283865021782 1105 70-71 1.089363862514535 1105 72-73 0.6596177828638545 1105 74-75 0.6336029790129913 1105 76-77 0.937149752288196 1105 78-79 0.8734696085527958 1105 80-81 1.0643706596336564 1105 82-83 0.769925223689004 1105 84-85 0.874355578888224 1105 86-87 1.3531979717250096 1105 88-89 1.2594018126043949 1105 90-91 1.1061111393151357 1105 92-93 1.1764530735489451 1105 94-95 1.1837356131296737 1105 96-97 1.7142300804621478 1105 98-99 1.7255442349678347 1105 100-101 1.6162271825617118 1106 1 0.1548856987921141 1106 2 0.040886205690746635 1106 3 -0.032813879558908354 1106 4 0.10048479066242777 1106 5 0.18865622015183448 1106 6 -0.06135140679332807 1106 7 0.17770944818198586 1106 8 0.22705943592287525 1106 9 0.08543950062722416 1106 10-11 0.1699808537341454 1106 12-13 0.23735790384846922 1106 14-15 0.31460149303262597 1106 16-17 0.2084317355958305 1106 18-19 0.21015684552740055 1106 20-21 0.4023617092607168 1106 22-23 0.23796225360830903 1106 24-25 0.13638175740155845 1106 26-27 0.0953542739079225 1106 28-29 0.03661162915417293 1106 30-31 0.01126845636299123 1106 32-33 0.15928881276776963 1106 34-35 -0.05795176604634378 1106 36-37 -0.09273093423099965 1106 38-39 0.055332865333873826 1106 40-41 0.33640197192659826 1106 42-43 -0.3575394499528386 1106 44-45 -0.06212252407837582 1106 46-47 0.0258047890308859 1106 48-49 0.1756948147950439 1106 50-51 0.014307322746098805 1106 52-53 -0.2869704348230684 1106 54-55 -0.026362713200960286 1106 56-57 -0.6984067800231628 1106 58-59 -0.3258947694797314 1106 60-61 0.08658997381901656 1106 62-63 0.23898521608018797 1106 64-65 0.3508399391697665 1106 66-67 0.06658157020136102 1106 68-69 -0.007573574865052279 1106 70-71 -0.3695638176993654 1106 72-73 0.13814562235101135 1106 74-75 0.5091401208508657 1106 76-77 0.6568801769027601 1106 78-79 0.5504926401137631 1106 80-81 0.7852722111064843 1106 82-83 0.5352521212298207 1106 84-85 0.6687840779900611 1106 86-87 0.7741135767201364 1106 88-89 0.8573179274901399 1106 90-91 0.8864428745107382 1106 92-93 0.8493014882936087 1106 94-95 1.054780132165888 1106 96-97 0.25410424738555903 1106 98-99 0.7983784160168383 1106 100-101 0.8482301157840588 1107 1 0.19033982053014853 1107 2 0.13396942250203736 1107 3 0.1048793855544794 1107 4 -0.0250063911939975 1107 5 0.1789713620817892 1107 6 0.1453106862741791 1107 7 0.006457835639153586 1107 8 0.10208671168123118 1107 9 0.17720998974048996 1107 10-11 0.2490238193815344 1107 12-13 0.48547740396455197 1107 14-15 0.47334116970360185 1107 16-17 0.5374594268185362 1107 18-19 0.3416294920876908 1107 20-21 0.41022411026062144 1107 22-23 0.4058849143956351 1107 24-25 0.46620110023471284 1107 26-27 0.28621126514302375 1107 28-29 0.36302084757495123 1107 30-31 0.38493325344007445 1107 32-33 0.5821128182281612 1107 34-35 0.4876995616066466 1107 36-37 0.5790103327258009 1107 38-39 0.5137785747916226 1107 40-41 0.5788690620876054 1107 42-43 0.8152945929866249 1107 44-45 0.6247683536860791 1107 46-47 0.35386102684407916 1107 48-49 0.4804937771815574 1107 50-51 0.33942737972406434 1107 52-53 0.4082873363677919 1107 54-55 0.5353232690667369 1107 56-57 0.6451883482208984 1107 58-59 0.616181120844665 1107 60-61 0.6224307567074732 1107 62-63 0.8774474393730785 1107 64-65 0.7304355771432114 1107 66-67 0.5065740289996974 1107 68-69 0.6596752655879072 1107 70-71 0.9472102936346083 1107 72-73 1.0276111571391695 1107 74-75 0.8986554066222574 1107 76-77 0.8657382958268123 1107 78-79 1.1696191874129838 1107 80-81 1.1567453464483641 1107 82-83 0.8784501144322618 1107 84-85 1.1057923506184686 1107 86-87 1.3292786888867845 1107 88-89 1.121256494626909 1107 90-91 1.3431813397016334 1107 92-93 1.2931090619643442 1107 94-95 1.4313147754338829 1107 96-97 1.3643480621163917 1107 98-99 1.7304796706144394 1107 100-101 1.5704825959142141 1108 1 0.05952019628254135 1108 2 -0.1105738919497874 1108 3 -0.12911393115756553 1108 4 -0.06584329740889672 1108 5 -0.22202939248298037 1108 6 -0.2500238780081361 1108 7 -0.07861499550872253 1108 8 0.0697412670617652 1108 9 0.004305124014770456 1108 10-11 0.15000892628980012 1108 12-13 0.05188779679027533 1108 14-15 0.14084933256441445 1108 16-17 0.19792527493890333 1108 18-19 0.1923282468819636 1108 20-21 0.08544222120598732 1108 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-2.0167160665895203 2316 56-57 -2.1704642730779042 2316 58-59 -2.0145310677249526 2316 60-61 -1.9918715498875983 2316 62-63 -1.9740202538161995 2316 64-65 -1.9862971726865695 2316 66-67 -2.078176472400532 2316 68-69 -2.1849176220768776 2316 70-71 -2.6740238132876435 2316 72-73 -2.4056694945116774 2316 74-75 -2.5214709986077075 2316 76-77 -2.8391780450452373 2316 78-79 -2.9269076725430843 2316 80-81 -3.412273049009304 2316 82-83 -3.3102704600753476 2316 84-85 -3.365619364432167 2316 86-87 -3.1751060599201786 2316 88-89 -3.63190132552214 2316 90-91 -3.7582640176865887 2316 92-93 -3.7570402028027026 2316 94-95 -3.9542327289639587 2316 96-97 -4.414486686844608 2316 98-99 -4.395744562343342 2316 100-101 -4.199517188429775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4983.0 3 989.0 4 812.0 5 767.0 6 858.0 7 1008.0 8 1241.0 9 1513.0 10 1956.0 11 2649.0 12 3615.0 13 4544.0 14 5507.0 15 6668.0 16 7826.0 17 9490.0 18 8658.0 19 7579.0 20 7846.0 21 8420.0 22 9289.0 23 10047.0 24 11205.0 25 12845.0 26 14861.0 27 16891.0 28 19355.0 29 22972.0 30 26755.0 31 31513.0 32 37543.0 33 45148.0 34 56258.0 35 70927.0 36 90045.0 37 107226.0 38 63999.0 39 3638.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25807742119217 21.036757775683316 15.090555265495425 22.614609537629086 2 15.17976773072511 22.162642792661323 40.231862023091175 22.42572745352239 3 18.704171582410034 25.329122877518905 31.600276412984897 24.366429127086164 4 12.258693417942524 18.77381839706855 38.872031313003774 30.095456871985153 5 12.713678406019529 38.16629768970623 35.72941991389029 13.390603990383962 6 29.50265624618258 37.90206771866865 18.593723278054817 14.001552757093954 7 25.6707173987057 33.89104936973793 22.756713277891794 17.681519953664584 8 23.689250593421498 35.60610376398779 21.675957951848083 19.028687690742625 9 24.45226317409981 17.426257458349216 22.893249473388664 35.22822989416231 10-11 23.03202566937787 27.647561612612186 29.498212505002954 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20.29601851167189 28.696144147908853 30.75448901937932 20.253348321039933 38-39 19.993733009945792 28.95105236599222 30.90046609042914 20.154748533632848 40-41 20.3423411079963 28.83426178459343 30.620238903520878 20.203158203889387 42-43 20.076642125360745 28.870546024274528 30.329934175944544 20.722877674420182 44-45 20.53346153298485 28.628957616137424 30.28465882777331 20.55292202310442 46-47 20.546784991859322 28.820846101527568 29.98427469310562 20.64809421350749 48-49 20.263268706175282 28.630895592320687 30.291768123371355 20.81406757813268 50-51 20.15538567734079 28.81137686790319 30.182833801877155 20.850403652878864 52-53 20.297297242317867 29.09416017461907 29.750270045221132 20.85827253784193 54-55 20.41811326895953 28.546571552565908 29.806336118042747 21.22897906043181 56-57 20.648621890395447 28.657152641447382 29.457944685742188 21.23628078241499 58-59 20.72470166344205 28.511392635120338 29.509769814450497 21.25413588698712 60-61 20.523471653480293 28.385990659897374 29.891380276188162 21.199157410434168 62-63 20.324792537732435 28.307563574521637 29.912200639305198 21.455443248440726 64-65 20.327618964958415 28.512058216851262 29.59114758856005 21.56917522963027 66-67 20.353094185120842 28.353723451144408 29.359735382787676 21.93344698094707 68-69 20.361290147587837 28.156694683379502 29.635556700786253 21.846458468246407 70-71 20.49708018800741 27.94894228877992 29.59454968428049 21.959427838932182 72-73 20.42778993287043 27.488331337125 30.058896058358915 22.024982671645656 74-75 20.43024025523049 27.922684803368348 29.50980445351159 22.137270487889573 76-77 20.38998242149352 27.63135050673843 29.61740977451768 22.361257297250376 78-79 20.38571826686475 27.59459373328171 29.67737473445678 22.34231326539676 80-81 20.521857760467803 27.442623039744834 29.645621667362803 22.38989753242456 82-83 20.562790082689457 27.450527022399722 29.726504361623142 22.26017853328768 84-85 20.617487198403055 27.42375998090609 29.570129426314878 22.388623394375976 86-87 20.525939262016895 27.335482930368656 29.56279094999305 22.57578685762141 88-89 20.83170261703682 27.125261395061116 29.390741782037725 22.652294205864337 90-91 21.004249996947816 26.872243708091816 29.35427990835308 22.769226386607286 92-93 21.03132456251009 26.81937611313901 29.47809125399984 22.671208070351064 94-95 20.999717812285475 26.690887778829953 29.398465332967938 22.910929075916638 96-97 20.973575465529265 26.487685825064645 29.506198464831957 23.03254024457414 98-99 21.302702156475906 26.466050272331636 29.0650523672947 23.166195203897757 100-101 21.69822138718636 25.816992798833482 29.50144152249745 22.9833442914827 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 664.0 1 392.5 2 148.0 3 165.0 4 127.5 5 123.5 6 198.5 7 360.0 8 514.0 9 549.5 10 465.0 11 387.0 12 386.5 13 408.5 14 483.5 15 593.0 16 692.0 17 767.0 18 887.0 19 1052.0 20 1259.0 21 1585.0 22 1974.5 23 2440.0 24 3062.5 25 3882.0 26 4995.0 27 6285.5 28 7814.5 29 9873.5 30 12010.0 31 13997.0 32 16119.5 33 18383.0 34 20665.5 35 22663.5 36 24412.5 37 26078.0 38 27112.5 39 27666.5 40 28430.5 41 29201.0 42 29749.0 43 30034.5 44 30219.0 45 30081.5 46 30022.0 47 30913.5 48 33020.0 49 32477.0 50 28409.5 51 24906.5 52 21887.5 53 18731.0 54 16113.0 55 13894.5 56 11449.5 57 9067.5 58 7163.5 59 5581.5 60 4121.0 61 2955.0 62 2106.5 63 1470.0 64 1046.5 65 736.5 66 542.0 67 427.0 68 327.0 69 235.5 70 181.5 71 147.0 72 115.0 73 91.5 74 67.5 75 49.5 76 41.5 77 33.5 78 25.5 79 18.0 80 15.0 81 12.0 82 6.0 83 3.5 84 2.5 85 2.5 86 1.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007051354199013623 2 0.1087535782232486 3 0.11770337393738127 4 0.11987302138323161 5 0.15960469023536605 6 0.0939728549983931 7 0.029019034588248373 8 0.026713784177032383 9 0.02522215155801027 10-11 0.05180033276967701 12-13 0.05600402469601206 14-15 0.028069813830688847 16-17 0.03213790279165825 18-19 0.03735861695823564 20-21 0.06013991513966427 22-23 0.033154925031900595 24-25 0.04935947939309537 26-27 0.05742785583235134 28-29 0.05363097280211323 30-31 0.06888630640574848 32-33 0.05051210459870336 34-35 0.06380119520453673 36-37 0.05403778169821018 38-39 0.0340363443067773 40-41 0.0389858525426234 42-43 0.03451095468555706 44-45 0.007525964577793387 46-47 0.013628098019247484 48-49 0.00861078830071856 50-51 0.007593766060476211 52-53 0.009560009058278087 54-55 0.011797457986811255 56-57 0.015526539534366538 58-59 0.020408246287529817 60-61 0.026578181211666736 62-63 0.011390649090714315 64-65 0.008949795714132677 66-67 0.01084823722925173 68-69 0.012136465400225373 70-71 0.032815917618486484 72-73 0.02929024051897967 74-75 0.031324284999464366 76-77 0.024815342661913327 78-79 0.0058987289934056275 80-81 0.005830927510722805 82-83 0.006712346785599507 84-85 0.005288515649260218 86-87 0.013492495053881838 88-89 0.040206279230914216 90-91 0.03661280064872459 92-93 0.031188682034098723 94-95 0.04732543491261067 96-97 0.04461337560529774 98-99 0.027459600486543438 100-101 0.07492063836451975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 737447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.68070376578356 #Duplication Level Percentage of deduplicated Percentage of total 1 78.31791624478917 53.78929605161814 2 13.617911864085997 18.70575541291679 3 4.339005530417491 8.940178604181007 4 1.744988846283723 4.793882481048352 5 0.8336269833094819 2.8627043945921162 6 0.4226396502609315 1.7416313171547242 7 0.229973551550666 1.1056321757611514 8 0.13782011826652185 0.7572466172502594 9 0.0892114444787871 0.5514394311688702 >10 0.2461742757446388 2.687533309170973 >50 0.010267222930989224 0.5036427977315755 >100 0.0073052436224465694 1.0124083102656374 >500 0.001974390161986715 0.8152422650709238 >1k 0.0011846340971920291 1.7334068320694642 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3276 0.4442353145378583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3095 0.4196911778066763 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1988 0.26957869514690547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1946 0.2638833706015483 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1351 0.18319960620898856 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1127 0.15282454196708373 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 857 0.11621174131835915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 803 0.10888918118861424 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3560296536564663E-4 0.0 4 0.0 0.0 0.0 1.3560296536564663E-4 0.0 5 0.0 0.0 0.0 1.3560296536564663E-4 0.0 6 0.0 0.0 0.0 1.3560296536564663E-4 0.0 7 0.0 0.0 0.0 1.3560296536564663E-4 0.0 8 0.0 0.0 0.0 1.3560296536564663E-4 0.0 9 0.0 0.0 0.0 1.3560296536564663E-4 0.0 10-11 0.0 0.0 0.0 1.3560296536564663E-4 0.0 12-13 0.0 0.0 0.0 1.3560296536564663E-4 0.0 14-15 0.0 0.0 0.0 2.0340444804846993E-4 0.0 16-17 0.0 0.0 0.0 2.7120593073129325E-4 0.0 18-19 0.0 0.0 0.0 2.7120593073129325E-4 0.0 20-21 0.0 0.0 0.0 8.81419274876703E-4 0.0 22-23 0.0 0.0 0.0 0.002373051893898816 0.0 24-25 0.0 0.0 0.0 0.005220714166577395 0.0 26-27 0.0 0.0 0.0 0.010577031298520435 0.0 28-29 0.0 0.0 0.0 0.02637477676361827 0.0 30-31 0.0 0.0 0.0 0.06997113012867365 0.0 32-33 0.0 0.0 0.0 0.1524177330709868 0.0 34-35 0.0 0.0 0.0 0.2966992882200348 0.0 36-37 0.0 0.0 0.0 0.5110875764631221 0.0 38-39 0.0 0.0 0.0 0.7488673762317835 0.0 40-41 0.0 0.0 0.0 0.9758667402538759 0.0 42-43 0.0 0.0 0.0 1.2057815680313295 0.0 44-45 0.0 0.0 0.0 1.5151597335130527 0.0 46-47 0.0 0.0 0.0 1.8726769516995798 0.0 48-49 0.0 0.0 0.0 2.204565209432 0.0 50-51 0.0 0.0 0.0 2.511909330433238 0.0 52-53 0.0 0.0 0.0 2.8206094810881326 0.0 54-55 0.0 0.0 0.0 3.2057897042092516 0.0 56-57 0.0 0.0 0.0 3.6602630426322165 0.0 58-59 0.0 0.0 0.0 4.103616937895198 0.0 60-61 0.0 0.0 0.0 4.542156927887699 0.0 62-63 0.0 0.0 0.0 4.973103151819724 0.0 64-65 0.0 0.0 0.0 5.462901062720439 0.0 66-67 0.0 0.0 0.0 6.023348118576657 0.0 68-69 0.0 0.0 0.0 6.6539019075269135 0.0 70-71 0.0 0.0 0.0 7.284387894994488 0.0 72-73 0.0 0.0 0.0 7.899008335514281 0.0 74-75 0.0 0.0 0.0 8.545020862516221 0.0 76-77 0.0 0.0 0.0 9.250156282417585 0.0 78-79 0.0 0.0 0.0 10.038280717122722 0.0 80-81 0.0 0.0 0.0 10.842338500258325 0.0 82-83 0.0 0.0 0.0 11.632903788340043 0.0 84-85 0.0 0.0 0.0 12.437910792233204 0.0 86-87 0.0 0.0 0.0 13.293158694794338 0.0 88-89 0.0 0.0 0.0 14.200817143469294 6.780148268282331E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 60 0.003929752 31.699991 5 CGCCGGT 30 0.0039486946 31.670952 94-95 TGTAGCG 70 0.008346709 27.171423 5 GTATAGA 290 0.0 24.594824 1 GTATAGC 455 0.0 24.036259 1 TAGGACG 145 2.3139775E-5 22.955168 4 GTATAGT 220 5.5701094E-8 21.613632 1 GAGTACT 2935 0.0 21.049887 12-13 TAGCCCT 875 0.0 20.650282 4 TCGCCGG 70 4.8482034E-4 20.3599 94-95 AGTACTT 2840 0.0 20.24797 12-13 ATAGCCC 450 0.0 20.07666 3 GTACTTT 3045 0.0 19.969185 14-15 TATAGCC 575 0.0 19.019997 2 GTACATG 2315 0.0 18.691357 1 GTGTAGC 575 0.0 18.193039 1 CGGGTCC 80 0.0011898795 17.815516 34-35 GTAAGCG 95 1.8641973E-4 17.502369 94-95 GTCCTAC 245 3.7204845E-6 17.467342 1 AGAGTAC 4430 0.0 17.433271 10-11 >>END_MODULE