FastQCFastQC Report
Thu 26 May 2016
SRR938563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737447
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.41630110367253514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30620.41521627994960997No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20210.2740535930039718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.2641545765322796No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17110.23201667374062135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13440.18225038545142905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.1503836885905021No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT9740.1320772882661398RNA PCR Primer, Index 23 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9530.12922962599346124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTA352.7932483E-454.2622347
GGCGAGC500.001618877337.9835668
ATGCCGC753.3267497E-828.50990394-95
AGGACGT1401.7777782E-523.7515
GGACGTG1050.002201007222.6092646
GTATTAC1302.92717E-421.9373931
GTAGGAC2657.912604E-1021.510343
CATTCCG1553.9170234E-521.4423359
TAGGACG1353.793082E-421.1120014
ACCGTGT1150.00373137220.643248
GAGTACT28450.020.52714512-13
CGTCAGA1200.004743954619.8045922
AGTACTT27350.019.6167412-13
TCCGGTG857.98229E-519.55036412-13
GTACTTT29300.019.36448914-15
GTACATG23250.017.9902361
GGTGTTC1851.5254454E-417.9651998
CTCCGAC2002.1827873E-1117.8186994-95
CGGTGTT800.001194693517.80479414-15
TACATGG23300.017.7476332
Produced by FastQC (version 0.11.5)