FastQCFastQC Report
Thu 26 May 2016
SRR938562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735974
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.46170109270164433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.42202577808455194No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20230.27487384065197956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.26427564017207134No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13060.1774519208558998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11200.15217928894227242No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8750.11889006948615033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.10435151241755822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG452.4000954E-531.69232494-95
ATACCGA700.0083773827.1509956
GTACATG21750.021.8828261
ATAGTAC1553.88882E-521.4624233
GTATAGC4500.021.1533991
GAGTACT28700.021.1010712-13
TACATGG21800.020.9279732
GTACTTT30150.019.6143114-15
ATAGCCC4150.019.4676363
ACATGGG22950.019.4651413
AGTACTT28500.018.74924912-13
CTCGGCG901.2259834E-418.48718894-95
GGTATCA49950.018.0089741
AGAGTAC43050.017.59921310-11
GTACAGC1952.2687105E-417.0563956
GTATCAA60050.016.9614681
TAGCCCT8350.016.505324
TATAGCC5500.016.4173622
CTAACAC1750.002161451816.293923
TCTACAC2657.890971E-616.1402053
Produced by FastQC (version 0.11.5)