FastQCFastQC Report
Thu 26 May 2016
SRR938562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735974
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31540.4285477476106493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.40680784919032464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.2732433482704552No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19720.2679442480305011No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17820.2421281186563656No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14040.19076760863834863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10650.14470619886028582No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT10390.14117346536698308RNA PCR Primer, Index 23 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9650.13111876234758293No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.10027528146374735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG459.6257287E-442.2196246
AGCGGGG600.00393024331.6991792
CTCCGAC1306.2573235E-1021.92172894-95
GTATAGG2257.065864E-821.1327861
GAGTACT29600.020.61950912-13
GTATAGC4500.020.0761471
GTACTTT30150.019.84952514-15
GCATGCG1200.004763595819.790459
AGTACTT28250.019.33509412-13
GGGAGCG1300.00756329218.2681068
GTAGGAC2101.9427402E-518.1076583
CCTATAC2152.378458E-517.6865483
GTATCAA58500.017.5564671
GGTATCA47900.017.4709451
AGAGTAC44050.017.30597310-11
TAGTAGA2555.464961E-616.7762134
ACTTTTT37450.016.36081116-17
ATAGCCC4106.039045E-1016.2306443
GTGTAGC4451.10958354E-1016.0276741
TTAGAGC3001.4538127E-615.84423
Produced by FastQC (version 0.11.5)