FastQCFastQC Report
Thu 26 May 2016
SRR938561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734510
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35740.4865828919960246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.42627057494111725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.28290969489863993No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20420.278008468230521No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12800.17426583708867135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11040.15030428448897906No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9050.12321139262909968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.10592095410545807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC1300.025.5974294-95
CGCCGCA601.7129035E-423.74637692-93
AGTACTT29700.020.87513412-13
CGCCGTA1601.8189894E-1120.77807692-93
GTACATG22300.020.7302761
GACGCTC1058.5450483E-720.37345994-95
GCCGTAT1753.6379788E-1220.37345994-95
TACATGG22350.019.7768972
ACATGGG22100.019.1403753
GAGTACT32000.018.78087212-13
GTAAGCG650.00752923618.2838794-95
TCGCCGT1951.4551915E-1118.26644192-93
GTACTTT31900.018.02062614-15
TAGCCCT7450.017.8630034
GTAGCCC4800.017.8231323
GTATTAG1901.8176816E-417.5583061
GTACTAG3007.382732E-817.4746971
AGAGTAC46300.017.18735310-11
GATCAAT1952.2168514E-417.1080931
TCTCGGG2700.016.72635794-95
Produced by FastQC (version 0.11.5)