##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938561_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 734510 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.180792637268382 31.0 31.0 34.0 28.0 34.0 2 31.330910402853604 31.0 31.0 34.0 28.0 34.0 3 31.37716981388953 33.0 31.0 34.0 28.0 34.0 4 35.0281929449565 37.0 35.0 37.0 32.0 37.0 5 35.04489795918367 37.0 35.0 37.0 32.0 37.0 6 35.0636546813522 37.0 35.0 37.0 32.0 37.0 7 34.996258730310004 37.0 35.0 37.0 32.0 37.0 8 34.97623313501518 37.0 35.0 37.0 32.0 37.0 9 36.494744795850295 39.0 37.0 39.0 32.0 39.0 10-11 36.37971368667547 39.0 37.0 39.0 32.0 39.0 12-13 36.26236538644811 39.0 35.0 39.0 32.0 39.0 14-15 37.289028740248604 40.0 37.0 41.0 32.0 41.0 16-17 37.14195313882725 39.0 36.0 41.0 31.0 41.0 18-19 37.07062599556167 39.0 36.0 41.0 31.0 41.0 20-21 37.09083470613062 39.0 36.5 41.0 31.0 41.0 22-23 37.167532776953344 39.0 37.0 41.0 31.0 41.0 24-25 37.11369416345591 39.0 36.0 41.0 31.0 41.0 26-27 36.95276102435638 39.0 36.0 41.0 31.0 41.0 28-29 36.796520129065634 39.0 36.0 41.0 30.0 41.0 30-31 36.57565928305945 39.0 36.0 40.5 30.0 41.0 32-33 36.28981906304884 39.0 35.0 40.0 30.0 41.0 34-35 36.06895889776858 38.5 35.0 40.0 30.0 41.0 36-37 35.826612299356036 38.0 35.0 40.0 29.0 41.0 38-39 35.55853085730624 38.0 35.0 40.0 27.5 41.0 40-41 35.48035356904603 38.0 35.0 40.0 27.0 41.0 42-43 35.28784427713714 38.0 35.0 40.0 26.5 41.0 44-45 34.984015193802676 38.0 34.0 40.0 25.5 41.0 46-47 34.60080121441505 38.0 34.0 40.0 24.0 41.0 48-49 34.25467794856435 38.0 33.0 40.0 23.0 41.0 50-51 33.521316932376685 37.5 33.0 39.5 21.5 40.5 52-53 33.5794461613865 38.0 33.0 39.5 22.0 40.5 54-55 33.721374794080404 38.0 33.0 40.0 19.5 41.0 56-57 33.45436617609018 38.0 33.0 40.0 17.5 41.0 58-59 33.10865679160257 38.0 32.5 40.0 13.5 41.0 60-61 32.69854052361438 37.0 31.5 40.0 8.5 41.0 62-63 32.21548447264163 37.0 31.0 39.5 8.0 41.0 64-65 31.814234659841254 36.0 31.0 39.0 4.5 41.0 66-67 31.327345441178473 36.0 30.0 39.0 2.0 40.0 68-69 30.75691345250575 35.5 29.0 39.0 2.0 40.0 70-71 30.171974513621326 35.0 28.5 38.0 2.0 40.0 72-73 29.58129977808335 34.5 28.0 37.5 2.0 39.5 74-75 28.916634218730856 34.0 26.5 37.0 2.0 39.0 76-77 28.193702604457393 34.0 26.0 36.0 2.0 39.0 78-79 27.421286980435937 33.0 25.5 36.0 2.0 38.5 80-81 26.67552994513349 32.5 23.0 35.0 2.0 37.0 82-83 25.952202829096947 32.0 21.0 35.0 2.0 37.0 84-85 25.17634205116336 31.5 18.5 35.0 2.0 36.0 86-87 24.43003907366816 31.0 11.0 34.5 2.0 36.0 88-89 23.64950783515541 31.0 4.5 34.0 2.0 35.0 90-91 22.813491987855848 30.0 2.0 34.0 2.0 35.0 92-93 21.941204340308506 29.0 2.0 34.0 2.0 35.0 94-95 21.015720684537992 29.0 2.0 34.0 2.0 35.0 96-97 19.904899865216265 28.0 2.0 34.0 2.0 35.0 98-99 18.643343181168397 25.5 2.0 34.0 2.0 35.0 100-101 15.625047310451865 11.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4969374809643732 1101 2 0.6833543203586565 1101 3 0.6247093203363896 1101 4 0.4875688083141938 1101 5 0.46864250776029337 1101 6 0.3860790558738003 1101 7 0.36796079451149666 1101 8 0.3108091686403043 1101 9 0.5946920574716543 1101 10-11 0.526213520148616 1101 12-13 0.6656375730404207 1101 14-15 0.6627605141725184 1101 16-17 0.7722219983027259 1101 18-19 0.8704628361222859 1101 20-21 1.0684878863247107 1101 22-23 0.8862365805421533 1101 24-25 0.9456457497000557 1101 26-27 0.8235777142637275 1101 28-29 0.8319473682506029 1101 30-31 1.0667519293299108 1101 32-33 1.085087735451289 1101 34-35 1.1285509688237951 1101 36-37 1.0645131893590332 1101 38-39 1.169970200354598 1101 40-41 1.0821724276289615 1101 42-43 1.3252389880422442 1101 44-45 0.9017317740484074 1101 46-47 1.046737443262792 1101 48-49 1.0900103736771243 1101 50-51 1.1281598000322717 1101 52-53 1.4465193436035477 1101 54-55 1.486274320442547 1101 56-57 1.5700363253304772 1101 58-59 1.7180916599467508 1101 60-61 1.8044363478614613 1101 62-63 1.8595002753628336 1101 64-65 1.876815713770739 1101 66-67 1.6467929861029766 1101 68-69 1.7548560764526435 1101 70-71 2.031551979331276 1101 72-73 2.027694345691277 1101 74-75 2.3038325976658065 1101 76-77 2.7805840086766054 1101 78-79 2.6471052446502235 1101 80-81 3.0777891501337926 1101 82-83 3.154913880650561 1101 84-85 3.7262859280019107 1101 86-87 3.791037677587944 1101 88-89 3.8221151640156386 1101 90-91 4.0987325123640765 1101 92-93 4.468241001839367 1101 94-95 4.714371038816733 1101 96-97 4.9424220282996 1101 98-99 5.4683144458297726 1101 100-101 5.665076192431577 1102 1 0.30062916938756246 1102 2 0.1530042691047555 1102 3 0.12726028688881996 1102 4 0.0854004281017211 1102 5 0.1016809845459079 1102 6 0.12035395975807006 1102 7 0.3294582060134843 1102 8 0.14182845277458256 1102 9 0.4329431851747927 1102 10-11 0.3360439483122235 1102 12-13 0.30380730438552916 1102 14-15 0.7006086478032856 1102 16-17 0.7726371901002196 1102 18-19 0.5370790698869996 1102 20-21 0.7567245878721778 1102 22-23 0.3680103643468513 1102 24-25 0.45294327332728557 1102 26-27 0.4598192667526533 1102 28-29 0.5046014759518229 1102 30-31 0.4899742868217771 1102 32-33 0.5737884196967542 1102 34-35 0.6237058916614515 1102 36-37 0.7989757505529056 1102 38-39 0.8858124650030135 1102 40-41 0.8836145740532686 1102 42-43 1.103728299785601 1102 44-45 0.9922424130450054 1102 46-47 0.9770286170358062 1102 48-49 1.218147476107525 1102 50-51 1.3546129126806008 1102 52-53 1.3433488733577832 1102 54-55 1.4091014247260603 1102 56-57 1.6108107401328908 1102 58-59 1.4005438249256699 1102 60-61 1.4881434710983612 1102 62-63 1.7109976634076531 1102 64-65 1.7678947198437953 1102 66-67 1.8969085126342087 1102 68-69 2.195523526588577 1102 70-71 2.272328036135143 1102 72-73 2.043224160919202 1102 74-75 1.9917209603323194 1102 76-77 2.1842911788278307 1102 78-79 2.959598060893839 1102 80-81 3.135927730365502 1102 82-83 3.584095530580541 1102 84-85 3.7394817554416306 1102 86-87 3.662710572131573 1102 88-89 4.118532785975294 1102 90-91 4.511751003646221 1102 92-93 4.356701015585259 1102 94-95 4.951598026752713 1102 96-97 5.0380545389902025 1102 98-99 5.235606480480694 1102 100-101 5.63134361817206 1103 1 0.274033109398232 1103 2 0.3975194080457847 1103 3 0.3222651279249966 1103 4 0.2831979213012161 1103 5 0.22461037128212524 1103 6 0.20240128994308293 1103 7 0.44138164519097245 1103 8 0.28566321207299694 1103 9 0.508917814193353 1103 10-11 0.45346048160340047 1103 12-13 0.4768957537390719 1103 14-15 0.4883868427225053 1103 16-17 0.5026522730564835 1103 18-19 0.6543441149712095 1103 20-21 0.6001648191880875 1103 22-23 0.6932456650509451 1103 24-25 0.927078308597693 1103 26-27 0.6981365620953781 1103 28-29 0.8599434111807369 1103 30-31 0.8606560496175888 1103 32-33 0.6394091590909383 1103 34-35 0.9358769811251193 1103 36-37 0.8950620856579903 1103 38-39 0.7560364699560154 1103 40-41 0.7854963210416201 1103 42-43 0.9427128018833812 1103 44-45 0.9538697915745118 1103 46-47 1.0894196937217728 1103 48-49 0.9590071387498682 1103 50-51 1.1015288103799605 1103 52-53 1.2034561006625424 1103 54-55 1.2377089846462326 1103 56-57 1.8159949300332272 1103 58-59 1.7970213947913791 1103 60-61 1.7157248630773907 1103 62-63 1.8035964765304868 1103 64-65 1.8560410969703938 1103 66-67 2.1078097357368186 1103 68-69 2.1639645812099566 1103 70-71 2.2014150891626016 1103 72-73 2.5581918544124598 1103 74-75 2.5485981956066794 1103 76-77 2.4075815026832714 1103 78-79 2.7284764553823067 1103 80-81 2.963234467638845 1103 82-83 2.985924586018637 1103 84-85 3.439600970074075 1103 86-87 3.3567175669348686 1103 88-89 3.7866781092708877 1103 90-91 4.132253285848993 1103 92-93 4.044671534662896 1103 94-95 4.431575237657228 1103 96-97 4.803978913442705 1103 98-99 5.034940591014394 1103 100-101 5.23203058425846 1104 1 0.45126886166965363 1104 2 0.5758710548137067 1104 3 0.5319400567403996 1104 4 0.534187711484229 1104 5 0.2905016765698605 1104 6 0.44497245909992245 1104 7 0.5870738188999169 1104 8 0.4999046611711151 1104 9 0.588798977606011 1104 10-11 0.6230115914243015 1104 12-13 0.5870532760415159 1104 14-15 0.5719523109349467 1104 16-17 0.796381645993776 1104 18-19 0.9390526544464848 1104 20-21 0.8153945143347627 1104 22-23 0.7311181360160717 1104 24-25 0.846752434463184 1104 26-27 0.9058267634299213 1104 28-29 0.7799006283622205 1104 30-31 0.9380511565643417 1104 32-33 0.9214772286552204 1104 34-35 0.934662262023771 1104 36-37 1.0412257350859377 1104 38-39 1.1024043569006636 1104 40-41 1.0984944330798925 1104 42-43 0.9134185125043501 1104 44-45 1.0507096689496578 1104 46-47 0.9040298821267712 1104 48-49 0.8374724338133746 1104 50-51 0.8726335253430406 1104 52-53 0.7062273072903835 1104 54-55 0.909287028289647 1104 56-57 1.0410504674023144 1104 58-59 1.352024493354513 1104 60-61 1.2264349796286567 1104 62-63 1.4318296367148662 1104 64-65 1.502011214880497 1104 66-67 1.5655272167799943 1104 68-69 1.8407401814150184 1104 70-71 1.9867577531857137 1104 72-73 2.066941949634078 1104 74-75 1.8883077747991557 1104 76-77 2.1262772649606845 1104 78-79 2.214410419957801 1104 80-81 2.2013348528489196 1104 82-83 2.813968744146237 1104 84-85 3.030099601970953 1104 86-87 2.962241656461728 1104 88-89 3.3598515526289496 1104 90-91 3.659354904307783 1104 92-93 3.4336678266813685 1104 94-95 4.043226452685161 1104 96-97 4.217236645953324 1104 98-99 4.500827572725665 1104 100-101 4.9156178762378175 1105 1 0.5990995608886429 1105 2 0.5476305560743917 1105 3 0.6766651658972798 1105 4 0.7151978738358693 1105 5 0.5408245349426579 1105 6 0.5006616848547978 1105 7 0.615241290150152 1105 8 0.6123593072568454 1105 9 0.7707604190917721 1105 10-11 0.9396193876852976 1105 12-13 0.7428391671306258 1105 14-15 0.7781598396847187 1105 16-17 0.8848986498680773 1105 18-19 0.9291170723789648 1105 20-21 1.0759258469711384 1105 22-23 0.8676353397550756 1105 24-25 1.0569880057687584 1105 26-27 1.0321047444888976 1105 28-29 1.0277136472417467 1105 30-31 1.0597524280113646 1105 32-33 1.08239041818846 1105 34-35 1.110327965240053 1105 36-37 1.372137310327318 1105 38-39 1.2846521430146822 1105 40-41 1.2291915381164813 1105 42-43 1.3214259766824483 1105 44-45 1.4296051089594926 1105 46-47 1.258987679925177 1105 48-49 1.3899292322882602 1105 50-51 1.3689120060637805 1105 52-53 1.3198965158261373 1105 54-55 1.3657645665714284 1105 56-57 1.5256483934216263 1105 58-59 1.709859189867636 1105 60-61 2.0382133527299473 1105 62-63 1.9825626020921518 1105 64-65 2.2319815042231 1105 66-67 2.448448777569613 1105 68-69 2.1947755267197415 1105 70-71 2.4772728275968774 1105 72-73 2.553649024844706 1105 74-75 2.4092339939738707 1105 76-77 2.538612568755042 1105 78-79 2.8179268857348134 1105 80-81 3.243534171771497 1105 82-83 3.243155141046799 1105 84-85 3.5614178250199657 1105 86-87 3.8388963056222956 1105 88-89 4.107190853717441 1105 90-91 4.398175933149798 1105 92-93 4.467429587950662 1105 94-95 5.086326123677104 1105 96-97 5.1175310620408965 1105 98-99 5.316953003203491 1105 100-101 5.300645703554707 1106 1 0.301612034160204 1106 2 0.34350695189287705 1106 3 0.30695145717786687 1106 4 0.22071432667502222 1106 5 0.36233795855871875 1106 6 0.07346698302538357 1106 7 0.3522557235072412 1106 8 0.40527330107960324 1106 9 0.4144109299019405 1106 10-11 0.37946926295258265 1106 12-13 0.16112810155685509 1106 14-15 0.34707095277571653 1106 16-17 0.4275156610346187 1106 18-19 0.6950204936191682 1106 20-21 0.6146820836069153 1106 22-23 0.3954254918101938 1106 24-25 0.37171166340715445 1106 26-27 0.2044660535884475 1106 28-29 0.30023978171549004 1106 30-31 0.4480758124257136 1106 32-33 0.7356520302103178 1106 34-35 0.7085104313840276 1106 36-37 0.9411820656136101 1106 38-39 0.734157666892088 1106 40-41 0.682903078862509 1106 42-43 0.43899027342752106 1106 44-45 0.7838534458566144 1106 46-47 1.238622094275378 1106 48-49 1.0335203465671796 1106 50-51 0.7699165498984186 1106 52-53 0.9990958505849861 1106 54-55 1.1361895487524691 1106 56-57 1.1894677388718549 1106 58-59 0.7825011475773067 1106 60-61 1.5354477017439692 1106 62-63 1.8910102737481296 1106 64-65 2.22209569405441 1106 66-67 2.363942362744236 1106 68-69 1.4694028134861874 1106 70-71 1.9613656940734785 1106 72-73 2.147562365305621 1106 74-75 2.361293568694709 1106 76-77 2.5326023822499337 1106 78-79 2.3447666932900546 1106 80-81 2.7516677842685233 1106 82-83 2.9658921332064594 1106 84-85 2.985995367191503 1106 86-87 3.302567902201055 1106 88-89 3.4836052336176557 1106 90-91 3.7681643508262077 1106 92-93 4.095280621450307 1106 94-95 4.212462082337119 1106 96-97 4.5493883915728475 1106 98-99 4.756914246966858 1106 100-101 4.674027148078345 1107 1 0.4700861829622198 1107 2 0.4560962751379378 1107 3 0.5573507846263759 1107 4 0.4941485427321908 1107 5 0.3948936542291648 1107 6 0.40934340168868744 1107 7 0.41595896016129785 1107 8 0.16935484794803557 1107 9 0.3788707869847485 1107 10-11 0.6208101624902014 1107 12-13 0.6940410902075485 1107 14-15 0.7078107594171641 1107 16-17 0.843036888441091 1107 18-19 0.913021754653208 1107 20-21 0.7860986451874155 1107 22-23 0.6551144367450448 1107 24-25 0.757430452814468 1107 26-27 0.7502415895279171 1107 28-29 0.9541489872640412 1107 30-31 1.1125987212109152 1107 32-33 0.9861564438294863 1107 34-35 1.0256289685845346 1107 36-37 1.0491265801155052 1107 38-39 1.0693720426802287 1107 40-41 0.9211334277485932 1107 42-43 0.8058776210525735 1107 44-45 0.9963902260163522 1107 46-47 0.9179411358895067 1107 48-49 1.06531942180888 1107 50-51 1.3286702279143938 1107 52-53 1.262956716495033 1107 54-55 1.1318389812871459 1107 56-57 1.345426124748272 1107 58-59 1.499714262331402 1107 60-61 1.6379172267544746 1107 62-63 1.7712540736640534 1107 64-65 1.5891616881695825 1107 66-67 1.9086355111259117 1107 68-69 2.186699997176813 1107 70-71 1.8639192880205115 1107 72-73 2.2104564384455934 1107 74-75 2.4124600251890307 1107 76-77 2.5615536222667465 1107 78-79 2.522908769792785 1107 80-81 2.7364933412252306 1107 82-83 2.9239522424960747 1107 84-85 3.2650096589107136 1107 86-87 3.36391720863233 1107 88-89 3.8086157422347213 1107 90-91 4.356321139392829 1107 92-93 4.6783827652081875 1107 94-95 4.875240560689367 1107 96-97 4.879947450000763 1107 98-99 5.191224881687329 1107 100-101 5.544918278805547 1108 1 0.4607829207793763 1108 2 0.3616742050614157 1108 3 0.49395972542379596 1108 4 0.3928597791397621 1108 5 0.3491559713187087 1108 6 0.31996227670546773 1108 7 0.24029108527876275 1108 8 0.38998849004764935 1108 9 0.2523504486843109 1108 10-11 0.5413330514685839 1108 12-13 0.6403358948793141 1108 14-15 0.6327402801582025 1108 16-17 0.6998599519395512 1108 18-19 0.7478485770454739 1108 20-21 0.6811785216808985 1108 22-23 0.660309764977022 1108 24-25 0.7446267207698938 1108 26-27 0.7530741845071063 1108 28-29 0.8844886301691233 1108 30-31 0.9285102529601374 1108 32-33 0.9569813867748493 1108 34-35 1.1429366608922251 1108 36-37 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0.042790468136345794 2311 12-13 0.10556516463685739 2311 14-15 0.17179023380811032 2311 16-17 0.3372324891828242 2311 18-19 0.05339198292867309 2311 20-21 0.010030801974927783 2311 22-23 -0.02781286039671471 2311 24-25 -0.04274059392765395 2311 26-27 0.3565687204186432 2311 28-29 0.16540610089023033 2311 30-31 0.26309427719274936 2311 32-33 0.007772276043816362 2311 34-35 0.05503142398449512 2311 36-37 0.49099946201332045 2311 38-39 0.2436574829257694 2311 40-41 -0.07308775007607693 2311 42-43 0.5319102957760151 2311 44-45 0.47092504282048964 2311 46-47 0.3484210259080456 2311 48-49 0.22403960852158633 2311 50-51 0.21949444941193264 2311 52-53 0.4556576564528356 2311 54-55 0.3034915805582017 2311 56-57 0.4469575525889269 2311 58-59 0.420745018773637 2311 60-61 0.5879683809203371 2311 62-63 1.0957409334376962 2311 64-65 1.0507481067385527 2311 66-67 0.9318259325083496 2311 68-69 0.8818723227730807 2311 70-71 0.8112832499106588 2311 72-73 0.8251137055342852 2311 74-75 1.147107381158083 2311 76-77 1.120708619389326 2311 78-79 1.5212670407819786 2311 80-81 1.3679259775830523 2311 82-83 1.5558516606174386 2311 84-85 1.7992014230900075 2311 86-87 2.220161214003518 2311 88-89 2.1261024741229484 2311 90-91 2.436243129289881 2311 92-93 2.59406950988496 2311 94-95 2.8242062873982334 2311 96-97 2.707652226520999 2311 98-99 2.9452548708170703 2311 100-101 3.5260807572248822 2312 1 -0.5575465164416968 2312 2 -0.50396144082384 2312 3 -0.30871506833984697 2312 4 -0.423156784167233 2312 5 -0.27245425001910917 2312 6 -0.14527860606985143 2312 7 -0.2790027202247387 2312 8 -0.3340749940426875 2312 9 -0.1937898242367453 2312 10-11 -0.274156248712373 2312 12-13 -0.2591403839148825 2312 14-15 -0.17574728770176762 2312 16-17 -0.279635549821748 2312 18-19 -0.11543410682848076 2312 20-21 -0.2915269579916995 2312 22-23 -0.4375196819038294 2312 24-25 -0.5308300259441694 2312 26-27 -0.26954741605444354 2312 28-29 -0.09180143377863459 2312 30-31 -0.16840231956872742 2312 32-33 -0.33087449571068106 2312 34-35 -0.4053413218875974 2312 36-37 -0.42741330066792216 2312 38-39 -0.4614823427866952 2312 40-41 -0.41902410844215154 2312 42-43 -0.40763122557436304 2312 44-45 -0.3395687104960885 2312 46-47 -0.37588751393545294 2312 48-49 -0.14662352454366356 2312 50-51 -0.1926562930431217 2312 52-53 -0.11949195414551639 2312 54-55 0.013140428917004954 2312 56-57 -0.20175430859452348 2312 58-59 0.12240798926867313 2312 60-61 0.2605021449113494 2312 62-63 0.07735209618275007 2312 64-65 0.22574036164863287 2312 66-67 0.4915359747459327 2312 68-69 0.532385492493308 2312 70-71 0.5910765403622662 2312 72-73 0.7783318287379579 2312 74-75 0.9521657684003095 2312 76-77 1.081434878771617 2312 78-79 1.1234930256353444 2312 80-81 1.178822322207349 2312 82-83 1.5056558095317456 2312 84-85 1.7135123554761407 2312 86-87 2.146280724575181 2312 88-89 2.098513083515826 2312 90-91 2.126181107223278 2312 92-93 2.304854049151796 2312 94-95 2.3046449508871163 2312 96-97 2.3857639889353806 2312 98-99 2.834641950473401 2312 100-101 2.442013557450636 2313 1 -0.24709081479729988 2313 2 -0.351390655407247 2313 3 -0.28101740058647096 2313 4 -0.4035362967002243 2313 5 -0.3034135401945335 2313 6 -0.22197972502278418 2313 7 -0.10548739283845521 2313 8 -0.05279476619186596 2313 9 -0.15563257373220551 2313 10-11 -0.271961184285999 2313 12-13 -0.09590476490821231 2313 14-15 -0.3046612346082327 2313 16-17 -0.20709691788202633 2313 18-19 -0.10256823787852909 2313 20-21 0.030864639710692643 2313 22-23 -0.19611782613770146 2313 24-25 -0.13574231293111438 2313 26-27 -0.041568218786743216 2313 28-29 -0.13158352456070332 2313 30-31 0.08213133261245531 2313 32-33 -0.15926613520959876 2313 34-35 -0.04517013522427504 2313 36-37 -0.0470334895882587 2313 38-39 -0.2509509595602424 2313 40-41 -0.059122360152827014 2313 42-43 0.031067439324914403 2313 44-45 0.05240217523218149 2313 46-47 0.055064611119782114 2313 48-49 -0.011637884638723506 2313 50-51 -0.09137582635496244 2313 52-53 -0.033047764460903295 2313 54-55 -0.10198290071434712 2313 56-57 0.019402350787601108 2313 58-59 0.0583712202602058 2313 60-61 0.11592942491230218 2313 62-63 -0.1231914080387071 2313 64-65 -0.013526885392774801 2313 66-67 0.29115667119561195 2313 68-69 0.5781519513714883 2313 70-71 0.6285695245154095 2313 72-73 0.5016096622444515 2313 74-75 0.7721376453035482 2313 76-77 0.9710009710344849 2313 78-79 1.5318488992426254 2313 80-81 1.156782113941187 2313 82-83 1.374882663208048 2313 84-85 1.625256733842253 2313 86-87 1.7982805394762416 2313 88-89 1.8550340892598634 2313 90-91 2.033135304192431 2313 92-93 2.38319045751588 2313 94-95 2.460969960739156 2313 96-97 2.7326640295377302 2313 98-99 2.5516182100418234 2313 100-101 2.6399238800004667 2314 1 -0.2655756492389898 2314 2 -0.4205422414767135 2314 3 -0.4312392649421959 2314 4 -0.39992158761479146 2314 5 -0.47930621114014826 2314 6 -0.35552410947953916 2314 7 -0.4511207779208277 2314 8 -0.33338366254703544 2314 9 -0.48029021907363045 2314 10-11 -0.8032061155833574 2314 12-13 -0.5623890548264114 2314 14-15 -0.6088268392610132 2314 16-17 -0.6359714341829772 2314 18-19 -0.504777033670635 2314 20-21 -0.6223723786177402 2314 22-23 -0.8205877846612353 2314 24-25 -0.8342790508878792 2314 26-27 -0.5905637467428093 2314 28-29 -0.6273851905708554 2314 30-31 -0.9374961322252346 2314 32-33 -0.5924678753676247 2314 34-35 -0.8170872054562253 2314 36-37 -0.658339303245377 2314 38-39 -0.544966125118556 2314 40-41 -0.6186361701952308 2314 42-43 -0.5899166999804208 2314 44-45 -0.5496901852191982 2314 46-47 -0.47264236573217744 2314 48-49 0.061144340724489155 2314 50-51 -0.10620829849158042 2314 52-53 -0.14094451353984283 2314 54-55 -0.3454558599098476 2314 56-57 -0.2945806067859138 2314 58-59 0.2147102846066602 2314 60-61 0.13979287695948273 2314 62-63 0.24678188713936322 2314 64-65 0.20248094647691062 2314 66-67 0.38627304860831657 2314 68-69 0.3908460102727105 2314 70-71 0.45182241512411636 2314 72-73 0.6634504412606042 2314 74-75 0.6712936339033106 2314 76-77 0.8231425703115391 2314 78-79 0.865370981407743 2314 80-81 1.0754888127386018 2314 82-83 0.949469346712462 2314 84-85 1.298393478750377 2314 86-87 1.6498819598715428 2314 88-89 1.6636934608565959 2314 90-91 2.1953670974125323 2314 92-93 2.341275183052872 2314 94-95 2.581309650708338 2314 96-97 3.005002526189486 2314 98-99 3.393842530546028 2314 100-101 3.510624171957737 2315 1 -0.7118995928191758 2315 2 -0.603224331262961 2315 3 -0.43519085864519624 2315 4 -0.4652375699385942 2315 5 -0.3563509648986667 2315 6 -0.34034778303899316 2315 7 -0.386745512981129 2315 8 -0.50306701243003 2315 9 -0.6462368304586334 2315 10-11 -0.587883455327507 2315 12-13 -0.6842061491710751 2315 14-15 -0.8374400360161189 2315 16-17 -0.7169341016393815 2315 18-19 -0.5350411313658796 2315 20-21 -0.42309562769299447 2315 22-23 -0.7197873038501257 2315 24-25 -0.668524067118156 2315 26-27 -0.6225452343538009 2315 28-29 -0.5696297887045105 2315 30-31 -0.6521495475519572 2315 32-33 -0.5018933409356308 2315 34-35 -0.7834623658717632 2315 36-37 -0.6806319824897784 2315 38-39 -0.4252845173047888 2315 40-41 -0.6272710266777608 2315 42-43 -0.6353585758405629 2315 44-45 -0.7643247281292602 2315 46-47 -0.6981125792518057 2315 48-49 -0.7780597659133761 2315 50-51 -0.7703939682300955 2315 52-53 -0.6648834482064458 2315 54-55 -0.5633088757220293 2315 56-57 -0.41862029112296284 2315 58-59 -0.11950714886081215 2315 60-61 -0.3358428331369723 2315 62-63 -0.16117152992942607 2315 64-65 -0.48537508892193415 2315 66-67 -0.188458817902724 2315 68-69 0.06997718227881577 2315 70-71 0.14101548865685842 2315 72-73 0.5943933652657343 2315 74-75 0.4539254940267661 2315 76-77 0.8072723196277778 2315 78-79 0.9746727600604217 2315 80-81 1.2398762721629062 2315 82-83 1.1732831427393862 2315 84-85 1.2764405030057908 2315 86-87 1.2248286815875993 2315 88-89 1.4575442468201594 2315 90-91 1.5987504862017374 2315 92-93 1.9250091923090693 2315 94-95 1.58343352344437 2315 96-97 1.708990122656573 2315 98-99 2.286709628084484 2315 100-101 2.6859532778697393 2316 1 -1.0104311586382444 2316 2 -0.825079853519302 2316 3 -0.6789503198107489 2316 4 -0.7105598471147374 2316 5 -0.6745954628670034 2316 6 -0.5664106511434497 2316 7 -0.5839667648274016 2316 8 -0.5841005547517 2316 9 -0.7855450239046107 2316 10-11 -0.8542302043637733 2316 12-13 -0.7738091326174867 2316 14-15 -1.0517174327936516 2316 16-17 -0.9234602374389027 2316 18-19 -0.8864357148294459 2316 20-21 -0.7397022331953238 2316 22-23 -1.1682143755006464 2316 24-25 -0.964078621654302 2316 26-27 -0.8935160801743365 2316 28-29 -0.8962062410508835 2316 30-31 -0.8589055092353846 2316 32-33 -0.8275198462951678 2316 34-35 -0.7619751778987123 2316 36-37 -0.8521587005148703 2316 38-39 -0.8215211950643919 2316 40-41 -0.8510279750803136 2316 42-43 -0.7366060707384321 2316 44-45 -0.7652838681514496 2316 46-47 -0.5465408766403641 2316 48-49 -0.0046880056922518065 2316 50-51 -0.14023437713630926 2316 52-53 -0.390773180811685 2316 54-55 -0.32509281487638475 2316 56-57 -0.2958610934910837 2316 58-59 -0.39029413397421564 2316 60-61 -0.18237276872444141 2316 62-63 0.16547999887235676 2316 64-65 -0.09650969334562376 2316 66-67 0.12282042361057322 2316 68-69 0.14956895022209338 2316 70-71 0.056729143991574915 2316 72-73 0.3095732116807568 2316 74-75 0.6102285455016343 2316 76-77 0.6211593188371793 2316 78-79 0.5432068386424902 2316 80-81 1.039088122598347 2316 82-83 1.4482805380460348 2316 84-85 1.398677229794906 2316 86-87 1.706767431457365 2316 88-89 1.6994298609455676 2316 90-91 2.1156733321203376 2316 92-93 2.2882597270372926 2316 94-95 2.2764305755443672 2316 96-97 1.8807757778441996 2316 98-99 2.103478643878116 2316 100-101 1.963654957743735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4562.0 3 902.0 4 805.0 5 745.0 6 784.0 7 952.0 8 1160.0 9 1424.0 10 1863.0 11 2533.0 12 3471.0 13 4448.0 14 5546.0 15 6814.0 16 8076.0 17 9731.0 18 9097.0 19 7972.0 20 8335.0 21 8736.0 22 9937.0 23 10645.0 24 12379.0 25 14218.0 26 16006.0 27 18415.0 28 21357.0 29 24716.0 30 28272.0 31 32891.0 32 39006.0 33 45581.0 34 54981.0 35 66805.0 36 80749.0 37 95044.0 38 69000.0 39 6552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.343213666762715 20.920504377728825 15.183228197735954 22.553053757772503 2 15.187908073904788 22.215155221303057 40.15528940049739 22.441647304294772 3 18.80410680526726 25.301554353333444 31.566094067524205 24.328244773875095 4 12.317073835156693 18.716493047988493 38.81712286972061 30.149310247134203 5 12.691363541005401 38.083626446380066 35.83562420137479 13.389385811239741 6 29.42364827955422 38.03820548902508 18.545859255697245 13.992286975723452 7 25.718896613712662 33.80921576771962 22.78243461326324 17.68945300530448 8 23.692145518729163 35.57832690505764 21.68548843999913 19.04403913621407 9 24.533480066411244 17.35667987355168 22.915829602581265 35.1940104574558 10-11 23.002490226694388 27.719416754452208 29.463460672871918 19.814632345981487 12-13 24.310712430090657 25.782341136486053 28.97517141536075 20.931775018062538 14-15 21.33509555705812 27.959708538545918 28.04999867220392 22.65519723219204 16-17 19.888295999940084 30.463272697066728 29.163214073914194 20.48521722907899 18-19 19.52863970345414 29.583636050422058 31.24285479861341 19.644869447510388 20-21 21.08593562727451 28.371927368214607 30.861562683867593 19.68057432064329 22-23 20.237908788693034 28.861268220403037 30.54681297010785 20.35401002079608 24-25 20.386790942866774 28.680286193967586 30.853575892142604 20.079346971023032 26-27 20.34634864919532 28.648269108051434 31.163410283808936 19.84197195894431 28-29 19.693514194870385 29.460323210889282 30.88413452796923 19.962028066271102 30-31 20.3769380795171 28.998872291046972 30.748251233942405 19.875938395493524 32-33 20.07288906684426 28.91402508489765 30.78943408149493 20.223651766763158 34-35 20.576859434348993 28.876610615455643 30.562913591612883 19.98361635858248 36-37 20.256579619352422 28.64247046406319 30.821694869088557 20.279255047495827 38-39 19.950991639710253 29.024483073460118 30.840679253967206 20.183846032862427 40-41 20.391367074973328 28.88178987696332 30.55933995529529 20.16750309276806 42-43 20.130276437422168 28.77642686878329 30.44903633973186 20.64426035406268 44-45 20.521103450717316 28.606839346632785 30.339584568682458 20.532472633967444 46-47 20.529794561118987 28.728558414660547 30.067717077966556 20.67392994625391 48-49 20.242946953903804 28.58949971337107 30.34392748356139 20.823625849163736 50-51 20.13029848155296 28.811213566291226 30.27826282306569 20.780225129090123 52-53 20.390099648866748 29.038134514397278 29.795610221667328 20.77615561506864 54-55 20.35984358533776 28.61538712901214 29.834653581280346 21.190115704369752 56-57 20.641124176368034 28.581075883402505 29.596834748061713 21.180965192167744 58-59 20.656879397378336 28.440651773587987 29.628126882767212 21.27434194626646 60-61 20.473094913756544 28.37146671404832 29.944115518648694 21.21132285354644 62-63 20.407731194492968 28.201623191405062 29.98499616735128 21.40564944675069 64-65 20.269055914777855 28.541970462532184 29.580288566927678 21.608685055762283 66-67 20.373617755897257 28.277970956876437 29.44421601810226 21.90419526912405 68-69 20.36982448926519 28.12794222666693 29.650224349570053 21.85200893449782 70-71 20.427475012101624 27.927486926500418 29.713897839817378 21.931140221580584 72-73 20.383671657561777 27.484599757081934 30.147235036846205 21.984493548510084 74-75 20.47717277344335 27.952902250725053 29.48388546219517 22.086039513636425 76-77 20.38319069602152 27.687750574078017 29.620723859111415 22.308334870789047 78-79 20.327023593787654 27.634439931760596 29.741665112264165 22.29687136218758 80-81 20.590652037314516 27.47707668019816 29.660587139250023 22.271684143237305 82-83 20.530528574647832 27.422716067201463 29.777504243152915 22.269251114997786 84-85 20.659201964930244 27.370421754740438 29.635292499853637 22.335083780475685 86-87 20.632196622884198 27.26192218542483 29.641423957032142 22.464457234658834 88-89 20.93739744513272 27.172052513538947 29.432719241029297 22.457830800299032 90-91 21.023767614624862 26.86717577003871 29.508271511206292 22.60078510413014 92-93 21.192352809418672 26.81500271985741 29.584627733558822 22.4080167371651 94-95 21.099228969608166 26.774581870572568 29.473392506488803 22.652796653330466 96-97 21.015546595531156 26.58580857411697 29.480046243530193 22.918598586821684 98-99 21.34492811246019 26.439146472479308 29.184679968028803 23.031245447031704 100-101 21.663316137737404 25.923144545225323 29.580014038148327 22.833525278888942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 680.0 1 396.0 2 133.5 3 154.5 4 133.0 5 133.0 6 204.5 7 356.0 8 496.5 9 546.0 10 479.5 11 404.0 12 425.0 13 452.0 14 517.5 15 611.5 16 728.0 17 845.5 18 959.0 19 1138.0 20 1410.5 21 1701.0 22 2066.0 23 2556.0 24 3199.0 25 4010.5 26 5004.5 27 6429.0 28 7971.5 29 9813.5 30 12012.0 31 13987.5 32 16085.5 33 18346.5 34 20591.0 35 22617.0 36 24242.5 37 25727.0 38 26722.5 39 27353.5 40 28072.0 41 28788.5 42 29263.5 43 29665.5 44 29956.5 45 29724.5 46 29704.5 47 30885.0 48 33048.5 49 32255.0 50 28196.5 51 24692.5 52 21771.0 53 18754.5 54 16062.0 55 13729.5 56 11370.0 57 9136.0 58 7283.5 59 5648.0 60 4211.0 61 3084.5 62 2177.5 63 1527.0 64 1078.0 65 768.5 66 546.0 67 412.0 68 332.5 69 255.5 70 189.5 71 140.5 72 110.0 73 85.5 74 74.5 75 62.5 76 37.0 77 25.5 78 24.0 79 16.5 80 14.0 81 12.5 82 5.0 83 4.0 84 4.0 85 1.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3105471674994214 2 0.03648690964044057 3 0.04274958816081469 4 0.04547249186532518 5 0.05935930075832869 6 0.05813399409129896 7 0.030632666675743013 8 0.03458087704728322 9 0.040435120011980775 10-11 0.03260677186151312 12-13 0.03478509482512151 14-15 0.02865856148997291 16-17 0.021579011858245633 18-19 0.02566336741501137 20-21 0.038937522974500004 22-23 0.032266408898449304 24-25 0.05343698520101837 26-27 0.045812854828388996 28-29 0.03859716001143619 30-31 0.03784836149269581 32-33 0.03315135260241522 34-35 0.032810989639351404 36-37 0.03199411852799826 38-39 0.019809124450313815 40-41 0.01844767259805857 42-43 0.020217560005990388 44-45 0.009325945187948428 46-47 0.01783501926454371 48-49 0.01511211556003322 50-51 0.005786170372084791 52-53 0.0042205007419912595 54-55 0.007079549631727274 56-57 0.004697008890280596 58-59 0.007147622224340036 60-61 0.006603041483437939 62-63 0.0042205007419912595 64-65 0.007215694816952799 66-67 0.0021783229636083918 68-69 0.00728376740956556 70-71 0.014227171856067311 72-73 0.014431389633905597 74-75 0.01089161481804196 76-77 0.009734380743625001 78-79 0.005037371853344406 80-81 0.006875331853888987 82-83 0.012457284448135491 84-85 0.0042205007419912595 86-87 0.0010210888891914339 88-89 0.0200133422281521 90-91 0.028930851860423958 92-93 0.013138010374263114 94-95 0.022872391117888115 96-97 0.018651890375896856 98-99 0.013478373337326925 100-101 0.10823542225429199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 734510.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.50406805550291 #Duplication Level Percentage of deduplicated Percentage of total 1 78.11911579225996 53.514772246686896 2 13.778261915746604 18.877339839256983 3 4.386319013105073 9.014420885606889 4 1.7384017003931602 4.7635035356614015 5 0.8121712957053656 2.7818518856863172 6 0.4364469996266487 1.79390369790264 7 0.24302784928357987 1.1653877428693404 8 0.13845183178913065 0.7587610965833319 9 0.08582250360987174 0.5291271565185869 >10 0.24067654806801497 2.6586171173168602 >50 0.010552926156960704 0.511085511406222 >100 0.00796416612360885 1.152396659534915 >500 0.0015928332182986173 0.6771984143817653 >1k 0.001194624913723963 1.8016342105878627 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3574 0.4865828919960246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.42627057494111725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.28290969489863993 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2042 0.278008468230521 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1280 0.17426583708867135 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1104 0.15030428448897906 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 905 0.12321139262909968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 778 0.10592095410545807 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.361451852255245E-4 0.0 16-17 0.0 0.0 0.0 1.361451852255245E-4 0.0 18-19 0.0 0.0 0.0 1.361451852255245E-4 0.0 20-21 0.0 0.0 0.0 8.16871111353147E-4 0.0 22-23 0.0 0.0 0.0 0.0024506133340594406 0.0 24-25 0.0 0.0 0.0 0.004424718519829546 0.0 26-27 0.0 0.0 0.0 0.009598235558399478 0.0 28-29 0.0 0.0 0.0 0.026752528896815564 0.0 30-31 0.0 0.0 0.0 0.07065935113204722 0.0 32-33 0.0 0.0 0.0 0.15677118078719146 0.0 34-35 0.0 0.0 0.0 0.3025826741637282 0.0 36-37 0.0 0.0 0.0 0.5173517038569931 0.0 38-39 0.0 0.0 0.0 0.7644552150413201 0.0 40-41 0.0 0.0 0.0 0.9964466106656138 0.0 42-43 0.0 0.0 0.0 1.238921185552273 0.0 44-45 0.0 0.0 0.0 1.5501490789778218 0.0 46-47 0.0 0.0 0.0 1.9059645205647302 0.0 48-49 0.0 0.0 0.0 2.243059999183129 0.0 50-51 0.0 0.0 0.0 2.5627288940926602 0.0 52-53 0.0 0.0 0.0 2.8694639964057673 0.0 54-55 0.0 0.0 0.0 3.240595771330547 0.0 56-57 0.0 0.0 0.0 3.6970225047991176 0.0 58-59 0.0 0.0 0.0 4.141264244190005 0.0 60-61 0.0 0.0 0.0 4.582646934691155 0.0 62-63 0.0 0.0 0.0 5.017835019264544 0.0 64-65 0.0 0.0 0.0 5.505507072742372 0.0 66-67 0.0 0.0 0.0 6.06656138105676 0.0 68-69 0.0 0.0 0.0 6.682073763461355 0.0 70-71 0.0 0.0 0.0 7.318484431798069 0.0 72-73 0.0 0.0 0.0 7.919905787531825 0.0 74-75 0.0 0.0 0.0 8.579801500319942 0.0 76-77 0.0 0.0 0.0 9.285850430899512 0.0 78-79 0.0 0.0 0.0 10.057793631128234 0.0 80-81 0.0 0.0 0.0 10.848456794325468 0.0 82-83 0.0 0.0 0.0 11.63959646567099 0.0 84-85 0.0 0.0 0.0 12.43311867775796 0.0 86-87 0.0 0.0 0.0 13.272794107636383 0.0 88-89 0.0 0.0 0.0 14.162979401233475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 130 0.0 25.59742 94-95 CGCCGCA 60 1.7129035E-4 23.746376 92-93 AGTACTT 2970 0.0 20.875134 12-13 CGCCGTA 160 1.8189894E-11 20.778076 92-93 GTACATG 2230 0.0 20.730276 1 GACGCTC 105 8.5450483E-7 20.373459 94-95 GCCGTAT 175 3.6379788E-12 20.373459 94-95 TACATGG 2235 0.0 19.776897 2 ACATGGG 2210 0.0 19.140375 3 GAGTACT 3200 0.0 18.780872 12-13 GTAAGCG 65 0.007529236 18.28387 94-95 TCGCCGT 195 1.4551915E-11 18.266441 92-93 GTACTTT 3190 0.0 18.020626 14-15 TAGCCCT 745 0.0 17.863003 4 GTAGCCC 480 0.0 17.823132 3 GTATTAG 190 1.8176816E-4 17.558306 1 GTACTAG 300 7.382732E-8 17.474697 1 AGAGTAC 4630 0.0 17.187353 10-11 GATCAAT 195 2.2168514E-4 17.108093 1 TCTCGGG 270 0.0 16.726357 94-95 >>END_MODULE