FastQCFastQC Report
Thu 26 May 2016
SRR938561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734510
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.4374344801296102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29780.405440361601612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.28454343712134617No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19640.2673891437829301No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17750.241657703775306No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13080.17807790227498604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11310.15398020449006822No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10180.13859579855958395No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT9730.13246926522443533RNA PCR Primer, Index 23 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.10483179262365386No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT1004.954725E-528.4966099
CAGCGTC700.0083250427.1858942
GGGAGCG1056.90809E-527.1396278
AGGACGT1601.8402225E-623.7763065
GAGCGTA701.7815582E-523.74717510-11
CTCCGAC1550.022.98113694-95
GTACATG21000.022.4283621
GGACGTG1302.9424747E-421.920476
TAGGACG1553.870756E-521.4753744
GTATAGC4250.021.2689631
TACATGG20850.021.2206442
AGTACTT28000.021.03321312-13
AGCGTAC704.8565166E-420.35472110-11
GTACGCG704.8565166E-420.35472114-15
ACATGGG21350.019.600143
GAGTACT29350.019.49938412-13
CATGGGG9000.019.0210484
ACGCGTT757.728634E-418.9977416-17
AGAGTAC42100.018.83980210-11
ATAGCCC4300.018.7998733
Produced by FastQC (version 0.11.5)