##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 734510 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78055438319424 31.0 31.0 34.0 30.0 34.0 2 32.01677580972349 31.0 31.0 34.0 30.0 34.0 3 32.079500619460596 33.0 31.0 34.0 30.0 34.0 4 35.746418700902645 37.0 35.0 37.0 33.0 37.0 5 35.615983444745474 37.0 35.0 37.0 33.0 37.0 6 35.65534165634232 37.0 35.0 37.0 33.0 37.0 7 35.59203686811616 37.0 35.0 37.0 33.0 37.0 8 35.594550108235424 37.0 35.0 37.0 33.0 37.0 9 37.15721637554288 39.0 37.0 39.0 33.0 39.0 10-11 37.10240568542294 39.0 37.0 39.0 33.0 39.0 12-13 37.01869545683517 39.0 37.0 39.0 33.0 39.0 14-15 38.07302555445126 40.0 37.0 41.0 33.0 41.0 16-17 38.00854106819513 40.0 37.0 41.0 33.0 41.0 18-19 38.04738601244367 40.0 37.0 41.0 33.0 41.0 20-21 38.06462335434507 40.0 37.0 41.0 33.0 41.0 22-23 38.0696083103021 40.0 37.0 41.0 33.0 41.0 24-25 38.007277640876225 40.0 37.0 41.0 33.0 41.0 26-27 37.84013151624893 40.0 37.0 41.0 32.5 41.0 28-29 37.72393704646635 40.0 37.0 41.0 32.0 41.0 30-31 37.507022368653935 39.0 36.5 41.0 32.0 41.0 32-33 37.2834141128099 39.0 36.0 41.0 31.5 41.0 34-35 37.09944725054798 39.0 36.0 41.0 31.0 41.0 36-37 37.095582088739434 39.0 36.0 41.0 31.0 41.0 38-39 36.96492695810812 39.0 36.0 41.0 31.0 41.0 40-41 36.83153190562416 39.0 35.5 41.0 31.0 41.0 42-43 36.619702931205836 39.0 35.0 41.0 30.0 41.0 44-45 36.3254625532668 39.0 35.0 41.0 30.0 41.0 46-47 35.977738900763775 39.0 35.0 40.0 29.5 41.0 48-49 35.610617282269814 39.0 35.0 40.0 27.5 41.0 50-51 35.27226109923623 38.5 35.0 40.0 27.0 41.0 52-53 34.84102258648623 38.0 34.0 40.0 25.5 41.0 54-55 34.58885379368559 38.0 34.0 40.0 24.0 41.0 56-57 34.25542130127568 38.0 33.0 40.0 23.5 41.0 58-59 33.85244857115629 38.0 33.0 40.0 22.5 41.0 60-61 33.492054566990234 37.5 32.5 40.0 21.5 41.0 62-63 33.07292684919197 37.0 32.0 40.0 19.5 41.0 64-65 32.64018733577487 36.5 31.0 39.0 18.0 40.5 66-67 32.175621162407595 36.0 31.0 39.0 13.5 40.0 68-69 31.650000680725928 35.5 30.5 39.0 9.5 40.0 70-71 31.14031531224898 35.0 30.0 38.0 8.0 40.0 72-73 30.592987161509033 34.5 29.0 37.5 7.0 39.5 74-75 30.002300172904384 34.0 29.0 37.0 2.0 39.0 76-77 28.62991041646812 32.5 27.0 35.5 2.0 38.0 78-79 29.281211964438878 34.0 28.5 36.0 2.0 38.0 80-81 29.00472151502362 34.0 28.0 36.0 2.0 37.5 82-83 28.54884004302188 34.0 27.5 35.0 2.0 37.0 84-85 27.9989891219997 33.0 26.5 35.0 2.0 36.5 86-87 27.389356850144992 33.0 26.0 35.0 2.0 36.0 88-89 26.70496385345332 32.0 25.0 35.0 2.0 35.5 90-91 26.203854270193737 32.0 24.5 34.5 2.0 35.0 92-93 25.4990646825775 32.0 21.5 34.0 2.0 35.0 94-95 24.79976991463697 31.0 19.0 34.0 2.0 35.0 96-97 23.888597160011436 31.0 6.0 34.0 2.0 35.0 98-99 22.78546582075125 31.0 2.0 34.0 2.0 35.0 100-101 19.59998366257777 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2453869043998047 1101 2 0.2736709428547357 1101 3 0.2336734269062717 1101 4 0.1230969341130006 1101 5 0.03703715777467664 1101 6 0.10366417265628058 1101 7 0.26522421028048626 1101 8 0.14808231228293067 1101 9 0.19348055622558036 1101 10-11 0.23785873728646578 1101 12-13 0.342071341964548 1101 14-15 0.4765914536815359 1101 16-17 0.4607581645470802 1101 18-19 0.5754451163928422 1101 20-21 0.4440287751348677 1101 22-23 0.5794246386815018 1101 24-25 0.5122867883332205 1101 26-27 0.32453829646537713 1101 28-29 0.3555186550713927 1101 30-31 0.5338692323005318 1101 32-33 0.2584309302519472 1101 34-35 0.3504238132271027 1101 36-37 0.5042728840352879 1101 38-39 0.47941831034870575 1101 40-41 0.5279655410291539 1101 42-43 0.4698958824631063 1101 44-45 0.4839909907655695 1101 46-47 0.6258889882762801 1101 48-49 0.5278312176259661 1101 50-51 0.7291435725365858 1101 52-53 0.8821557968154679 1101 54-55 0.8152701265523064 1101 56-57 0.9543272160708938 1101 58-59 1.1078165168942604 1101 60-61 1.088685646979421 1101 62-63 0.9346056199369457 1101 64-65 0.8740827599984229 1101 66-67 0.8699463312791593 1101 68-69 0.7924050629508343 1101 70-71 0.8314996666422871 1101 72-73 0.677530310930976 1101 74-75 0.8238823939131592 1101 76-77 0.8865764912890839 1101 78-79 0.9858541856179883 1101 80-81 1.1894043019821545 1101 82-83 1.1853362951503144 1101 84-85 1.3654207939072762 1101 86-87 1.6716052783841882 1101 88-89 1.8052057758484814 1101 90-91 1.9134217705383918 1101 92-93 2.196327018907027 1101 94-95 2.101319086515641 1101 96-97 2.4157962022286164 1101 98-99 2.823279909541501 1101 100-101 3.730517942784296 1102 1 0.2058498549825991 1102 2 0.2883269787348155 1102 3 0.37578396175306494 1102 4 0.18977626764806388 1102 5 0.24145930227549428 1102 6 0.141953300964623 1102 7 0.3410471462260034 1102 8 0.3182030463044967 1102 9 0.45319827271760715 1102 10-11 0.26044212163912306 1102 12-13 0.33918141939664537 1102 14-15 0.5039894208837339 1102 16-17 0.46670103697060483 1102 18-19 0.46967441955086997 1102 20-21 0.44264480247652926 1102 22-23 0.3524273198353072 1102 24-25 0.19805227768484457 1102 26-27 0.21648873616991438 1102 28-29 0.21931697976076237 1102 30-31 0.35990973343937327 1102 32-33 0.13292454873056414 1102 34-35 0.22935435421967298 1102 36-37 0.37580386273852895 1102 38-39 0.3870099868087422 1102 40-41 0.48154615978221926 1102 42-43 0.43043976026066844 1102 44-45 0.2636648892758018 1102 46-47 0.734105094434021 1102 48-49 0.9457006503613741 1102 50-51 1.0408447095053077 1102 52-53 0.8316865362556456 1102 54-55 0.8802570257263795 1102 56-57 0.9588603129560198 1102 58-59 1.1409016734356427 1102 60-61 1.0227604612573273 1102 62-63 0.9599721967626778 1102 64-65 1.0729232723967073 1102 66-67 1.11611049858503 1102 68-69 0.8461770921596923 1102 70-71 1.2116335601326966 1102 72-73 1.282419018417798 1102 74-75 1.2666363587602518 1102 76-77 1.235539332796062 1102 78-79 1.5255378423083243 1102 80-81 1.73727920023671 1102 82-83 1.7106641676559384 1102 84-85 1.8425946279103123 1102 86-87 1.677867168234041 1102 88-89 1.884562533505484 1102 90-91 2.1644568178866734 1102 92-93 2.6034378235111966 1102 94-95 2.7170520407812084 1102 96-97 2.657782648679426 1102 98-99 3.013842388869879 1102 100-101 3.4575727316815517 1103 1 0.1512548224784993 1103 2 0.26373978409881715 1103 3 0.3080292145737076 1103 4 0.188393114453973 1103 5 0.10890053473085715 1103 6 0.13226464216216982 1103 7 0.23901654560820162 1103 8 0.1794332622739887 1103 9 0.2674605580818721 1103 10-11 0.22685455157583334 1103 12-13 0.2810593462476092 1103 14-15 0.5282110678898633 1103 16-17 0.27847035336360904 1103 18-19 0.2903255553831059 1103 20-21 0.419984142646598 1103 22-23 0.32076628457807743 1103 24-25 0.46797462351266006 1103 26-27 0.3527754308948303 1103 28-29 0.3852657240552517 1103 30-31 0.3809764738742203 1103 32-33 0.5215115589461377 1103 34-35 0.7639727027347547 1103 36-37 0.758443314219484 1103 38-39 0.857894612073018 1103 40-41 1.195931611645193 1103 42-43 1.0574494489194706 1103 44-45 0.955508237296911 1103 46-47 1.088689588628064 1103 48-49 1.2016366828193696 1103 50-51 1.3379460155971046 1103 52-53 1.570053229926728 1103 54-55 1.5648915110287263 1103 56-57 1.5316372235187004 1103 58-59 1.829735016648641 1103 60-61 1.7395466718786352 1103 62-63 1.552363536906391 1103 64-65 1.7416646688545185 1103 66-67 1.721845973829879 1103 68-69 1.464212751166329 1103 70-71 1.7560459505507353 1103 72-73 1.7961484604801434 1103 74-75 1.8163146109815322 1103 76-77 1.8197601894289441 1103 78-79 1.9325537222242808 1103 80-81 2.102840002992476 1103 82-83 2.2216437694395488 1103 84-85 2.282605297949239 1103 86-87 2.5396308861498262 1103 88-89 2.6109550723819694 1103 90-91 2.7129264576740653 1103 92-93 2.6196338887696804 1103 94-95 2.6007486164890885 1103 96-97 3.161882709933355 1103 98-99 3.54824997118396 1103 100-101 4.118410395601668 1104 1 0.21443668651643222 1104 2 0.2367462550515853 1104 3 0.1676220983786223 1104 4 0.1712820458923403 1104 5 0.11044878687360438 1104 6 0.17443392459259854 1104 7 0.16093275469635415 1104 8 0.11177134948258072 1104 9 0.35474944916686013 1104 10-11 0.46828717893089333 1104 12-13 0.33594509059246747 1104 14-15 0.2716798938741434 1104 16-17 0.3005154462072639 1104 18-19 0.5761479305089523 1104 20-21 0.6449676204079751 1104 22-23 0.5664154284069909 1104 24-25 0.6639417560961576 1104 26-27 0.6247675084556761 1104 28-29 0.7913524961760103 1104 30-31 0.5602165126628691 1104 32-33 0.5017486968208686 1104 34-35 0.5991353206808938 1104 36-37 0.6010764546389566 1104 38-39 0.45360006533824304 1104 40-41 0.5033780456210977 1104 42-43 0.3369254314522223 1104 44-45 0.37911990601212153 1104 46-47 0.148790389725022 1104 48-49 0.256193011814716 1104 50-51 0.13494998299322702 1104 52-53 0.3618225815343834 1104 54-55 0.43206815383337727 1104 56-57 0.3066856578693091 1104 58-59 0.3820647037658276 1104 60-61 0.11687960167815703 1104 62-63 0.25315969262926785 1104 64-65 0.2048607389293977 1104 66-67 0.2524949838338131 1104 68-69 0.2452802216064498 1104 70-71 0.16557614249693842 1104 72-73 0.16130949311490994 1104 74-75 0.367512962257706 1104 76-77 0.2832661158169678 1104 78-79 0.397725254973313 1104 80-81 0.6600851847782785 1104 82-83 0.8893470650342366 1104 84-85 0.9191294307118589 1104 86-87 1.1341020612773214 1104 88-89 1.2035979923196258 1104 90-91 1.5282675363858047 1104 92-93 1.4356852693288218 1104 94-95 1.5077392219750898 1104 96-97 2.0904757563870966 1104 98-99 2.3634459012573146 1104 100-101 3.2418608566802156 1105 1 0.38717936839331557 1105 2 0.38750766271409276 1105 3 0.47208330925126063 1105 4 0.3462885799308424 1105 5 0.24270733493337815 1105 6 0.3378944282516585 1105 7 0.27141758035283203 1105 8 0.33187812335872735 1105 9 0.5612750318493553 1105 10-11 0.4674761894468489 1105 12-13 0.46393345231683725 1105 14-15 0.5050786316516493 1105 16-17 0.4877915592999429 1105 18-19 0.46805955956006073 1105 20-21 0.6719272554242721 1105 22-23 0.24413743197381166 1105 24-25 0.31022254294813933 1105 26-27 0.45193450310544847 1105 28-29 0.4442374663500459 1105 30-31 0.6330085841836919 1105 32-33 0.558763948035434 1105 34-35 0.6695799427828533 1105 36-37 0.6361498280477278 1105 38-39 0.9496421676074789 1105 40-41 0.8823695744323601 1105 42-43 1.1779580401478782 1105 44-45 1.0812676829016112 1105 46-47 1.1051007334923142 1105 48-49 1.1052029128048133 1105 50-51 1.1807862630498036 1105 52-53 1.1402139396919324 1105 54-55 1.177873877651102 1105 56-57 1.0862569865083884 1105 58-59 1.2756492388497662 1105 60-61 1.3491896533355003 1105 62-63 1.5942576417098877 1105 64-65 1.581134876436316 1105 66-67 1.6529786948601952 1105 68-69 1.5714152225288984 1105 70-71 1.626280627153477 1105 72-73 1.6410040075974202 1105 74-75 1.7192469807682542 1105 76-77 1.7011620785498849 1105 78-79 2.092128274467811 1105 80-81 2.1668104399901367 1105 82-83 2.0996835295813128 1105 84-85 2.119738825890508 1105 86-87 1.986931492124132 1105 88-89 2.1650942486999227 1105 90-91 2.0401248944818633 1105 92-93 2.3822675828396775 1105 94-95 2.677268694332266 1105 96-97 3.0319403803339604 1105 98-99 3.5438228384075536 1105 100-101 4.270563542554392 1106 1 0.2953894539243187 1106 2 0.35432727544871057 1106 3 0.3345283674598605 1106 4 0.2663037260463099 1106 5 0.17647277803150274 1106 6 0.23278484165151525 1106 7 0.23531191392375206 1106 8 0.2851256287044208 1106 9 0.2990244972805698 1106 10-11 0.2966072181947723 1106 12-13 0.2987294252320183 1106 14-15 0.3322384380189547 1106 16-17 0.28522340178033545 1106 18-19 0.27707001334955805 1106 20-21 0.41932494489920913 1106 22-23 0.362113792154382 1106 24-25 0.34568598438315945 1106 26-27 0.1880505639275043 1106 28-29 0.2698047032234143 1106 30-31 0.29730310188385545 1106 32-33 0.45158290740820917 1106 34-35 0.37604228538204865 1106 36-37 0.3479838444411669 1106 38-39 0.5006452265288388 1106 40-41 0.39872589422328275 1106 42-43 0.6900675079549572 1106 44-45 0.13769438758205865 1106 46-47 0.18907257953650713 1106 48-49 0.44468378712174683 1106 50-51 0.24414008818641975 1106 52-53 0.505001448601412 1106 54-55 0.38024157187775387 1106 56-57 0.23096549803130983 1106 58-59 0.4847807130716717 1106 60-61 0.46597065832481377 1106 62-63 0.4342508111087966 1106 64-65 -0.07328868022797508 1106 66-67 0.18957809495523037 1106 68-69 0.044619962372095756 1106 70-71 0.3121732085267297 1106 72-73 0.6347822209497558 1106 74-75 0.7668867111080004 1106 76-77 0.8492302063712209 1106 78-79 1.0110668584545053 1106 80-81 0.8892987091752147 1106 82-83 1.3623869627008176 1106 84-85 1.4553180905614482 1106 86-87 1.6245421181635713 1106 88-89 1.8196254615770933 1106 90-91 2.0846871420466044 1106 92-93 1.8361758745954404 1106 94-95 2.393331001293536 1106 96-97 2.6368272299419466 1106 98-99 2.905863257747061 1106 100-101 3.570089112761096 1107 1 0.14242602391171033 1107 2 0.2479800707408515 1107 3 0.1587402211799116 1107 4 0.21556812285168547 1107 5 0.2495286838742885 1107 6 0.015256468385437927 1107 7 0.15695966507970383 1107 8 0.18245643996965555 1107 9 0.16850747332092197 1107 10-11 0.19162613370549053 1107 12-13 0.35652681909498796 1107 14-15 0.46432124592143964 1107 16-17 0.4199438725663853 1107 18-19 0.29094138787667134 1107 20-21 0.2205859789471205 1107 22-23 0.21536095482776574 1107 24-25 0.35759224751893015 1107 26-27 0.3228235777988715 1107 28-29 0.32889465698550424 1107 30-31 0.24467594761735967 1107 32-33 0.5555866905103528 1107 34-35 0.42286868302812053 1107 36-37 0.6025761100990081 1107 38-39 0.6430054619218595 1107 40-41 0.7096758732060451 1107 42-43 0.7074382519650442 1107 44-45 0.9263345725337189 1107 46-47 0.7799195740720251 1107 48-49 0.7019977845996834 1107 50-51 0.8107542117677511 1107 52-53 1.0340998092571496 1107 54-55 0.9413807059677097 1107 56-57 0.9430609162742698 1107 58-59 0.9577945844461979 1107 60-61 1.118009330035612 1107 62-63 0.9049948211970644 1107 64-65 0.842568822924612 1107 66-67 1.2373397595201752 1107 68-69 1.2243068055835238 1107 70-71 1.3387074666183594 1107 72-73 1.2475396820678704 1107 74-75 1.2075865850045986 1107 76-77 1.0309767385275883 1107 78-79 1.4455981741333623 1107 80-81 1.791841794505185 1107 82-83 1.9050834738399516 1107 84-85 2.014502621877128 1107 86-87 1.98367410639392 1107 88-89 2.0858822921781837 1107 90-91 2.5147207696212135 1107 92-93 2.604229678714816 1107 94-95 2.453892161444859 1107 96-97 2.5318776713038638 1107 98-99 3.107092765943783 1107 100-101 4.027897015241127 1108 1 0.20305832354924647 1108 2 0.26580125479240024 1108 3 0.2964701178103297 1108 4 0.13142259996271122 1108 5 0.03902391813392114 1108 6 0.20645662948088273 1108 7 0.26226239027771214 1108 8 0.16114485452194316 1108 9 0.21542652285777564 1108 10-11 0.22995342595948642 1108 12-13 0.5130243355401518 1108 14-15 0.5144152182667199 1108 16-17 0.25279056339736883 1108 18-19 0.3268690291708012 1108 20-21 0.12720767313425796 1108 22-23 0.3246205869931629 1108 24-25 0.18166930484206034 1108 26-27 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2316 44-45 -0.5851845129148359 2316 46-47 -0.6786236669019416 2316 48-49 -0.571119505331211 2316 50-51 -0.5001803616353371 2316 52-53 -0.6719455307932236 2316 54-55 -0.23261819605386336 2316 56-57 -0.023549236796839068 2316 58-59 -0.35088913317958514 2316 60-61 -0.10044962494726661 2316 62-63 0.10346733944288644 2316 64-65 -0.01199909027050694 2316 66-67 0.307618452279975 2316 68-69 0.3241291914260671 2316 70-71 0.35667879000530434 2316 72-73 0.10232396160026269 2316 74-75 0.4904710389217968 2316 76-77 0.5813949391347073 2316 78-79 0.4723371782023378 2316 80-81 0.5792909322691848 2316 82-83 0.6052449412558261 2316 84-85 0.785559587581453 2316 86-87 0.8457486397530865 2316 88-89 1.210504458425035 2316 90-91 1.1897046228796242 2316 92-93 1.0012441461952797 2316 94-95 1.4917708025350898 2316 96-97 1.6166141564811518 2316 98-99 1.819078492224218 2316 100-101 2.156691499514203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 6.0 5 18.0 6 58.0 7 161.0 8 316.0 9 592.0 10 1003.0 11 1625.0 12 2194.0 13 3134.0 14 3956.0 15 5366.0 16 6796.0 17 8424.0 18 7820.0 19 6551.0 20 6345.0 21 6680.0 22 7269.0 23 7827.0 24 8828.0 25 10307.0 26 11650.0 27 13983.0 28 16745.0 29 20120.0 30 24425.0 31 29056.0 32 35566.0 33 43764.0 34 55119.0 35 71363.0 36 94714.0 37 125507.0 38 90689.0 39 6524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05274271656304 21.70810704838198 15.601306450976093 22.637843784078886 2 15.232140789626028 22.523343481192406 39.66709573721812 22.577419991963442 3 18.433785789165565 25.480660576438712 32.010047514669644 24.075506119726075 4 12.318688649575908 19.415528719826824 38.35005650025187 29.915726130345398 5 12.363950074822348 38.533372947836966 35.94566452875549 13.15701244858519 6 29.28796068127051 38.554274278090155 18.15836407945433 13.999400961185007 7 25.38522280159562 34.03207580563913 22.511334086670026 18.07136730609522 8 23.67020190330969 36.061455936610805 21.456889627098338 18.81145253298117 9 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30.565855352055628 20.17814658121258 34-35 20.498100855176844 29.15444312743776 30.46604108470819 19.881414932677206 36-37 20.22497227670065 28.774436156764448 30.749955241082084 20.250636325452813 38-39 19.8213574518928 29.011713205183632 30.85226247655487 20.314666866368704 40-41 20.286044749549163 28.918649003935514 30.66685909685343 20.128447149661895 42-43 20.199978624289205 28.795805459247614 30.42097215915782 20.583243757305354 44-45 20.32406093195088 28.766364212519818 30.2586891747556 20.650885680773705 46-47 20.507086702078052 28.710792712687855 29.975650417047024 20.80647016818707 48-49 20.265959619338062 28.572790023280824 30.242134211923595 20.919116145457515 50-51 20.02545914963717 28.85501899225334 30.20768948006154 20.91183237804795 52-53 20.17916706375679 29.074076595281205 29.826891396985744 20.919864943976258 54-55 20.269499394153925 28.623776395147786 29.698234196947627 21.408490013750665 56-57 20.563981429796733 28.74617091666553 29.302664361274864 21.38718329226287 58-59 20.445739336428367 28.855699718179466 29.41866005908701 21.279900886305157 60-61 20.345332262324543 28.96686226191611 29.460116267988184 21.227689207771167 62-63 19.99503070073927 29.018665504894418 29.378497229445482 21.607806564920832 64-65 19.92689003553389 29.207362731617 29.066452464908576 21.799294767940534 66-67 20.13118958978747 28.90071537536274 28.82658427154448 22.14151076330531 68-69 20.097561706145395 28.762868281485808 28.93005468275087 22.20951532961793 70-71 20.197955099317912 28.481640821772338 28.895317967080093 22.425086111829657 72-73 20.188629154129963 28.132564566854096 29.1796571864236 22.49914909259234 74-75 20.191828565982764 28.530040435120014 28.58027800846823 22.697852990428995 76-77 20.06378401927816 28.226028236511414 28.943853725612996 22.76633401859743 78-79 19.98223305332807 28.188792528352234 28.89307157152387 22.935902846795823 80-81 20.019877197042927 28.058569658684018 28.822820655947503 23.09873248832555 82-83 19.999782166369073 27.979640724269593 29.03633805328891 22.98423905607242 84-85 20.056339293798253 27.966781873124948 28.786547189604594 23.190331643472206 86-87 19.984479343231158 27.96815542440147 28.697490146425142 23.349875085942234 88-89 20.135600604484623 27.825080666022245 28.296619515050853 23.742699214442283 90-91 20.152265189759678 27.526861518186323 28.468920692490755 23.851952599563244 92-93 20.111666432950447 27.534516255076518 28.58079342799067 23.77302388398236 94-95 20.03818872445576 27.59009407632299 28.399613347673956 23.972103851547292 96-97 19.91960626812433 27.549931246681464 28.419558617309498 24.110903867884712 98-99 20.155682019305388 27.471715837769395 28.000299519407495 24.37230262351772 100-101 19.870021041424582 27.20095955975703 28.367529902852745 24.561489495965642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 670.0 1 395.0 2 133.5 3 144.0 4 112.0 5 117.0 6 192.0 7 346.0 8 492.0 9 519.5 10 443.0 11 364.5 12 365.5 13 414.5 14 473.0 15 536.0 16 612.0 17 724.0 18 864.0 19 994.5 20 1164.0 21 1432.0 22 1835.0 23 2309.5 24 2900.5 25 3661.5 26 4680.5 27 6072.5 28 7710.0 29 9548.5 30 11674.5 31 13832.5 32 15966.0 33 18241.5 34 20539.5 35 22612.0 36 24514.0 37 25878.5 38 26630.5 39 27228.0 40 27962.5 41 28844.0 42 29407.0 43 29897.5 44 30279.0 45 30209.0 46 30179.5 47 31476.0 48 33951.5 49 33040.0 50 29178.5 51 25933.5 52 22824.0 53 19432.5 54 16371.0 55 13904.0 56 11341.5 57 9027.5 58 7041.5 59 5194.0 60 3786.0 61 2704.5 62 1827.5 63 1233.5 64 798.0 65 506.5 66 332.0 67 253.0 68 201.0 69 136.0 70 78.0 71 48.0 72 31.0 73 23.5 74 19.5 75 16.0 76 5.5 77 2.0 78 2.5 79 2.0 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.3614518522552449E-4 2 0.049692992607316445 3 0.0 4 0.0 5 0.10510408299410491 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.16871111353147E-4 26-27 0.011095832595880246 28-29 0.0 30-31 0.0 32-33 3.4036296306381124E-4 34-35 0.013682591115165213 36-37 0.002654831111897728 38-39 0.011231977781105771 40-41 0.00605846074253584 42-43 0.004288573334604022 44-45 0.002518685926672203 46-47 6.807259261276224E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.807259261276224E-5 68-69 6.807259261276224E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.126533335148603E-4 84-85 0.004288573334604022 86-87 6.807259261276225E-4 88-89 0.0 90-91 2.7229037045104897E-4 92-93 1.3614518522552449E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.849437039659092E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 734510.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.29186377828944 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58392846230491 50.7689135322938 2 14.445910361372736 19.152926436590025 3 4.782326828517707 9.510880759780644 4 1.9275771471739072 5.111307266503857 5 0.953211756932206 3.1595091971206872 6 0.4677242425028244 1.860378706188051 7 0.2738893515846708 1.2709644909902515 8 0.1556478515934801 0.8254548940174714 9 0.09557484363744753 0.5702251063540485 >10 0.28834964935211116 2.9877674088795008 >50 0.012929975352288237 0.6029953576064621 >100 0.009030147716666014 1.1384765208987477 >500 0.0022575369236659502 0.9345357566075259 >1k 0.0016418450353934183 2.1056645661689735 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.4374344801296102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2978 0.405440361601612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2090 0.28454343712134617 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1964 0.2673891437829301 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1775 0.241657703775306 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1308 0.17807790227498604 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1131 0.15398020449006822 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1018 0.13859579855958395 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 973 0.13246926522443533 RNA PCR Primer, Index 23 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 770 0.10483179262365386 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.361451852255245E-4 0.0 16-17 0.0 0.0 0.0 2.72290370451049E-4 0.0 18-19 0.0 0.0 0.0 2.72290370451049E-4 0.0 20-21 0.0 0.0 0.0 9.530162965786714E-4 0.0 22-23 0.0 0.0 0.0 0.0025867585192849655 0.0 24-25 0.0 0.0 0.0 0.004628936297667833 0.0 26-27 0.0 0.0 0.0 0.009734380743625003 0.0 28-29 0.0 0.0 0.0 0.026548311118977277 0.0 30-31 0.0 0.0 0.0 0.07045513335420893 0.0 32-33 0.0 0.0 0.0 0.15683925337980423 0.0 34-35 0.0 0.0 0.0 0.30319532749724304 0.0 36-37 0.0 0.0 0.0 0.5204830431171802 0.0 38-39 0.0 0.0 0.0 0.7699010224503411 0.0 40-41 0.0 0.0 0.0 1.0043430314086943 0.0 42-43 0.0 0.0 0.0 1.2481790581476087 0.0 44-45 0.0 0.0 0.0 1.5614491293515405 0.0 46-47 0.0 0.0 0.0 1.9188302405685422 0.0 48-49 0.0 0.0 0.0 2.257695606594873 0.0 50-51 0.0 0.0 0.0 2.5781813726157576 0.0 52-53 0.0 0.0 0.0 2.8862098541885066 0.0 54-55 0.0 0.0 0.0 3.259383806891669 0.0 56-57 0.0 0.0 0.0 3.7179888633238485 0.0 58-59 0.0 0.0 0.0 4.164613143456181 0.0 60-61 0.0 0.0 0.0 4.61014826210671 0.0 62-63 0.0 0.0 0.0 5.048195395569836 0.0 64-65 0.0 0.0 0.0 5.539134933493077 0.0 66-67 0.0 0.0 0.0 6.1050904684755825 0.0 68-69 0.0 0.0 0.0 6.727410110141455 0.0 70-71 0.0 0.0 0.0 7.369130440701964 0.0 72-73 0.0 0.0 0.0 7.98042232236457 0.0 74-75 0.0 0.0 0.0 8.653660263304788 0.0 76-77 0.0 0.0 0.0 9.376318906481872 0.0 78-79 0.0 0.0 0.0 10.16705014227172 0.0 80-81 0.0 0.0 0.0 10.9798368980681 0.0 82-83 0.0 0.0 0.0 11.794597759050252 0.0 84-85 0.0 0.0 0.0 12.616097806701067 0.0 86-87 0.0 0.0 0.0 13.491851710664253 0.0 88-89 0.0 0.0 0.0 14.422676342051163 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGCGT 100 4.954725E-5 28.496609 9 CAGCGTC 70 0.00832504 27.185894 2 GGGAGCG 105 6.90809E-5 27.139627 8 AGGACGT 160 1.8402225E-6 23.776306 5 GAGCGTA 70 1.7815582E-5 23.747175 10-11 CTCCGAC 155 0.0 22.981136 94-95 GTACATG 2100 0.0 22.428362 1 GGACGTG 130 2.9424747E-4 21.92047 6 TAGGACG 155 3.870756E-5 21.475374 4 GTATAGC 425 0.0 21.268963 1 TACATGG 2085 0.0 21.220644 2 AGTACTT 2800 0.0 21.033213 12-13 AGCGTAC 70 4.8565166E-4 20.354721 10-11 GTACGCG 70 4.8565166E-4 20.354721 14-15 ACATGGG 2135 0.0 19.60014 3 GAGTACT 2935 0.0 19.499384 12-13 CATGGGG 900 0.0 19.021048 4 ACGCGTT 75 7.728634E-4 18.99774 16-17 AGAGTAC 4210 0.0 18.839802 10-11 ATAGCCC 430 0.0 18.799873 3 >>END_MODULE