FastQCFastQC Report
Thu 26 May 2016
SRR938560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732972
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.44585604907145154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.39373946071609833No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19970.2724524265592683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.2637208515468531No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12460.16999285102295858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11050.15075609982373134No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8800.12005915642070912No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.1036874532724306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGT452.3981027E-531.69616394-95
CATTCCG753.0771995E-431.6669629
TATAGCG600.00394984531.6669627
CGCCCAT300.003951123431.66696290-91
GCCGTAT1400.025.4701394-95
GTAAGCG951.1797965E-825.02328594-95
TCGCGGT550.00287758421.5911192-93
GAGTACT28100.020.79271712-13
CCGTATC1158.936513E-820.6714194-95
CGCTGCG704.8222416E-420.37610494-95
GTACTTT29650.019.0655614-15
TAGGACG1759.909016E-519.0053654
GTATAGC3901.4551915E-1118.3344611
GGTATCA51250.018.323731
CATGGGG9450.018.1003484
TCGCCGT1455.2152245E-818.01740892-93
GTCCAGG3451.029548E-917.9624581
GTACATG23500.017.8507441
AGTACTT27700.017.66322312-13
ATAGCCC4353.6379788E-1217.4761983
Produced by FastQC (version 0.11.5)