##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938560_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732972 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14875875203964 31.0 31.0 34.0 28.0 34.0 2 31.328625104369607 31.0 31.0 34.0 28.0 34.0 3 31.38114962099507 33.0 31.0 34.0 28.0 34.0 4 35.01616296393314 37.0 35.0 37.0 32.0 37.0 5 35.03312814132054 37.0 35.0 37.0 32.0 37.0 6 35.011020884835986 37.0 35.0 37.0 32.0 37.0 7 34.95363806530127 37.0 35.0 37.0 32.0 37.0 8 34.93491702275121 37.0 35.0 37.0 32.0 37.0 9 36.450280501847274 39.0 37.0 39.0 32.0 39.0 10-11 36.3170086988316 39.0 37.0 39.0 32.0 39.0 12-13 36.18986605218207 39.0 35.0 39.0 32.0 39.0 14-15 37.361474926736626 40.0 37.0 41.0 32.0 41.0 16-17 37.30565424054397 40.0 37.0 41.0 32.0 41.0 18-19 37.332241204302484 40.0 37.0 41.0 32.0 41.0 20-21 37.30494207145703 40.0 37.0 41.0 32.0 41.0 22-23 37.18182959239917 40.0 37.0 41.0 31.0 41.0 24-25 37.080713860829604 39.0 36.0 41.0 31.0 41.0 26-27 36.89682211598806 39.0 36.0 41.0 31.0 41.0 28-29 36.69755256681019 39.0 36.0 41.0 30.0 41.0 30-31 36.467781852512786 39.0 36.0 40.0 30.0 41.0 32-33 36.196605736644784 39.0 35.0 40.0 30.0 41.0 34-35 35.93860474888536 38.0 35.0 40.0 29.0 41.0 36-37 35.76071527971055 38.0 35.0 40.0 28.5 41.0 38-39 35.578427279623234 38.0 35.0 40.0 27.0 41.0 40-41 35.35903827158472 38.0 35.0 40.0 27.0 41.0 42-43 35.01607360172012 38.0 34.0 40.0 25.5 41.0 44-45 34.58693033294587 38.0 33.5 40.0 24.0 41.0 46-47 34.14660109799556 38.0 33.0 40.0 23.5 41.0 48-49 33.72411633732257 38.0 33.0 40.0 20.0 41.0 50-51 33.03352924804767 37.5 32.0 39.5 18.5 40.5 52-53 33.09198100336711 37.5 33.0 39.5 18.0 40.0 54-55 33.1950804396348 38.0 33.0 40.0 16.0 41.0 56-57 32.87502592186332 38.0 32.0 40.0 11.0 41.0 58-59 32.54276016000611 37.0 31.0 40.0 8.5 41.0 60-61 32.10593856245532 37.0 31.0 39.5 7.5 41.0 62-63 31.50279546831257 36.0 30.0 39.0 2.0 40.0 64-65 30.976524341993965 36.0 29.0 39.0 2.0 40.0 66-67 30.396290717790038 35.0 28.5 38.5 2.0 40.0 68-69 29.66737474282783 34.5 28.0 38.0 2.0 40.0 70-71 28.94048271966733 34.0 26.0 38.0 2.0 39.5 72-73 28.13133653127268 34.0 26.0 37.0 2.0 39.0 74-75 27.27892811730871 33.0 24.0 36.0 2.0 39.0 76-77 26.41470888383185 32.0 21.5 35.5 2.0 38.5 78-79 25.47370977336106 31.0 19.0 35.0 2.0 37.5 80-81 24.543681068308203 31.0 11.0 34.5 2.0 36.5 82-83 23.575725266449467 30.0 4.5 34.0 2.0 36.0 84-85 22.60215874549096 29.0 2.0 34.0 2.0 35.5 86-87 21.628197939348297 29.0 2.0 34.0 2.0 35.0 88-89 20.536030025703575 27.0 2.0 33.5 2.0 35.0 90-91 19.34496269980299 26.0 2.0 32.5 2.0 34.5 92-93 18.15166541150276 24.5 2.0 32.0 2.0 34.0 94-95 16.825408746855267 19.0 2.0 31.5 2.0 34.0 96-97 15.287786573020526 2.0 2.0 31.0 2.0 34.0 98-99 13.406075539038326 2.0 2.0 31.0 2.0 34.0 100-101 9.408639757043925 2.0 2.0 22.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43209892087731916 1101 2 0.5075986624996034 1101 3 0.4500655355840273 1101 4 0.46486727510043124 1101 5 0.33316283171425454 1101 6 0.4813083046830684 1101 7 0.414367830524597 1101 8 0.4563769571119636 1101 9 0.3572692840734746 1101 10-11 0.5398547772434839 1101 12-13 0.429922046774756 1101 14-15 0.5209930106444602 1101 16-17 0.6131517383326965 1101 18-19 0.3218262671798158 1101 20-21 0.5396623963134317 1101 22-23 0.5501425734991656 1101 24-25 0.6733144937848579 1101 26-27 0.6204453512906838 1101 28-29 0.842537933191295 1101 30-31 0.7996869821029904 1101 32-33 0.49982076150543264 1101 34-35 0.5595419581552221 1101 36-37 0.8645260393480001 1101 38-39 0.8242747450042316 1101 40-41 1.070308399024995 1101 42-43 1.2148986903402275 1101 44-45 1.310213008749301 1101 46-47 1.4573653691788877 1101 48-49 1.508385327533361 1101 50-51 1.4969064943616885 1101 52-53 1.2896973724286696 1101 54-55 1.1942094857013146 1101 56-57 1.5870581428371224 1101 58-59 1.795957612047971 1101 60-61 1.9226110415310131 1101 62-63 1.6286719008777375 1101 64-65 1.7275413060888027 1101 66-67 1.836294942984491 1101 68-69 1.915846433282649 1101 70-71 2.3824500536291175 1101 72-73 2.542649733153855 1101 74-75 2.2428947529746637 1101 76-77 2.7521393524207127 1101 78-79 2.7160670292308886 1101 80-81 2.8894812271344072 1101 82-83 3.07115902094365 1101 84-85 3.452802459989993 1101 86-87 3.6454007980467082 1101 88-89 3.6188114788959638 1101 90-91 3.484740460258795 1101 92-93 3.7914622176944697 1101 94-95 3.849655543197059 1101 96-97 3.9315621973513757 1101 98-99 3.495310080179463 1101 100-101 2.732944095643555 1102 1 0.5354028410011118 1102 2 0.64195224734965 1102 3 0.5404881278764151 1102 4 0.5300015688149742 1102 5 0.5503631117231009 1102 6 0.5099124974470541 1102 7 0.6268153288666554 1102 8 0.42564967098714135 1102 9 0.5460311660276389 1102 10-11 0.7434460748634066 1102 12-13 0.7066034814516442 1102 14-15 0.7468906047790114 1102 16-17 0.8492641287239664 1102 18-19 0.7999068249869055 1102 20-21 0.6608652369294532 1102 22-23 0.44950288224575985 1102 24-25 0.7243769615469944 1102 26-27 0.598931185053857 1102 28-29 0.48329303598401196 1102 30-31 0.3831024452228817 1102 32-33 0.45321402655590504 1102 34-35 0.688447344641105 1102 36-37 0.6320887650130302 1102 38-39 0.6927419729166928 1102 40-41 0.8767661029524874 1102 42-43 0.9679543789409166 1102 44-45 1.0286889869380929 1102 46-47 1.114863434907278 1102 48-49 1.0507297568311316 1102 50-51 1.0314079128275253 1102 52-53 1.2636261543902094 1102 54-55 1.1786915614510818 1102 56-57 1.4355217785308838 1102 58-59 1.5701360071551846 1102 60-61 1.353083188019852 1102 62-63 1.6764806474697345 1102 64-65 2.1313757692823714 1102 66-67 2.0758936276797932 1102 68-69 2.184758543665083 1102 70-71 1.9724420402181657 1102 72-73 1.89288645115548 1102 74-75 1.8580529948138782 1102 76-77 2.124410647830043 1102 78-79 2.64183935625174 1102 80-81 2.7759924459097363 1102 82-83 2.8786068877183872 1102 84-85 2.9610507467779996 1102 86-87 3.7324199933897226 1102 88-89 3.604251479632829 1102 90-91 3.6842997226565544 1102 92-93 3.804157026477906 1102 94-95 3.7242157266765368 1102 96-97 3.4433635568679932 1102 98-99 2.98784010744348 1102 100-101 2.708406478666177 1103 1 0.6777599381204951 1103 2 0.6134017537808703 1103 3 0.5549513906303467 1103 4 0.6191082458529564 1103 5 0.574502213458004 1103 6 0.6804526512118869 1103 7 0.6725687464597883 1103 8 0.5782240930797684 1103 9 0.6368181686448224 1103 10-11 0.6260424572212742 1103 12-13 0.6417916908090362 1103 14-15 0.7404282273117104 1103 16-17 0.8889698783597524 1103 18-19 1.1711620840621606 1103 20-21 0.8793032647171373 1103 22-23 0.9630625939689352 1103 24-25 0.8478658503403835 1103 26-27 0.784885425190005 1103 28-29 1.1127973614256845 1103 30-31 0.8509882432486009 1103 32-33 0.9861597963040296 1103 34-35 1.0114770447180348 1103 36-37 1.0809666857068194 1103 38-39 1.0687008543613459 1103 40-41 1.5005182141032307 1103 42-43 1.3634465080552403 1103 44-45 1.312266490343859 1103 46-47 1.6397728649288013 1103 48-49 1.6520563975122045 1103 50-51 1.5513107461744724 1103 52-53 1.5383507915731016 1103 54-55 1.5593529908881507 1103 56-57 1.902040969052301 1103 58-59 1.658519628493174 1103 60-61 2.1193180983953255 1103 62-63 2.239392939631518 1103 64-65 2.3234174293237118 1103 66-67 2.671494913494147 1103 68-69 2.7462000137565745 1103 70-71 2.467682550246405 1103 72-73 2.797335661058657 1103 74-75 2.5528837374662174 1103 76-77 3.1422173717108635 1103 78-79 3.1102064751594867 1103 80-81 3.3246187475174374 1103 82-83 3.258791293781311 1103 84-85 3.5164907471218 1103 86-87 3.5268648198348664 1103 88-89 3.687915362946896 1103 90-91 3.87985352100889 1103 92-93 3.682995238198931 1103 94-95 3.8680447202431694 1103 96-97 3.839501386540565 1103 98-99 3.44186751694871 1103 100-101 2.7461106640090964 1104 1 0.724262410140863 1104 2 0.757795836759243 1104 3 0.7338310975094657 1104 4 0.596645405294808 1104 5 0.5817847320500888 1104 6 0.7049908414041255 1104 7 0.6552893339677297 1104 8 0.67531247052829 1104 9 0.7189992443842002 1104 10-11 0.7528771830238625 1104 12-13 0.9392581419611545 1104 14-15 0.9454911703513744 1104 16-17 0.9590727753420865 1104 18-19 0.919723287092296 1104 20-21 1.0239891628251883 1104 22-23 1.0000083677934128 1104 24-25 0.9816388518024297 1104 26-27 0.9680879528036215 1104 28-29 1.2119937322346814 1104 30-31 0.9802445400255806 1104 32-33 1.195629642403695 1104 34-35 0.7894047418317314 1104 36-37 1.1973890520010784 1104 38-39 1.2685942181885324 1104 40-41 1.0952562432530897 1104 42-43 1.0869294156022775 1104 44-45 0.9682471177760732 1104 46-47 1.1580177403583747 1104 48-49 1.050229733720819 1104 50-51 0.9639709724647432 1104 52-53 1.164911507914944 1104 54-55 1.5247170080053323 1104 56-57 1.3532900367276852 1104 58-59 1.349593250977378 1104 60-61 1.797036479978729 1104 62-63 1.9389202980418183 1104 64-65 2.1340828271716106 1104 66-67 2.2353915456507956 1104 68-69 2.3740635115417525 1104 70-71 2.6018997042278507 1104 72-73 2.937636418590948 1104 74-75 2.931649883745724 1104 76-77 3.015571583839691 1104 78-79 2.6806615287050874 1104 80-81 2.9630410069776865 1104 82-83 3.3059575215712123 1104 84-85 3.4525713632770803 1104 86-87 3.3458464542765682 1104 88-89 3.176590957507859 1104 90-91 2.8670581694755235 1104 92-93 2.825525661162704 1104 94-95 3.2277053837615277 1104 96-97 3.4152635186309297 1104 98-99 3.3938861852033764 1104 100-101 2.389326605226648 1105 1 0.5880292906648883 1105 2 0.399825911384994 1105 3 0.38905714324204155 1105 4 0.3462434880561105 1105 5 0.39302383355008175 1105 6 0.4571967127606982 1105 7 0.3570966529741142 1105 8 0.38064918370519507 1105 9 0.5979754209735475 1105 10-11 0.4946967651794907 1105 12-13 0.455762920102849 1105 14-15 0.47190928392009823 1105 16-17 0.6702671986001931 1105 18-19 0.7091607151210653 1105 20-21 0.49839731632392414 1105 22-23 0.4764719073570305 1105 24-25 0.6694379530033245 1105 26-27 0.6210436334831826 1105 28-29 0.4383778230418045 1105 30-31 0.4514457678360131 1105 32-33 0.4912808978809906 1105 34-35 0.5065652993563745 1105 36-37 0.7041618248187618 1105 38-39 0.930777170915583 1105 40-41 1.1442331302077946 1105 42-43 1.1447849347671806 1105 44-45 1.344159376438789 1105 46-47 1.2607272233619398 1105 48-49 1.4928873600536932 1105 50-51 1.253831815631024 1105 52-53 1.2491944875611125 1105 54-55 1.5593501229947861 1105 56-57 1.938782506854956 1105 58-59 1.6088018291998196 1105 60-61 1.6781185159606054 1105 62-63 1.7920111350765318 1105 64-65 1.9566587988766315 1105 66-67 2.14701648769994 1105 68-69 2.1825901390261606 1105 70-71 2.336431498685503 1105 72-73 2.469708479547773 1105 74-75 2.5521820595574063 1105 76-77 2.551206687852133 1105 78-79 2.7756084008542814 1105 80-81 2.7758158062060048 1105 82-83 3.0480145153050415 1105 84-85 3.0785892420513647 1105 86-87 2.83522836164493 1105 88-89 2.9626270440677445 1105 90-91 2.865010260940675 1105 92-93 3.0281442921765063 1105 94-95 2.9839248193575614 1105 96-97 3.3505810145556794 1105 98-99 3.1213919055138444 1105 100-101 2.1956539041932537 1106 1 0.2695398077363862 1106 2 0.18519518697082304 1106 3 0.1192546328534867 1106 4 0.3857467652698361 1106 5 0.29759372602320155 1106 6 0.05961499661453473 1106 7 0.25613490855394616 1106 8 0.2454159231988342 1106 9 0.5438906846500302 1106 10-11 0.4576085588649619 1106 12-13 0.5434283872664523 1106 14-15 0.5446291571154944 1106 16-17 0.19741934271191752 1106 18-19 0.7304063776323133 1106 20-21 0.7214727564987129 1106 22-23 0.4727903968399545 1106 24-25 0.3765958804333849 1106 26-27 0.5780569779547591 1106 28-29 0.5129558549999942 1106 30-31 0.4042554966626355 1106 32-33 0.46013398017546336 1106 34-35 0.40527690454850784 1106 36-37 0.6718020167392567 1106 38-39 0.6647472047587044 1106 40-41 0.5989438571923245 1106 42-43 0.39699651853962337 1106 44-45 0.8556349794032769 1106 46-47 0.9103491657001186 1106 48-49 0.6734152484999001 1106 50-51 0.5837754818264571 1106 52-53 0.9862141508434732 1106 54-55 0.5670585366519916 1106 56-57 1.1534025214203467 1106 58-59 1.0552740050126985 1106 60-61 1.4021016158192339 1106 62-63 1.09380931868618 1106 64-65 0.7577153254584772 1106 66-67 1.2447920521089664 1106 68-69 1.6771174063011465 1106 70-71 1.4327478461986765 1106 72-73 1.4842390668758796 1106 74-75 1.7883288701644418 1106 76-77 1.799843859595267 1106 78-79 1.9485912008371429 1106 80-81 1.8237289921718194 1106 82-83 2.3159481570820226 1106 84-85 2.519569942903864 1106 86-87 2.1240087240662078 1106 88-89 1.9502732335035518 1106 90-91 2.0243594234305 1106 92-93 2.5171559873313676 1106 94-95 2.235558071296044 1106 96-97 2.2459297272318537 1106 98-99 2.1724511095578833 1106 100-101 1.868920515168707 1107 1 0.05805755804101054 1107 2 0.03316755865248311 1107 3 0.023770589644879436 1107 4 0.04911733796188855 1107 5 -0.052492184629720384 1107 6 0.010586026584000763 1107 7 0.17442566306169027 1107 8 0.1795351165286121 1107 9 0.3291859297863198 1107 10-11 0.3214898036452851 1107 12-13 0.20796611265355835 1107 14-15 0.25151381778552206 1107 16-17 0.3497858218127021 1107 18-19 0.28224712461003776 1107 20-21 0.29510776971046937 1107 22-23 0.286650934072469 1107 24-25 0.3686328870461111 1107 26-27 0.49757478302314695 1107 28-29 0.5971739653763564 1107 30-31 0.47677555389076787 1107 32-33 0.5010151291192102 1107 34-35 0.3220513873732216 1107 36-37 0.5733282143542908 1107 38-39 0.49116404598486696 1107 40-41 0.5844836567569587 1107 42-43 0.8662612123417333 1107 44-45 0.4547000592899124 1107 46-47 0.5859406143385542 1107 48-49 0.607792858335479 1107 50-51 1.0430389434439107 1107 52-53 0.8964204048651752 1107 54-55 0.8862747987513515 1107 56-57 1.183267597199709 1107 58-59 1.1221456935164156 1107 60-61 1.1738368398714698 1107 62-63 1.1563623710814035 1107 64-65 1.2588148791338085 1107 66-67 1.5480044602085279 1107 68-69 1.505919038261279 1107 70-71 1.6730349794526056 1107 72-73 1.3224617774083214 1107 74-75 1.4126049616746919 1107 76-77 1.4220774338891538 1107 78-79 1.8452237428341078 1107 80-81 2.1026365577856545 1107 82-83 2.223362314883804 1107 84-85 2.2114459098265513 1107 86-87 2.3188984093114833 1107 88-89 2.4309578832208345 1107 90-91 2.2542825145710914 1107 92-93 2.3830592129170007 1107 94-95 2.1121256840067133 1107 96-97 2.1812400324029255 1107 98-99 1.8581935350449381 1107 100-101 1.5752404245346785 1108 1 0.23853980606170921 1108 2 0.16902447678927146 1108 3 0.19739352746420025 1108 4 0.061607425194246446 1108 5 0.24420995132794587 1108 6 0.27959091471598185 1108 7 0.14465648429386135 1108 8 0.2767257406754595 1108 9 0.36600617393290946 1108 10-11 0.09376590510002814 1108 12-13 0.37492962066827573 1108 14-15 0.19996661351100897 1108 16-17 0.3937270082247366 1108 18-19 0.3204681565779026 1108 20-21 0.03438085508376787 1108 22-23 0.0727537213477234 1108 24-25 0.04060315524335323 1108 26-27 0.13805657603155908 1108 28-29 -0.014724930893841304 1108 30-31 0.24468109984051267 1108 32-33 0.23196636480257382 1108 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-0.3473726639856132 2316 94-95 -0.5380975131486423 2316 96-97 0.31718564648288883 2316 98-99 0.17691299732525856 2316 100-101 0.15589997532642208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4829.0 3 979.0 4 773.0 5 780.0 6 882.0 7 1083.0 8 1335.0 9 1732.0 10 2227.0 11 2877.0 12 3950.0 13 5105.0 14 6128.0 15 7526.0 16 8975.0 17 10761.0 18 10349.0 19 9433.0 20 10264.0 21 11238.0 22 12210.0 23 13820.0 24 15610.0 25 17474.0 26 20096.0 27 22900.0 28 26092.0 29 29601.0 30 33733.0 31 38910.0 32 44992.0 33 52450.0 34 61185.0 35 71403.0 36 79752.0 37 69559.0 38 21605.0 39 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.456301769352685 20.90019601638812 15.128191430344323 22.515310783914877 2 15.228341878895488 22.039225426789073 40.32240675056193 22.410025943753507 3 18.803935564614857 25.22700193666465 31.485472285496307 24.483590213224186 4 12.307646109790154 18.667165324022637 38.98631513142988 30.038873434757328 5 12.696432106265487 38.143493901065526 35.70706002006812 13.45301397260087 6 29.47539985557832 37.95450853630403 18.591219762564208 13.978871845553437 7 25.666018845057813 33.86105318324253 22.81578573846204 17.657142233237614 8 23.67086068040469 35.52150848015111 21.69149910401236 19.11613173543184 9 24.515854320940477 17.51672503254933 22.832978952798697 35.1344416937115 10-11 22.9815114191366 27.68066679814466 29.475204911180704 19.86261687153804 12-13 24.230092396418232 25.82111764115854 28.993342353942218 20.95544760848101 14-15 21.351726264923446 27.906954528737423 28.01742402066646 22.723895185672674 16-17 20.009320243145083 30.449493629688863 29.086800393278782 20.45438573388727 18-19 19.535549234114765 29.569945665783496 31.20631786233103 19.68818723777071 20-21 21.051309906595115 28.446591076585452 30.873698691458173 19.628400325361255 22-23 20.32921928641177 28.8114300765558 30.408395412520377 20.45095522451205 24-25 20.322166118098913 28.791242388238565 30.84272427185394 20.043867221808586 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28.810266136349682 30.11440929464657 20.8630840707059 52-53 20.346130152196025 29.088618078642003 29.669253864935836 20.895997904226135 54-55 20.368896047503366 28.532650035849922 29.868613955341434 21.229839961305277 56-57 20.594555727319737 28.55302851482501 29.58977619566745 21.262639562187804 58-59 20.64243652993953 28.43068501822786 29.55921598375865 21.36766246807396 60-61 20.515701491559835 28.262957687290907 29.92745496122381 21.29388585992545 62-63 20.28341050451327 28.2597972832379 29.97996300968284 21.476829202565987 64-65 20.28795775800906 28.391434262948206 29.648665611526496 21.671942367516237 66-67 20.365142861820654 28.242667263345457 29.361303561244622 22.030886313589267 68-69 20.351041636820828 28.114029522480227 29.588448057325838 21.946480783373104 70-71 20.46620246998481 27.837064508602378 29.58912726384574 22.107605757567075 72-73 20.469504707327058 27.36464729158139 29.999522445081183 22.16632555601037 74-75 20.425263666338513 27.855813963007915 29.431091990369584 22.28783038028399 76-77 20.393253981248023 27.473885850879203 29.599924140716244 22.532936027156534 78-79 20.382148658393238 27.50438833769836 29.541477551426954 22.57198545248145 80-81 20.525966419633825 27.393424873976407 29.45387389931894 22.62673480707083 82-83 20.525637859352337 27.3066159444712 29.614766926690688 22.552979269485768 84-85 20.67841751698534 27.14850292293135 29.46801899001448 22.705060570068827 86-87 20.64999542948514 27.04776119810249 29.457309344724603 22.84493402768777 88-89 20.904251218342374 26.901367995682673 29.2877703191808 22.90661046679416 90-91 21.00135646696307 26.702884607511606 29.24223443133466 23.053524494190665 92-93 21.18154951139443 26.53792730815307 29.354265645338668 22.926257535113837 94-95 21.090480859306503 26.445317324021406 29.234870333716103 23.229331482955992 96-97 21.007590359390722 26.309311409418868 29.196650007675647 23.486448223514763 98-99 21.358385426651267 26.132914540124535 28.79910547080466 23.70959456241954 100-101 21.99496229232091 25.145100460469433 29.301140997078186 23.558796250131476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 547.0 1 344.0 2 147.5 3 161.5 4 133.0 5 110.5 6 177.0 7 307.0 8 427.5 9 465.5 10 414.5 11 366.0 12 369.5 13 402.5 14 456.5 15 507.5 16 582.0 17 699.0 18 831.0 19 1007.0 20 1226.0 21 1497.0 22 1860.5 23 2366.5 24 3024.0 25 3792.5 26 4855.0 27 6249.0 28 7769.0 29 9613.0 30 11642.0 31 13788.5 32 16059.5 33 18234.0 34 20602.0 35 22574.0 36 24154.0 37 25796.0 38 26817.0 39 27184.0 40 27698.5 41 28590.5 42 29423.5 43 29767.0 44 29907.0 45 29882.0 46 30008.5 47 30817.0 48 32566.0 49 32193.5 50 28561.5 51 25069.5 52 22043.0 53 18929.5 54 16232.0 55 13880.0 56 11593.5 57 9371.5 58 7384.5 59 5745.5 60 4339.0 61 3257.0 62 2374.0 63 1649.5 64 1131.5 65 814.0 66 603.0 67 473.5 68 362.5 69 268.5 70 206.5 71 138.5 72 102.5 73 80.5 74 59.0 75 45.5 76 34.0 77 23.5 78 18.5 79 13.0 80 11.0 81 7.5 82 2.5 83 1.5 84 1.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39987884939670276 2 0.03178839027957412 3 0.036699901224057675 4 0.046113630534317815 5 0.06371321141871722 6 0.05389018952975011 7 0.03547202348793679 8 0.03560845434750577 9 0.032470544577419054 10-11 0.03772313267082508 12-13 0.02803654164142696 14-15 0.025990078747892144 16-17 0.02162429124168454 18-19 0.026262940467030118 20-21 0.03383485317310893 22-23 0.03253876000720355 24-25 0.04181605845789471 26-27 0.03594953149642824 28-29 0.030696943403022217 30-31 0.03369842231353994 32-33 0.029264419377547847 34-35 0.030287650824315254 36-37 0.032675190866772535 38-39 0.014257024824959207 40-41 0.011733053922932937 42-43 0.012278777361208887 44-45 0.010436960757027554 46-47 0.019032104909873775 48-49 0.016371703148278516 50-51 0.010505176186812048 52-53 0.009345513880475652 54-55 0.0075719127060788136 56-57 0.00750369727629432 58-59 0.003820064067931654 60-61 0.0065486812593114055 62-63 0.010573391616596541 64-65 0.007094404697587356 66-67 0.003547202348793678 68-69 0.005320803523190518 70-71 0.01268806993991585 72-73 0.00982302188896711 74-75 0.011664838493148443 76-77 0.004911510944483555 78-79 0.008663359582630713 80-81 0.010027668178320591 82-83 0.012415208220777874 84-85 0.008254067003923752 86-87 0.001773601174396839 88-89 0.01521204084194212 90-91 0.0253761398798317 92-93 0.009550160169829134 94-95 0.020259982645994663 96-97 0.018349950612028838 98-99 0.009891237318751604 100-101 0.12183275759510596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 732972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.07823788996303 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6978405359983 54.363081499720664 2 13.370319483058177 18.471962198310006 3 4.278772734305468 8.867102424523223 4 1.7169587039335423 4.744179271902555 5 0.8373143946691509 2.892010147182301 6 0.40203082222369607 1.6662948465999547 7 0.2223681184724957 1.0752558450890581 8 0.12474493291800591 0.6893728121341995 9 0.08579191452175508 0.5333718852332255 >10 0.2432957272745676 2.675006067420538 >50 0.008897474441795443 0.4208084264852144 >100 0.008699440009793159 1.1565401665074 >500 0.001779430903931049 0.7466704152279654 >1k 0.0011862872692873661 1.6983439936637552 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3268 0.44585604907145154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2886 0.39373946071609833 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1997 0.2724524265592683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1933 0.2637208515468531 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1246 0.16999285102295858 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1105 0.15075609982373134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 880 0.12005915642070912 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 760 0.1036874532724306 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3643085956898762E-4 0.0 3 0.0 0.0 0.0 1.3643085956898762E-4 0.0 4 0.0 0.0 0.0 1.3643085956898762E-4 0.0 5 0.0 0.0 0.0 1.3643085956898762E-4 0.0 6 0.0 0.0 0.0 1.3643085956898762E-4 0.0 7 0.0 0.0 0.0 1.3643085956898762E-4 0.0 8 0.0 0.0 0.0 1.3643085956898762E-4 0.0 9 0.0 0.0 0.0 1.3643085956898762E-4 0.0 10-11 0.0 0.0 0.0 1.3643085956898762E-4 0.0 12-13 0.0 0.0 0.0 1.3643085956898762E-4 0.0 14-15 0.0 0.0 0.0 1.3643085956898762E-4 0.0 16-17 0.0 0.0 0.0 1.3643085956898762E-4 0.0 18-19 0.0 0.0 0.0 1.3643085956898762E-4 0.0 20-21 0.0 0.0 0.0 3.4107714892246904E-4 0.0 22-23 0.0 0.0 0.0 0.0015689548850433576 0.0 24-25 0.0 0.0 0.0 0.004570433795561085 0.0 26-27 0.0 0.0 0.0 0.01125554591444148 0.0 28-29 0.0 0.0 0.0 0.02878691136905639 0.0 30-31 0.0 0.0 0.0 0.07428660303531376 0.0 32-33 0.0 0.0 0.0 0.1643309703508456 0.0 34-35 0.0 0.0 0.0 0.30601441801323925 0.0 36-37 0.0 0.0 0.0 0.5115475079539191 0.0 38-39 0.0 0.0 0.0 0.7382273811277921 0.0 40-41 0.0 0.0 0.0 0.9603368205061038 0.0 42-43 0.0 0.0 0.0 1.1868802628204078 0.0 44-45 0.0 0.0 0.0 1.5029223490119676 0.0 46-47 0.0 0.0 0.0 1.8617355096784052 0.0 48-49 0.0 0.0 0.0 2.190192804090743 0.0 50-51 0.0 0.0 0.0 2.5069170445801476 0.0 52-53 0.0 0.0 0.0 2.8298488891799414 0.0 54-55 0.0 0.0 0.0 3.21342425085815 0.0 56-57 0.0 0.0 0.0 3.6669886434952494 0.0 58-59 0.0 0.0 0.0 4.128056733408643 0.0 60-61 0.0 0.0 0.0 4.584827251245613 0.0 62-63 0.0 0.0 0.0 5.025362496793875 0.0 64-65 0.0 0.0 0.0 5.5133756814721435 0.0 66-67 0.0 0.0 0.0 6.085566706504478 0.0 68-69 0.0 0.0 0.0 6.693775478463024 0.0 70-71 0.0 0.0 0.0 7.325450358267437 0.0 72-73 0.0 0.0 0.0 7.931135159323958 0.0 74-75 0.0 0.0 0.0 8.563901486004923 0.0 76-77 0.0 0.0 0.0 9.256424529177103 0.0 78-79 0.0 0.0 0.0 10.028418548048219 0.0 80-81 0.0 0.0 0.0 10.806551955599941 0.0 82-83 0.0 0.0 0.0 11.565107534803513 0.0 84-85 0.0 0.0 0.0 12.335191521640663 0.0 86-87 0.0 0.0 0.0 13.166601179854073 0.0 88-89 0.0 0.0 0.0 14.0106989080074 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGACGT 45 2.3981027E-5 31.696163 94-95 CATTCCG 75 3.0771995E-4 31.666962 9 TATAGCG 60 0.003949845 31.666962 7 CGCCCAT 30 0.0039511234 31.666962 90-91 GCCGTAT 140 0.0 25.47013 94-95 GTAAGCG 95 1.1797965E-8 25.023285 94-95 TCGCGGT 55 0.002877584 21.59111 92-93 GAGTACT 2810 0.0 20.792717 12-13 CCGTATC 115 8.936513E-8 20.67141 94-95 CGCTGCG 70 4.8222416E-4 20.376104 94-95 GTACTTT 2965 0.0 19.06556 14-15 TAGGACG 175 9.909016E-5 19.005365 4 GTATAGC 390 1.4551915E-11 18.334461 1 GGTATCA 5125 0.0 18.32373 1 CATGGGG 945 0.0 18.100348 4 TCGCCGT 145 5.2152245E-8 18.017408 92-93 GTCCAGG 345 1.029548E-9 17.962458 1 GTACATG 2350 0.0 17.850744 1 AGTACTT 2770 0.0 17.663223 12-13 ATAGCCC 435 3.6379788E-12 17.476198 3 >>END_MODULE