FastQCFastQC Report
Thu 26 May 2016
SRR938560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732972
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31160.42511855841696544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.41324907363446356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20300.2769546449250449No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19420.26494872928297397No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17670.24107332885840113No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13770.18786529362649595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.13820446074338447No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT9980.13615799784984967RNA PCR Primer, Index 23 (95% over 23bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9200.12551639080346863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGC250.005021536756.9971779
GCGGTAT600.00394439131.6759011
ATGCCGC404.4955424E-429.68603194-95
GGTTGCG650.00584412329.2293227
CTCCGAC3000.024.54045194-95
GGAGCGT1050.002196099822.6179289
GGGAGCG1100.00287720921.589848
GTGTAGA3351.8189894E-1221.274861
GTACATG20650.020.7082181
GAGTACT28500.020.08233812-13
GTAAGCG600.00476547919.79068694-95
GTGTAGC5050.019.7582361
GGTATCA46100.019.5827161
GTACTTT29500.019.32107714-15
TACATGG21250.019.0003552
ACATGGG20850.018.4523243
GTACGCG650.007566256418.26832614-15
AGTACTT27750.018.05766312-13
GTATAGC3951.8189894E-1118.0432361
GTCTAAC2403.0784267E-617.8176941
Produced by FastQC (version 0.11.5)