##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732972 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799008693374372 31.0 31.0 34.0 30.0 34.0 2 32.05661880672113 33.0 31.0 34.0 30.0 34.0 3 32.104385979273424 33.0 31.0 34.0 30.0 34.0 4 35.7525635358513 37.0 35.0 37.0 33.0 37.0 5 35.64831671605464 37.0 35.0 37.0 33.0 37.0 6 35.68223888497787 37.0 35.0 37.0 33.0 37.0 7 35.60996873004699 37.0 35.0 37.0 33.0 37.0 8 35.59718242988818 37.0 35.0 37.0 33.0 37.0 9 37.133245198998054 39.0 37.0 39.0 33.0 39.0 10-11 37.088079080783444 39.0 37.0 39.0 33.0 39.0 12-13 36.99424671065198 39.0 37.0 39.0 33.0 39.0 14-15 38.01904097291575 40.0 37.0 41.0 33.0 41.0 16-17 38.08661517766026 40.0 37.0 41.0 33.0 41.0 18-19 38.244082993620495 40.0 37.5 41.0 33.0 41.0 20-21 38.233021179526645 40.0 38.0 41.0 33.0 41.0 22-23 38.106276228832755 40.0 37.0 41.0 33.0 41.0 24-25 38.0095897251191 40.0 37.0 41.0 33.0 41.0 26-27 37.809254650928004 40.0 37.0 41.0 32.5 41.0 28-29 37.67969172082972 40.0 37.0 41.0 32.0 41.0 30-31 37.45933268937968 39.0 36.5 41.0 32.0 41.0 32-33 37.316534601594604 39.0 36.0 41.0 32.0 41.0 34-35 37.17451144109188 39.0 36.0 41.0 31.0 41.0 36-37 37.061675616531055 39.0 36.0 41.0 31.0 41.0 38-39 36.86966555339085 39.0 35.5 41.0 31.0 41.0 40-41 36.69835682672735 39.0 35.0 41.0 30.0 41.0 42-43 36.46607646676817 39.0 35.0 40.0 30.0 41.0 44-45 36.17777213863558 39.0 35.0 40.0 30.0 41.0 46-47 35.765751624891536 38.5 35.0 40.0 28.5 41.0 48-49 35.388467090148055 38.0 35.0 40.0 27.0 41.0 50-51 35.0258932128376 38.0 34.0 40.0 26.0 41.0 52-53 34.512803354016256 38.0 33.5 40.0 24.0 41.0 54-55 34.176040148873355 38.0 33.0 40.0 23.5 41.0 56-57 33.827805837057895 38.0 33.0 40.0 23.0 41.0 58-59 33.37621628111306 37.0 32.5 40.0 21.0 41.0 60-61 32.963472001659 37.0 32.0 39.5 19.0 41.0 62-63 32.575785978181976 36.5 31.0 39.0 16.5 40.0 64-65 32.03150802486316 36.0 31.0 39.0 11.0 40.0 66-67 31.446456344853555 35.5 30.0 39.0 8.0 40.0 68-69 30.79799364777918 35.0 29.0 38.0 7.0 40.0 70-71 30.124403114989384 34.0 28.0 38.0 2.0 39.5 72-73 29.60875585970542 34.0 28.0 37.0 2.0 39.0 74-75 28.960358649443634 34.0 26.5 36.5 2.0 39.0 76-77 27.706185229449417 31.5 26.0 35.0 2.0 37.5 78-79 28.25196937945788 33.0 26.0 36.0 2.0 38.0 80-81 27.906855241400763 33.0 26.0 35.0 2.0 37.0 82-83 27.307043788848688 32.5 26.0 35.0 2.0 37.0 84-85 26.602455482610523 32.0 25.0 35.0 2.0 36.0 86-87 25.774172137544134 31.5 22.0 34.0 2.0 35.5 88-89 24.90014898249865 31.0 19.0 34.0 2.0 35.0 90-91 24.144525302467216 31.0 15.0 34.0 2.0 35.0 92-93 23.181076494054345 30.0 4.5 34.0 2.0 35.0 94-95 22.105956298467063 29.0 2.0 34.0 2.0 35.0 96-97 20.79727192853206 29.0 2.0 34.0 2.0 35.0 98-99 19.051121325234796 26.5 2.0 33.0 2.0 34.0 100-101 14.472934163924407 10.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2453779113602259 1101 2 0.22897264716110755 1101 3 0.3069844710057126 1101 4 0.0741291592919282 1101 5 0.12852476081437203 1101 6 0.2715519296217437 1101 7 0.19160739585201014 1101 8 0.12329460204808385 1101 9 0.2690359573114378 1101 10-11 0.21127392411021617 1101 12-13 0.3622408343468706 1101 14-15 0.4981501757516824 1101 16-17 0.4254385651736712 1101 18-19 0.2482663006672965 1101 20-21 0.27627056371414227 1101 22-23 0.20275114599640176 1101 24-25 0.18375527474898945 1101 26-27 0.47505083269761883 1101 28-29 0.2530244915523099 1101 30-31 0.5136138816454192 1101 32-33 0.3597611721201588 1101 34-35 0.37435836208280904 1101 36-37 0.41895803296791456 1101 38-39 0.3784858880958595 1101 40-41 0.45336050827899044 1101 42-43 0.3999027098283392 1101 44-45 0.7014123372742134 1101 46-47 0.853068444148235 1101 48-49 0.7125073936660797 1101 50-51 0.6694384770930384 1101 52-53 0.7031304508375342 1101 54-55 0.5216291055445197 1101 56-57 0.6420794287719715 1101 58-59 0.7898791788667694 1101 60-61 0.8333595797520843 1101 62-63 1.0036976713594683 1101 64-65 1.194759007512591 1101 66-67 1.15563079068518 1101 68-69 1.2844271888614323 1101 70-71 1.4440856962761544 1101 72-73 1.7764864203021773 1101 74-75 1.4187363301108888 1101 76-77 1.5588822111776928 1101 78-79 1.770647765419131 1101 80-81 2.1854902595291215 1101 82-83 2.2277819853113527 1101 84-85 1.938154712528501 1101 86-87 1.8626241707575417 1101 88-89 1.824343912489308 1101 90-91 1.818639841025945 1101 92-93 2.667236220966231 1101 94-95 2.283607631841555 1101 96-97 2.312654839735984 1101 98-99 1.9188953710790528 1101 100-101 2.5178344246199487 1102 1 0.27505055891751695 1102 2 0.2761412419588396 1102 3 0.3258298029635611 1102 4 0.12994671142514846 1102 5 0.22716340203461982 1102 6 0.20993616807137272 1102 7 0.25664037057752154 1102 8 0.22541032675518125 1102 9 0.3376058858354938 1102 10-11 0.28378377968461166 1102 12-13 0.25453644631355843 1102 14-15 0.41761309300170524 1102 16-17 0.46960673627381055 1102 18-19 0.32728820399056247 1102 20-21 0.20794241914115474 1102 22-23 0.3176330633201019 1102 24-25 0.2762318792733396 1102 26-27 0.1920553828413034 1102 28-29 0.10171609497135847 1102 30-31 0.22891903601870922 1102 32-33 0.42526159581775147 1102 34-35 0.22647638949420212 1102 36-37 0.5865972084155615 1102 38-39 0.3483365807493044 1102 40-41 0.18618693963998112 1102 42-43 0.09456872559725582 1102 44-45 0.20862210442475515 1102 46-47 0.09899726295304134 1102 48-49 0.17619374691933842 1102 50-51 0.254659260380933 1102 52-53 -0.052400644881281266 1102 54-55 -0.12742033812212128 1102 56-57 -0.024486686473778718 1102 58-59 -0.011434283663618316 1102 60-61 0.21553480633818367 1102 62-63 0.3026738890294993 1102 64-65 0.1289223889877995 1102 66-67 0.36804835387138723 1102 68-69 0.4100765514728799 1102 70-71 0.5237661730280507 1102 72-73 0.33087629577192956 1102 74-75 0.27059875734543226 1102 76-77 0.31896345058525455 1102 78-79 0.5161367677034114 1102 80-81 0.6276877762928059 1102 82-83 0.4702083908820569 1102 84-85 0.7324195972090735 1102 86-87 0.578396774102913 1102 88-89 0.6239280440934643 1102 90-91 1.3183335813545831 1102 92-93 1.5971488103111575 1102 94-95 1.4139291814694381 1102 96-97 1.198408468797954 1102 98-99 1.8658986317083368 1102 100-101 1.8691948623179826 1103 1 0.2584729222889983 1103 2 0.09398062689950848 1103 3 0.20773213779958866 1103 4 0.1316700120830987 1103 5 0.1984732394599007 1103 6 0.1785901456297907 1103 7 0.26022664089936853 1103 8 0.20514113086187535 1103 9 0.32533864960578285 1103 10-11 0.2907124367563654 1103 12-13 0.22281116880198937 1103 14-15 0.3723012443427436 1103 16-17 0.2802864730944279 1103 18-19 0.23550847705421063 1103 20-21 0.3405280577293439 1103 22-23 0.3901470624520087 1103 24-25 0.40277307303507826 1103 26-27 0.5007453735157128 1103 28-29 0.5319933272043897 1103 30-31 0.2920644679827191 1103 32-33 0.40681669201746473 1103 34-35 0.436070146074762 1103 36-37 0.4023447813979928 1103 38-39 0.5030554592716499 1103 40-41 0.7644467068010101 1103 42-43 0.6037524999265145 1103 44-45 0.5734665561272081 1103 46-47 0.7912688396656975 1103 48-49 0.9362241303000118 1103 50-51 0.8493956727354046 1103 52-53 0.8495044403597873 1103 54-55 0.8791387332723772 1103 56-57 1.102004011570429 1103 58-59 0.8672696309363062 1103 60-61 1.099006805925626 1103 62-63 1.2951500271170175 1103 64-65 1.453663571576012 1103 66-67 1.376152379347019 1103 68-69 1.4544021220744945 1103 70-71 1.1944835989927327 1103 72-73 1.439901921811387 1103 74-75 1.4789183028410022 1103 76-77 1.4914729373338886 1103 78-79 1.506220268281428 1103 80-81 1.3368732696252223 1103 82-83 1.7831056417662694 1103 84-85 1.5853234232803324 1103 86-87 1.7599804487299942 1103 88-89 1.8446759823640626 1103 90-91 2.210287787110669 1103 92-93 2.355903908229873 1103 94-95 2.307866687109918 1103 96-97 2.4549825814700164 1103 98-99 2.251413021913457 1103 100-101 1.9727250501620475 1104 1 0.4960796821122848 1104 2 0.311544138731378 1104 3 0.4356268944257735 1104 4 0.3216782620430365 1104 5 0.24902155514254076 1104 6 0.2927046993900504 1104 7 0.29598042410940906 1104 8 0.33999335370180006 1104 9 0.29494635115111834 1104 10-11 0.4498219159009835 1104 12-13 0.43226419843654895 1104 14-15 0.5672555134497088 1104 16-17 0.5739266838344079 1104 18-19 0.6252221421137492 1104 20-21 0.5642414497686161 1104 22-23 0.6374440629881519 1104 24-25 0.5802154667500758 1104 26-27 0.7895829996639279 1104 28-29 0.691140396094525 1104 30-31 0.6295194839385729 1104 32-33 1.0072485784093814 1104 34-35 0.9207038537887726 1104 36-37 0.9638257778032298 1104 38-39 0.8564861170724143 1104 40-41 0.9608382890721003 1104 42-43 0.820102258659702 1104 44-45 0.8914194185863309 1104 46-47 0.8269227132889725 1104 48-49 1.2470468115567783 1104 50-51 1.2238469119110462 1104 52-53 1.0206594545350782 1104 54-55 1.0344348316546288 1104 56-57 1.261618973871876 1104 58-59 1.1783633540423253 1104 60-61 1.4185473405309494 1104 62-63 1.2946346519219034 1104 64-65 1.6858851057520177 1104 66-67 1.4831891797078782 1104 68-69 1.8013099698241497 1104 70-71 2.0669772037899783 1104 72-73 2.0874238672983445 1104 74-75 1.8363938151002053 1104 76-77 1.8809610639203242 1104 78-79 1.854297354957442 1104 80-81 2.063396989743847 1104 82-83 2.192115705926028 1104 84-85 2.194671003786688 1104 86-87 2.136039144128418 1104 88-89 2.2348575408349127 1104 90-91 2.4731894780556907 1104 92-93 2.7813842161492417 1104 94-95 2.381125144308058 1104 96-97 2.7318112731292494 1104 98-99 3.3324832217524154 1104 100-101 3.1528224669551683 1105 1 0.2934535735684598 1105 2 0.15904739145693725 1105 3 0.18732803250584595 1105 4 0.3092786478835876 1105 5 0.2576225840506723 1105 6 0.17489247512396844 1105 7 0.23115958572874717 1105 8 0.21129945387079374 1105 9 0.36953097435837634 1105 10-11 0.2128720751723776 1105 12-13 0.32831905359679325 1105 14-15 0.3642415080359811 1105 16-17 0.5547084969858886 1105 18-19 0.34940877378557644 1105 20-21 0.3597104404516216 1105 22-23 0.5064162390989395 1105 24-25 0.4750449416779077 1105 26-27 0.4212464136050329 1105 28-29 0.4074550580353957 1105 30-31 0.46989010771412865 1105 32-33 0.4744100706252752 1105 34-35 0.4982355585160647 1105 36-37 0.4991524337022497 1105 38-39 0.4155875796772861 1105 40-41 0.5014197791942792 1105 42-43 0.23692408407433163 1105 44-45 0.4437985817011665 1105 46-47 0.4428599469019687 1105 48-49 0.5587242832543282 1105 50-51 0.5828842546959763 1105 52-53 0.7729737500769787 1105 54-55 0.682689950023935 1105 56-57 0.7884782617119157 1105 58-59 0.6383744581744111 1105 60-61 1.0040585044832682 1105 62-63 0.8516274510604873 1105 64-65 0.47178005393272926 1105 66-67 0.8564814987890372 1105 68-69 1.1537885840882858 1105 70-71 1.3006864699451803 1105 72-73 1.1585510675615538 1105 74-75 1.211992952193242 1105 76-77 1.4312596043064651 1105 78-79 1.5303268239032626 1105 80-81 1.4168205768645485 1105 82-83 1.488936260160557 1105 84-85 1.8395201072308396 1105 86-87 2.0371422667449544 1105 88-89 1.9221425618467691 1105 90-91 1.8715427390065322 1105 92-93 2.082108041055399 1105 94-95 1.7679293601394832 1105 96-97 2.2746762139819836 1105 98-99 2.4754863099723146 1105 100-101 2.524522072141586 1106 1 0.14741407564616793 1106 2 0.15502048831680781 1106 3 0.1890416083101556 1106 4 0.11579987480386222 1106 5 0.1258646694724277 1106 6 0.16318661041077576 1106 7 0.15708491666307367 1106 8 0.01290486020245396 1106 9 -0.15877515595906289 1106 10-11 0.1657112560831422 1106 12-13 0.1475153865445762 1106 14-15 0.1553860695747673 1106 16-17 0.06260433299668478 1106 18-19 0.23892865126490648 1106 20-21 0.30247165366328943 1106 22-23 0.479649649543191 1106 24-25 0.24789708150569822 1106 26-27 0.07625273241580999 1106 28-29 0.3518254260257976 1106 30-31 0.2925216246571054 1106 32-33 0.13677405554580702 1106 34-35 0.08427580612777064 1106 36-37 0.13626259520676598 1106 38-39 0.406261978854765 1106 40-41 0.27999097826895536 1106 42-43 0.13104116414116618 1106 44-45 0.26448626109113604 1106 46-47 -0.13197512142686207 1106 48-49 0.03093490314036984 1106 50-51 0.17604922920653365 1106 52-53 -0.2487672429401968 1106 54-55 -0.3069589811331568 1106 56-57 0.04217315953995637 1106 58-59 0.569196867461983 1106 60-61 0.412044838716227 1106 62-63 -0.1413977599235281 1106 64-65 0.34944412881599973 1106 66-67 0.37324508958713665 1106 68-69 0.5073019499795599 1106 70-71 0.36697240148782484 1106 72-73 0.6108360137981137 1106 74-75 0.5005189557060703 1106 76-77 0.547672983049555 1106 78-79 0.7915266482617405 1106 80-81 0.448055850822481 1106 82-83 0.8556414822828202 1106 84-85 1.2114892200269622 1106 86-87 1.3850061874063933 1106 88-89 1.506945821924738 1106 90-91 1.4206393632622003 1106 92-93 1.5043116995397448 1106 94-95 1.4243707986863718 1106 96-97 1.947179748232081 1106 98-99 1.893301607920307 1106 100-101 1.8393835937968994 1107 1 0.11118442380679028 1107 2 0.07112753778217495 1107 3 0.05893317605977444 1107 4 -0.09851982235252876 1107 5 -0.08671285547931262 1107 6 0.014087132035619732 1107 7 0.029283407921404603 1107 8 0.03181547205059587 1107 9 0.060100201716764445 1107 10-11 0.04056578983815484 1107 12-13 0.14767112099509205 1107 14-15 0.040275207182411066 1107 16-17 -0.07934676296311949 1107 18-19 0.06817609448172846 1107 20-21 0.09757902486576597 1107 22-23 0.129344683838994 1107 24-25 0.16299527977790262 1107 26-27 0.0963655799945684 1107 28-29 -0.0669795944421594 1107 30-31 0.09111419595268444 1107 32-33 0.04690757644502952 1107 34-35 0.0045680050297605135 1107 36-37 0.006641902718989456 1107 38-39 0.12181471635836516 1107 40-41 0.2950125072732135 1107 42-43 0.09626617475164778 1107 44-45 -0.430085965466553 1107 46-47 -0.46446047212031516 1107 48-49 -0.36132338330148883 1107 50-51 -0.32370176680939977 1107 52-53 -0.42925576993189196 1107 54-55 -0.34295160856158446 1107 56-57 -0.2391517127920224 1107 58-59 -0.5430979395642055 1107 60-61 -0.18146000787677963 1107 62-63 0.06999293160588138 1107 64-65 0.13203113474417094 1107 66-67 0.051131482161167696 1107 68-69 0.5094240457887658 1107 70-71 -0.10041235501879342 1107 72-73 0.5543497595025819 1107 74-75 0.47113135148378404 1107 76-77 0.5272786155025635 1107 78-79 0.5306663095478719 1107 80-81 0.87002005585801 1107 82-83 0.7190521009764268 1107 84-85 0.7346328996041862 1107 86-87 1.170393846392443 1107 88-89 1.3327123434674313 1107 90-91 1.3076155136434942 1107 92-93 1.37798710102658 1107 94-95 1.107006236317293 1107 96-97 1.405281191257231 1107 98-99 1.8561674983106329 1107 100-101 1.8065099337977202 1108 1 5.546868038912578E-4 1108 2 0.04547297010885387 1108 3 -0.030518989148689002 1108 4 -0.126728958999216 1108 5 -0.09637374132323373 1108 6 -0.19275657774454658 1108 7 -0.22199062647752044 1108 8 -0.15848691611387977 1108 9 0.0502215599934388 1108 10-11 -0.10793396965534896 1108 12-13 -0.09617483784580116 1108 14-15 0.07670625332065839 1108 16-17 0.03205649492484497 1108 18-19 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4.0 4 8.0 5 25.0 6 56.0 7 163.0 8 373.0 9 609.0 10 1110.0 11 1688.0 12 2335.0 13 3235.0 14 4320.0 15 5757.0 16 7087.0 17 9142.0 18 8767.0 19 7569.0 20 7407.0 21 8024.0 22 8607.0 23 9793.0 24 11085.0 25 12693.0 26 14046.0 27 16613.0 28 19971.0 29 23513.0 30 28013.0 31 33599.0 32 40004.0 33 49198.0 34 60624.0 35 78488.0 36 100130.0 37 110544.0 38 47191.0 39 1179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00131005081906 21.685162036910683 15.581280465504724 22.732247446765538 2 15.251716496927227 22.661579941273427 39.59145189988102 22.495251661918328 3 18.526522593320234 25.418030997598777 32.10161536782362 23.953831041257367 4 12.40620378404632 19.426662955747286 38.339936586936474 29.827196673269917 5 12.504638674539681 38.46852795756431 35.866231540803966 13.16060182709204 6 29.34859721790191 38.44266902419192 18.14489503009665 14.063838727809522 7 25.436442319761195 34.06201055429129 22.430870483456395 18.07067664249112 8 23.686099021788067 36.1087084055282 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19.851372221585546 32-33 20.17682864020945 29.08408876494861 30.58701624010276 20.15206635473918 34-35 20.56236713776194 29.114192620568645 30.400858504957007 19.92258173671241 36-37 20.275539523194926 28.775570902720744 30.77369631713513 20.1751932569492 38-39 19.87535433957244 29.00164079262905 30.78672765912448 20.336277208674026 40-41 20.27688879762203 28.86688175021337 30.653908105338633 20.202321346825972 42-43 20.171624339302916 28.764233809681038 30.42910860127323 20.635033249742815 44-45 20.343030918214488 28.718044220332185 30.2383739491327 20.700550912320626 46-47 20.57374751098441 28.74951993293045 29.856590408481654 20.820142147603484 48-49 20.321308317370924 28.522849440360616 30.218071085935073 20.937771156333394 50-51 20.02327510464247 28.791413587422166 30.194468547229636 20.990842760705732 52-53 20.200225929503446 29.014205181098323 29.794794344122288 20.990774545275944 54-55 20.219871973281382 28.626127601054336 29.705977854542876 21.44802257112141 56-57 20.645399824277053 28.708258978514866 29.255483156246076 21.390858040962 58-59 20.53714193720906 28.792436818868932 29.373222988054117 21.29719825586789 60-61 20.40432649541865 28.957040650938914 29.391641154095925 21.246991699546502 62-63 20.022729381204194 29.004996098077417 29.361899226709888 21.610375294008502 64-65 19.954391132533804 29.146972290191364 29.022888338768972 21.875748238505864 66-67 20.05533673412309 28.9171272376519 28.806753919344253 22.220782108880755 68-69 20.162285503791097 28.60611145787501 28.95940133771279 22.272201700621107 70-71 20.138913995973923 28.49060297705982 28.82144803720199 22.54903498976427 72-73 20.14900988646898 28.049248845282527 29.17801033191604 22.62373093633245 74-75 20.107452944996535 28.537583973194064 28.554910692359325 22.800052389450073 76-77 20.008881648957942 28.149779254869216 28.989920488095045 22.851418608077797 78-79 19.944145206092458 28.14111589528659 28.84523556152213 23.069503337098826 80-81 20.067901685126913 28.061391200067128 28.757052627557822 23.11365448724814 82-83 20.05393174421373 28.006510556119096 28.85109558661516 23.088462113052014 84-85 20.144638740833873 27.88146745355454 28.682635915186932 23.291257890424657 86-87 19.991841150752478 27.922228975784723 28.582781962939997 23.503147910522802 88-89 20.124779664161796 27.790215724475146 28.254353508728848 23.830651102634206 90-91 20.13040168302486 27.482448026238597 28.48003863753571 23.90711165320083 92-93 20.15161633821917 27.505070136029037 28.460909302377935 23.882404223373857 94-95 20.038555360914195 27.475947239457987 28.381507069847146 24.103990329780675 96-97 19.864742445823307 27.558078616918518 28.333688053568213 24.243490883689965 98-99 20.088216193797308 27.397567710635606 27.982105728458933 24.532110367108157 100-101 19.884197407402606 27.141437286442926 28.291959510021876 24.682405796132592 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 609.0 1 360.0 2 135.0 3 146.5 4 109.5 5 104.5 6 168.5 7 324.0 8 461.0 9 502.0 10 444.5 11 354.5 12 343.5 13 376.0 14 441.5 15 534.5 16 586.0 17 646.5 18 767.5 19 914.5 20 1125.5 21 1426.0 22 1812.5 23 2252.5 24 2868.5 25 3638.0 26 4688.5 27 6044.0 28 7611.5 29 9426.0 30 11426.5 31 13564.0 32 15840.0 33 18166.5 34 20554.5 35 22766.5 36 24405.0 37 25690.0 38 26679.5 39 27289.0 40 27882.0 41 28548.5 42 29201.0 43 29891.0 44 30374.5 45 30346.5 46 30090.0 47 31339.0 48 33792.0 49 32913.0 50 29189.0 51 26011.5 52 22913.5 53 19525.5 54 16411.5 55 13809.0 56 11354.5 57 9087.5 58 7197.0 59 5422.5 60 3909.5 61 2792.5 62 1900.0 63 1232.0 64 781.0 65 550.5 66 372.0 67 253.0 68 193.0 69 143.0 70 92.0 71 55.0 72 31.5 73 19.5 74 12.5 75 11.0 76 10.0 77 8.0 78 4.5 79 0.5 80 1.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024011831284141825 2 0.011460192203794961 3 0.0016371703148278514 4 0.0 5 5.457234382759505E-4 6 0.0 7 0.0 8 2.7286171913797525E-4 9 4.0929257870696284E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015689548850433578 26-27 0.009277298450691158 28-29 2.7286171913797525E-4 30-31 0.0 32-33 3.4107714892246904E-4 34-35 0.009550160169829134 36-37 0.0013643085956898762 38-39 0.012892716229269331 40-41 0.01070982247616553 42-43 0.004638649225345579 44-45 8.185851574139257E-4 46-47 2.0464628935348142E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.821542978449381E-5 66-67 6.821542978449381E-4 68-69 6.139388680604443E-4 70-71 6.821542978449381E-5 72-73 6.821542978449381E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.821542978449381E-5 82-83 0.0011596623063363947 84-85 0.005798311531681974 86-87 0.0034789869190091846 88-89 0.0 90-91 8.185851574139257E-4 92-93 4.7750800849145675E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.821542978449381E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 732972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.58069341617455 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78145908779135 51.12162787570785 2 14.443851368340562 19.23363279408553 3 4.662727183574817 9.313428272785739 4 1.8984370200899623 5.055970128181031 5 0.9104731484165077 3.0309966779189352 6 0.47277723664550425 1.8886701748344317 7 0.24664275377591785 1.1495151900732792 8 0.1662054817307768 0.885286097856356 9 0.09895367714944278 0.5929563996621151 >10 0.29391740421565044 3.0509863171729372 >50 0.011459519426661461 0.5289067477554454 >100 0.009412835426056849 1.2368046960620915 >500 0.0022508954213702826 0.9665006058757459 >1k 0.0014323879954174525 1.944718022028531 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3116 0.42511855841696544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.41324907363446356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2030 0.2769546449250449 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1942 0.26494872928297397 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1767 0.24107332885840113 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1377 0.18786529362649595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1013 0.13820446074338447 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 998 0.13615799784984967 RNA PCR Primer, Index 23 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 920 0.12551639080346863 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3643085956898762E-4 0.0 20-21 0.0 0.0 0.0 3.4107714892246904E-4 0.0 22-23 0.0 0.0 0.0 0.0015689548850433576 0.0 24-25 0.0 0.0 0.0 0.004570433795561085 0.0 26-27 0.0 0.0 0.0 0.011187330484656985 0.0 28-29 0.0 0.0 0.0 0.028650480509487403 0.0 30-31 0.0 0.0 0.0 0.07476411104380523 0.0 32-33 0.0 0.0 0.0 0.16494490921890603 0.0 34-35 0.0 0.0 0.0 0.3085383889152655 0.0 36-37 0.0 0.0 0.0 0.5152993565920663 0.0 38-39 0.0 0.0 0.0 0.743616400080767 0.0 40-41 0.0 0.0 0.0 0.9679087332121827 0.0 42-43 0.0 0.0 0.0 1.1946568218158402 0.0 44-45 0.0 0.0 0.0 1.5129500171902883 0.0 46-47 0.0 0.0 0.0 1.8739460716098297 0.0 48-49 0.0 0.0 0.0 2.2046544752050554 0.0 50-51 0.0 0.0 0.0 2.520969423115753 0.0 52-53 0.0 0.0 0.0 2.842264097400719 0.0 54-55 0.0 0.0 0.0 3.2268626905256954 0.0 56-57 0.0 0.0 0.0 3.679335636286243 0.0 58-59 0.0 1.3643085956898762E-4 0.0 4.1417680347953265 0.0 60-61 0.0 1.3643085956898762E-4 0.0 4.5980610446238055 0.0 62-63 0.0 1.3643085956898762E-4 0.0 5.041802415371938 0.0 64-65 0.0 1.3643085956898762E-4 0.0 5.531111693216111 0.0 66-67 0.0 1.3643085956898762E-4 0.0 6.106713489737671 0.0 68-69 0.0 1.3643085956898762E-4 0.0 6.7203112806491925 0.0 70-71 0.0 1.3643085956898762E-4 0.0 7.362491336640417 0.0 72-73 0.0 1.3643085956898762E-4 0.0 7.982774239670819 0.0 74-75 0.0 1.3643085956898762E-4 0.0 8.633958732393598 0.0 76-77 0.0 1.3643085956898762E-4 0.0 9.3483789285266 0.0 78-79 0.0 1.3643085956898762E-4 0.0 10.145476225558411 0.0 80-81 0.0 1.3643085956898762E-4 0.0 10.957785563432164 0.0 82-83 0.0 1.3643085956898762E-4 0.0 11.750517072957766 0.0 84-85 0.0 1.3643085956898762E-4 0.0 12.564258934856992 0.0 86-87 0.0 1.3643085956898762E-4 0.0 13.449013059161878 0.0 88-89 0.0 1.3643085956898762E-4 0.0 14.358461169048748 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTCGC 25 0.0050215367 56.997177 9 GCGGTAT 60 0.003944391 31.675901 1 ATGCCGC 40 4.4955424E-4 29.686031 94-95 GGTTGCG 65 0.005844123 29.229322 7 CTCCGAC 300 0.0 24.540451 94-95 GGAGCGT 105 0.0021960998 22.617928 9 GGGAGCG 110 0.002877209 21.58984 8 GTGTAGA 335 1.8189894E-12 21.27486 1 GTACATG 2065 0.0 20.708218 1 GAGTACT 2850 0.0 20.082338 12-13 GTAAGCG 60 0.004765479 19.790686 94-95 GTGTAGC 505 0.0 19.758236 1 GGTATCA 4610 0.0 19.582716 1 GTACTTT 2950 0.0 19.321077 14-15 TACATGG 2125 0.0 19.000355 2 ACATGGG 2085 0.0 18.452324 3 GTACGCG 65 0.0075662564 18.268326 14-15 AGTACTT 2775 0.0 18.057663 12-13 GTATAGC 395 1.8189894E-11 18.043236 1 GTCTAAC 240 3.0784267E-6 17.817694 1 >>END_MODULE