FastQCFastQC Report
Thu 26 May 2016
SRR938559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586923
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.22853405377740274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48750.1884478200549456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.1230419305097214No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG29330.1133779397376729No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27570.10657449023415078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.041.5153731
GTATCAA67750.038.255361
TATCAAC89200.028.843922
ATCAACG88950.028.7645933
GTACATG95150.028.5385821
TCAACGC90350.028.3715134
CAACGCA91350.028.0088715
TACATGG97800.027.2314682
AACGCAG94750.027.133376
ACATGGG101800.025.7877353
ACGCAGA108550.023.5990777
CGCAGAG110150.023.1263968
CATGGGG63900.022.5508884
GCAGAGT119600.020.7813139
GAGTACT57500.020.53714212-13
GAGTACA83600.019.3409461
AGTACAT74400.019.3043822
GTACTTT60800.019.29999514-15
AGTACTT60700.019.02387812-13
AGAGTAC110750.018.9878710-11
Produced by FastQC (version 0.11.5)