##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938559_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2586923 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.025262444997395 31.0 31.0 34.0 28.0 34.0 2 31.056568363264002 31.0 31.0 34.0 27.0 34.0 3 31.106327865189648 31.0 31.0 34.0 27.0 34.0 4 34.75962369193053 37.0 35.0 37.0 32.0 37.0 5 34.718325980324884 37.0 35.0 37.0 32.0 37.0 6 34.73363142235003 37.0 35.0 37.0 31.0 37.0 7 34.62646704211915 37.0 35.0 37.0 30.0 37.0 8 34.59955437405752 37.0 35.0 37.0 30.0 37.0 9 36.073296344730785 39.0 35.0 39.0 30.0 39.0 10-11 35.89247998490871 38.0 35.0 39.0 30.0 39.0 12-13 35.73571111316417 38.0 35.0 39.0 30.0 39.0 14-15 36.69869976802556 39.0 36.0 41.0 30.0 41.0 16-17 36.52298599533113 39.0 36.0 40.5 30.0 41.0 18-19 36.629293372860346 39.0 36.0 41.0 30.0 41.0 20-21 36.59440462665491 39.0 36.0 41.0 30.0 41.0 22-23 36.497153761437815 39.0 36.0 41.0 29.5 41.0 24-25 36.34441728648282 39.0 35.5 41.0 29.0 41.0 26-27 36.13076326585677 39.0 35.0 40.5 28.0 41.0 28-29 35.91410026506394 39.0 35.0 40.0 27.0 41.0 30-31 35.670003320547224 38.0 34.5 40.0 27.0 41.0 32-33 35.42380484459723 38.0 34.0 40.0 26.5 41.0 34-35 35.16174563371233 38.0 34.0 40.0 25.5 41.0 36-37 34.88399693380901 38.0 34.0 40.0 25.0 41.0 38-39 34.78457128410857 38.0 33.5 40.0 24.5 41.0 40-41 34.68463208993851 38.0 33.0 40.0 24.0 41.0 42-43 34.40217161469437 38.0 33.0 40.0 24.0 41.0 44-45 34.05905742072726 38.0 33.0 40.0 23.0 41.0 46-47 33.673398087225635 38.0 32.5 40.0 21.5 41.0 48-49 33.321383937596906 37.0 32.0 40.0 19.5 41.0 50-51 32.610919807044894 36.5 30.5 39.5 18.0 40.5 52-53 32.62973037852306 37.0 31.0 39.0 18.0 40.0 54-55 32.71853027708981 37.0 31.0 40.0 16.5 41.0 56-57 32.38182079636696 36.0 31.0 39.5 13.5 41.0 58-59 31.989423148659625 36.0 31.0 39.0 10.0 41.0 60-61 31.494306943036186 35.5 30.0 39.0 8.0 40.0 62-63 30.908200785257236 35.0 29.0 38.5 6.5 40.0 64-65 30.374771108378564 35.0 28.5 38.0 2.0 40.0 66-67 29.823931172284603 34.0 28.0 37.5 2.0 40.0 68-69 29.24825845222297 34.0 27.5 37.0 2.0 39.0 70-71 28.60921701187086 34.0 26.0 36.0 2.0 39.0 72-73 27.970584551608223 33.0 26.0 36.0 2.0 39.0 74-75 27.291420540928353 33.0 25.0 35.0 2.0 38.0 76-77 26.5534778190151 32.0 23.0 35.0 2.0 37.0 78-79 25.77521692760086 31.5 20.5 35.0 2.0 36.5 80-81 25.0874886882988 31.0 18.5 34.5 2.0 36.0 82-83 24.378960834937878 31.0 15.0 34.0 2.0 35.5 84-85 23.67815451020382 30.0 7.5 34.0 2.0 35.0 86-87 22.919750800468357 30.0 3.5 34.0 2.0 35.0 88-89 22.112568097310977 29.0 2.0 34.0 2.0 35.0 90-91 21.219388439470364 29.0 2.0 34.0 2.0 35.0 92-93 20.273828791966363 27.0 2.0 33.5 2.0 35.0 94-95 19.27202974344424 25.5 2.0 33.0 2.0 35.0 96-97 18.0207675296095 24.0 2.0 32.5 2.0 34.0 98-99 16.520506022019212 10.5 2.0 32.0 2.0 34.0 100-101 13.139003557508282 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5732291450090798 1101 2 0.37836099098769793 1101 3 0.46072455733611406 1101 4 0.28740641577179815 1101 5 0.296748330202405 1101 6 0.5147668870828142 1101 7 0.40923346652960646 1101 8 0.4478495977018966 1101 9 0.6289084873352664 1101 10-11 0.6291877131974886 1101 12-13 0.6906480918428173 1101 14-15 0.7523047733097386 1101 16-17 0.4713621540423887 1101 18-19 0.6213849666110178 1101 20-21 0.6426294064168516 1101 22-23 0.6971024515838806 1101 24-25 0.6746087743597897 1101 26-27 0.7661245673966164 1101 28-29 0.6916755792423785 1101 30-31 0.8111740156600575 1101 32-33 0.7504001127268793 1101 34-35 0.6287918180947614 1101 36-37 0.7760030075688746 1101 38-39 0.7606297338616486 1101 40-41 0.8243051644332624 1101 42-43 1.025044460026578 1101 44-45 1.0987590185403207 1101 46-47 1.01754384170615 1101 48-49 0.7512024046375032 1101 50-51 0.8690567019507469 1101 52-53 0.8646559337069917 1101 54-55 0.8238380356083042 1101 56-57 1.0289224302317663 1101 58-59 0.8994304659728272 1101 60-61 1.1025039153405807 1101 62-63 0.9053990579027662 1101 64-65 0.8251700067706906 1101 66-67 0.9246782018697566 1101 68-69 1.0554715928591456 1101 70-71 1.0470463689583553 1101 72-73 1.1491570638454007 1101 74-75 0.9306337910499778 1101 76-77 0.8508985897931858 1101 78-79 1.1050898083683478 1101 80-81 1.118080332311088 1101 82-83 1.1205529626698087 1101 84-85 1.2198102138678308 1101 86-87 1.1635778421525984 1101 88-89 1.2358384943826266 1101 90-91 0.9489633903461119 1101 92-93 0.7705155334008076 1101 94-95 0.6878416558961931 1101 96-97 0.6080362488922404 1101 98-99 0.6982582586241612 1101 100-101 0.785559367611862 1102 1 0.38141590733261665 1102 2 0.2756879986248215 1102 3 0.16200030369112284 1102 4 0.16640144007173063 1102 5 0.25252245656204764 1102 6 0.4351180789365472 1102 7 0.2959986829146999 1102 8 0.34445050027074586 1102 9 0.49883443040400266 1102 10-11 0.695160578275015 1102 12-13 0.6305375850156949 1102 14-15 0.7295351597943522 1102 16-17 0.7122800549912469 1102 18-19 0.7593796437690798 1102 20-21 0.7577445418021895 1102 22-23 0.7049362863443491 1102 24-25 0.6902749974562923 1102 26-27 0.6231874579312162 1102 28-29 0.596817618122266 1102 30-31 0.6677234255188864 1102 32-33 0.6250225959483657 1102 34-35 0.6105668701660463 1102 36-37 0.7928596379785091 1102 38-39 0.6900384355510667 1102 40-41 0.7600347409201831 1102 42-43 1.0456256333660718 1102 44-45 0.9170201270800149 1102 46-47 0.7646640106485236 1102 48-49 0.8147539552045018 1102 50-51 0.7399474101201449 1102 52-53 0.5733417124619038 1102 54-55 0.7243544091329568 1102 56-57 0.7213851124812507 1102 58-59 0.7725602391734796 1102 60-61 0.7519832025426219 1102 62-63 0.6625111268682851 1102 64-65 0.8358431727045001 1102 66-67 0.6392292317239558 1102 68-69 0.7783352239630581 1102 70-71 0.8109956862460201 1102 72-73 0.7291428888858995 1102 74-75 0.6611562395572683 1102 76-77 0.7171911146716816 1102 78-79 0.9324547123257716 1102 80-81 1.0620067960926178 1102 82-83 1.0095746011794127 1102 84-85 1.0474832062307087 1102 86-87 1.13489958480476 1102 88-89 0.8894487697818398 1102 90-91 1.0185769057360687 1102 92-93 1.0712459777423966 1102 94-95 1.005839515188022 1102 96-97 1.392135705036651 1102 98-99 1.4981815981289053 1102 100-101 1.3643837138280155 1103 1 0.33688702195296116 1103 2 0.14161882285607774 1103 3 0.1991570567726697 1103 4 0.031707182055328076 1103 5 0.19998813187019948 1103 6 0.31469927383061247 1103 7 0.2229983977894605 1103 8 0.31100037525429514 1103 9 0.5001531346194668 1103 10-11 0.5497590451785541 1103 12-13 0.5980799151468545 1103 14-15 0.6444348724758484 1103 16-17 0.6207480003349346 1103 18-19 0.6293337495724742 1103 20-21 0.5075677093242277 1103 22-23 0.5947134500062319 1103 24-25 0.7222789343778189 1103 26-27 0.7969562103986405 1103 28-29 0.5757512497404704 1103 30-31 0.6496248270190819 1103 32-33 0.6910455375634825 1103 34-35 0.7975432267041853 1103 36-37 0.7835080785627895 1103 38-39 0.5561447214658841 1103 40-41 0.7467147317084439 1103 42-43 0.9129656342433918 1103 44-45 0.7942120273300475 1103 46-47 0.35629750409064087 1103 48-49 0.44728647044772885 1103 50-51 0.684786643352588 1103 52-53 0.750690979909372 1103 54-55 0.7791738480942172 1103 56-57 0.6997402688614685 1103 58-59 0.7144152529910812 1103 60-61 0.7878459257079449 1103 62-63 0.7799539373258 1103 64-65 1.090035061988182 1103 66-67 1.0196452404093073 1103 68-69 1.0677729789308152 1103 70-71 0.9688234706036134 1103 72-73 0.9569072328785317 1103 74-75 1.2027461417373786 1103 76-77 1.0800582134127445 1103 78-79 0.9667629546383552 1103 80-81 1.0889305689574726 1103 82-83 1.2569317095708676 1103 84-85 1.212802381999449 1103 86-87 1.2964463343770767 1103 88-89 1.1843326909118126 1103 90-91 1.1139622634585749 1103 92-93 1.2426898629027257 1103 94-95 1.094995983144333 1103 96-97 1.1738394379839683 1103 98-99 1.1239259394896095 1103 100-101 1.3622891399805788 1104 1 0.47346211821034956 1104 2 0.4013325232974019 1104 3 0.4996390861444091 1104 4 0.23112056719075724 1104 5 0.4331650388769148 1104 6 0.4394368417143468 1104 7 0.4441084307661711 1104 8 0.6471580010861402 1104 9 0.6016565833583698 1104 10-11 0.729811444447229 1104 12-13 0.6595090436268976 1104 14-15 0.7566344474197493 1104 16-17 0.9134798089150777 1104 18-19 0.7313750550318332 1104 20-21 0.7996367396230326 1104 22-23 0.802227691709767 1104 24-25 0.7707786813067514 1104 26-27 0.8061945955986332 1104 28-29 0.7812873246340075 1104 30-31 0.8902121326643808 1104 32-33 0.8725331629046806 1104 34-35 0.8827947677262316 1104 36-37 1.0748367791461533 1104 38-39 1.1529084944714683 1104 40-41 1.0628190576743108 1104 42-43 1.1951188139006064 1104 44-45 1.1371669839076972 1104 46-47 0.9434369907953766 1104 48-49 0.9712516389594938 1104 50-51 0.96535280271506 1104 52-53 1.084026088613733 1104 54-55 1.004953752443683 1104 56-57 1.1120037733018364 1104 58-59 0.9936294673216182 1104 60-61 0.9013215082233224 1104 62-63 1.0563154532215044 1104 64-65 1.2487819599308168 1104 66-67 1.1682474262461078 1104 68-69 1.3539038773589347 1104 70-71 1.449844431602262 1104 72-73 1.4677458729572415 1104 74-75 1.1414282160421436 1104 76-77 1.2229596022047957 1104 78-79 1.3995503936624303 1104 80-81 1.319249370717607 1104 82-83 1.3422922745132944 1104 84-85 1.4037134346201547 1104 86-87 1.517098240931876 1104 88-89 1.705203710529986 1104 90-91 1.7021073364489219 1104 92-93 1.5516984343940088 1104 94-95 1.6054634899811226 1104 96-97 1.8092804756528231 1104 98-99 1.8585341990320678 1104 100-101 1.5998282949814442 1105 1 0.4804477147125539 1105 2 0.30233288150158444 1105 3 0.4571639106166714 1105 4 0.44399101257845786 1105 5 0.44281702737133344 1105 6 0.5677053011442652 1105 7 0.6160310648121765 1105 8 0.5090615257845528 1105 9 0.7156537576961952 1105 10-11 0.721921343950207 1105 12-13 0.7805078798476472 1105 14-15 0.7432052496279979 1105 16-17 0.840975900184894 1105 18-19 0.7525184028112051 1105 20-21 0.7291239388199173 1105 22-23 0.7931929520534808 1105 24-25 0.9306768006579205 1105 26-27 0.8598653429232499 1105 28-29 0.7557420625376992 1105 30-31 0.9287047079987119 1105 32-33 1.1006189843222103 1105 34-35 1.2142121186448733 1105 36-37 1.1313565564753603 1105 38-39 1.0282342219872334 1105 40-41 1.0755991064700225 1105 42-43 1.1756365034989997 1105 44-45 1.0849617221496075 1105 46-47 1.1178516036811956 1105 48-49 1.0606122060597443 1105 50-51 0.9912105326229508 1105 52-53 0.9372709880475227 1105 54-55 1.0538645350045215 1105 56-57 0.9205248051143613 1105 58-59 1.0778737103039688 1105 60-61 1.189333044550299 1105 62-63 1.3504731891250934 1105 64-65 1.2326007393615939 1105 66-67 1.3482945308647913 1105 68-69 1.1828288650985108 1105 70-71 1.1208656363674514 1105 72-73 1.0716312353199733 1105 74-75 1.2982978189582042 1105 76-77 1.3828680987157647 1105 78-79 1.5151243582140523 1105 80-81 1.4473797754883293 1105 82-83 1.4727106508490664 1105 84-85 1.5863052494239795 1105 86-87 1.7355060925215788 1105 88-89 1.5723154164274433 1105 90-91 1.466337266637538 1105 92-93 1.6190706454362314 1105 94-95 1.5887641032328368 1105 96-97 1.7668414280147395 1105 98-99 1.7173688516739354 1105 100-101 1.331980271945687 1106 1 0.161830788567368 1106 2 0.12848302585670623 1106 3 0.08753709979026425 1106 4 0.245842305011422 1106 5 0.2078996201426051 1106 6 0.04742028807640253 1106 7 0.43534143658860813 1106 8 0.2791646108210628 1106 9 0.06121842593491067 1106 10-11 -0.025634162510080216 1106 12-13 0.05624757773139066 1106 14-15 0.3144603952380578 1106 16-17 0.14840436512110244 1106 18-19 0.05435519210081452 1106 20-21 0.15532661010354332 1106 22-23 0.09648328694834873 1106 24-25 0.06335193174980702 1106 26-27 0.019742659856753164 1106 28-29 0.1771457714348159 1106 30-31 -0.1295142909687783 1106 32-33 0.10879379754727836 1106 34-35 -0.30718482712839545 1106 36-37 -0.2541058082433807 1106 38-39 0.0020241112673815564 1106 40-41 0.20752886616872246 1106 42-43 -0.3282644663329535 1106 44-45 -0.01111040581471201 1106 46-47 0.09684977463192723 1106 48-49 0.47777911845324894 1106 50-51 0.057759070059852036 1106 52-53 0.011942863822461902 1106 54-55 0.32811724739291037 1106 56-57 -0.5828180567090016 1106 58-59 -0.18210963738221508 1106 60-61 0.47229237713517946 1106 62-63 0.14041041230667872 1106 64-65 0.008995558508146928 1106 66-67 -0.18069116587973966 1106 68-69 -0.14123771109258598 1106 70-71 -0.28708576833336963 1106 72-73 0.14878946085663003 1106 74-75 0.2796954711021904 1106 76-77 0.3237158638039155 1106 78-79 0.5835582291405856 1106 80-81 0.6764025942681755 1106 82-83 0.5448421470173805 1106 84-85 0.49900451735339857 1106 86-87 0.19746066537019047 1106 88-89 0.3484643584494336 1106 90-91 0.38638414260034537 1106 92-93 0.39957556523309634 1106 94-95 0.4381509884836632 1106 96-97 0.3778698286261246 1106 98-99 0.6521867658363725 1106 100-101 0.313935055202597 1107 1 0.24714246357915215 1107 2 0.00989294290165077 1107 3 0.17369909071162581 1107 4 0.09291258470567954 1107 5 0.1033253627870323 1107 6 0.26675360014829863 1107 7 0.16347974935149523 1107 8 0.16448436518054166 1107 9 0.41764069233368417 1107 10-11 0.41173714375744197 1107 12-13 0.3300580886792659 1107 14-15 0.4685231377513759 1107 16-17 0.6119272442037342 1107 18-19 0.5273632962535331 1107 20-21 0.43333448392397855 1107 22-23 0.4430412367784342 1107 24-25 0.4905293691082875 1107 26-27 0.3952292810948137 1107 28-29 0.6623707706375086 1107 30-31 0.6029491659289548 1107 32-33 0.7241730485066995 1107 34-35 0.5067473701352512 1107 36-37 0.5897897839054878 1107 38-39 0.7671818534440575 1107 40-41 0.6310747784794515 1107 42-43 0.8045802082075255 1107 44-45 0.7213448299923968 1107 46-47 0.7281571760940082 1107 48-49 0.47312782380864604 1107 50-51 0.4065962463986992 1107 52-53 0.782533187740519 1107 54-55 0.898620541144524 1107 56-57 0.942466403657896 1107 58-59 0.6516251828345787 1107 60-61 0.7082449782953439 1107 62-63 0.8162840626481014 1107 64-65 0.8620716992730628 1107 66-67 0.9027935134800025 1107 68-69 0.9841171955165287 1107 70-71 1.1781836672816723 1107 72-73 1.1620264089893446 1107 74-75 1.1484952273043305 1107 76-77 1.0942056919760361 1107 78-79 1.0027954394977385 1107 80-81 1.1849116356960891 1107 82-83 1.0568440888956872 1107 84-85 1.2398378949813988 1107 86-87 1.2138075953952416 1107 88-89 1.136124005296896 1107 90-91 1.1101172973375704 1107 92-93 1.2574235530148563 1107 94-95 1.5670491851590818 1107 96-97 1.6612140407302647 1107 98-99 1.680531668937352 1107 100-101 1.5807112182920253 1108 1 0.3115905735073099 1108 2 0.2772318471643658 1108 3 0.3195713319428677 1108 4 0.23346754196947472 1108 5 0.2557609858359555 1108 6 0.28849235718579536 1108 7 0.35345453021766815 1108 8 0.3671052466233533 1108 9 0.3997345620393702 1108 10-11 0.4956781446162495 1108 12-13 0.38807981803024205 1108 14-15 0.48328321518093276 1108 16-17 0.6086645698849935 1108 18-19 0.5426580874799996 1108 20-21 0.4300413421651754 1108 22-23 0.26974489150804715 1108 24-25 0.5237889762350818 1108 26-27 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-1.7967034884140816 2316 76-77 -2.128893980239166 2316 78-79 -2.1326252786988924 2316 80-81 -2.4821931150470675 2316 82-83 -2.501466934317822 2316 84-85 -2.61002867157967 2316 86-87 -2.6973883002751897 2316 88-89 -3.0472519339560975 2316 90-91 -2.677951458117878 2316 92-93 -2.875552818401335 2316 94-95 -3.1769270824891045 2316 96-97 -3.186842384645283 2316 98-99 -3.2184664815506636 2316 100-101 -2.884604327985448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10304.0 3 3877.0 4 4289.0 5 4766.0 6 5605.0 7 6927.0 8 8529.0 9 10644.0 10 12759.0 11 15417.0 12 18195.0 13 20749.0 14 22832.0 15 25640.0 16 29433.0 17 33286.0 18 33469.0 19 32920.0 20 35627.0 21 38976.0 22 42974.0 23 47546.0 24 52826.0 25 59061.0 26 65960.0 27 74019.0 28 83756.0 29 95609.0 30 109802.0 31 127230.0 32 148939.0 33 175777.0 34 211564.0 35 255757.0 36 290553.0 37 263975.0 38 103541.0 39 3790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59102392370393 17.379042935867773 13.420159929487216 24.60977321094108 2 16.19387858557512 19.04105528497694 38.97918638605605 25.78587974339189 3 19.67104435676594 22.60320015789871 28.57184875006531 29.15390673527004 4 13.63177047877168 15.248407145369972 34.42957750021963 36.69024487563872 5 15.20375957083034 36.095349010084995 32.80556966573333 15.895321753351338 6 34.8841717516048 33.588179985528726 16.47009205124534 15.057556211621131 7 31.056011816382085 31.165166419203167 19.102906149467955 18.675915614946796 8 27.800034877547713 34.31177356970154 18.64305459264223 19.245136960108514 9 27.408747510452137 16.268680550265955 18.81976388782023 37.50280805146168 10-11 26.610190599475008 25.27818720681132 26.039950819526304 22.071671374187368 12-13 26.871749094334447 22.443449204505306 26.718356916832704 23.966444784327543 14-15 23.54201825185064 24.133947695917975 24.46168679301532 27.86234725921607 16-17 24.086081212134335 27.407645287373033 24.953250356905876 23.553023143586753 18-19 24.051138069733344 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24.104209210356885 44-45 23.784366806065236 25.49279344494884 26.638786952138965 24.084052796846958 46-47 23.944899683169314 25.78218905068711 26.16228922253731 24.110622043606263 48-49 24.20399993427837 25.549920892422396 26.177659092249005 24.06842008105023 50-51 23.99120570523494 25.768582625507552 26.187382498858575 24.052829170398933 52-53 24.279936813977006 25.70966362643854 25.998029109488417 24.012370450096032 54-55 23.881640562705083 25.547545293313167 26.2574312928589 24.31338285112285 56-57 23.87866132566076 25.95236705252483 26.27123067245718 23.897740949357228 58-59 24.108147942227838 25.365531994935818 26.191254528734227 24.33506553410211 60-61 23.863936903384662 25.49273931118961 26.647478053216695 23.99584573220903 62-63 23.769122519436635 25.718739972396303 26.330718585329834 24.181418922837228 64-65 23.601575848180122 25.519350067973484 26.42822114374444 24.450852940101957 66-67 23.733364003811904 25.631989051412358 26.33883404500442 24.295812899771324 68-69 23.630158633507595 25.392804709616495 26.53326877037527 24.443767886500638 70-71 23.604598241612237 25.64967041346512 26.355245060262117 24.39048628466053 72-73 23.89430387394572 25.510408661398294 26.13638802291017 24.458899441745814 74-75 23.599819886042543 25.832838548408333 26.125011382792234 24.44233018275689 76-77 23.8669600323705 25.59943131234883 26.112170429982072 24.421438225298598 78-79 23.925815793166556 25.601839663797847 26.12262286234956 24.34972168068604 80-81 23.713846006350746 26.002771010467836 26.12050416308757 24.162878820093848 82-83 23.920439422543698 25.918944501390445 25.843908505567875 24.316707570497982 84-85 23.863751898595204 25.494253282361363 25.97666752616476 24.66532729287868 86-87 23.974602541292327 25.44553418094626 26.118784113868703 24.46107916389271 88-89 23.930914803625377 25.483175830815707 25.986919637462236 24.598989728096676 90-91 23.80025966668697 25.644395849586672 26.035481335610978 24.519863148115387 92-93 23.987851472281072 25.233657508032188 26.064562205620156 24.713928814066584 94-95 23.83049250023543 25.207945424755906 26.154685677788482 24.806876397220186 96-97 23.96703467542834 25.180311144524264 26.032655943519394 24.819998236528 98-99 24.05361315365874 25.20074579571585 25.748009361886083 24.99763168873933 100-101 24.715386516889577 24.535173541787476 25.893356936197602 24.856083005125345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 682.0 1 460.5 2 370.0 3 508.5 4 445.0 5 440.0 6 734.5 7 1383.0 8 2123.0 9 2416.0 10 2066.0 11 1705.5 12 1666.0 13 1743.0 14 1892.5 15 2036.5 16 2135.5 17 2210.5 18 2300.5 19 2471.5 20 2737.5 21 3093.5 22 3675.0 23 4394.5 24 5176.5 25 6292.0 26 7694.0 27 9263.5 28 10977.5 29 12970.5 30 15507.5 31 18307.0 32 21969.5 33 25318.0 34 27428.5 35 30070.0 36 34380.5 37 39501.5 38 44805.5 39 49184.0 40 54106.5 41 63316.0 42 72255.0 43 79567.0 44 86685.5 45 93207.0 46 98298.0 47 102472.5 48 108303.0 49 109827.0 50 108804.5 51 107980.0 52 107015.0 53 108236.0 54 109099.5 55 105148.5 56 96831.5 57 89472.5 58 82301.5 59 73260.5 60 62880.5 61 53057.0 62 44605.5 63 35984.0 64 28871.5 65 23382.0 66 18287.5 67 14986.5 68 11569.0 69 8034.5 70 6175.0 71 4879.0 72 3979.5 73 3255.5 74 2499.0 75 2076.5 76 1507.0 77 925.0 78 586.5 79 403.0 80 234.0 81 131.5 82 78.0 83 43.5 84 26.0 85 20.5 86 16.0 87 11.0 88 8.5 89 7.5 90 6.0 91 5.0 92 3.5 93 1.0 94 0.5 95 2.0 96 2.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006107642167934647 2 0.1062652425294452 3 0.11550401770752357 4 0.11751412778810966 5 0.1575230495843904 6 0.09636931597886757 7 0.026402022789236475 8 0.02829616498055798 9 0.02358013748379832 10-11 0.05195361439053269 12-13 0.053867084563398296 14-15 0.02781296544195556 16-17 0.03119536221217253 18-19 0.03647190117371101 20-21 0.0589696716910399 22-23 0.03144662597224579 24-25 0.047198930930684835 26-27 0.05746208913060032 28-29 0.053693132729501415 30-31 0.06807315099830957 32-33 0.04957627266060877 34-35 0.06237139644280097 36-37 0.05309396530163441 38-39 0.032335713123274254 40-41 0.037341660343195374 42-43 0.033089504403494036 44-45 0.0083303600455058 46-47 0.01422539441645538 48-49 0.009876598569033558 50-51 0.009509366919695715 52-53 0.01063038984925334 54-55 0.012002676538884226 56-57 0.015230449456748424 58-59 0.02015908475049315 60-61 0.025783527379825377 62-63 0.012099316446604712 64-65 0.009760630679768977 66-67 0.011132917369399862 68-69 0.011616116908002287 70-71 0.03107939432290795 72-73 0.028431460851366663 74-75 0.030248291116511776 76-77 0.023870057206959776 78-79 0.005643770610876319 80-81 0.005450490795435349 82-83 0.006358905928007907 84-85 0.005489146758523543 86-87 0.0141094265271908 88-89 0.038095451623415155 90-91 0.03538953420724158 92-93 0.02961046772555658 94-95 0.046715731392082405 96-97 0.043101398843336275 98-99 0.026749926457030223 100-101 0.07445138490786159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2586923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.15789913758767 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91663392778237 35.59669801284134 2 13.366398448851752 12.873953366652218 3 4.994160737212562 7.215248671787491 4 2.380823276719289 4.58621788898674 5 1.3497495759012834 3.2500551968627867 6 0.8743998929199647 2.526555710949427 7 0.581313619335486 1.9596389853085057 8 0.425035481418656 1.6375052675244521 9 0.30785466801646655 1.334307064623528 >10 1.5903486556042061 13.729971660785717 >50 0.12215816082528588 4.111708304324459 >100 0.08427148789467973 7.859266839562318 >500 0.005159203778309869 1.6752562007068421 >1k 0.0016122511807203411 1.4141052155563727 >5k 8.061255903601707E-5 0.22951161352795466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5912 0.22853405377740274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4875 0.1884478200549456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3183 0.1230419305097214 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2933 0.1133779397376729 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2757 0.10657449023415078 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.5462385235277587E-4 0.0 22-23 0.0 0.0 0.0 4.2521559397013366E-4 0.0 24-25 0.0 0.0 0.0 9.277431141166551E-4 0.0 26-27 0.0 0.0 0.0 0.0014882545788954676 0.0 28-29 0.0 0.0 0.0 0.003923580253451688 0.0 30-31 0.0 0.0 0.0 0.012157300391237 0.0 32-33 0.0 0.0 0.0 0.028586084703719438 0.0 34-35 0.0 0.0 0.0 0.05498810749295592 0.0 36-37 0.0 0.0 0.0 0.09820547422555677 0.0 38-39 0.0 0.0 0.0 0.16537021009129377 0.0 40-41 0.0 0.0 0.0 0.2357433908933509 1.9327981544096984E-5 42-43 0.0 0.0 0.0 0.310098135893492 3.865596308819397E-5 44-45 0.0 0.0 0.0 0.40022451383361624 3.865596308819397E-5 46-47 0.0 0.0 0.0 0.5096982012993815 3.865596308819397E-5 48-49 0.0 0.0 0.0 0.6291257992603567 3.865596308819397E-5 50-51 0.0 0.0 0.0 0.7578888123071309 3.865596308819397E-5 52-53 0.0 0.0 0.0 0.881665206115528 7.731192617638794E-5 54-55 0.0 0.0 0.0 1.023571246612288 7.731192617638794E-5 56-57 0.0 0.0 0.0 1.1872985782723335 7.731192617638794E-5 58-59 0.0 0.0 0.0 1.3663336713153038 7.731192617638794E-5 60-61 0.0 0.0 0.0 1.5628026037110496 7.731192617638794E-5 62-63 0.0 0.0 0.0 1.7580732012510616 7.731192617638794E-5 64-65 0.0 0.0 0.0 1.9632783813047392 7.731192617638794E-5 66-67 0.0 0.0 0.0 2.1994083318289723 7.731192617638794E-5 68-69 0.0 0.0 0.0 2.4633512477951607 7.731192617638794E-5 70-71 0.0 0.0 0.0 2.7481297278658854 7.731192617638794E-5 72-73 0.0 0.0 0.0 3.0305115382251424 7.731192617638794E-5 74-75 0.0 0.0 0.0 3.3192522545124072 7.731192617638794E-5 76-77 0.0 0.0 0.0 3.6387631174178745 7.731192617638794E-5 78-79 0.0 0.0 0.0 3.9899911980372047 7.731192617638794E-5 80-81 0.0 3.865596308819397E-5 0.0 4.365263287697392 7.731192617638794E-5 82-83 0.0 3.865596308819397E-5 0.0 4.752190150228669 9.663990772048491E-5 84-85 0.0 3.865596308819397E-5 0.0 5.141513682471415 1.159678892645819E-4 86-87 0.0 3.865596308819397E-5 0.0 5.546744143524952 1.159678892645819E-4 88-89 0.0 3.865596308819397E-5 0.0 5.979516978278828 1.159678892645819E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 41.515373 1 GTATCAA 6775 0.0 38.25536 1 TATCAAC 8920 0.0 28.84392 2 ATCAACG 8895 0.0 28.764593 3 GTACATG 9515 0.0 28.538582 1 TCAACGC 9035 0.0 28.371513 4 CAACGCA 9135 0.0 28.008871 5 TACATGG 9780 0.0 27.231468 2 AACGCAG 9475 0.0 27.13337 6 ACATGGG 10180 0.0 25.787735 3 ACGCAGA 10855 0.0 23.599077 7 CGCAGAG 11015 0.0 23.126396 8 CATGGGG 6390 0.0 22.550888 4 GCAGAGT 11960 0.0 20.781313 9 GAGTACT 5750 0.0 20.537142 12-13 GAGTACA 8360 0.0 19.340946 1 AGTACAT 7440 0.0 19.304382 2 GTACTTT 6080 0.0 19.299995 14-15 AGTACTT 6070 0.0 19.023878 12-13 AGAGTAC 11075 0.0 18.98787 10-11 >>END_MODULE