FastQCFastQC Report
Thu 26 May 2016
SRR938559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586923
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58980.227992870294168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.19586976496787883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.13653286162750108No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG30290.11708891219413951No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC26730.10332738933474248No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG26460.10228367833136123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49900.039.0487371
GTATCAA74450.035.6199381
GTACATG93150.028.5712221
ATCAACG94250.027.9217153
TCAACGC95100.027.62224
TATCAAC97250.027.1712022
TACATGG96650.027.143192
CAACGCA97250.027.011535
AACGCAG101050.025.9852096
ACATGGG100000.025.4136753
CATGGGG66200.023.8946084
ACGCAGA117800.022.250067
CGCAGAG119250.021.9396958
GAGTACT60150.021.07720412-13
TAAGGTG9600.020.782215
GTACTTT61500.020.34430314-15
GTAAGGT7050.020.21374
GCAGAGT130450.019.6920349
ATGGGGA39850.019.1916035
AGTACTT61550.019.09345412-13
Produced by FastQC (version 0.11.5)