FastQCFastQC Report
Thu 26 May 2016
SRR938558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560117
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61300.23944218174403747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.18608524532277237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.13007218029488496No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG28530.11144021933372576No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27300.1066357514129237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72950.035.2393571
GGTATCA49650.032.6960031
GTACATG93350.030.6492371
TACATGG95950.029.6217252
ATCAACG92250.027.6673
ACATGGG99750.027.254443
TATCAAC93650.027.2533992
TCAACGC93550.027.180924
CAACGCA95250.026.5928845
AACGCAG98800.025.730056
CATGGGG63700.023.577724
ACGCAGA110300.023.0020797
CGCAGAG113900.022.149928
GAGTACT53700.021.5831612-13
GCAGAGT121100.020.4778949
AGTACTT57700.020.3750612-13
GTACTTT56950.020.01861414-15
CATGGGA46500.019.931314
ATGGGGA38150.019.3081385
GTGTAGG12950.019.1158281
Produced by FastQC (version 0.11.5)