##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938558_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2560117 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.971796992090596 31.0 31.0 34.0 28.0 34.0 2 31.061958887035242 31.0 31.0 34.0 27.0 34.0 3 31.133157586157196 31.0 31.0 34.0 27.0 34.0 4 34.75796848347166 37.0 35.0 37.0 32.0 37.0 5 34.766917683840234 37.0 35.0 37.0 32.0 37.0 6 34.79913066473134 37.0 35.0 37.0 32.0 37.0 7 34.70410922625802 37.0 35.0 37.0 30.0 37.0 8 34.66376692940205 37.0 35.0 37.0 30.0 37.0 9 36.15240944066228 39.0 35.0 39.0 31.0 39.0 10-11 35.99640700014882 39.0 35.0 39.0 30.0 39.0 12-13 35.825269509166965 38.0 35.0 39.0 30.0 39.0 14-15 36.80768652370185 39.0 36.0 41.0 30.5 41.0 16-17 36.66260467783309 39.0 36.0 41.0 30.0 41.0 18-19 36.61767548123777 39.0 36.0 40.5 30.0 41.0 20-21 36.6851823569001 39.0 36.0 41.0 30.0 41.0 22-23 36.593572481257695 39.0 36.0 41.0 30.0 41.0 24-25 36.464154177328616 39.0 36.0 41.0 29.5 41.0 26-27 36.27383924250337 39.0 35.0 41.0 29.0 41.0 28-29 36.083926828344175 39.0 35.0 40.0 28.0 41.0 30-31 35.86993953791956 38.5 35.0 40.0 27.0 41.0 32-33 35.61624956984387 38.0 34.5 40.0 27.0 41.0 34-35 35.361489338182594 38.0 34.0 40.0 26.0 41.0 36-37 35.07423137301928 38.0 34.0 40.0 25.0 41.0 38-39 34.82335123746297 38.0 33.5 40.0 25.0 41.0 40-41 34.78282476933671 38.0 33.0 40.0 24.5 41.0 42-43 34.619987289643404 38.0 33.0 40.0 24.0 41.0 44-45 34.338438047948586 38.0 33.0 40.0 23.5 41.0 46-47 33.98388081482213 38.0 33.0 40.0 23.0 41.0 48-49 33.633503664090355 38.0 32.5 40.0 21.5 41.0 50-51 32.88375550804905 36.5 31.5 39.5 20.0 40.5 52-53 32.937874909623275 37.0 31.5 39.0 20.0 40.0 54-55 33.04773160757887 37.0 32.0 40.0 18.5 41.0 56-57 32.735898398393516 37.0 31.0 40.0 17.0 41.0 58-59 32.38927224810429 36.0 31.0 39.0 14.0 41.0 60-61 31.915618700239094 36.0 31.0 39.0 11.0 40.5 62-63 31.3776593022897 35.0 30.0 39.0 8.0 40.0 64-65 30.8700549623318 35.0 29.0 38.0 6.5 40.0 66-67 30.313047802112166 34.5 29.0 38.0 2.0 40.0 68-69 29.761424184910297 34.0 28.0 37.0 2.0 39.5 70-71 29.14030745469836 34.0 27.5 36.5 2.0 39.0 72-73 28.589356072398253 34.0 26.0 36.0 2.0 39.0 74-75 27.980530967920608 33.0 26.0 35.5 2.0 38.0 76-77 27.260731052526115 33.0 25.0 35.0 2.0 37.0 78-79 26.499168787988985 32.0 23.5 35.0 2.0 36.5 80-81 25.856794240263238 32.0 21.0 35.0 2.0 36.0 82-83 25.21551183012339 31.0 19.5 34.5 2.0 35.5 84-85 24.57113053817462 31.0 17.5 34.0 2.0 35.0 86-87 23.909325628477134 31.0 9.5 34.0 2.0 35.0 88-89 23.143356143488752 30.0 4.5 34.0 2.0 35.0 90-91 22.334487251949813 29.0 2.0 34.0 2.0 35.0 92-93 21.496749367314074 29.0 2.0 34.0 2.0 35.0 94-95 20.554349078577268 28.0 2.0 34.0 2.0 35.0 96-97 19.32921639909426 26.0 2.0 33.0 2.0 35.0 98-99 17.876077733947316 24.0 2.0 33.0 2.0 34.0 100-101 14.584247907419856 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5510076529130821 1101 2 0.5676130057607303 1101 3 0.6492958955787209 1101 4 0.6196929572211545 1101 5 0.5751414681538236 1101 6 0.5621307419911261 1101 7 0.4890252909227968 1101 8 0.6233467675083304 1101 9 0.7343000744146622 1101 10-11 0.7222608554601706 1101 12-13 0.7747297465878589 1101 14-15 0.8895248562590083 1101 16-17 0.9300212926634899 1101 18-19 0.836100881184322 1101 20-21 0.930252885091015 1101 22-23 0.9148713926801832 1101 24-25 0.9386526216252662 1101 26-27 0.944662217243625 1101 28-29 0.9973019503747054 1101 30-31 0.9060952402796758 1101 32-33 1.0641512149210612 1101 34-35 1.032610142559271 1101 36-37 1.0447321687677942 1101 38-39 0.9881087716436525 1101 40-41 0.8127968868898279 1101 42-43 1.0487526920022177 1101 44-45 1.0384215420555307 1101 46-47 1.1052430115272927 1101 48-49 1.0200119095844329 1101 50-51 0.8995383326645978 1101 52-53 1.0709422766848178 1101 54-55 1.2447952949216798 1101 56-57 1.154975817280011 1101 58-59 1.1395342078468786 1101 60-61 1.1677177447176241 1101 62-63 0.9799840284621766 1101 64-65 0.9385045824333886 1101 66-67 0.9595891368984084 1101 68-69 1.0321474086935076 1101 70-71 1.3044755061010456 1101 72-73 1.1316672546958557 1101 74-75 1.0187031582586705 1101 76-77 1.1222026972297634 1101 78-79 1.0285165484374836 1101 80-81 1.257121902405192 1101 82-83 1.186466817762252 1101 84-85 1.1328893236947053 1101 86-87 1.4141864608460573 1101 88-89 1.4650583507729813 1101 90-91 1.3542483010170194 1101 92-93 1.227242842607808 1101 94-95 1.3813629308366266 1101 96-97 1.4303479569831907 1101 98-99 1.2809931036266775 1101 100-101 1.0878882646715589 1102 1 0.6551687391881025 1102 2 0.8054719903458647 1102 3 0.6178833299588788 1102 4 0.5949208412479408 1102 5 0.6704428093027914 1102 6 0.6502496089706682 1102 7 0.4992543806851799 1102 8 0.5438075440020498 1102 9 0.6013597490515536 1102 10-11 0.7512699263017666 1102 12-13 0.8025423567271659 1102 14-15 0.8302520075695483 1102 16-17 0.8809894055945406 1102 18-19 0.9915969950057573 1102 20-21 0.7366069261602135 1102 22-23 0.8720683356767154 1102 24-25 0.8363708133928753 1102 26-27 0.8160582735607491 1102 28-29 0.7754211883498101 1102 30-31 0.8151863011415017 1102 32-33 0.7566379982039919 1102 34-35 0.6070747032077648 1102 36-37 0.691999415424192 1102 38-39 0.7983974061944679 1102 40-41 0.9253267056584988 1102 42-43 0.76011350374052 1102 44-45 0.8552890409889216 1102 46-47 0.851649177473611 1102 48-49 0.7454358647681687 1102 50-51 0.7975438604985428 1102 52-53 0.7336913538333434 1102 54-55 0.6956235425241175 1102 56-57 0.7751067787150205 1102 58-59 0.8812760320320763 1102 60-61 0.8096608339977074 1102 62-63 0.6640126693199164 1102 64-65 0.5868673789979724 1102 66-67 0.6868474796612141 1102 68-69 0.45539399089684807 1102 70-71 0.6873089847409766 1102 72-73 0.7280702671798558 1102 74-75 0.8329991014221498 1102 76-77 0.7269108164677398 1102 78-79 0.7675130054298229 1102 80-81 0.9821726360489222 1102 82-83 1.1916618686813898 1102 84-85 0.8244714342884798 1102 86-87 0.7821337423760433 1102 88-89 0.9234617713521054 1102 90-91 0.95937545974747 1102 92-93 0.8894890352658074 1102 94-95 0.9483011044254503 1102 96-97 0.9677880875736307 1102 98-99 1.0474591853570026 1102 100-101 1.063710669093826 1103 1 0.6499133754341422 1103 2 0.7044888956494013 1103 3 0.5017773484253603 1103 4 0.6242638092661394 1103 5 0.582869768578405 1103 6 0.5713567056297464 1103 7 0.4318187234301689 1103 8 0.3489273084875322 1103 9 0.4798280125780252 1103 10-11 0.5773304107123138 1103 12-13 0.5090680715226483 1103 14-15 0.6985507720104209 1103 16-17 0.673159439940406 1103 18-19 0.6932500571405598 1103 20-21 0.6381730085010915 1103 22-23 0.6767616761468531 1103 24-25 0.6509058060330375 1103 26-27 0.6445390264935256 1103 28-29 0.6404383612834792 1103 30-31 0.5632352774562364 1103 32-33 0.8888352416136058 1103 34-35 0.8251883568051639 1103 36-37 0.8675054594047396 1103 38-39 0.7001013180549407 1103 40-41 0.7935246627484673 1103 42-43 0.6688560881555219 1103 44-45 0.6123629515112441 1103 46-47 0.5894782510272947 1103 48-49 0.652595862983965 1103 50-51 0.7377494896523871 1103 52-53 0.6857213284084835 1103 54-55 0.6689988045786279 1103 56-57 0.7327012298502211 1103 58-59 0.7072602418541081 1103 60-61 0.7857922403940698 1103 62-63 0.7383737023722432 1103 64-65 0.5588915549201801 1103 66-67 0.6736479613954565 1103 68-69 0.8413813230704541 1103 70-71 0.8689442048667608 1103 72-73 0.8912381158796201 1103 74-75 0.7361424173595239 1103 76-77 0.7112138629907783 1103 78-79 0.6160606658963914 1103 80-81 0.6804080613578165 1103 82-83 0.6879190612412742 1103 84-85 0.8963501978372612 1103 86-87 0.8165077108848955 1103 88-89 0.7338070142264534 1103 90-91 1.0849943544350111 1103 92-93 1.145330955065976 1103 94-95 1.0292382559487123 1103 96-97 0.9956412478394725 1103 98-99 1.2000989105900643 1103 100-101 0.7305441585447383 1104 1 0.41321573693977953 1104 2 0.44969519060281726 1104 3 0.5274457317575383 1104 4 0.43108428300423185 1104 5 0.38779395560000296 1104 6 0.47251320958526577 1104 7 0.40149558488320025 1104 8 0.4652772003299077 1104 9 0.5293521446868539 1104 10-11 0.47608871498550087 1104 12-13 0.5123802865343663 1104 14-15 0.5965998291273991 1104 16-17 0.5045002609119464 1104 18-19 0.6025103000397394 1104 20-21 0.6662303538536491 1104 22-23 0.729497435392851 1104 24-25 0.7142954121237466 1104 26-27 0.6527556701943382 1104 28-29 0.6900410135188721 1104 30-31 0.5840885294981462 1104 32-33 0.4383752830291243 1104 34-35 0.4789964546422638 1104 36-37 0.6366899021556165 1104 38-39 0.7154976779029312 1104 40-41 0.5687010122810676 1104 42-43 0.4907011058482169 1104 44-45 0.6159027722492851 1104 46-47 0.6071303966025781 1104 48-49 0.6884939305164082 1104 50-51 0.5556418144383173 1104 52-53 0.6405368446013355 1104 54-55 0.771796533140197 1104 56-57 0.7295468248967794 1104 58-59 0.5837380753144572 1104 60-61 0.8159254936676597 1104 62-63 0.80169941463447 1104 64-65 0.8126591096854021 1104 66-67 0.6781913514634326 1104 68-69 0.7811042958527104 1104 70-71 0.6453342805386093 1104 72-73 0.6470792691503924 1104 74-75 0.913137559888952 1104 76-77 0.9155491490632066 1104 78-79 0.8226730356389425 1104 80-81 0.8002053973187486 1104 82-83 0.784340237041679 1104 84-85 0.7041883539999354 1104 86-87 0.909011091612566 1104 88-89 1.0850344685577618 1104 90-91 0.9672172438217075 1104 92-93 0.7449996822444582 1104 94-95 0.34033987279615374 1104 96-97 0.37465302329243855 1104 98-99 0.2654066145435259 1104 100-101 0.35690510992127855 1105 1 0.4878463912649451 1105 2 0.5565368198165324 1105 3 0.5192682247421985 1105 4 0.46184698130358726 1105 5 0.4235943113271503 1105 6 0.5060885932922972 1105 7 0.4336078354245103 1105 8 0.39995443082122506 1105 9 0.5189338505860661 1105 10-11 0.5670599261351583 1105 12-13 0.5287815476500342 1105 14-15 0.7499139479550934 1105 16-17 0.7358546982518632 1105 18-19 0.7685886143263048 1105 20-21 0.7335189040338932 1105 22-23 0.6526589819720598 1105 24-25 0.7159474474587455 1105 26-27 0.8907481217754807 1105 28-29 0.7888114481008941 1105 30-31 0.7900352656360781 1105 32-33 0.7887808196438755 1105 34-35 0.8048710676923463 1105 36-37 0.7786389795622171 1105 38-39 0.8057642400304204 1105 40-41 0.7643415176347048 1105 42-43 0.7787242769956606 1105 44-45 0.7723126501793445 1105 46-47 0.8759679765205846 1105 48-49 0.9805642407824351 1105 50-51 0.7242207461514241 1105 52-53 0.7724167574555381 1105 54-55 0.8464849364160543 1105 56-57 1.0807226670170635 1105 58-59 1.0556339853468657 1105 60-61 1.1135536012880642 1105 62-63 1.014142628166283 1105 64-65 0.9223371469048018 1105 66-67 0.8638069424979804 1105 68-69 0.8638096592685294 1105 70-71 1.1127122736994686 1105 72-73 1.015744337057729 1105 74-75 1.0742380424241667 1105 76-77 0.9996684432192673 1105 78-79 1.1955572914059296 1105 80-81 1.1292692221299987 1105 82-83 1.183377658681227 1105 84-85 1.03195989627552 1105 86-87 1.018796799074753 1105 88-89 1.0330084149149776 1105 90-91 0.8578609393455956 1105 92-93 0.895091761069299 1105 94-95 0.9548137087674071 1105 96-97 0.9708161286939401 1105 98-99 0.9696801487186022 1105 100-101 0.6074758101324971 1106 1 0.2743106500672674 1106 2 0.3267111178716249 1106 3 0.16262640502913328 1106 4 0.28275437530387393 1106 5 0.3595469174253694 1106 6 0.03996257565071204 1106 7 0.2144969204951579 1106 8 0.24971347200954597 1106 9 0.19851249895119594 1106 10-11 0.017660599391561504 1106 12-13 0.14113044049539525 1106 14-15 0.4086398705429204 1106 16-17 0.1461023120577991 1106 18-19 -0.03562834956804295 1106 20-21 0.18653516783022894 1106 22-23 0.1995342128344859 1106 24-25 0.3822344223852454 1106 26-27 0.2222774960952023 1106 28-29 0.18109611809689596 1106 30-31 0.2549412522989982 1106 32-33 0.3352581975884448 1106 34-35 0.3075913966374486 1106 36-37 0.2980348095095664 1106 38-39 0.39268517549729154 1106 40-41 0.5767531742788634 1106 42-43 0.11391636069221533 1106 44-45 0.36308360140643003 1106 46-47 0.12657206654091624 1106 48-49 0.6541550873026338 1106 50-51 0.10161217309053683 1106 52-53 0.2891804479053377 1106 54-55 0.02199932868764165 1106 56-57 -0.23603566742441018 1106 58-59 -0.5276847266993556 1106 60-61 0.18838658001670794 1106 62-63 0.28754823067412616 1106 64-65 0.41312373521368073 1106 66-67 -0.21349826501963776 1106 68-69 -0.3033382786066987 1106 70-71 -0.3851045372716051 1106 72-73 0.17344461483139995 1106 74-75 0.14341442993814013 1106 76-77 0.18132654642893442 1106 78-79 0.4106230348691362 1106 80-81 0.1727167615674574 1106 82-83 0.1547822687884448 1106 84-85 0.3202102607303381 1106 86-87 0.45863297293940164 1106 88-89 0.012698523660414907 1106 90-91 0.15228995191928973 1106 92-93 0.31434300957331374 1106 94-95 0.1671837486111869 1106 96-97 0.06245204448517683 1106 98-99 0.03310467578922527 1106 100-101 0.15022445204578716 1107 1 0.4268845423875405 1107 2 0.4028949461706901 1107 3 0.3908006724516433 1107 4 0.39415691923031915 1107 5 0.3857864469963985 1107 6 0.358951068755033 1107 7 0.3987429791013568 1107 8 0.39038738689235686 1107 9 0.42493288138625473 1107 10-11 0.4863039561335327 1107 12-13 0.4833592317072899 1107 14-15 0.37687780663452486 1107 16-17 0.3851920898167762 1107 18-19 0.42080126940705753 1107 20-21 0.5145072537371007 1107 22-23 0.600694420973916 1107 24-25 0.5495365418538967 1107 26-27 0.5165661767506435 1107 28-29 0.6154329556748124 1107 30-31 0.5822646651652903 1107 32-33 0.46116158055937007 1107 34-35 0.5980585469885753 1107 36-37 0.6136970905824128 1107 38-39 0.5081754171985011 1107 40-41 0.45120321235314975 1107 42-43 0.4339598288367199 1107 44-45 0.5033141976215632 1107 46-47 0.4238943512801825 1107 48-49 0.548873513847461 1107 50-51 0.4873663965161015 1107 52-53 0.5520164182356027 1107 54-55 0.6816848280248706 1107 56-57 0.7018979564016306 1107 58-59 0.5380877230284824 1107 60-61 0.6220826766971932 1107 62-63 0.7193238673608136 1107 64-65 0.5152201125157667 1107 66-67 0.5981142162957589 1107 68-69 0.6236516317072045 1107 70-71 0.6339158606752058 1107 72-73 0.6143571990883885 1107 74-75 0.6499055670901868 1107 76-77 0.4896586458733303 1107 78-79 0.39882617529275066 1107 80-81 0.2682929171925821 1107 82-83 0.2857727995722108 1107 84-85 0.41291321783331014 1107 86-87 0.4177850797166265 1107 88-89 0.5724531207780537 1107 90-91 0.7050827713907601 1107 92-93 0.8071556065217287 1107 94-95 0.950371835589781 1107 96-97 0.9189904219564795 1107 98-99 0.7865357683802507 1107 100-101 0.5056144314574667 1108 1 0.23409477020304337 1108 2 0.3054292327984527 1108 3 0.27968229088759955 1108 4 0.2943025859759487 1108 5 0.3849646557371784 1108 6 0.3045430483594842 1108 7 0.30165289045888954 1108 8 0.17829166318871614 1108 9 0.23383017639122983 1108 10-11 0.24043631132405352 1108 12-13 0.2962812055745232 1108 14-15 0.3089383919630393 1108 16-17 0.3081487796366176 1108 18-19 0.3368182888931557 1108 20-21 0.48049243968308275 1108 22-23 0.5039393713682614 1108 24-25 0.46562562165199495 1108 26-27 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-0.7440116678096516 2316 64-65 -0.7679687058729847 2316 66-67 -0.7248658307181159 2316 68-69 -0.6436304639455628 2316 70-71 -1.1672760774996895 2316 72-73 -0.9258550145952533 2316 74-75 -0.8115793394712902 2316 76-77 -0.5041599289488161 2316 78-79 -0.5218704735731485 2316 80-81 -0.5024500943003858 2316 82-83 -0.44634657721484317 2316 84-85 -0.6214431944746472 2316 86-87 -0.6544677408057389 2316 88-89 -0.6265037976423322 2316 90-91 -0.7219891263447593 2316 92-93 -0.7726609897481715 2316 94-95 -0.7759971799099112 2316 96-97 -0.798172743479018 2316 98-99 -0.7374766611454575 2316 100-101 -0.7478271020135576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9204.0 3 3479.0 4 3810.0 5 4223.0 6 5139.0 7 6187.0 8 7717.0 9 9474.0 10 11613.0 11 14210.0 12 16472.0 13 18910.0 14 20979.0 15 23832.0 16 26394.0 17 30514.0 18 30354.0 19 29814.0 20 32348.0 21 35355.0 22 38614.0 23 43051.0 24 48310.0 25 54079.0 26 60921.0 27 69496.0 28 78370.0 29 89615.0 30 103811.0 31 121360.0 32 142915.0 33 172021.0 34 208674.0 35 258046.0 36 303471.0 37 292992.0 38 128349.0 39 5994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59669879896203 17.421135779395026 13.45177396802737 24.53039145361558 2 16.206713689090048 19.03857133534017 38.92464831856864 25.830066657001133 3 19.6622475795973 22.659111558338786 28.52098984945144 29.157651012612472 4 13.70424866295009 15.233126400800373 34.42419410623319 36.638430830016354 5 15.146495973886442 36.1442326895486 32.78702107461256 15.922250261952398 6 34.918311567860556 33.56770968703917 16.43074647761263 15.083232267487649 7 31.01952811643176 31.13530499176902 19.13803862329065 18.707128268508573 8 27.711198332333698 34.34439184687791 18.630231726687153 19.314178094101237 9 27.41289280638595 16.288508888026612 18.8418505289129 37.45674777667453 10-11 26.595010748973802 25.30851401176555 26.063198846930042 22.03327639233061 12-13 26.886708404222315 22.422890894924365 26.729919043938533 23.96048165691479 14-15 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25.420073714724754 26.72991955534397 24.077447541211306 40-41 23.81178614377573 25.364737211830057 26.928119947771336 23.895356696622873 42-43 24.044997111838182 25.209833697747662 26.651453271846837 24.093715918567316 44-45 23.777006386084167 25.471485666417777 26.63567601731159 24.115831930186467 46-47 23.93900885482609 25.806581408967023 26.191858341533692 24.0625513946732 48-49 24.120188909248128 25.614434576041166 26.21421499316269 24.05116152154801 50-51 23.94373322207951 25.78604264521593 26.21912393863539 24.05110019406917 52-53 24.218153507256478 25.717654168516525 26.072142679819077 23.992049644407924 54-55 23.863564227135388 25.582781691083074 26.281805249630203 24.27184883215133 56-57 23.83474793522813 25.96877035596013 26.26114420149947 23.935337507312276 58-59 24.068585849016984 25.382369917075465 26.21724060811979 24.331803625787764 60-61 23.88586114717012 25.50416099087172 26.645029956868516 23.96494790508964 62-63 23.72817267875425 25.71478459100396 26.406669122360345 24.15037360788145 64-65 23.57831857828049 25.546212348931363 26.47300037246523 24.402468700322913 66-67 23.663528753225787 25.676021162996044 26.40290589833848 24.257544185439688 68-69 23.532979218975687 25.465509984247475 26.571057743297366 24.43045305347947 70-71 23.57820328770131 25.636127481594073 26.3814090611722 24.404260169532417 72-73 23.841852606081915 25.550414853665803 26.199549740930113 24.40818279932217 74-75 23.567559563866055 25.863812552339176 26.167704545956216 24.40092333783855 76-77 23.829621081548176 25.62828832742404 26.155807535865915 24.386283055161865 78-79 23.90688353997234 25.67128381809517 26.13989343833251 24.281939203599972 80-81 23.655174515103443 26.047381638677503 26.184335887130324 24.11310795908873 82-83 23.895895495496198 25.918743502208685 25.95011063457115 24.235250367723964 84-85 23.814881864650058 25.49434894454452 26.042628374085474 24.648140816719945 86-87 23.936752115050133 25.38571893149318 26.195507489958214 24.482021463498477 88-89 23.86285958986736 25.56170089648262 26.030816309267262 24.54462320438276 90-91 23.78323431605596 25.662507934504607 26.12512691788381 24.42913083155562 92-93 23.96145519925831 25.21631588467893 26.2005253232285 24.62170359283426 94-95 23.786011349836595 25.24357918394331 26.27779436196633 24.692615104253765 96-97 23.986263130830825 25.205744352629132 26.096984109369796 24.711008407170244 98-99 24.06063550424785 25.226037221984228 25.826211585881254 24.88711568788667 100-101 24.617798774359315 24.623782278659228 25.9424019272359 24.816017019745566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 725.0 1 494.0 2 396.0 3 511.0 4 422.5 5 429.5 6 757.5 7 1536.0 8 2320.0 9 2505.0 10 2127.5 11 1742.5 12 1732.0 13 1859.0 14 1976.0 15 2086.0 16 2144.0 17 2208.5 18 2350.0 19 2494.0 20 2656.0 21 3014.5 22 3635.5 23 4263.0 24 5112.0 25 6310.5 26 7509.5 27 9152.0 28 10992.5 29 12931.0 30 15371.0 31 18134.0 32 21925.5 33 25177.5 34 27112.0 35 29637.0 36 33785.0 37 38843.5 38 44366.0 39 49084.0 40 53515.5 41 62429.0 42 71688.5 43 79174.0 44 86486.5 45 92834.5 46 97487.5 47 101425.0 48 107469.5 49 109185.0 50 107812.5 51 106723.5 52 106120.0 53 107419.0 54 107861.0 55 104076.0 56 95920.0 57 88334.5 58 81293.5 59 72231.5 60 61728.0 61 52051.0 62 43987.0 63 35440.0 64 28145.5 65 22923.0 66 17924.5 67 14448.5 68 11092.0 69 7684.0 70 5853.5 71 4654.0 72 3796.5 73 3157.0 74 2463.5 75 2003.0 76 1462.0 77 884.5 78 555.0 79 365.0 80 214.5 81 119.0 82 64.5 83 38.5 84 26.5 85 17.0 86 16.5 87 14.5 88 10.5 89 7.0 90 4.5 91 4.0 92 3.5 93 2.0 94 1.5 95 0.5 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18530403102670698 2 0.04671661490470943 3 0.04460733630533292 4 0.05124765782188861 5 0.05648179360552662 6 0.06070035080427965 7 0.03452967188608958 8 0.03327972901238498 9 0.03906071480326876 10-11 0.040447370178784796 12-13 0.03714673977790859 14-15 0.032303211142303265 16-17 0.031111859340803565 18-19 0.038611516583031164 20-21 0.048142330995028744 22-23 0.04511512559777541 24-25 0.06456736156980325 26-27 0.05021254887960199 28-29 0.04255664877816131 30-31 0.050837520316454284 32-33 0.047419707771168274 34-35 0.04640412918628328 36-37 0.044236259514701864 38-39 0.02560429855354267 40-41 0.02355361102637106 42-43 0.020721709203134077 44-45 0.003808419693318704 46-47 0.010546392996882564 48-49 0.010292498350661318 50-51 0.008007446534670096 52-53 0.009042555476956718 54-55 0.010175316206251511 56-57 0.008183219751284805 58-59 0.010136255491448243 60-61 0.010546392996882564 62-63 0.0031248571842615006 64-65 0.005722394718678873 66-67 0.003222508971269673 68-69 0.008183219751284805 70-71 0.021385741354789645 72-73 0.01872961274816737 74-75 0.019159280611003325 76-77 0.01191351801499697 78-79 0.00378888933591707 80-81 0.005390378642851089 82-83 0.004394330415367735 84-85 0.00423808755615466 86-87 0.007402005455219429 88-89 0.028338548589771483 90-91 0.03451014152868795 92-93 0.021483393141797816 94-95 0.030662661120565975 96-97 0.034100004023253624 98-99 0.02060452705872427 100-101 0.12058042659769065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2560117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.85838662951364 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7510934213171 35.29608343306773 2 13.289320107609331 12.720108395066742 3 5.1074303952292635 7.333001356146353 4 2.4604382637125664 4.71010422811221 5 1.3447557767410672 3.2178920942772957 6 0.8909291312357096 2.5583058493305173 7 0.5873675815084614 1.9677325366632015 8 0.43743344915270765 1.6747887307385583 9 0.30155511936879625 1.2988747343574887 >10 1.6136542216686034 13.893374415334362 >50 0.12406297720835655 4.148334852409845 >100 0.08497585153416684 7.8344322470423515 >500 0.005258318090312542 1.6813281100906983 >1k 0.001643224403221582 1.4246010764647143 >5k 8.21612201610791E-5 0.24103794089783878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6130 0.23944218174403747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4764 0.18608524532277237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.13007218029488496 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2853 0.11144021933372576 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2730 0.1066357514129237 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9530357401634378E-5 0.0 22-23 0.0 0.0 0.0 7.812142960653751E-5 0.0 24-25 0.0 0.0 0.0 3.1248571842615005E-4 0.0 26-27 0.0 0.0 0.0 8.78866083073547E-4 0.0 28-29 0.0 0.0 0.0 0.0030467357546549627 1.9530357401634378E-5 30-31 0.0 0.0 0.0 0.011659623368775723 3.9060714803268756E-5 32-33 0.0 0.0 0.0 0.028651034308197633 3.9060714803268756E-5 34-35 0.0 0.0 0.0 0.056188838244502104 3.9060714803268756E-5 36-37 0.0 0.0 0.0 0.10136255491448243 3.9060714803268756E-5 38-39 0.0 0.0 0.0 0.16977739689240764 3.9060714803268756E-5 40-41 0.0 0.0 0.0 0.2420201889210532 3.9060714803268756E-5 42-43 0.0 0.0 0.0 0.3198681935239679 3.9060714803268756E-5 44-45 0.0 0.0 0.0 0.4155669447919763 3.9060714803268756E-5 46-47 0.0 0.0 0.0 0.5287844266492508 3.9060714803268756E-5 48-49 0.0 0.0 0.0 0.6498140514671791 3.9060714803268756E-5 50-51 0.0 0.0 0.0 0.7780699085237119 3.9060714803268756E-5 52-53 0.0 0.0 0.0 0.9076733602409577 3.9060714803268756E-5 54-55 0.0 0.0 0.0 1.0487216013955611 3.9060714803268756E-5 56-57 0.0 0.0 0.0 1.215393671461109 7.812142960653751E-5 58-59 0.0 0.0 0.0 1.4021429489355368 7.812142960653751E-5 60-61 0.0 0.0 0.0 1.6036376462481987 7.812142960653751E-5 62-63 0.0 0.0 0.0 1.797203018455797 7.812142960653751E-5 64-65 0.0 0.0 0.0 2.0011390104436635 7.812142960653751E-5 66-67 0.0 0.0 0.0 2.2377688207218656 7.812142960653751E-5 68-69 0.0 0.0 0.0 2.497639756308012 1.1718214440980627E-4 70-71 0.0 0.0 0.0 2.7868062279966113 1.1718214440980627E-4 72-73 0.0 0.0 0.0 3.0758750478982018 1.1718214440980627E-4 74-75 0.0 0.0 0.0 3.3656664910236525 1.1718214440980627E-4 76-77 0.0 0.0 0.0 3.6915500346273236 1.3671250181144064E-4 78-79 0.0 0.0 0.0 4.042373844632882 1.5624285921307502E-4 80-81 0.0 0.0 0.0 4.417864496036705 1.5624285921307502E-4 82-83 0.0 0.0 0.0 4.79925331537582 1.5624285921307502E-4 84-85 0.0 0.0 0.0 5.188005079455353 1.5624285921307502E-4 86-87 0.0 0.0 0.0 5.596150488434708 1.5624285921307502E-4 88-89 0.0 0.0 0.0 6.035290574610457 1.5624285921307502E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7295 0.0 35.239357 1 GGTATCA 4965 0.0 32.696003 1 GTACATG 9335 0.0 30.649237 1 TACATGG 9595 0.0 29.621725 2 ATCAACG 9225 0.0 27.667 3 ACATGGG 9975 0.0 27.25444 3 TATCAAC 9365 0.0 27.253399 2 TCAACGC 9355 0.0 27.18092 4 CAACGCA 9525 0.0 26.592884 5 AACGCAG 9880 0.0 25.73005 6 CATGGGG 6370 0.0 23.57772 4 ACGCAGA 11030 0.0 23.002079 7 CGCAGAG 11390 0.0 22.14992 8 GAGTACT 5370 0.0 21.58316 12-13 GCAGAGT 12110 0.0 20.477894 9 AGTACTT 5770 0.0 20.37506 12-13 GTACTTT 5695 0.0 20.018614 14-15 CATGGGA 4650 0.0 19.93131 4 ATGGGGA 3815 0.0 19.308138 5 GTGTAGG 1295 0.0 19.115828 1 >>END_MODULE