FastQCFastQC Report
Thu 26 May 2016
SRR938558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560117
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.2424107960690859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51170.19987367764832623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.13956393399207925No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29710.11604938368051149No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC27870.10886221215671002No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG26700.10429210852472759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.038.678791
GTATCAA74150.035.7275921
ATCAACG94600.027.787373
GTACATG92950.027.7338161
TATCAAC94850.027.6797072
TCAACGC95450.027.639524
CAACGCA97850.026.913025
TACATGG95200.026.828542
AACGCAG100250.026.436686
ACATGGG99150.024.6903923
ACGCAGA114300.022.9792587
GAGTACT56500.022.15079312-13
CGCAGAG119400.021.9579548
GTACTTT61450.020.36647214-15
TACACCG3553.6379788E-1220.085215
CATGGGG64200.020.0653974
TAAGGTG9500.020.0147365
GCAGAGT127800.019.8085849
CATGGGA45750.019.637414
AGTACTT60700.019.52265712-13
Produced by FastQC (version 0.11.5)