FastQCFastQC Report
Thu 26 May 2016
SRR938557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556923
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61030.2386853260735658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.18541817645662384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.12960890883299966No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG28390.11103189263032168No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27210.10641697071049852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.041.136961
GTATCAA68950.037.81531
GTACATG94800.029.2134111
ATCAACG89800.028.7394183
TATCAAC91200.028.4024722
TCAACGC90900.028.3916364
TACATGG97950.027.8523562
CAACGCA92850.027.8465565
AACGCAG96100.026.950056
ACATGGG102750.025.7648643
ACGCAGA111700.023.1368777
CGCAGAG115150.022.4024168
GTATAGG4400.021.666651
CATGGGG67450.021.491784
GAGTACT56950.020.4348712-13
GCAGAGT124950.019.9602079
GGACCGA7150.019.2742926
AGTACTT60350.019.24425512-13
ATTCGCG5000.018.995880-81
GTACTTT60850.018.9309314-15
Produced by FastQC (version 0.11.5)