##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938557_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2556923 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.018273917517266 31.0 31.0 34.0 28.0 34.0 2 31.151912278938397 31.0 31.0 34.0 27.0 34.0 3 31.21606829771565 31.0 31.0 34.0 27.0 34.0 4 34.876363113007315 37.0 35.0 37.0 32.0 37.0 5 34.86722204775036 37.0 35.0 37.0 32.0 37.0 6 34.87361645227487 37.0 35.0 37.0 32.0 37.0 7 34.7719419004796 37.0 35.0 37.0 32.0 37.0 8 34.7292155454036 37.0 35.0 37.0 31.0 37.0 9 36.20102560773242 39.0 35.0 39.0 32.0 39.0 10-11 36.04793789253724 39.0 35.0 39.0 30.0 39.0 12-13 35.86413845860825 38.0 35.0 39.0 30.0 39.0 14-15 36.84493021494977 39.0 36.0 41.0 30.5 41.0 16-17 36.670900336068 39.0 36.0 41.0 30.0 41.0 18-19 36.498021645548185 39.0 36.0 40.0 30.0 41.0 20-21 36.46608364819746 39.0 36.0 40.5 29.5 41.0 22-23 36.547832101318654 39.0 36.0 41.0 30.0 41.0 24-25 36.45036847022769 39.0 36.0 41.0 29.5 41.0 26-27 36.25094772114765 39.0 35.0 40.5 29.0 41.0 28-29 36.05013838899333 39.0 35.0 40.0 27.5 41.0 30-31 35.79865897408721 38.0 35.0 40.0 27.0 41.0 32-33 35.52326389961684 38.0 34.0 40.0 27.0 41.0 34-35 35.25188889145274 38.0 34.0 40.0 26.0 41.0 36-37 34.9644271649948 38.0 34.0 40.0 25.0 41.0 38-39 34.64624159585564 38.0 33.0 40.0 24.5 41.0 40-41 34.5582354259397 38.0 33.0 40.0 24.0 41.0 42-43 34.34206876781194 38.0 33.0 40.0 24.0 41.0 44-45 34.04875645453539 38.0 33.0 40.0 23.0 41.0 46-47 33.65613395475734 38.0 32.5 40.0 21.5 41.0 48-49 33.27662780615607 37.0 32.0 40.0 19.5 41.0 50-51 32.482291410417915 36.5 30.5 39.5 17.5 40.5 52-53 32.49647740663289 36.5 31.0 39.0 17.5 40.0 54-55 32.55153264294623 37.0 31.0 39.0 15.0 41.0 56-57 32.20017771360342 36.0 31.0 39.0 12.0 41.0 58-59 31.743408385782445 36.0 30.5 39.0 8.0 41.0 60-61 31.236737477037828 35.0 29.5 39.0 7.0 40.0 62-63 30.635548469781845 35.0 28.5 38.5 2.0 40.0 64-65 30.113915241092517 34.5 28.0 38.0 2.0 40.0 66-67 29.534834838593106 34.0 27.5 37.5 2.0 40.0 68-69 28.905964708362355 34.0 26.5 37.0 2.0 39.0 70-71 28.265613982118353 34.0 26.0 36.0 2.0 39.0 72-73 27.62177136347086 33.0 25.5 36.0 2.0 39.0 74-75 26.91085261464659 32.5 23.5 35.0 2.0 38.0 76-77 26.173596545535396 32.0 21.5 35.0 2.0 37.0 78-79 25.387964752947195 31.5 19.0 35.0 2.0 36.5 80-81 24.636527771857033 31.0 15.5 34.5 2.0 36.0 82-83 23.921785090908095 31.0 7.5 34.0 2.0 35.5 84-85 23.199698426585392 30.0 4.0 34.0 2.0 35.0 86-87 22.47185601600048 29.5 2.0 34.0 2.0 35.0 88-89 21.71390026215103 29.0 2.0 34.0 2.0 35.0 90-91 20.904639873785797 29.0 2.0 34.0 2.0 35.0 92-93 20.03070507011748 27.0 2.0 34.0 2.0 35.0 94-95 19.12961653518702 25.5 2.0 33.0 2.0 35.0 96-97 18.060724550563314 24.5 2.0 33.0 2.0 35.0 98-99 16.79540330311081 11.0 2.0 32.5 2.0 34.0 100-101 14.056155191220071 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6545250934061819 1101 2 0.6048487610324962 1101 3 0.6911283560966268 1101 4 0.5315174410587318 1101 5 0.5788886460440921 1101 6 0.5202428926000664 1101 7 0.5918484662501484 1101 8 0.3673633575385935 1101 9 0.5828773259617321 1101 10-11 0.7599876079204435 1101 12-13 0.8971821794284054 1101 14-15 1.125578650601895 1101 16-17 1.0583952081814516 1101 18-19 1.17034518443446 1101 20-21 1.1545464289431209 1101 22-23 1.0131581591296381 1101 24-25 1.1526407397196579 1101 26-27 1.0549286952796777 1101 28-29 1.1471545500287377 1101 30-31 1.2550109917670298 1101 32-33 1.3346509666005488 1101 34-35 1.4439598266563465 1101 36-37 1.349038673757974 1101 38-39 1.4506540890372719 1101 40-41 1.502707562909407 1101 42-43 1.6296893980453788 1101 44-45 1.6060016263431223 1101 46-47 1.7739570007434367 1101 48-49 1.805817841801705 1101 50-51 1.7804863447975308 1101 52-53 1.888765188512913 1101 54-55 2.095831447063226 1101 56-57 2.023216645312324 1101 58-59 2.2327629681424206 1101 60-61 2.078984728630786 1101 62-63 2.023441498720061 1101 64-65 2.239356641944042 1101 66-67 2.450166899209119 1101 68-69 2.47539821467608 1101 70-71 2.500894210771744 1101 72-73 2.856648398193176 1101 74-75 3.030235984819324 1101 76-77 3.2035664950243223 1101 78-79 3.4374044826159853 1101 80-81 3.6389152348067775 1101 82-83 3.782662471767619 1101 84-85 3.87951117415194 1101 86-87 4.163058697214165 1101 88-89 4.454882742449325 1101 90-91 4.657843880258053 1101 92-93 4.687246852704895 1101 94-95 5.06178680957122 1101 96-97 5.091948038029674 1101 98-99 5.317049029134399 1101 100-101 5.367729275990859 1102 1 0.5548256061770438 1102 2 0.4521301817466501 1102 3 0.4208169325151232 1102 4 0.43250611690339014 1102 5 0.3538064425816785 1102 6 0.3095383819294426 1102 7 0.3306347223525705 1102 8 0.49578695021158836 1102 9 0.45667760226963594 1102 10-11 0.5817829762254618 1102 12-13 0.5587696092808301 1102 14-15 0.8174227265530547 1102 16-17 0.9427367053829983 1102 18-19 0.9261158116385033 1102 20-21 0.8767324266120013 1102 22-23 0.6729428847395056 1102 24-25 0.41913822055126104 1102 26-27 0.7146655697234294 1102 28-29 0.5748938896454021 1102 30-31 0.5624424454370427 1102 32-33 0.6698176636784652 1102 34-35 0.726374988586926 1102 36-37 0.8628704536351606 1102 38-39 1.0856103899373295 1102 40-41 1.2354258442891606 1102 42-43 1.1759936867759606 1102 44-45 1.243619345797505 1102 46-47 1.2581059761326685 1102 48-49 1.2314683011356564 1102 50-51 1.2288162087661547 1102 52-53 1.363792465539511 1102 54-55 1.6282797510465983 1102 56-57 1.538547823791582 1102 58-59 1.6004563557443099 1102 60-61 1.6356842642696634 1102 62-63 1.9083255573926579 1102 64-65 1.8161721002911833 1102 66-67 1.8830404641396186 1102 68-69 2.1668149815757367 1102 70-71 2.1655677615516993 1102 72-73 2.23844223972948 1102 74-75 2.408370608582551 1102 76-77 2.4248066408493507 1102 78-79 2.9103605675341164 1102 80-81 3.3233494552796294 1102 82-83 3.4004383421188855 1102 84-85 3.4585976078808685 1102 86-87 3.8072642375227232 1102 88-89 3.84917020693333 1102 90-91 4.216765981538675 1102 92-93 4.26548611598313 1102 94-95 4.655828873911489 1102 96-97 4.731206847671405 1102 98-99 4.85270820222156 1102 100-101 4.8017358150295895 1103 1 0.5368501076988466 1103 2 0.5956970145520089 1103 3 0.44232792241367136 1103 4 0.406128301264431 1103 5 0.31043334554657775 1103 6 0.4948137888473383 1103 7 0.4664130718743422 1103 8 0.42123329335573345 1103 9 0.6082635054628156 1103 10-11 0.4908400613559536 1103 12-13 0.5764685452429319 1103 14-15 0.7117611119746883 1103 16-17 0.727684941329521 1103 18-19 0.9592426047239471 1103 20-21 0.8385172737552935 1103 22-23 0.8134701925365491 1103 24-25 0.9321021328706465 1103 26-27 0.8371982965836864 1103 28-29 0.941498539800925 1103 30-31 1.0576499081516957 1103 32-33 1.1064007959211395 1103 34-35 0.9723839145622577 1103 36-37 1.1902144097146206 1103 38-39 1.2660591774112788 1103 40-41 1.2381735408145147 1103 42-43 1.285290642848679 1103 44-45 1.3379331991654126 1103 46-47 1.5856415555985919 1103 48-49 1.469565906047464 1103 50-51 1.3704064538843355 1103 52-53 1.4895610448424819 1103 54-55 1.7520366841919568 1103 56-57 1.720332117099801 1103 58-59 1.9889855969938104 1103 60-61 2.0655330637433806 1103 62-63 1.9989641292366116 1103 64-65 2.324740656730704 1103 66-67 2.3808370170843 1103 68-69 2.5624349981694863 1103 70-71 2.6725638346479883 1103 72-73 2.8720305442947485 1103 74-75 3.013740305922333 1103 76-77 3.2691154351468406 1103 78-79 3.2569199381218503 1103 80-81 3.5465333643825154 1103 82-83 3.8881438246956286 1103 84-85 4.1524648252110765 1103 86-87 4.312983117227969 1103 88-89 4.634458199398335 1103 90-91 4.848154655540345 1103 92-93 4.772868986077206 1103 94-95 4.91022405228011 1103 96-97 5.278555486497446 1103 98-99 5.407441397498484 1103 100-101 5.360973979378729 1104 1 0.6163596187232017 1104 2 0.370121906620966 1104 3 0.5196735414931908 1104 4 0.40403701515493395 1104 5 0.3919921435204188 1104 6 0.4626460030532584 1104 7 0.3865585556043385 1104 8 0.5782356755752929 1104 9 0.599250009619368 1104 10-11 0.5827506320514217 1104 12-13 0.6532684302978709 1104 14-15 0.6804226820631314 1104 16-17 0.7843837223336578 1104 18-19 1.0312032343372621 1104 20-21 0.871645003913244 1104 22-23 0.8641961658713271 1104 24-25 0.9017070687071609 1104 26-27 0.9177976603049203 1104 28-29 0.9614690553703653 1104 30-31 1.0065653266044592 1104 32-33 1.0010851815856583 1104 34-35 1.086988059726309 1104 36-37 1.2929033761782023 1104 38-39 1.3098195815554234 1104 40-41 1.4852745785508432 1104 42-43 1.4082622279513899 1104 44-45 1.359310941651053 1104 46-47 1.4329958635731614 1104 48-49 1.561392223367271 1104 50-51 1.6559995517389794 1104 52-53 1.6632207700285377 1104 54-55 1.8630486258964467 1104 56-57 1.8290647966972173 1104 58-59 1.9241450152629334 1104 60-61 2.068771420028881 1104 62-63 2.1736234174273648 1104 64-65 2.28431731539996 1104 66-67 2.181323153110565 1104 68-69 2.556869259281836 1104 70-71 2.6888982771899066 1104 72-73 2.7805386049027696 1104 74-75 3.0067934408110517 1104 76-77 3.2073065296245424 1104 78-79 3.337078990811243 1104 80-81 3.5261211774845513 1104 82-83 3.883031659732449 1104 84-85 4.229223136198705 1104 86-87 4.264636217769198 1104 88-89 4.466205398462449 1104 90-91 4.6410841734682045 1104 92-93 4.851698688835377 1104 94-95 5.029671023664569 1104 96-97 4.881224643640618 1104 98-99 5.049057804036753 1104 100-101 5.054064862500338 1105 1 0.6208214175541116 1105 2 0.5853151023014078 1105 3 0.4467082397299116 1105 4 0.4182080199717646 1105 5 0.49776527179412255 1105 6 0.4460352407978192 1105 7 0.5102854377775543 1105 8 0.40852016929348167 1105 9 0.6235079167317963 1105 10-11 0.6255472401778377 1105 12-13 0.7078922367373508 1105 14-15 0.8925243129075753 1105 16-17 0.8701721352537675 1105 18-19 0.8627668327705393 1105 20-21 0.880503428871414 1105 22-23 0.8016742474312295 1105 24-25 0.7577821933067597 1105 26-27 0.9064298853540578 1105 28-29 0.9793932364524451 1105 30-31 0.8732036526544107 1105 32-33 1.0203318622779776 1105 34-35 1.2771975284924366 1105 36-37 1.0764634612321942 1105 38-39 1.0940325186987252 1105 40-41 1.0162639178716049 1105 42-43 1.2332416957371137 1105 44-45 1.2206440006857875 1105 46-47 1.3658052973019252 1105 48-49 1.2566711802182837 1105 50-51 1.238537928094864 1105 52-53 1.505253708361181 1105 54-55 1.7219620177218147 1105 56-57 1.7691749393699965 1105 58-59 1.76409226635705 1105 60-61 1.9294095178538342 1105 62-63 2.1573396437429935 1105 64-65 2.285801983340619 1105 66-67 2.4309113034111967 1105 68-69 2.5313671240922737 1105 70-71 2.6161465747438832 1105 72-73 2.8545011052844806 1105 74-75 2.8520504591317355 1105 76-77 3.1038269822815145 1105 78-79 3.3450212209552674 1105 80-81 3.5323169238204954 1105 82-83 3.8895264290403624 1105 84-85 3.99698506858946 1105 86-87 4.260813207867127 1105 88-89 4.513386658939506 1105 90-91 4.767473252098146 1105 92-93 4.7184840753633495 1105 94-95 4.971228707534365 1105 96-97 5.29580331938914 1105 98-99 5.709382012120091 1105 100-101 5.511211760649305 1106 1 0.2973864351265121 1106 2 0.35195892487113056 1106 3 0.2648659502787609 1106 4 0.3242796066362459 1106 5 0.3658406912550376 1106 6 0.07657212571416494 1106 7 0.3495584213524836 1106 8 0.4070619747045967 1106 9 0.42671001308080747 1106 10-11 0.23756121562643528 1106 12-13 0.15442388585645972 1106 14-15 0.5696932901269705 1106 16-17 0.3915517199268308 1106 18-19 0.6598316332949494 1106 20-21 0.5590558194366437 1106 22-23 0.4619583654019337 1106 24-25 0.4327429430668346 1106 26-27 0.5435857429458295 1106 28-29 0.49346725671903613 1106 30-31 0.6066211300856281 1106 32-33 0.8346760466366661 1106 34-35 0.7534458003161433 1106 36-37 0.7168354673853514 1106 38-39 0.8022798033783047 1106 40-41 0.95824620288505 1106 42-43 0.5601417538967723 1106 44-45 0.6868447027571492 1106 46-47 1.1154271484604479 1106 48-49 1.1855693646438965 1106 50-51 0.4773663003046664 1106 52-53 0.9851623753248546 1106 54-55 1.0374058749303998 1106 56-57 1.1096222977364576 1106 58-59 0.45483304792334067 1106 60-61 1.7271182459430463 1106 62-63 1.926331422881546 1106 64-65 2.1230448504550594 1106 66-67 2.142655384392544 1106 68-69 1.6496239455611352 1106 70-71 2.1525326851311455 1106 72-73 2.3416145445444307 1106 74-75 2.517100909904819 1106 76-77 2.736565750543246 1106 78-79 3.045854694784289 1106 80-81 3.292797360397042 1106 82-83 3.3611672088144395 1106 84-85 3.4835118359128927 1106 86-87 3.7875940125450214 1106 88-89 3.721752102741206 1106 90-91 4.091758425094998 1106 92-93 4.448633541101728 1106 94-95 4.439154056980055 1106 96-97 4.6126952999057345 1106 98-99 4.740997031619521 1106 100-101 4.289572051444454 1107 1 0.49305989483868373 1107 2 0.4466586036255009 1107 3 0.48471077846025423 1107 4 0.546477615490268 1107 5 0.5049648757913943 1107 6 0.42344805566817456 1107 7 0.4791443324006437 1107 8 0.39182728214935736 1107 9 0.550886179384193 1107 10-11 0.5572220670491745 1107 12-13 0.5274773814074081 1107 14-15 0.758384107685373 1107 16-17 0.8369693325216048 1107 18-19 0.85649597278789 1107 20-21 0.9227976307968646 1107 22-23 0.8728387470285313 1107 24-25 0.8818066853564801 1107 26-27 1.0048359090390733 1107 28-29 1.032142955868828 1107 30-31 0.9650815126060337 1107 32-33 1.0718718456471734 1107 34-35 1.0905951305848447 1107 36-37 1.1594443292688652 1107 38-39 1.0950282433722691 1107 40-41 1.181191537918096 1107 42-43 1.387690110010844 1107 44-45 1.5619586513162957 1107 46-47 1.3601062234320622 1107 48-49 1.5239983415289444 1107 50-51 1.6370540614484597 1107 52-53 1.6519625891797176 1107 54-55 1.7538872869105475 1107 56-57 1.9151364140553895 1107 58-59 2.098213030851422 1107 60-61 2.021712133203515 1107 62-63 2.0671060772842935 1107 64-65 2.1352850070109284 1107 66-67 2.2329058567400537 1107 68-69 2.4369154097958194 1107 70-71 2.308849930474892 1107 72-73 2.5176202475399663 1107 74-75 2.702179601109492 1107 76-77 2.9899860009005046 1107 78-79 3.1733350890556444 1107 80-81 3.475888986024291 1107 82-83 3.5948295001852593 1107 84-85 3.7805713764358373 1107 86-87 3.9947268224212067 1107 88-89 4.111661988655882 1107 90-91 4.386519937617418 1107 92-93 4.52205512468889 1107 94-95 4.554107470908033 1107 96-97 4.8299920447472005 1107 98-99 4.8101258241690985 1107 100-101 4.939562059693442 1108 1 0.3221372366019395 1108 2 0.4423969309473783 1108 3 0.2939707320771632 1108 4 0.2741139336777678 1108 5 0.3539395620364232 1108 6 0.3240206692766634 1108 7 0.2802601020203497 1108 8 0.37899681525065176 1108 9 0.22415008536901126 1108 10-11 0.3973670489264336 1108 12-13 0.4475582888023979 1108 14-15 0.40299524004803544 1108 16-17 0.5745698933942549 1108 18-19 0.5103138667632621 1108 20-21 0.4777179543086163 1108 22-23 0.5575260988199986 1108 24-25 0.6090392240500861 1108 26-27 0.5335726368134246 1108 28-29 0.6493492646761894 1108 30-31 0.6220294867018552 1108 32-33 0.723206884996209 1108 34-35 0.858156592809479 1108 36-37 0.9070322780816582 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24-25 -0.1023517053977443 2311 26-27 0.08811521232723152 2311 28-29 0.11756781088200086 2311 30-31 0.1711021736953029 2311 32-33 0.1589943619418861 2311 34-35 0.20736411897844675 2311 36-37 0.2163137464747109 2311 38-39 0.3013216630344431 2311 40-41 0.16948825751185126 2311 42-43 0.4678744232628915 2311 44-45 0.484273879740428 2311 46-47 0.5426785901567044 2311 48-49 0.6164642429811309 2311 50-51 0.6250474675472191 2311 52-53 0.7641237462722543 2311 54-55 0.8411320228936674 2311 56-57 0.8953562008267468 2311 58-59 0.8041219056403257 2311 60-61 0.9926357712364933 2311 62-63 1.1638519035564592 2311 64-65 1.1080635523879678 2311 66-67 0.8979546030194321 2311 68-69 0.7758611606494945 2311 70-71 1.1485394328469987 2311 72-73 1.2645591660149655 2311 74-75 1.3536723953645726 2311 76-77 1.6400837021270256 2311 78-79 1.7465275418408375 2311 80-81 1.6162590433930184 2311 82-83 1.7140220155707802 2311 84-85 2.0776643364327327 2311 86-87 2.190665849701535 2311 88-89 2.2686300759028555 2311 90-91 2.529765883873548 2311 92-93 2.5496477098290597 2311 94-95 2.758204662389023 2311 96-97 2.6914545374624588 2311 98-99 2.8429604415544 2311 100-101 2.9745161453336717 2312 1 -0.4523008677576996 2312 2 -0.3313452624982922 2312 3 -0.2593008552169991 2312 4 -0.28143450113208957 2312 5 -0.2529584484840228 2312 6 -0.21313042346729816 2312 7 -0.19417749121827654 2312 8 -0.1965949023348088 2312 9 -0.1761224076577932 2312 10-11 -0.3765035145268101 2312 12-13 -0.34173423715370177 2312 14-15 -0.27053604389973174 2312 16-17 -0.10087121386683862 2312 18-19 -0.12635831869974368 2312 20-21 -0.23884926000455664 2312 22-23 -0.22197072744616975 2312 24-25 -0.35555122975591047 2312 26-27 -0.16378114555413248 2312 28-29 -0.27036050508738896 2312 30-31 -0.11356735257823658 2312 32-33 -0.11097700612086214 2312 34-35 -0.22673254700924872 2312 36-37 -0.032132461069039664 2312 38-39 0.08679968675348704 2312 40-41 -0.020116008529065255 2312 42-43 0.05498345591307441 2312 44-45 0.07305744460081343 2312 46-47 -0.09458431209656482 2312 48-49 0.15318906685932632 2312 50-51 0.2524688930270216 2312 52-53 0.3450978068789965 2312 54-55 0.4220418999562199 2312 56-57 0.5038876623468127 2312 58-59 0.8141606803498824 2312 60-61 0.7143314928779034 2312 62-63 0.7411618053809548 2312 64-65 0.7210155763913448 2312 66-67 0.927559357716131 2312 68-69 0.989097946909812 2312 70-71 1.155327443968602 2312 72-73 1.1279682287567212 2312 74-75 1.2025730654433033 2312 76-77 1.2879226472584513 2312 78-79 1.4036179688588923 2312 80-81 1.5871485779433243 2312 82-83 1.9200059347445837 2312 84-85 1.9034521577887915 2312 86-87 2.138040027484383 2312 88-89 2.07931121775216 2312 90-91 2.0429114839270177 2312 92-93 2.271286273935573 2312 94-95 2.348276392246941 2312 96-97 2.60706305126401 2312 98-99 2.6014154208491966 2312 100-101 2.4272235072368264 2313 1 -0.33206233641381644 2313 2 -0.38333997733093383 2313 3 -0.42569597102695766 2313 4 -0.3552987690447367 2313 5 -0.40904108419157836 2313 6 -0.25655762050607933 2313 7 -0.30873445547814526 2313 8 -0.3970190354325922 2313 9 -0.40474935862682315 2313 10-11 -0.3229590545112657 2313 12-13 -0.3609728940160508 2313 14-15 -0.39935548492011463 2313 16-17 -0.3749648238569989 2313 18-19 -0.29211658961776976 2313 20-21 -0.2618392875565334 2313 22-23 -0.2598740772551693 2313 24-25 -0.17303934841675783 2313 26-27 -0.23694331358306187 2313 28-29 -0.16672309931906426 2313 30-31 -0.21870397960858412 2313 32-33 -0.2362605640178046 2313 34-35 -0.18939506511415516 2313 36-37 -0.05614708938768587 2313 38-39 0.09164833960922891 2313 40-41 0.1966204115845187 2313 42-43 0.25010738622123085 2313 44-45 0.2315085564983903 2313 46-47 0.3235981026327508 2313 48-49 0.34924957674186174 2313 50-51 0.5141132871666869 2313 52-53 0.5635432747067668 2313 54-55 0.5578270678618225 2313 56-57 0.6634367868768365 2313 58-59 0.9626879202305183 2313 60-61 0.985965135770698 2313 62-63 1.0893141090708696 2313 64-65 1.1102130873029914 2313 66-67 1.1749379271246134 2313 68-69 1.442920544153445 2313 70-71 1.327349098399349 2313 72-73 1.6352783096424766 2313 74-75 1.5242315381039582 2313 76-77 1.730126873225288 2313 78-79 1.6953883160897156 2313 80-81 1.9643560319625983 2313 82-83 2.017053012447086 2313 84-85 2.169021651859289 2313 86-87 2.4516136988483126 2313 88-89 2.5632468023096635 2313 90-91 2.7197242669903297 2313 92-93 2.6984873911594676 2313 94-95 2.6620063834046483 2313 96-97 2.8945585269382015 2313 98-99 2.8132090188231764 2313 100-101 2.7147339574707043 2314 1 -0.4945461039509489 2314 2 -0.49362081434390603 2314 3 -0.6569655365814562 2314 4 -0.4770104426937678 2314 5 -0.42938106462347747 2314 6 -0.47709380838374216 2314 7 -0.3423963926096789 2314 8 -0.2680783893216372 2314 9 -0.3698496083312506 2314 10-11 -0.4446482333439761 2314 12-13 -0.48006158832477297 2314 14-15 -0.5251575058879183 2314 16-17 -0.3989178546805192 2314 18-19 -0.31957713664144904 2314 20-21 -0.2909007356172495 2314 22-23 -0.39926464242666526 2314 24-25 -0.40056735595874216 2314 26-27 -0.2506045471533227 2314 28-29 -0.31168137083652425 2314 30-31 -0.4795021794489429 2314 32-33 -0.3679146473474546 2314 34-35 -0.5120367199381448 2314 36-37 -0.25533067113917696 2314 38-39 -0.17716375819871644 2314 40-41 -0.06005028859415518 2314 42-43 -0.00839544495481448 2314 44-45 0.016570227592829667 2314 46-47 0.1951815977074105 2314 48-49 0.21122706732784735 2314 50-51 0.0712271471922108 2314 52-53 0.2502110637211743 2314 54-55 0.16927518243127082 2314 56-57 0.2403451280384843 2314 58-59 0.406777832011354 2314 60-61 0.25892130521935997 2314 62-63 0.4645305169813412 2314 64-65 0.5452788086608997 2314 66-67 0.7682049653625747 2314 68-69 0.8926099302256354 2314 70-71 1.1817344688237625 2314 72-73 1.2158886035815222 2314 74-75 1.3636542660766544 2314 76-77 1.4192663998362356 2314 78-79 1.6395723950005738 2314 80-81 1.9711448000136436 2314 82-83 2.2020776628172136 2314 84-85 2.3645480311594653 2314 86-87 2.390479069885348 2314 88-89 2.464022765784783 2314 90-91 2.5874485848798976 2314 92-93 2.79789982177104 2314 94-95 2.813612710717866 2314 96-97 2.799092440924966 2314 98-99 3.032984331504114 2314 100-101 2.914586101914706 2315 1 -0.682941144144511 2315 2 -0.5607550066666356 2315 3 -0.5916149427704767 2315 4 -0.6011646098721641 2315 5 -0.514369515909145 2315 6 -0.4347919920940342 2315 7 -0.5751917747852033 2315 8 -0.5915586787840965 2315 9 -0.8083569911427944 2315 10-11 -0.5689149507976907 2315 12-13 -0.5430455411592732 2315 14-15 -0.6799861618881167 2315 16-17 -0.6187079546467587 2315 18-19 -0.6523454640328197 2315 20-21 -0.47546924444180405 2315 22-23 -0.5878739256588048 2315 24-25 -0.5634712717234365 2315 26-27 -0.47243924123549874 2315 28-29 -0.47884196530607426 2315 30-31 -0.3967385688456062 2315 32-33 -0.6127880327375195 2315 34-35 -0.6284713740314558 2315 36-37 -0.4668876917666722 2315 38-39 -0.3965406793998767 2315 40-41 -0.364001711017238 2315 42-43 -0.2740725947570368 2315 44-45 -0.4431412829718866 2315 46-47 -0.47094154071875494 2315 48-49 -0.4026571987132357 2315 50-51 -0.1599954976621234 2315 52-53 0.11070526444513717 2315 54-55 0.07884788890050487 2315 56-57 0.24990590904365462 2315 58-59 0.4629806455902461 2315 60-61 0.4317838734422139 2315 62-63 0.4627002845653152 2315 64-65 0.5056485929761259 2315 66-67 0.601328327465346 2315 68-69 0.9034136293740644 2315 70-71 0.92961835158874 2315 72-73 1.0298876151326084 2315 74-75 1.1951937960235135 2315 76-77 1.264617753079758 2315 78-79 1.3467913945787338 2315 80-81 1.5312497322313234 2315 82-83 1.721945604618579 2315 84-85 1.791372730990787 2315 86-87 1.7780807997302794 2315 88-89 2.047873848183805 2315 90-91 2.1562932803326156 2315 92-93 2.104405785393034 2315 94-95 2.2363053855757222 2315 96-97 2.2273249981538434 2315 98-99 2.190086242010917 2315 100-101 2.314133527586728 2316 1 -0.9560238979017264 2316 2 -0.9648260048164552 2316 3 -0.8233749297884536 2316 4 -0.7324344531528482 2316 5 -0.6365500130747535 2316 6 -0.7169152284060587 2316 7 -0.75658706469995 2316 8 -0.7195021804433495 2316 9 -0.6672427692527663 2316 10-11 -0.8015313772788417 2316 12-13 -0.8352888430811802 2316 14-15 -1.2630851612709861 2316 16-17 -0.981655199496025 2316 18-19 -0.8480762366742951 2316 20-21 -0.6964115631202858 2316 22-23 -0.8189543472367191 2316 24-25 -1.0538403243193244 2316 26-27 -0.6827157305636362 2316 28-29 -0.7822680851110775 2316 30-31 -0.7186562283172577 2316 32-33 -0.6675043616725276 2316 34-35 -0.7415983583595249 2316 36-37 -0.49192610395432723 2316 38-39 -0.5971038771760391 2316 40-41 -0.7213559483023033 2316 42-43 -0.49753482533633786 2316 44-45 -0.626319911860385 2316 46-47 -0.38036134928218246 2316 48-49 -0.16253641523129403 2316 50-51 -0.20511538531213347 2316 52-53 -0.1212901122175083 2316 54-55 -0.09735459204119223 2316 56-57 0.028866044220769993 2316 58-59 0.2258911522812319 2316 60-61 0.038631890275404146 2316 62-63 0.29202071309271105 2316 64-65 0.5165717100851879 2316 66-67 0.548566963218871 2316 68-69 0.6756452523018126 2316 70-71 0.5840647244345938 2316 72-73 0.7092806452584988 2316 74-75 0.9359283902780042 2316 76-77 1.0624296911111095 2316 78-79 1.2901825980520272 2316 80-81 1.625532273401891 2316 82-83 1.9155087052708772 2316 84-85 1.9618140172003464 2316 86-87 2.079029898981819 2316 88-89 2.0765783545502465 2316 90-91 2.3038007259930176 2316 92-93 2.4678279719830627 2316 94-95 2.4889832594920556 2316 96-97 2.392944959099193 2316 98-99 2.7599944931358387 2316 100-101 2.5719627669379417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8601.0 3 3371.0 4 3775.0 5 4330.0 6 5156.0 7 6378.0 8 7932.0 9 10284.0 10 12669.0 11 15140.0 12 17848.0 13 20832.0 14 23007.0 15 26049.0 16 29629.0 17 34261.0 18 34382.0 19 34314.0 20 37354.0 21 40987.0 22 45658.0 23 50237.0 24 56012.0 25 62623.0 26 69762.0 27 78005.0 28 86975.0 29 97028.0 30 109869.0 31 124803.0 32 142937.0 33 166815.0 34 196222.0 35 233935.0 36 267567.0 37 259407.0 38 125481.0 39 7288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.570075750146124 17.41343260747616 13.432254420066139 24.584237222311575 2 16.168287590280798 19.059463946952587 38.99207097818394 25.780177484582673 3 19.637754079990984 22.684053448351417 28.541978365249182 29.13621410640842 4 13.6408916475729 15.209945348007361 34.41545687312975 36.73370613128999 5 15.183143632411975 36.145871568967365 32.74166420784636 15.9293205907743 6 34.85750867532451 33.607197584526695 16.458581488571426 15.076712251577367 7 31.01811788675773 31.164705643022234 19.06967045726617 18.747506012953867 8 27.75832956453564 34.31345546846025 18.615330686568004 19.31288428043611 9 27.468252111374465 16.242187738417275 18.811675491936754 37.477884658271506 10-11 26.571169978849007 25.34760847831568 26.0129073297695 22.06831421306581 12-13 26.8488722394283 22.44559154541939 26.720473783261294 23.985062431891016 14-15 23.56296422205318 24.162265809491377 24.446239191338947 27.828530777116494 16-17 24.04223280263292 27.392489012387138 24.985629213564458 23.579648971415484 18-19 24.057847193575522 25.896412628240224 26.87633017660579 23.169410001578463 20-21 24.393501680761695 25.247791065984764 26.893928131005513 23.46477912224803 22-23 24.2250413463203 24.90835347265079 26.970221847092457 23.896383333936452 24-25 24.094658200675603 25.315293212611962 26.88376713907335 23.706281447639082 26-27 23.791245284052394 25.6663755014286 26.783158846124877 23.759220368394125 28-29 23.712425782704905 25.611285254193255 27.129531675957196 23.54675728714464 30-31 23.694566922664624 25.39559704383003 26.93076318167769 23.979072851827652 32-33 23.624281354143868 25.48584088435961 26.872283836040673 24.017593925455856 34-35 24.23386434232867 25.488314411573704 26.511732534211742 23.766088711885892 36-37 24.326274568102153 25.218354876064097 26.55201552328493 23.903355032548824 38-39 23.788594419234705 25.436803462938617 26.69092955970242 24.083672558124253 40-41 23.859168400022295 25.389606516048087 26.854935002831066 23.896290081098552 42-43 24.08482251126956 25.23865181478648 26.589375710948488 24.087149962995476 44-45 23.798217344564254 25.529869364371883 26.586938627519196 24.084974663544664 46-47 23.954240402176264 25.824381936020895 26.15070813861656 24.070669523186275 48-49 24.189953574170826 25.603244371029565 26.20436674253711 24.002435312262495 50-51 23.988647357213413 25.808038802211314 26.168397013174676 24.0349168274006 52-53 24.26601251679587 25.665963119198203 26.037227328096456 24.030797035909472 54-55 23.84685424991796 25.600592466970983 26.26989475380432 24.28265852930674 56-57 23.90883472798847 25.920638614510022 26.2675195606983 23.90300709680321 58-59 24.109891008179716 25.384653142865076 26.20166645057214 24.303789398383067 60-61 23.875825945745525 25.47509236277391 26.677157784482432 23.97192390699814 62-63 23.757854252682684 25.689866945815997 26.362344095614255 24.18993470588707 64-65 23.694596983635282 25.52704246065271 26.422467067179824 24.355893488532182 66-67 23.724286011805532 25.671797649649324 26.303629169066262 24.30028716947888 68-69 23.616833454451015 25.422080131667567 26.562672338468253 24.398414075413164 70-71 23.6512944436339 25.654145125853628 26.33652131499061 24.358039115521862 72-73 23.86615572126382 25.57242756695695 26.148160346214144 24.413256365565086 74-75 23.588304306748224 25.864192231202505 26.13878979547654 24.40871366657273 76-77 23.888055669038167 25.63393211082413 26.10465949708636 24.373352723051344 78-79 24.015859823410352 25.65125988794477 26.0647495379922 24.268130750652677 80-81 23.764278408679786 26.023518184253806 26.110093106664923 24.102110300401485 82-83 24.00711173485143 25.907179382774792 25.8728176015242 24.212891280849576 84-85 23.96435644958417 25.425216362851295 26.00740530409697 24.603021883467562 86-87 24.088829771943697 25.44850864751541 26.083166252133203 24.37949532840769 88-89 23.97709143262061 25.547449395610705 26.00083671582584 24.474622455942843 90-91 23.939145761502445 25.63701328511605 26.046917571017836 24.37692338236367 92-93 24.11436774359471 25.20449702776466 26.09298955341663 24.588145675224006 94-95 23.938260111016792 25.2131538079386 26.18054115797009 24.66804492307452 96-97 24.150666185010948 25.166093967066562 26.048799130203314 24.634440717719176 98-99 24.234861462356246 25.20197396560522 25.708471025446304 24.854693546592234 100-101 24.744195260369604 24.682377739115182 25.91285569543341 24.6605713050818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 753.0 1 509.0 2 400.5 3 533.5 4 436.5 5 410.0 6 726.0 7 1485.0 8 2297.5 9 2522.0 10 2148.0 11 1784.0 12 1772.5 13 1873.5 14 1971.5 15 2078.0 16 2133.5 17 2217.0 18 2314.0 19 2412.0 20 2624.0 21 3054.0 22 3672.5 23 4241.0 24 5031.0 25 6319.0 26 7650.0 27 9220.0 28 11138.5 29 13042.5 30 15313.5 31 18267.5 32 22104.0 33 25149.5 34 27141.5 35 29876.0 36 34041.5 37 39054.0 38 44495.0 39 48756.0 40 53242.0 41 62159.5 42 71329.0 43 78787.5 44 85812.0 45 92149.0 46 97161.5 47 101188.5 48 106338.5 49 107760.0 50 107015.0 51 106129.5 52 105131.0 53 106596.5 54 107193.0 55 103022.0 56 95210.0 57 88163.5 58 81197.0 59 72248.0 60 62078.5 61 52854.5 62 44370.0 63 35695.0 64 28893.5 65 23622.5 66 18554.0 67 15002.0 68 11624.0 69 8217.0 70 6204.5 71 4832.5 72 4047.0 73 3343.5 74 2601.5 75 2177.5 76 1610.0 77 1039.5 78 643.0 79 413.0 80 255.5 81 154.0 82 92.5 83 52.0 84 32.0 85 22.5 86 16.0 87 13.0 88 10.5 89 4.5 90 2.5 91 4.0 92 4.5 93 4.0 94 2.5 95 1.0 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30321601393549985 2 0.034025271781747045 3 0.040478340567940446 4 0.04360710119154937 5 0.05854693316928199 6 0.054714201405361054 7 0.03030986854121145 8 0.03312575310245948 9 0.039305055334087104 10-11 0.030446751818494343 12-13 0.03197202262250369 14-15 0.027278881687090306 16-17 0.020395608315150672 18-19 0.0248932017115885 20-21 0.03672382781960974 22-23 0.03013387575613345 24-25 0.05029482702451345 26-27 0.04304001332852026 28-29 0.03578519963252707 30-31 0.03570698061693684 32-33 0.03181558459132325 34-35 0.03062274460357234 36-37 0.02984055444767011 38-39 0.01883122800334621 40-41 0.018205475878624425 42-43 0.01873345423385843 44-45 0.009053851054568322 46-47 0.016895307367488187 48-49 0.014548736899781494 50-51 0.006316185508910514 52-53 0.004634476673720718 54-55 0.006101083216037401 56-57 0.005397112075725394 58-59 0.00670728058686163 60-61 0.006140192723832513 62-63 0.004204272087974491 64-65 0.007939230082407643 66-67 0.002757220299555364 68-69 0.00707882091091519 70-71 0.01337545166592815 72-73 0.013883875267264598 74-75 0.010657340874167897 76-77 0.009444946132519439 78-79 0.005788207153676509 80-81 0.006961492387529856 82-83 0.011869735615816354 84-85 0.0041847173340769355 86-87 0.0012710590033411253 88-89 0.019535199143658217 90-91 0.028021962335197423 92-93 0.01351233494321104 94-95 0.021627557810696686 96-97 0.0181272568630342 98-99 0.013434115927620817 100-101 0.10289711500893847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2556923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66988564103814 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1135816841169 34.85316077688553 2 13.79233409978298 13.149579785192907 3 5.142786227245404 7.354680939872833 4 2.451767559993205 4.675019168131329 5 1.45927746796961 3.4781795008327503 6 0.8638236286109255 2.4707024153945647 7 0.6064017819493054 2.023497251863159 8 0.4384965393847532 1.6722463909169734 9 0.31821103236655773 1.3652175170367444 >10 1.597069894892365 13.684760289979502 >50 0.12437979765280974 4.139153892938969 >100 0.08467114276161605 7.733456809232796 >500 0.005461419035206202 1.732597444730355 >1k 0.0016549754652129835 1.4270073802002403 >5k 8.274877326064917E-5 0.24074043679129992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6103 0.2386853260735658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.18541817645662384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3314 0.12960890883299966 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2839 0.11103189263032168 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2721 0.10641697071049852 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.955475389755577E-5 0.0 20-21 0.0 0.0 0.0 1.368832772828904E-4 0.0 22-23 0.0 0.0 0.0 3.9109507795111545E-4 0.0 24-25 0.0 0.0 0.0 8.017449097997867E-4 0.0 26-27 0.0 0.0 0.0 0.0015252708040093502 0.0 28-29 0.0 0.0 0.0 0.0038131770100233754 0.0 30-31 0.0 0.0 0.0 0.011635078569045686 0.0 32-33 0.0 0.0 0.0 0.02925391183074344 0.0 34-35 0.0 0.0 0.0 0.05670878630291174 0.0 36-37 0.0 0.0 0.0 0.10004212093989534 0.0 38-39 0.0 0.0 0.0 0.16631318189871186 0.0 40-41 0.0 0.0 0.0 0.239995494584702 0.0 42-43 0.0 0.0 0.0 0.31346270497781903 0.0 44-45 0.0 0.0 0.0 0.4068171000847503 0.0 46-47 0.0 0.0 0.0 0.5212124103854516 0.0 48-49 0.0 0.0 0.0 0.6425692130736826 0.0 50-51 0.0 0.0 0.0 0.7712590484734972 0.0 52-53 0.0 0.0 0.0 0.9017479212318869 0.0 54-55 0.0 0.0 0.0 1.0424248207709033 0.0 56-57 0.0 0.0 0.0 1.2139982314680575 0.0 58-59 0.0 0.0 0.0 1.395603230914658 0.0 60-61 0.0 3.910950779511154E-5 0.0 1.595452815747678 0.0 62-63 0.0 3.910950779511154E-5 0.0 1.7929558301129913 0.0 64-65 0.0 3.910950779511154E-5 0.0 1.9971270155573713 0.0 66-67 0.0 3.910950779511154E-5 0.0 2.236790079325815 0.0 68-69 0.0 3.910950779511154E-5 0.0 2.4999188477713252 0.0 70-71 0.0 3.910950779511154E-5 0.0 2.7838734291177323 0.0 72-73 0.0 5.866426169266731E-5 0.0 3.0682973245576814 0.0 74-75 0.0 7.821901559022309E-5 0.0 3.362009728098969 0.0 76-77 0.0 7.821901559022309E-5 0.0 3.6761372947093047 0.0 78-79 0.0 7.821901559022309E-5 0.0 4.026890915369763 0.0 80-81 0.0 1.1732852338533464E-4 0.0 4.407543754739583 0.0 82-83 0.0 1.1732852338533464E-4 0.0 4.791012478670652 0.0 84-85 3.910950779511154E-5 1.368832772828904E-4 0.0 5.175322057019316 0.0 86-87 3.910950779511154E-5 1.7599278507800193E-4 0.0 5.5743368102989415 0.0 88-89 3.910950779511154E-5 1.9554753897555772E-4 0.0 6.000513116742272 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4380 0.0 41.13696 1 GTATCAA 6895 0.0 37.8153 1 GTACATG 9480 0.0 29.213411 1 ATCAACG 8980 0.0 28.739418 3 TATCAAC 9120 0.0 28.402472 2 TCAACGC 9090 0.0 28.391636 4 TACATGG 9795 0.0 27.852356 2 CAACGCA 9285 0.0 27.846556 5 AACGCAG 9610 0.0 26.95005 6 ACATGGG 10275 0.0 25.764864 3 ACGCAGA 11170 0.0 23.136877 7 CGCAGAG 11515 0.0 22.402416 8 GTATAGG 440 0.0 21.66665 1 CATGGGG 6745 0.0 21.49178 4 GAGTACT 5695 0.0 20.43487 12-13 GCAGAGT 12495 0.0 19.960207 9 GGACCGA 715 0.0 19.274292 6 AGTACTT 6035 0.0 19.244255 12-13 ATTCGCG 500 0.0 18.9958 80-81 GTACTTT 6085 0.0 18.93093 14-15 >>END_MODULE