FastQCFastQC Report
Thu 26 May 2016
SRR938557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556923
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.2303941104210021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.20469916379961384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.13637485368155397No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29370.11486462439424261No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC26860.10504813793766961No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG26090.10203670583744603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.042.725051
GTATCAA72250.036.6656231
ATCAACG91450.028.6963773
TATCAAC92250.028.6648852
TCAACGC93400.028.1481554
GTACATG93600.027.7433261
CAACGCA95650.027.486025
TACATGG96500.026.6631642
AACGCAG99500.026.5875876
ACATGGG100450.025.0367183
ACGCAGA115000.023.0453457
CGCAGAG117900.022.3576438
GTATAGG4050.022.3120941
CATGGGG65550.022.1932264
TAAGGTG9150.021.822275
GCAGAGT127050.020.3736449
GAGTACT61800.020.05852112-13
TTACGTA1707.9056554E-519.575864
GTACTTT63800.019.39250414-15
GTAAGGT7500.019.0165484
Produced by FastQC (version 0.11.5)