##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2556923 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795114283848203 31.0 31.0 34.0 30.0 34.0 2 32.02184578886419 31.0 31.0 34.0 30.0 34.0 3 32.09691296922121 33.0 31.0 34.0 30.0 34.0 4 35.76759487868817 37.0 35.0 37.0 35.0 37.0 5 35.63034436312709 37.0 35.0 37.0 33.0 37.0 6 35.66632511029859 37.0 35.0 37.0 33.0 37.0 7 35.595102003462756 37.0 35.0 37.0 33.0 37.0 8 35.583151702260885 37.0 35.0 37.0 33.0 37.0 9 37.15328580485216 39.0 37.0 39.0 33.0 39.0 10-11 37.077562171406804 39.0 37.0 39.0 33.0 39.0 12-13 36.95982886461579 39.0 37.0 39.0 33.0 39.0 14-15 38.023286974226444 40.0 37.0 41.0 33.0 41.0 16-17 37.93637039519767 40.0 37.0 41.0 33.0 41.0 18-19 37.90337096580539 40.0 37.0 41.0 33.0 41.0 20-21 37.88378355546882 40.0 37.0 41.0 32.5 41.0 22-23 37.91074213028707 40.0 37.0 41.0 32.5 41.0 24-25 37.83273274165863 40.0 37.0 41.0 32.0 41.0 26-27 37.655325561231216 40.0 37.0 41.0 32.0 41.0 28-29 37.515118562428356 39.5 36.5 41.0 32.0 41.0 30-31 37.28639755675083 39.0 36.0 41.0 31.0 41.0 32-33 37.09603026763027 39.0 36.0 41.0 31.0 41.0 34-35 36.92799763622135 39.0 36.0 40.5 30.5 41.0 36-37 36.958162604036175 39.0 36.0 41.0 31.0 41.0 38-39 36.81968835197618 39.0 35.0 41.0 30.0 41.0 40-41 36.64381367761172 39.0 35.0 40.5 30.0 41.0 42-43 36.419958285798984 39.0 35.0 40.0 30.0 41.0 44-45 36.14727037145819 38.0 35.0 40.0 30.0 41.0 46-47 35.7930753096593 38.0 35.0 40.0 28.5 41.0 48-49 35.4116017572684 38.0 34.0 40.0 27.5 41.0 50-51 35.03293411651427 38.0 34.0 40.0 26.5 41.0 52-53 34.49567839938864 37.5 33.0 40.0 25.5 41.0 54-55 34.13601250409184 37.0 33.0 40.0 24.0 41.0 56-57 33.73958856015609 37.0 32.5 39.5 23.0 41.0 58-59 33.264139553674475 36.0 31.5 39.0 22.5 40.5 60-61 32.828913698222436 36.0 31.0 39.0 22.0 40.0 62-63 32.318941360377295 35.0 31.0 39.0 20.5 40.0 64-65 31.796116073890374 35.0 30.0 38.0 18.5 40.0 66-67 31.265973594042528 34.5 29.5 38.0 17.0 40.0 68-69 30.686780947255745 34.0 29.0 37.0 13.0 39.0 70-71 30.101019076444615 34.0 29.0 36.5 8.0 39.0 72-73 29.501385258766106 34.0 28.0 36.0 7.0 39.0 74-75 28.87038366036052 33.0 27.0 35.0 4.5 38.0 76-77 27.614312984786793 31.5 26.0 34.0 2.0 36.0 78-79 28.240452293635748 33.0 26.5 35.0 2.0 36.0 80-81 27.940261009033122 33.0 26.0 35.0 2.0 36.0 82-83 27.449546779468918 33.0 26.0 35.0 2.0 36.0 84-85 26.866392926185107 32.0 25.0 35.0 2.0 35.0 86-87 26.208260475579436 32.0 24.0 34.5 2.0 35.0 88-89 25.46190968597803 31.0 21.0 34.0 2.0 35.0 90-91 24.921370725673007 31.0 19.5 34.0 2.0 35.0 92-93 24.1242290049407 31.0 15.0 34.0 2.0 35.0 94-95 23.313236847570302 30.5 4.5 34.0 2.0 35.0 96-97 22.309469428684398 30.0 2.0 34.0 2.0 35.0 98-99 21.11099982283393 29.0 2.0 34.0 2.0 35.0 100-101 17.986110258306567 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3978774730856536 1101 2 0.442514906623515 1101 3 0.40311821056346986 1101 4 0.28616380266534946 1101 5 0.2836426672334653 1101 6 0.21440893501460323 1101 7 0.29229578593928096 1101 8 0.35518794395952824 1101 9 0.4117752359695288 1101 10-11 0.4097647699322451 1101 12-13 0.3467553932673937 1101 14-15 0.5363350331008903 1101 16-17 0.5621216340061679 1101 18-19 0.6662680975409287 1101 20-21 0.6920250515698498 1101 22-23 0.5987602259878955 1101 24-25 0.6090883020889919 1101 26-27 0.48639004541117714 1101 28-29 0.4842359300371726 1101 30-31 0.5147907649397467 1101 32-33 0.45736878634907185 1101 34-35 0.31187767435022096 1101 36-37 0.5728342620832549 1101 38-39 0.6551401892654027 1101 40-41 0.8540544979107167 1101 42-43 0.7144935742434058 1101 44-45 0.8317562502380085 1101 46-47 0.8428461322974101 1101 48-49 0.9414148305870924 1101 50-51 1.2174701556773826 1101 52-53 1.137330397730679 1101 54-55 1.254713197141058 1101 56-57 1.310940845947428 1101 58-59 1.4672462321535207 1101 60-61 1.4917716782590063 1101 62-63 1.5197877604628403 1101 64-65 1.5726581617364381 1101 66-67 1.561875377903121 1101 68-69 1.8311391677507203 1101 70-71 1.9144476836961992 1101 72-73 1.9005089794269736 1101 74-75 1.8842554462401715 1101 76-77 1.737285100472569 1101 78-79 2.06653431929578 1101 80-81 2.2808353824921923 1101 82-83 2.4436143803852524 1101 84-85 2.833465427799016 1101 86-87 2.7836746439006674 1101 88-89 3.027532274463031 1101 90-91 3.328657410003583 1101 92-93 3.467169787515264 1101 94-95 3.6035491354638367 1101 96-97 3.9605164439654708 1101 98-99 4.237826864684923 1101 100-101 4.517957991326494 1102 1 0.2285882397842265 1102 2 0.24245755970069638 1102 3 0.23392505197688251 1102 4 0.21487599186550455 1102 5 0.1564565019924018 1102 6 0.11710721944789526 1102 7 0.16494353805162376 1102 8 0.24242452980675466 1102 9 0.3065972556983567 1102 10-11 0.3084286611071434 1102 12-13 0.30918738689220504 1102 14-15 0.3619547708690476 1102 16-17 0.33952787737310786 1102 18-19 0.39891860528462075 1102 20-21 0.5275408573499689 1102 22-23 0.5071803555025696 1102 24-25 0.3674747309769444 1102 26-27 0.45033949819492136 1102 28-29 0.31029843073538643 1102 30-31 0.32856147406796765 1102 32-33 0.233636283677221 1102 34-35 0.46436072163164255 1102 36-37 0.41021793906367776 1102 38-39 0.4313894398453968 1102 40-41 0.5089388544894149 1102 42-43 0.564079857157374 1102 44-45 0.5669007905502923 1102 46-47 0.6560005481966513 1102 48-49 0.6337895517656165 1102 50-51 0.6825196267078724 1102 52-53 0.8358714026424039 1102 54-55 0.767321513934391 1102 56-57 1.0304034233564607 1102 58-59 1.0654571198764344 1102 60-61 1.0022127282290185 1102 62-63 1.1452375055669322 1102 64-65 1.3131912646890562 1102 66-67 1.2074159098889226 1102 68-69 1.2953306692346658 1102 70-71 1.4009683639647612 1102 72-73 1.5777720416781769 1102 74-75 1.6396704480462283 1102 76-77 1.5844127271664448 1102 78-79 1.9747334397280376 1102 80-81 2.1373552435190035 1102 82-83 2.153565374833029 1102 84-85 2.3826000106001537 1102 86-87 2.4291516846504955 1102 88-89 2.638557220374455 1102 90-91 2.8586604635410318 1102 92-93 2.9535225232241515 1102 94-95 2.9885519841908454 1102 96-97 3.388736529538992 1102 98-99 3.7134439957747745 1102 100-101 4.426590378932115 1103 1 0.2640718496386576 1103 2 0.2634223475055322 1103 3 0.2619943722472797 1103 4 0.1863937804446465 1103 5 0.12544848309952528 1103 6 0.18436658431976838 1103 7 0.15378163111224552 1103 8 0.2195367627100211 1103 9 0.3074402565898211 1103 10-11 0.3594161028967733 1103 12-13 0.4168378374334907 1103 14-15 0.52932515127209 1103 16-17 0.5066853330405579 1103 18-19 0.49397560946998453 1103 20-21 0.5340980338987436 1103 22-23 0.4213060966563873 1103 24-25 0.4094238328233146 1103 26-27 0.33802227708372357 1103 28-29 0.3818848096586933 1103 30-31 0.43314328775713307 1103 32-33 0.49226102242505476 1103 34-35 0.4282438404189719 1103 36-37 0.6509886857789269 1103 38-39 0.6965607044674371 1103 40-41 0.584720267855225 1103 42-43 0.7291405500263579 1103 44-45 0.7999750048056526 1103 46-47 0.8899135918292771 1103 48-49 1.005908056141088 1103 50-51 0.9904891182056161 1103 52-53 1.1423791778123515 1103 54-55 1.1495611768334442 1103 56-57 1.1185216018212998 1103 58-59 1.3865038646220142 1103 60-61 1.4057066110140894 1103 62-63 1.490101200009029 1103 64-65 1.3722532491298374 1103 66-67 1.572702095289479 1103 68-69 1.5264188877069813 1103 70-71 1.4565583172544514 1103 72-73 1.5059001359210242 1103 74-75 1.5813042189211082 1103 76-77 1.5725466331273807 1103 78-79 1.9737761215962983 1103 80-81 2.1751610554501006 1103 82-83 2.3520828541875822 1103 84-85 2.5125109922621753 1103 86-87 2.659237053357323 1103 88-89 2.91068071281585 1103 90-91 3.0036761431090966 1103 92-93 3.0816541321896054 1103 94-95 3.1871854671424913 1103 96-97 3.5527537225679175 1103 98-99 4.15135420206769 1103 100-101 4.512970544819918 1104 1 0.3150010172901183 1104 2 0.2909159838676274 1104 3 0.24573712654199653 1104 4 0.22551353246256411 1104 5 0.22648808983775837 1104 6 0.19314049791032062 1104 7 0.2150391913861398 1104 8 0.21099239918434165 1104 9 0.23676587250665193 1104 10-11 0.27855590228702454 1104 12-13 0.24764447636144382 1104 14-15 0.4615343410963959 1104 16-17 0.55382471413958 1104 18-19 0.5424920359025904 1104 20-21 0.531271337396241 1104 22-23 0.4540082239903569 1104 24-25 0.45932640157355564 1104 26-27 0.44310766120599965 1104 28-29 0.377289132341474 1104 30-31 0.38613143447193465 1104 32-33 0.4548290585137238 1104 34-35 0.4562390861820731 1104 36-37 0.4818000007267145 1104 38-39 0.5983467740092081 1104 40-41 0.6608496966836555 1104 42-43 0.5794911664958065 1104 44-45 0.6943844405035762 1104 46-47 0.8661620466172693 1104 48-49 0.9008603352331619 1104 50-51 0.9578944543094181 1104 52-53 1.2356333815541873 1104 54-55 1.4143718320373466 1104 56-57 1.424713162200618 1104 58-59 1.4102851663239093 1104 60-61 1.3159311959960789 1104 62-63 1.4554946573220633 1104 64-65 1.511373851333186 1104 66-67 1.7423316014847785 1104 68-69 1.8012278085326159 1104 70-71 1.8639482990094756 1104 72-73 2.027469510201552 1104 74-75 2.126016490667574 1104 76-77 2.088857270477387 1104 78-79 2.354016707067988 1104 80-81 2.728951097058175 1104 82-83 2.825972724568313 1104 84-85 2.8220892662837365 1104 86-87 2.9856472134132304 1104 88-89 2.908457562946861 1104 90-91 3.2903706561823967 1104 92-93 3.594911498621226 1104 94-95 3.594825063338419 1104 96-97 4.006120966963916 1104 98-99 4.393014223118282 1104 100-101 4.829702449048231 1105 1 0.26978000066023 1105 2 0.2588748005049055 1105 3 0.17886237867437416 1105 4 0.18941958733621078 1105 5 0.18120165942146826 1105 6 0.1929604894563539 1105 7 0.23398668838466108 1105 8 0.21048451818921166 1105 9 0.31242230065675614 1105 10-11 0.24542398019853806 1105 12-13 0.34861587912553205 1105 14-15 0.41867987310977384 1105 16-17 0.33600243070019786 1105 18-19 0.43757418886798405 1105 20-21 0.38476551042614915 1105 22-23 0.26860972223929025 1105 24-25 0.2419027886431664 1105 26-27 0.3282043273529993 1105 28-29 0.45597572958702415 1105 30-31 0.6311398161155708 1105 32-33 0.5487661487735025 1105 34-35 0.593919355809021 1105 36-37 0.6448771327420246 1105 38-39 0.6290481801645242 1105 40-41 0.5773559093861493 1105 42-43 0.6609821990210847 1105 44-45 0.606514519112622 1105 46-47 0.7900056932028079 1105 48-49 0.7338071861299085 1105 50-51 0.8123364843547662 1105 52-53 0.8749381471172768 1105 54-55 0.8198617684397078 1105 56-57 0.8957292295623489 1105 58-59 1.0938832620916301 1105 60-61 1.0622100033757746 1105 62-63 1.265415614324148 1105 64-65 1.2778165789112812 1105 66-67 1.307619703408136 1105 68-69 1.387690610267768 1105 70-71 1.4414482065944192 1105 72-73 1.4945265166288202 1105 74-75 1.7992997002183095 1105 76-77 1.7109382003496307 1105 78-79 1.9830106848208722 1105 80-81 2.263698836103778 1105 82-83 2.2812887599464027 1105 84-85 2.5331534376903626 1105 86-87 2.694613884526536 1105 88-89 2.813327193431295 1105 90-91 2.9516655437815835 1105 92-93 3.173582023683384 1105 94-95 3.0821813981091424 1105 96-97 3.3958641013289785 1105 98-99 3.989734131571126 1105 100-101 4.5350643705099 1106 1 0.1380208428921108 1106 2 0.15324863408008582 1106 3 0.04647340899495589 1106 4 0.1020830255890317 1106 5 -0.01975132165387805 1106 6 0.1114021466848314 1106 7 0.13268942810612572 1106 8 0.03462266634552691 1106 9 0.12886345257656018 1106 10-11 0.22225075591206434 1106 12-13 0.1544864684001439 1106 14-15 0.13553889595445412 1106 16-17 0.08400145751311783 1106 18-19 0.23385826687076872 1106 20-21 0.42373026522535184 1106 22-23 0.21031191462232357 1106 24-25 0.12856598613685577 1106 26-27 0.2047930719115385 1106 28-29 0.23852488021326224 1106 30-31 0.4551368870381651 1106 32-33 0.4587158922889003 1106 34-35 0.24971913684192515 1106 36-37 0.4462598958161692 1106 38-39 0.5409798248744764 1106 40-41 0.46482298541733513 1106 42-43 0.6338308980891512 1106 44-45 0.23992274999776697 1106 46-47 0.04576571250159844 1106 48-49 0.46179433993359 1106 50-51 0.4310140791806347 1106 52-53 0.6978215088378832 1106 54-55 0.766786990527315 1106 56-57 0.703286242924797 1106 58-59 1.1449746320824161 1106 60-61 0.8718756416087103 1106 62-63 1.1081221116643007 1106 64-65 0.7306461092390855 1106 66-67 0.8660990319851862 1106 68-69 0.9233579325457448 1106 70-71 1.19187996319868 1106 72-73 1.6259435680527794 1106 74-75 1.838687924622942 1106 76-77 1.6322437492130248 1106 78-79 1.8854066504719356 1106 80-81 1.7733425237024747 1106 82-83 2.1492587085130843 1106 84-85 2.029319213828469 1106 86-87 2.240608789885833 1106 88-89 2.6433479212375595 1106 90-91 2.7291539947186614 1106 92-93 2.3797749256666627 1106 94-95 2.956101491139229 1106 96-97 3.3208957069116067 1106 98-99 3.6224330905178874 1106 100-101 3.931210194409669 1107 1 0.20146136222999544 1107 2 0.19660565914794148 1107 3 0.11355460254660699 1107 4 0.1582964523799859 1107 5 0.00901072206799114 1107 6 0.07983015652980896 1107 7 0.17955918514589797 1107 8 0.18153907148539616 1107 9 0.22312564658572143 1107 10-11 0.21814651526944573 1107 12-13 0.2694798642786935 1107 14-15 0.38585363237474724 1107 16-17 0.43845751690103896 1107 18-19 0.3949663214178045 1107 20-21 0.4251360605007264 1107 22-23 0.35385077909607077 1107 24-25 0.2897196275808156 1107 26-27 0.3983583992052786 1107 28-29 0.46502547007781203 1107 30-31 0.451740289554472 1107 32-33 0.3635564584691977 1107 34-35 0.5673598908701294 1107 36-37 0.5584900531477572 1107 38-39 0.6403417727293501 1107 40-41 0.6615816374740788 1107 42-43 0.7820726114749093 1107 44-45 0.8025301501112736 1107 46-47 0.8535866752699661 1107 48-49 0.8954143625357247 1107 50-51 0.8378765363382854 1107 52-53 1.0268445637010402 1107 54-55 0.9623353478371115 1107 56-57 0.8698093482324794 1107 58-59 0.9125845887987722 1107 60-61 1.0685854185333383 1107 62-63 1.1710382322223651 1107 64-65 1.2234634078671824 1107 66-67 1.448085496712359 1107 68-69 1.5200934025444042 1107 70-71 1.5773225574129697 1107 72-73 1.4228776325102608 1107 74-75 1.4867140344060523 1107 76-77 1.6043098923627568 1107 78-79 1.8314171169245377 1107 80-81 2.0982182172024864 1107 82-83 2.121696721169503 1107 84-85 2.345254718981355 1107 86-87 2.6622932660826777 1107 88-89 2.867071820683929 1107 90-91 2.9883693111985927 1107 92-93 3.273574345412726 1107 94-95 3.337723230797991 1107 96-97 3.7436350340101505 1107 98-99 3.738532155478321 1107 100-101 3.714844543254653 1108 1 0.11861271157732034 1108 2 0.16749355741991678 1108 3 0.13093163917123718 1108 4 0.1506425269657825 1108 5 0.10831606984228159 1108 6 0.06802607648425862 1108 7 0.09846368235311331 1108 8 0.10528597685959795 1108 9 0.01813738123000519 1108 10-11 0.07637593692844291 1108 12-13 0.14759589943760432 1108 14-15 0.2850061793838137 1108 16-17 0.26031459116406097 1108 18-19 0.30454412250917073 1108 20-21 0.34918574789249845 1108 22-23 0.32147982546527487 1108 24-25 0.2558917853540308 1108 26-27 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0.5709942192454598 2316 72-73 0.6329676923694088 2316 74-75 0.8390630246251867 2316 76-77 0.7742991995430444 2316 78-79 0.9178735232668451 2316 80-81 1.1124692775008214 2316 82-83 1.5529028720170537 2316 84-85 1.5680248453102976 2316 86-87 1.7232751368988275 2316 88-89 2.060567690626417 2316 90-91 2.0984862762462875 2316 92-93 1.984560743984023 2316 94-95 2.5255719512465475 2316 96-97 2.7498238153958887 2316 98-99 3.0255783068276507 2316 100-101 3.2530561832049507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 15.0 4 49.0 5 100.0 6 223.0 7 535.0 8 1160.0 9 1932.0 10 3069.0 11 4828.0 12 6809.0 13 9719.0 14 12541.0 15 16480.0 16 20207.0 17 24983.0 18 25369.0 19 23776.0 20 25163.0 21 27227.0 22 29777.0 23 33435.0 24 38361.0 25 44512.0 26 53222.0 27 63256.0 28 74701.0 29 88299.0 30 103807.0 31 123039.0 32 145445.0 33 175259.0 34 217809.0 35 274219.0 36 339556.0 37 363269.0 38 177062.0 39 7703.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25475308369174 18.246491187245276 14.083707774864983 24.415047954198002 2 16.63640201628696 19.71690129107003 38.021204427442505 25.625492265200506 3 19.730816658642684 22.901077429515755 29.028882025594083 28.33922388624747 4 14.151345191075368 15.941074486795262 34.17670379592972 35.73087652619966 5 15.132091736647252 36.1965769638964 32.99324028861583 15.678091010840514 6 34.912275418540176 33.98780487327932 16.16094813961938 14.938971568561119 7 30.843361337044566 31.26269347962375 19.14461248930844 18.749332694023245 8 27.629850410043634 34.71903534052453 18.475761686996442 19.175352562435396 9 27.10981128489204 16.245776662026977 18.82289767818585 37.82151437489514 10-11 26.52000079783396 25.446640356397122 26.071942721779262 21.961416123989654 12-13 26.712341357170317 22.500169148621215 26.85315513998662 23.93433435422185 14-15 23.33445316890653 24.257535326640653 24.504648751644066 27.903362752808746 16-17 23.86204042906259 27.524528505551398 25.118687578781213 23.4947434866048 18-19 23.8931324877597 25.968263416614423 26.990957490702694 23.14764660492318 20-21 24.216841883779843 25.274382529313556 27.04281669803901 23.465958888867597 22-23 24.102387909217445 24.922142747356883 27.112803162238365 23.862666181187308 24-25 24.07674223873703 25.297616603249956 27.037909622483813 23.5877315355292 26-27 23.66954850728974 25.742209066073595 26.91855702099806 23.669685405638607 28-29 23.635029291065862 25.637651192468446 27.25389071160923 23.47342880485646 30-31 23.591852394460062 25.419674350772393 27.056289923474424 23.93218333129312 32-33 23.56186595758078 25.463088802212987 26.986706986214205 23.98833825399203 34-35 24.146591706065575 25.48531356601473 26.657560619946548 23.71053410797315 36-37 24.25106415349076 25.16328231928066 26.749143328818004 23.83651019841058 38-39 23.674207051012637 25.45881531081277 26.94376912565715 23.923208512517448 40-41 23.785171842148852 25.364618096290588 27.02573051222636 23.824479549334203 42-43 24.025816055422816 25.194688164531886 26.74073375858159 24.038762021463707 44-45 23.75800960047183 25.433467345704397 26.72212154509439 24.08640150872938 46-47 23.886795876759585 25.758441500539124 26.305114628101535 24.049647994599756 48-49 24.0667591476161 25.606715571802514 26.332666255495376 23.99385902508601 50-51 23.89763008115614 25.690409918484054 26.34301072030718 24.068949280052625 52-53 24.22652148695913 25.690820568315903 26.11762262688395 23.965035317841014 54-55 23.856545543217376 25.57329649739159 26.294084726055495 24.276073233335538 56-57 23.86290083823408 25.87758802279146 26.348173175336136 23.911337963638328 58-59 24.051271782529234 25.392669235639868 26.257869321837223 24.29818965999367 60-61 23.80392370047905 25.500298601092013 26.675402427057836 24.020375271371098 62-63 23.654427606932238 25.70851762059319 26.372186413122336 24.26486835935224 64-65 23.51185389626516 25.54036629182811 26.41094393534729 24.536835876559444 66-67 23.648485471867797 25.64162682541505 26.34112115814407 24.368766544573088 68-69 23.487857847667446 25.458608583220666 26.48914572577387 24.564387843338018 70-71 23.45502778143886 25.701223697389402 26.320131658247043 24.523616862924694 72-73 23.672613528056967 25.611408712737926 26.084203552473028 24.631774206732075 74-75 23.43881689045779 25.86235486950526 25.934805232695705 24.764023007341248 76-77 23.545175196906595 25.635910819371567 26.241071788239225 24.577842195482617 78-79 23.57247363334758 25.635930374125465 26.179630751493104 24.61196524103385 80-81 23.3195765612763 26.032329880940857 26.214306456296583 24.43378710148626 82-83 23.526754592235317 25.823307348065377 26.032467081687866 24.61747097801144 84-85 23.36849305853236 25.49473797015654 26.111131535883803 25.0256374354273 86-87 23.4409817084391 25.425072143321014 26.249570034215402 24.88437611402448 88-89 23.252712930904003 25.607244025138048 26.03349888400932 25.106544159948623 90-91 23.170485839990317 25.68425103587291 26.198212954233863 24.94705016990291 92-93 23.233820260884492 25.289811910202797 26.227445005058826 25.24892282385388 94-95 23.00837764766479 25.259951120937156 26.305524257085565 25.426146974312484 96-97 23.0787160974343 25.225652082600845 26.27576192165349 25.41986989831137 98-99 23.085931801622497 25.280776933838055 25.98263616072913 25.650655103810323 100-101 23.13541336473594 24.927368116201627 26.182227270869557 25.754991248192884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 724.0 1 487.0 2 376.0 3 524.0 4 459.0 5 424.5 6 750.0 7 1505.0 8 2206.5 9 2437.0 10 2126.5 11 1724.5 12 1723.0 13 1845.0 14 1985.0 15 2078.5 16 2107.0 17 2202.5 18 2338.0 19 2449.5 20 2624.5 21 2985.5 22 3487.5 23 4105.0 24 4970.0 25 6221.5 26 7467.5 27 8960.5 28 10724.0 29 12760.5 30 15308.5 31 18338.0 32 22410.5 33 25499.5 34 27292.0 35 30002.0 36 34146.5 37 39217.5 38 45023.5 39 49275.5 40 53561.5 41 63030.5 42 72580.0 43 80048.5 44 86740.0 45 93150.0 46 98352.5 47 102113.0 48 107811.0 49 109402.0 50 108777.0 51 107714.5 52 105241.0 53 106780.5 54 108976.5 55 104726.0 56 95649.0 57 88293.5 58 81535.0 59 72343.0 60 61367.0 61 51180.0 62 42868.0 63 34146.5 64 27098.0 65 21932.5 66 16653.5 67 13501.5 68 10378.0 69 6938.0 70 5167.0 71 4150.5 72 3482.5 73 2902.5 74 2353.0 75 1898.5 76 1282.0 77 744.0 78 457.0 79 290.5 80 179.0 81 93.5 82 46.0 83 24.5 84 9.5 85 9.0 86 6.0 87 3.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.5643803118044617E-4 2 0.044428400855246714 3 1.5643803118044617E-4 4 0.0 5 0.09871239767486155 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010168472026729001 26-27 0.0110093264443239 28-29 0.0 30-31 0.0 32-33 6.257521247217847E-4 34-35 0.014861612962142388 36-37 0.0030700963619162565 38-39 0.012182611678177246 40-41 0.006746390094656742 42-43 0.005553550106905839 44-45 0.0027181107917602526 46-47 7.821901559022309E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7599278507800196E-4 68-69 1.5643803118044617E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.955475389755577E-5 82-83 9.972924487753445E-4 84-85 0.004497593396437828 86-87 0.0013101685111362369 88-89 7.821901559022309E-5 90-91 8.408544175948982E-4 92-93 2.346570467706693E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.799639253900097E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2556923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.702956790064036 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6241997971409 31.571105518644387 2 14.807993293740203 13.239221687152527 3 5.709120748803671 7.656437344290855 4 2.722952055743803 4.868960323573251 5 1.584845387177982 3.5423637430974813 6 0.9939655419580423 2.6659919203777767 7 0.6547277393984127 2.0487786090508484 8 0.4686753741466738 1.6760939999236681 9 0.33902991996417264 1.3640075876427553 >10 1.8464899067596137 14.802808465430875 >50 0.141414555315178 4.404112868423222 >100 0.09861061638348532 8.55988716310185 >500 0.005784111965648528 1.7171976787008936 >1k 0.0020156753819675164 1.447191144624672 >5k 1.7527612017108837E-4 0.43584194596506426 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.2303941104210021 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5234 0.20469916379961384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3487 0.13637485368155397 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2937 0.11486462439424261 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2686 0.10504813793766961 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2609 0.10203670583744603 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.955475389755577E-5 0.0 20-21 0.0 0.0 0.0 1.368832772828904E-4 0.0 22-23 0.0 0.0 0.0 3.9109507795111545E-4 0.0 24-25 0.0 0.0 0.0 8.017449097997867E-4 0.0 26-27 0.0 0.0 0.0 0.0015252708040093502 0.0 28-29 0.0 0.0 0.0 0.003871841271716043 0.0 30-31 0.0 0.0 0.0 0.011811071354123686 0.0 32-33 0.0 0.0 0.0 0.02964500690869455 0.0 34-35 0.0 0.0 0.0 0.05741275744322375 0.0 36-37 0.0 0.0 0.0 0.10176293928288024 0.0 38-39 0.0 0.0 0.0 0.16903129269047212 0.0 40-41 0.0 0.0 0.0 0.2427722696381549 0.0 42-43 0.0 0.0 0.0 0.3165132465858378 0.0 44-45 0.0 0.0 0.0 0.41045428430969566 0.0 46-47 0.0 0.0 0.0 0.5252211349344506 0.0 48-49 0.0 0.0 0.0 0.647047251716223 0.0 50-51 0.0 0.0 0.0 0.7761086274400911 0.0 52-53 0.0 0.0 0.0 0.9063823979056076 0.0 54-55 0.0 0.0 0.0 1.0478023780927308 0.0 56-57 0.0 0.0 0.0 1.2193366792820903 0.0 58-59 0.0 0.0 0.0 1.401410992822232 0.0 60-61 0.0 0.0 0.0 1.6013583514247398 0.0 62-63 0.0 0.0 0.0 1.799076468082926 0.0 64-65 0.0 0.0 0.0 2.0038929604059255 0.0 66-67 0.0 0.0 0.0 2.2440644477757052 0.0 68-69 0.0 0.0 0.0 2.509168246364869 0.0 70-71 0.0 0.0 0.0 2.7962515883348855 0.0 72-73 0.0 0.0 0.0 3.0831589375198236 0.0 74-75 0.0 0.0 0.0 3.3819360223205783 0.0 76-77 0.0 0.0 0.0 3.7014215914988444 0.0 78-79 0.0 0.0 0.0 4.059821120933247 0.0 80-81 0.0 0.0 0.0 4.451620170024674 0.0 82-83 0.0 0.0 0.0 4.846684863016994 0.0 84-85 0.0 0.0 0.0 5.240967365853411 0.0 86-87 0.0 0.0 0.0 5.651754080979365 0.0 88-89 0.0 0.0 0.0 6.094063059388178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 42.72505 1 GTATCAA 7225 0.0 36.665623 1 ATCAACG 9145 0.0 28.696377 3 TATCAAC 9225 0.0 28.664885 2 TCAACGC 9340 0.0 28.148155 4 GTACATG 9360 0.0 27.743326 1 CAACGCA 9565 0.0 27.48602 5 TACATGG 9650 0.0 26.663164 2 AACGCAG 9950 0.0 26.587587 6 ACATGGG 10045 0.0 25.036718 3 ACGCAGA 11500 0.0 23.045345 7 CGCAGAG 11790 0.0 22.357643 8 GTATAGG 405 0.0 22.312094 1 CATGGGG 6555 0.0 22.193226 4 TAAGGTG 915 0.0 21.82227 5 GCAGAGT 12705 0.0 20.373644 9 GAGTACT 6180 0.0 20.058521 12-13 TTACGTA 170 7.9056554E-5 19.57586 4 GTACTTT 6380 0.0 19.392504 14-15 GTAAGGT 750 0.0 19.016548 4 >>END_MODULE