FastQCFastQC Report
Thu 26 May 2016
SRR938556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543537
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57850.22743919195985746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47090.18513589540863765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.12002970666438112No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG27780.10921799053837235No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG26640.10473604276249962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.040.530681
GTATCAA72050.035.6977271
GTACATG94400.029.6723461
TACATGG97450.028.3877642
ATCAACG91750.027.3543533
TATCAAC92050.027.2130282
TCAACGC92750.027.0081794
ACATGGG100450.026.7833613
CAACGCA94950.026.3323355
AACGCAG98850.025.4291786
CTATACG1201.7034801E-423.7666854
CATGGGG65900.022.7929364
ACGCAGA111800.022.2680917
CGCAGAG116200.021.3840078
GAGTACT55850.021.3501712-13
GGACCGA6350.020.9525856
GTACTTT58450.020.11619214-15
CATGGGA44550.019.952284
AGTACTT58350.019.94692812-13
GTATAGG4600.019.709771
Produced by FastQC (version 0.11.5)