##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543537 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.985923931910563 31.0 31.0 34.0 28.0 34.0 2 31.149810283868486 31.0 31.0 34.0 27.0 34.0 3 31.209644286676387 31.0 31.0 34.0 27.0 34.0 4 34.878862780451 37.0 35.0 37.0 32.0 37.0 5 34.85931559084849 37.0 35.0 37.0 32.0 37.0 6 34.82645033274531 37.0 35.0 37.0 32.0 37.0 7 34.73108274029432 37.0 35.0 37.0 31.0 37.0 8 34.68324934923298 37.0 35.0 37.0 30.0 37.0 9 36.145949518328216 39.0 35.0 39.0 31.0 39.0 10-11 35.96665666746739 38.0 35.0 39.0 30.0 39.0 12-13 35.76856263541674 38.0 35.0 39.0 30.0 39.0 14-15 36.915623008432746 39.0 36.0 41.0 31.0 41.0 16-17 36.83637647103227 39.0 36.0 41.0 30.5 41.0 18-19 36.78608921356363 39.0 36.0 41.0 30.0 41.0 20-21 36.70029451114727 39.0 36.0 41.0 30.0 41.0 22-23 36.53994693216572 39.0 36.0 41.0 30.0 41.0 24-25 36.3840126957068 39.0 36.0 41.0 29.0 41.0 26-27 36.16438133197984 39.0 35.0 40.5 28.5 41.0 28-29 35.912303025275435 38.5 35.0 40.0 27.0 41.0 30-31 35.64170405227052 38.0 34.5 40.0 27.0 41.0 32-33 35.365792791691256 38.0 34.0 40.0 26.5 41.0 34-35 35.051981355097254 38.0 34.0 40.0 25.0 41.0 36-37 34.85777148120904 38.0 34.0 40.0 25.0 41.0 38-39 34.62805160687657 38.0 33.0 40.0 24.0 41.0 40-41 34.338818542840144 38.0 33.0 40.0 24.0 41.0 42-43 33.92930238482869 38.0 33.0 40.0 23.0 41.0 44-45 33.45881935273597 37.5 32.0 40.0 20.5 41.0 46-47 32.97064540441126 37.0 31.0 40.0 18.0 41.0 48-49 32.490419640052416 37.0 31.0 39.5 14.5 41.0 50-51 31.749073239351343 35.5 30.0 38.5 13.0 40.0 52-53 31.746922887302212 36.0 30.0 39.0 10.5 40.0 54-55 31.706669295551826 36.0 30.0 39.0 8.0 40.0 56-57 31.260421806327173 35.5 29.5 39.0 6.5 40.0 58-59 30.78652679320175 35.0 29.0 39.0 2.0 40.0 60-61 30.218784118336004 35.0 28.0 38.5 2.0 40.0 62-63 29.47753404019678 34.0 27.0 38.0 2.0 40.0 64-65 28.788300504376387 34.0 26.0 37.5 2.0 40.0 66-67 28.085892597591464 33.5 25.5 37.0 2.0 39.0 68-69 27.237183496839243 33.0 23.0 36.0 2.0 39.0 70-71 26.40124126364193 32.0 21.0 35.5 2.0 38.5 72-73 25.508661757230186 31.5 18.5 35.0 2.0 38.0 74-75 24.571446965387175 31.0 10.5 35.0 2.0 37.0 76-77 23.631245191243533 30.0 6.0 34.0 2.0 36.0 78-79 22.651151133244767 29.0 2.0 34.0 2.0 36.0 80-81 21.703806353121657 29.0 2.0 34.0 2.0 35.0 82-83 20.721630744903653 27.5 2.0 33.5 2.0 35.0 84-85 19.756945544727678 26.0 2.0 33.0 2.0 35.0 86-87 18.768194054185173 25.0 2.0 32.5 2.0 34.5 88-89 17.70808425432773 22.0 2.0 32.0 2.0 34.0 90-91 16.573282991362028 18.0 2.0 31.0 2.0 34.0 92-93 15.416060981224177 4.5 2.0 31.0 2.0 34.0 94-95 14.174121312172772 2.0 2.0 30.5 2.0 34.0 96-97 12.780938708577859 2.0 2.0 29.5 2.0 34.0 98-99 11.115759078794607 2.0 2.0 28.0 2.0 33.5 100-101 7.911733542700578 2.0 2.0 9.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5849700941758442 1101 2 0.548146953777259 1101 3 0.4949580753112812 1101 4 0.37050321159173905 1101 5 0.41447114876613256 1101 6 0.5928839120998717 1101 7 0.33535286859797253 1101 8 0.6184205379490066 1101 9 0.6092905676866494 1101 10-11 0.6202255318805356 1101 12-13 0.5556665068733864 1101 14-15 0.7412541119566498 1101 16-17 0.7875732800327739 1101 18-19 0.7790941497665003 1101 20-21 0.9450432894152527 1101 22-23 1.0276294562747594 1101 24-25 0.9373328002865833 1101 26-27 0.9743741692416492 1101 28-29 1.0792482921100301 1101 30-31 0.9869784552226335 1101 32-33 1.1579780738516234 1101 34-35 1.0347621432446914 1101 36-37 1.0018447052864516 1101 38-39 1.108940828404677 1101 40-41 1.253895706446869 1101 42-43 1.3007914415082666 1101 44-45 1.2675143689115629 1101 46-47 1.3529613277811663 1101 48-49 1.2669934001513 1101 50-51 1.2604150712631537 1101 52-53 1.3585064034619236 1101 54-55 1.4723138862836045 1101 56-57 1.7151948194519306 1101 58-59 1.6490713604688843 1101 60-61 1.7068911759931602 1101 62-63 1.6982739055201925 1101 64-65 1.5922729414273427 1101 66-67 1.8401610525260175 1101 68-69 2.0464444233052106 1101 70-71 2.2918464416841218 1101 72-73 2.3867027621865056 1101 74-75 2.4466783360640854 1101 76-77 2.540727546764547 1101 78-79 2.7374677157564697 1101 80-81 3.0532523734169033 1101 82-83 3.1182648517899487 1101 84-85 3.135758404038313 1101 86-87 3.371549871211826 1101 88-89 3.416074353256988 1101 90-91 3.360333472487955 1101 92-93 3.2626043896101535 1101 94-95 3.335934692078032 1101 96-97 2.680353405787189 1101 98-99 2.684278973685899 1101 100-101 2.1761191281465146 1102 1 0.6297893401207055 1102 2 0.5021338741704469 1102 3 0.46390213945838 1102 4 0.3352822824057071 1102 5 0.4059766649053884 1102 6 0.5034155897216763 1102 7 0.4831670223147384 1102 8 0.6073765366511807 1102 9 0.6398964997201944 1102 10-11 0.666996243968093 1102 12-13 0.6945280425889351 1102 14-15 0.7474929044035505 1102 16-17 0.8301383471816592 1102 18-19 0.8969724611706553 1102 20-21 0.9653545332333664 1102 22-23 0.7850342880300545 1102 24-25 0.8456972746405285 1102 26-27 0.8507221953887623 1102 28-29 0.8121292392508437 1102 30-31 0.7059637382069681 1102 32-33 0.7783760284897241 1102 34-35 0.8133300117618916 1102 36-37 0.8561149390086129 1102 38-39 1.0479999120153778 1102 40-41 1.0311587529651618 1102 42-43 1.2512367562122009 1102 44-45 1.2376747669115815 1102 46-47 1.275577876879602 1102 48-49 1.4991499924047318 1102 50-51 1.4862948665454425 1102 52-53 1.4506471434799693 1102 54-55 1.5131194006833475 1102 56-57 1.7858028767135963 1102 58-59 1.9526359957653732 1102 60-61 1.9938599351397706 1102 62-63 2.0276205453530167 1102 64-65 2.1362258354813193 1102 66-67 2.379852523856993 1102 68-69 2.283669419920731 1102 70-71 2.1600533693227497 1102 72-73 2.2558185840938982 1102 74-75 2.1980551964895376 1102 76-77 2.3560726391892786 1102 78-79 2.7066390507400726 1102 80-81 2.875284749490014 1102 82-83 3.1364085141857423 1102 84-85 3.320296272805102 1102 86-87 3.326141271525387 1102 88-89 3.436111435084321 1102 90-91 3.273420528941127 1102 92-93 3.431338615841824 1102 94-95 3.319659679995995 1102 96-97 2.96551798995719 1102 98-99 2.5503103786583274 1102 100-101 1.8560921052280852 1103 1 0.5416843489231233 1103 2 0.48101920282639554 1103 3 0.5291887190561653 1103 4 0.36509989169611856 1103 5 0.31105455786999414 1103 6 0.3923866009978241 1103 7 0.5484327720358735 1103 8 0.3299904563239977 1103 9 0.5591950931288991 1103 10-11 0.6150084632047026 1103 12-13 0.507027010073493 1103 14-15 0.6360778446900284 1103 16-17 0.7488894380566649 1103 18-19 0.7683535394543597 1103 20-21 0.7200468220848535 1103 22-23 0.7507073695804607 1103 24-25 0.8472652615622422 1103 26-27 0.8776676160840324 1103 28-29 0.9366597589706345 1103 30-31 0.7868120313422295 1103 32-33 0.9266104091348879 1103 34-35 0.9896786205775285 1103 36-37 1.133384120631483 1103 38-39 1.056722422863082 1103 40-41 1.0708292530473997 1103 42-43 1.3729570382630882 1103 44-45 1.3262794955808914 1103 46-47 1.5384825241687352 1103 48-49 1.3853579923702526 1103 50-51 1.5129939200789408 1103 52-53 1.5401590491910184 1103 54-55 1.5242517127781063 1103 56-57 1.6629039109528847 1103 58-59 1.685178943020528 1103 60-61 1.8454088160366986 1103 62-63 2.0856771825059006 1103 64-65 2.061186743549019 1103 66-67 2.299869568442265 1103 68-69 2.4204396469879335 1103 70-71 2.505096505124211 1103 72-73 2.8900430770120984 1103 74-75 2.8758708750658677 1103 76-77 2.865402448682101 1103 78-79 3.168261978257128 1103 80-81 3.158195595463134 1103 82-83 3.241420002940121 1103 84-85 3.220341057039903 1103 86-87 3.3702799597172195 1103 88-89 3.698259562801635 1103 90-91 3.506871012373832 1103 92-93 3.4275684081679536 1103 94-95 3.2452937881289134 1103 96-97 3.129991593834708 1103 98-99 2.875517546147057 1103 100-101 2.1445059891309723 1104 1 0.6806131330808789 1104 2 0.5928168954664876 1104 3 0.564906274742583 1104 4 0.365374738283748 1104 5 0.33996598631882335 1104 6 0.4387353308079156 1104 7 0.571960619721871 1104 8 0.6127717821623122 1104 9 0.6615427593654744 1104 10-11 0.6515845132723896 1104 12-13 0.7061549676305603 1104 14-15 0.7376958244882204 1104 16-17 0.9019696118367477 1104 18-19 0.8368419474152162 1104 20-21 0.7647784843103977 1104 22-23 0.6645424575735959 1104 24-25 0.8534326703315855 1104 26-27 0.8885821676820456 1104 28-29 0.8219545543188858 1104 30-31 0.7157139322264996 1104 32-33 0.8163339171841599 1104 34-35 0.9541918211533726 1104 36-37 1.0774389034519984 1104 38-39 1.0907777226046491 1104 40-41 1.1669221745061478 1104 42-43 1.2024111831422104 1104 44-45 1.2519827362704845 1104 46-47 1.3425925871351296 1104 48-49 1.3953601614115385 1104 50-51 1.4668856599169686 1104 52-53 1.667147041418957 1104 54-55 1.817944296109669 1104 56-57 2.0215070821453125 1104 58-59 2.084573942670847 1104 60-61 2.1052794742799144 1104 62-63 2.135342315640102 1104 64-65 2.1603254449554825 1104 66-67 2.4706977030025605 1104 68-69 2.6979065682635586 1104 70-71 2.6483228290418097 1104 72-73 2.8328049659429944 1104 74-75 2.77229966006826 1104 76-77 2.8635979549826267 1104 78-79 3.208298189759603 1104 80-81 3.2571346234420737 1104 82-83 3.488402434043497 1104 84-85 3.3821897998574855 1104 86-87 3.3917149528401396 1104 88-89 3.4422338005373447 1104 90-91 3.6439045882546317 1104 92-93 3.5923517760133663 1104 94-95 3.4536530942113846 1104 96-97 3.318704521219461 1104 98-99 2.957441593888939 1104 100-101 2.02476139543261 1105 1 0.5140909816579651 1105 2 0.5368822308871586 1105 3 0.47453244302592523 1105 4 0.4814841289000782 1105 5 0.464787671881453 1105 6 0.4727344766675472 1105 7 0.5448972579214058 1105 8 0.4598013700395853 1105 9 0.4696780041537636 1105 10-11 0.7092305384240376 1105 12-13 0.7335224432035403 1105 14-15 0.84595021933103 1105 16-17 0.7306352607370314 1105 18-19 0.8490773344760001 1105 20-21 0.8418441994191994 1105 22-23 0.8509623838092253 1105 24-25 0.9041701561011735 1105 26-27 0.8242919949713254 1105 28-29 0.8790398129427359 1105 30-31 1.0220889339638504 1105 32-33 0.8849426476106359 1105 34-35 1.0942814237633058 1105 36-37 1.14768120892343 1105 38-39 1.2077991386581957 1105 40-41 1.386494924269556 1105 42-43 1.3136882367117266 1105 44-45 1.3797697482827829 1105 46-47 1.4528696425999286 1105 48-49 1.3349222281027195 1105 50-51 1.3184963020511091 1105 52-53 1.2980702711309107 1105 54-55 1.6261186310868503 1105 56-57 1.764427098722713 1105 58-59 1.8473396138091154 1105 60-61 2.038082018127259 1105 62-63 1.933509167412634 1105 64-65 2.043324087301894 1105 66-67 2.0401945309324425 1105 68-69 2.176903686171574 1105 70-71 2.404721932447128 1105 72-73 2.384937537120308 1105 74-75 2.624330738900614 1105 76-77 2.9188128862660214 1105 78-79 2.9813198629397206 1105 80-81 3.206265810431848 1105 82-83 3.363172710084985 1105 84-85 3.5369400396106414 1105 86-87 3.4685307276503288 1105 88-89 3.5252634149485615 1105 90-91 3.510299613645092 1105 92-93 3.4161238070858815 1105 94-95 3.241672053979608 1105 96-97 3.1334075495008573 1105 98-99 2.9154226417018467 1105 100-101 2.098563356164898 1106 1 0.24419074352108439 1106 2 0.06327068999354069 1106 3 0.026315442474306394 1106 4 0.042807739431914626 1106 5 -0.036157475911409165 1106 6 -0.23567902044655398 1106 7 0.13199212250628278 1106 8 -0.1523483198443074 1106 9 0.07867944373830227 1106 10-11 0.06558405060669514 1106 12-13 0.2448751481425262 1106 14-15 0.43640523567771794 1106 16-17 -0.09109590694092162 1106 18-19 0.19598273224531937 1106 20-21 0.16419257215051175 1106 22-23 0.10155772601327584 1106 24-25 0.23223902743998792 1106 26-27 0.1721702895345416 1106 28-29 0.2938756061269103 1106 30-31 -0.07690772942900281 1106 32-33 0.13264368491875445 1106 34-35 -0.08152111098181081 1106 36-37 0.05729682639756817 1106 38-39 0.193775802589208 1106 40-41 0.4291653199534906 1106 42-43 -0.12866111143900838 1106 44-45 0.32911352645221825 1106 46-47 0.5236909793954325 1106 48-49 0.37127426033865163 1106 50-51 0.09727285700422073 1106 52-53 0.3525894902572091 1106 54-55 0.2551069193266535 1106 56-57 0.794478425965842 1106 58-59 0.7198489307464335 1106 60-61 0.9537679397977143 1106 62-63 0.9452898796706286 1106 64-65 1.1282318260778403 1106 66-67 1.243956286418335 1106 68-69 1.1996477764752314 1106 70-71 1.12886995402744 1106 72-73 1.3805481496837082 1106 74-75 1.6193645085939998 1106 76-77 1.7189830801390436 1106 78-79 2.0081674418814224 1106 80-81 1.9008729182123325 1106 82-83 2.1525115438869697 1106 84-85 2.081165220231412 1106 86-87 1.8255006040075088 1106 88-89 1.7893683162630225 1106 90-91 1.7539417288925812 1106 92-93 1.926048699789746 1106 94-95 1.8519154715802078 1106 96-97 1.7875406370733415 1106 98-99 1.8881975081702915 1106 100-101 1.3485297210121265 1107 1 0.2583918217361294 1107 2 0.176935746758911 1107 3 0.16912728094338192 1107 4 0.10953612755406539 1107 5 0.2098995150353744 1107 6 0.22440813906592894 1107 7 0.2229861538945812 1107 8 0.24467435470801036 1107 9 0.3270202754937017 1107 10-11 0.324190556488972 1107 12-13 0.15281671555889176 1107 14-15 0.2822498019485593 1107 16-17 0.23279279457925384 1107 18-19 0.24677324448195748 1107 20-21 0.3639953574046686 1107 22-23 0.34917891498893283 1107 24-25 0.29107559269976946 1107 26-27 0.39241536779955055 1107 28-29 0.42090203540109883 1107 30-31 0.41460630522171726 1107 32-33 0.46726927448723643 1107 34-35 0.48995218750829395 1107 36-37 0.44819540996363827 1107 38-39 0.46689838654324234 1107 40-41 0.47392909945212836 1107 42-43 0.8015032660229551 1107 44-45 0.5317236827177254 1107 46-47 0.5470848071029266 1107 48-49 0.3695579630087664 1107 50-51 0.6024868504338201 1107 52-53 0.7408702758537729 1107 54-55 0.9846013985628908 1107 56-57 1.0054207582873858 1107 58-59 0.9348541513528765 1107 60-61 1.165509197578107 1107 62-63 1.358863676855396 1107 64-65 1.463088136325144 1107 66-67 1.4092533245967296 1107 68-69 1.3468639551382715 1107 70-71 1.655179459205062 1107 72-73 1.6246824438143648 1107 74-75 1.8646139036869975 1107 76-77 2.0563280141668905 1107 78-79 2.30937894587899 1107 80-81 2.4688282406959132 1107 82-83 2.478299208854093 1107 84-85 2.443461566982549 1107 86-87 2.207175503875469 1107 88-89 2.6465367857105377 1107 90-91 2.687524005429683 1107 92-93 2.987295267452261 1107 94-95 3.0304427976524497 1107 96-97 2.7906217133485693 1107 98-99 2.4551714595117407 1107 100-101 1.7438251500240023 1108 1 0.2921810935526139 1108 2 0.2193383707342207 1108 3 0.12708281055178006 1108 4 0.20019618597657285 1108 5 0.1796593451091013 1108 6 0.15285402164538198 1108 7 0.1470549718290357 1108 8 0.20725068662184043 1108 9 0.3631492080235006 1108 10-11 0.3093213860596222 1108 12-13 0.26577421535703394 1108 14-15 0.31245505362563364 1108 16-17 0.39510889582561504 1108 18-19 0.3561222261595347 1108 20-21 0.31666553383843876 1108 22-23 0.32952021672193865 1108 24-25 0.45423083606134185 1108 26-27 0.36194602098473894 1108 28-29 0.38828970180622235 1108 30-31 0.45343457023329137 1108 32-33 0.5369904598582877 1108 34-35 0.635322241590174 1108 36-37 0.7462366065968595 1108 38-39 0.6953503418616833 1108 40-41 0.9068476233072928 1108 42-43 0.9865879289755881 1108 44-45 0.9227510038324311 1108 46-47 0.9925335307581094 1108 48-49 1.1128007642252484 1108 50-51 1.0299045078885598 1108 52-53 1.1552006977228046 1108 54-55 1.0666285064416492 1108 56-57 1.3467854269539146 1108 58-59 1.4380896100758456 1108 60-61 1.448716418064766 1108 62-63 1.3892149503018594 1108 64-65 1.6708426789754434 1108 66-67 1.7775183637693495 1108 68-69 1.9375179408217882 1108 70-71 2.048729865353984 1108 72-73 2.0962120632537555 1108 74-75 2.2311818883450485 1108 76-77 2.2712399698027674 1108 78-79 2.598310136915625 1108 80-81 2.5626137584555693 1108 82-83 2.8708961949813308 1108 84-85 2.727762432554883 1108 86-87 2.7597876442468277 1108 88-89 2.748470470020578 1108 90-91 2.8236507986991555 1108 92-93 3.0063146029151895 1108 94-95 3.0602548394798195 1108 96-97 2.8896162609950746 1108 98-99 2.8333176731244745 1108 100-101 2.3206390875120553 1109 1 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-0.681389288949724 2315 90-91 -0.5226871535826341 2315 92-93 -0.58420482769397 2315 94-95 -0.5311460131509111 2315 96-97 -0.4769596694870497 2315 98-99 -0.7620847856899378 2315 100-101 -0.225190644250163 2316 1 -1.099146442617183 2316 2 -0.9482501199046105 2316 3 -0.8823732164047513 2316 4 -0.794326675130435 2316 5 -0.7425260891541399 2316 6 -0.5409221747941615 2316 7 -0.7964017737166955 2316 8 -0.77324629762402 2316 9 -0.7970910833085725 2316 10-11 -0.9387872810492155 2316 12-13 -0.8791419318114535 2316 14-15 -1.0316116639899775 2316 16-17 -0.9679074244111874 2316 18-19 -0.9046024243824746 2316 20-21 -1.1080640963380262 2316 22-23 -1.0899869555275572 2316 24-25 -0.9888789186212819 2316 26-27 -1.2292416860909725 2316 28-29 -1.0442482109139988 2316 30-31 -0.9124040231066814 2316 32-33 -1.2298736275882547 2316 34-35 -0.9638058378820205 2316 36-37 -1.3196739349128919 2316 38-39 -1.4279085519546513 2316 40-41 -1.2197307930577779 2316 42-43 -1.0323048087090072 2316 44-45 -1.1600028426470317 2316 46-47 -0.7656614588544741 2316 48-49 -0.6420239578951872 2316 50-51 -0.5008436128272855 2316 52-53 -0.5683513055167069 2316 54-55 -0.6963894840432907 2316 56-57 -0.8840217006961382 2316 58-59 -0.5481426365251956 2316 60-61 -0.6982761917884694 2316 62-63 -0.5385931639420924 2316 64-65 -0.42092693745143706 2316 66-67 -0.27891332830931503 2316 68-69 -0.3604728144185181 2316 70-71 -0.3895867871729486 2316 72-73 -0.2746120295273826 2316 74-75 -0.2484490531164525 2316 76-77 -0.24491968092922178 2316 78-79 -0.20677936415957987 2316 80-81 -0.04055453651177743 2316 82-83 -0.08400927279906867 2316 84-85 0.2403601197584635 2316 86-87 0.2820861581339855 2316 88-89 0.0067455932938145224 2316 90-91 -0.30241226324053017 2316 92-93 -0.20058477139200548 2316 94-95 -0.20832322855563312 2316 96-97 -2.870380127486527E-4 2316 98-99 -0.13360695125456878 2316 100-101 0.01488971092261071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8822.0 3 3776.0 4 4178.0 5 4890.0 6 5755.0 7 7340.0 8 9254.0 9 11952.0 10 14672.0 11 17987.0 12 21667.0 13 25095.0 14 28098.0 15 31581.0 16 36314.0 17 41140.0 18 42437.0 19 43439.0 20 48141.0 21 52750.0 22 59009.0 23 65037.0 24 71527.0 25 78917.0 26 86791.0 27 96053.0 28 105371.0 29 116880.0 30 128352.0 31 143294.0 32 160471.0 33 179757.0 34 201377.0 35 217412.0 36 208246.0 37 135997.0 38 29424.0 39 334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61542347701572 17.399603101303892 13.397067622759945 24.58790579892045 2 16.18094175509082 19.006608165998955 38.99958431045284 25.81286576845738 3 19.647608971812648 22.590742656226034 28.61403350074529 29.147614871216028 4 13.671876075514861 15.20067558558158 34.39035835220995 36.737089986693604 5 15.163016710019244 36.1409045346325 32.75027655955196 15.945802195796293 6 34.93851165275281 33.58046943008565 16.432491840693196 15.048527076468352 7 31.042137236570987 31.13424865284104 19.137424962823093 18.686189147764882 8 27.735680899632964 34.302597237102304 18.60991414911341 19.351807714151317 9 27.445849035677455 16.226961701618887 18.820277084921265 37.50691217778239 10-11 26.576538043808505 25.349501108810962 26.02833687008338 22.04562397729715 12-13 26.88852607748988 22.429262428196672 26.701114304664173 23.98109718964927 14-15 23.53848912715192 24.15576943647438 24.47824250604047 27.827498930333235 16-17 24.08805997235911 27.37435319271175 24.99101936455049 23.546567470378648 18-19 24.016977047362303 25.88911239182884 26.898041367051473 23.195869193757385 20-21 24.374398270795446 25.246964610457027 26.903883001818556 23.474754116928963 22-23 24.243341904295207 24.86905620574084 26.952987657972216 23.934614231991738 24-25 24.08018652712793 25.257544625860184 26.901653194214138 23.76061565279775 26-27 23.767011468078437 25.64227964586177 26.786826045386984 23.803882840672802 28-29 23.72770172030891 25.589904752391217 27.145332536817552 23.537060990482317 30-31 23.689394158383543 25.36973444641547 26.96598987796094 23.97488151724005 32-33 23.613389610044862 25.460374765145012 26.860115404619133 24.06612022019099 34-35 24.269480248147534 25.413708678537095 26.49839554358519 23.818415529730178 36-37 24.270108876048685 25.185294575704326 26.598433638467334 23.946162909779652 38-39 23.75982674841656 25.417375686778755 26.77737702371789 24.04542054108679 40-41 23.821176764799212 25.371255740323285 26.893279432587303 23.9142880622902 42-43 24.088219802801266 25.202026368267482 26.58789491306226 24.12185891586899 44-45 23.816527389878413 25.522351850402845 26.54326715703525 24.11785360268349 46-47 23.952313850583426 25.795557254306793 26.15689579950331 24.095233095606467 48-49 24.204993925743267 25.60220077035613 26.147635404609986 24.045169899290613 50-51 23.988359871991623 25.79365313409385 26.16948803332401 24.048498960590514 52-53 24.28928920171752 25.67765356769399 26.00018519126503 24.032872039323458 54-55 23.904118206767482 25.567816335999122 26.24651049407472 24.28155496315868 56-57 23.920772155168653 25.936894370081976 26.243894306778966 23.898439167970405 58-59 24.152539957227194 25.32351381800661 26.188229045050626 24.335717179715573 60-61 23.900657437147146 25.419140263611812 26.636623743149322 24.04357855609172 62-63 23.800757377073676 25.66826868445152 26.333205019874168 24.197768918600644 64-65 23.676045348414085 25.494515259156685 26.372364746127637 24.457074646301596 66-67 23.745659699185182 25.614728921269997 26.31147706950988 24.328134310034937 68-69 23.657183769982222 25.3748939405973 26.490296793329836 24.477625496090642 70-71 23.70112060799639 25.558288799071082 26.276230519905646 24.464360073026885 72-73 23.93034939331254 25.41509108274219 26.067684608237457 24.586874915707817 74-75 23.686953622307406 25.735473082033185 26.06434659587996 24.51322669977945 76-77 23.942244046974288 25.543668758820655 25.991967811483214 24.52211938272185 78-79 24.039680977865867 25.495741908899518 25.954214830963053 24.51036228227156 80-81 23.797241096751133 25.853128488391068 25.96263374090782 24.38699667394998 82-83 24.000468697716318 25.725115080624267 25.742710905125282 24.53170531653414 84-85 23.94630403832415 25.314967580249252 25.823920904536802 24.9148074768898 86-87 24.09059965389877 25.229474394386735 25.91251616144917 24.767409790265326 88-89 24.01649801708038 25.334420460749868 25.808960622171384 24.840120899998368 90-91 23.969666659260362 25.40284563340325 25.808311329070577 24.819176378265812 92-93 24.18989766414423 24.926968259232748 25.8794527539341 25.00368132268892 94-95 24.000013370119515 24.90973203134585 25.939073938333863 25.151180660200772 96-97 24.092097186388997 24.916978990818436 25.730869973665282 25.260053849127285 98-99 24.19660552971238 24.888404464489863 25.384212050489445 25.53077795530831 100-101 25.303066535585334 23.88140485335522 25.341721751333203 25.473806859726245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 553.0 1 419.5 2 360.0 3 459.5 4 426.0 5 403.5 6 653.5 7 1259.5 8 1977.0 9 2234.5 10 1927.0 11 1619.5 12 1591.5 13 1694.0 14 1823.0 15 1921.0 16 2053.0 17 2160.5 18 2276.5 19 2460.0 20 2655.5 21 3043.0 22 3574.0 23 4134.5 24 4954.5 25 6191.0 26 7554.5 27 9112.5 28 10830.5 29 12711.5 30 15135.0 31 18118.5 32 21623.5 33 24492.0 34 26820.5 35 29608.0 36 33390.5 37 38098.0 38 43632.0 39 48246.0 40 52747.5 41 61024.5 42 70013.0 43 77399.0 44 84048.0 45 90580.0 46 95907.0 47 100227.5 48 105454.0 49 107473.0 50 106933.0 51 106049.0 52 105236.0 53 106216.0 54 106337.0 55 102481.5 56 95619.5 57 88591.0 58 81417.0 59 72886.0 60 63010.0 61 53499.5 62 45123.5 63 36762.0 64 29636.5 65 24266.5 66 19143.5 67 15209.0 68 11794.5 69 8513.0 70 6452.5 71 5049.5 72 4064.5 73 3309.0 74 2702.0 75 2222.0 76 1594.5 77 980.5 78 602.5 79 410.5 80 250.0 81 134.5 82 80.5 83 50.5 84 31.0 85 20.5 86 18.0 87 14.0 88 8.0 89 7.0 90 5.5 91 2.0 92 1.0 93 2.5 94 3.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38894657321674503 2 0.03043006647829381 3 0.03565900555014533 4 0.04521263107240036 5 0.06247206154264711 6 0.05299706668312669 7 0.037899979438081696 8 0.03888286272226431 9 0.036288050852022205 10-11 0.03729059180188848 12-13 0.0291130028774891 14-15 0.027088263312072914 16-17 0.022232819888210787 18-19 0.027206209306174824 20-21 0.034007761632718535 22-23 0.03314282434263783 24-25 0.041870827906179464 26-27 0.03593421286971646 28-29 0.03129500376837451 30-31 0.034106049961136795 32-33 0.029270264202958318 34-35 0.030489039475344763 36-37 0.03259240970349556 38-39 0.01629620485174778 40-41 0.013524473990352804 42-43 0.01440906894611716 44-45 0.011008292782845305 46-47 0.019362800698397548 48-49 0.016807304159522744 50-51 0.01045787814370304 52-53 0.008669030566490678 54-55 0.007745120279359019 56-57 0.008413480912603199 58-59 0.0038725601396795094 60-61 0.006919498320645621 62-63 0.011244184771049133 64-65 0.006388741347187007 66-67 0.003086253512333416 68-69 0.005936615036463005 70-71 0.014310780617698898 72-73 0.011794599410191398 74-75 0.012187752723864445 76-77 0.005720380713942828 78-79 0.007961354601879194 80-81 0.011008292782845305 82-83 0.012580906037537492 84-85 0.00707675964611484 86-87 0.0024178928790892368 88-89 0.01743634946139962 90-91 0.025889145705370122 92-93 0.011539049756303918 94-95 0.021741378246119478 96-97 0.01989355767185616 98-99 0.012286041052282707 100-101 0.12303732951397994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2543537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.45949483657943 #Duplication Level Percentage of deduplicated Percentage of total 1 73.9261720212872 35.82424951353649 2 13.495805991939633 13.079998815637527 3 4.889975201116029 7.108971840284512 4 2.4400732718805984 4.729788724782934 5 1.3521612536638274 3.27625256450725 6 0.8207422205313668 2.3863652038801506 7 0.5849918269180647 1.984388589118399 8 0.40581942236527946 1.5732643362155125 9 0.30771575663891676 1.342057510797995 >10 1.5677191396864991 13.678772110830717 >50 0.1190001579056932 4.004708476693629 >100 0.08311053133582212 7.768827939664247 >500 0.005075837723544653 1.647381196276729 >1k 0.0015554986572143133 1.3654649362639582 >5k 8.18683503797007E-5 0.2295082415098785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5785 0.22743919195985746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4709 0.18513589540863765 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3053 0.12002970666438112 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2778 0.10921799053837235 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2664 0.10473604276249962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.931533136730466E-5 0.0 18-19 0.0 0.0 0.0 3.931533136730466E-5 0.0 20-21 0.0 0.0 0.0 5.897299705095699E-5 0.0 22-23 0.0 0.0 0.0 1.7691899115287096E-4 0.0 24-25 0.0 0.0 0.0 4.914416420913083E-4 0.0 26-27 0.0 0.0 0.0 0.0010615139469172259 0.0 28-29 0.0 0.0 0.0 0.003754614145577595 0.0 30-31 0.0 0.0 0.0 0.01130315776810009 0.0 32-33 0.0 0.0 0.0 0.02714723630912387 0.0 34-35 0.0 0.0 0.0 0.05335090466543243 0.0 36-37 0.0 0.0 0.0 0.09761996778501748 0.0 38-39 0.0 0.0 0.0 0.16323725583704896 0.0 40-41 0.0 0.0 0.0 0.23663897949980678 0.0 42-43 0.0 0.0 0.0 0.3099227571684626 0.0 44-45 0.0 0.0 0.0 0.4014095332601806 0.0 46-47 0.0 0.0 0.0 0.5151684445714767 0.0 48-49 3.931533136730466E-5 0.0 0.0 0.6362793228484587 0.0 50-51 3.931533136730466E-5 0.0 0.0 0.7691848005356321 0.0 52-53 3.931533136730466E-5 0.0 0.0 0.9017757555718671 0.0 54-55 3.931533136730466E-5 0.0 0.0 1.0427605338550217 0.0 56-57 3.931533136730466E-5 0.0 0.0 1.2108532331159325 0.0 58-59 3.931533136730466E-5 0.0 0.0 1.3936498663082157 0.0 60-61 3.931533136730466E-5 0.0 0.0 1.5909538567750343 1.965766568365233E-5 62-63 7.863066273460932E-5 0.0 0.0 1.7872159909606191 3.931533136730466E-5 64-65 7.863066273460932E-5 0.0 0.0 1.9918522907274396 3.931533136730466E-5 66-67 7.863066273460932E-5 0.0 0.0 2.2338184976275164 3.931533136730466E-5 68-69 7.863066273460932E-5 0.0 0.0 2.4999636333184854 3.931533136730466E-5 70-71 7.863066273460932E-5 0.0 0.0 2.7827391541778237 3.931533136730466E-5 72-73 7.863066273460932E-5 0.0 0.0 3.0660847473419888 3.931533136730466E-5 74-75 7.863066273460932E-5 0.0 0.0 3.3591608850195613 3.931533136730466E-5 76-77 7.863066273460932E-5 0.0 0.0 3.674017716274621 3.931533136730466E-5 78-79 7.863066273460932E-5 0.0 0.0 4.014822666232101 3.931533136730466E-5 80-81 7.863066273460932E-5 0.0 0.0 4.381516761894952 3.931533136730466E-5 82-83 7.863066273460932E-5 0.0 0.0 4.7489185335224136 3.931533136730466E-5 84-85 7.863066273460932E-5 0.0 0.0 5.115239133537275 3.931533136730466E-5 86-87 1.1794599410191398E-4 0.0 0.0 5.5051096170411515 3.931533136730466E-5 88-89 1.1794599410191398E-4 1.965766568365233E-5 0.0 5.9108831520831036 3.931533136730466E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4780 0.0 40.53068 1 GTATCAA 7205 0.0 35.697727 1 GTACATG 9440 0.0 29.672346 1 TACATGG 9745 0.0 28.387764 2 ATCAACG 9175 0.0 27.354353 3 TATCAAC 9205 0.0 27.213028 2 TCAACGC 9275 0.0 27.008179 4 ACATGGG 10045 0.0 26.783361 3 CAACGCA 9495 0.0 26.332335 5 AACGCAG 9885 0.0 25.429178 6 CTATACG 120 1.7034801E-4 23.766685 4 CATGGGG 6590 0.0 22.792936 4 ACGCAGA 11180 0.0 22.268091 7 CGCAGAG 11620 0.0 21.384007 8 GAGTACT 5585 0.0 21.35017 12-13 GGACCGA 635 0.0 20.952585 6 GTACTTT 5845 0.0 20.116192 14-15 CATGGGA 4455 0.0 19.95228 4 AGTACTT 5835 0.0 19.946928 12-13 GTATAGG 460 0.0 19.70977 1 >>END_MODULE