FastQCFastQC Report
Thu 26 May 2016
SRR938556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543537
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.22971948117916116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.1958296655405445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.1332396580037955No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG28800.11322815433783744No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG26300.10339932149601126No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC25870.10170876224721716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.042.0649831
GTATCAA72900.037.0915641
ATCAACG91650.029.3851223
TCAACGC92200.029.2086814
TATCAAC95250.028.3299392
CAACGCA95650.028.1054975
GTACATG90200.027.7647911
AACGCAG98000.027.5284716
TACATGG94200.026.1240882
ACATGGG97450.024.224313
ACGCAGA110850.024.0802177
CGCAGAG114650.023.364958
CATGGGG64000.022.5607454
GCAGAGT123000.021.354039
GAGTACT58750.021.3430212-13
GGACCGA7500.020.8983756
GTACTTT62450.020.23061214-15
AGTACTT61500.019.73220412-13
GCACCGT9650.019.1953986
CAGAGTA117900.019.11534910-11
Produced by FastQC (version 0.11.5)