##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2543537 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806815076800532 31.0 31.0 34.0 30.0 34.0 2 32.05753051754309 33.0 31.0 34.0 30.0 34.0 3 32.12092766883281 33.0 31.0 34.0 30.0 34.0 4 35.77352835834509 37.0 35.0 37.0 35.0 37.0 5 35.65839498304919 37.0 35.0 37.0 33.0 37.0 6 35.691618010667824 37.0 35.0 37.0 33.0 37.0 7 35.60501341242529 37.0 35.0 37.0 33.0 37.0 8 35.580297043054614 37.0 35.0 37.0 33.0 37.0 9 37.12185000650669 39.0 37.0 39.0 33.0 39.0 10-11 37.044459349323404 39.0 37.0 39.0 33.0 39.0 12-13 36.91208069707655 39.0 37.0 39.0 33.0 39.0 14-15 37.94195916945576 40.0 37.0 41.0 33.0 41.0 16-17 38.03236909862133 40.0 37.0 41.0 33.0 41.0 18-19 38.13629249348446 40.0 37.0 41.0 33.0 41.0 20-21 38.080271488089224 40.0 37.0 41.0 33.0 41.0 22-23 37.94379303308739 40.0 37.0 41.0 32.5 41.0 24-25 37.80956184242651 40.0 37.0 41.0 32.0 41.0 26-27 37.59341794516848 40.0 37.0 41.0 32.0 41.0 28-29 37.443154158952666 39.0 36.5 41.0 31.5 41.0 30-31 37.19806218663224 39.0 36.0 41.0 31.0 41.0 32-33 37.12978108830342 39.0 36.0 41.0 31.0 41.0 34-35 36.99642545007208 39.0 36.0 41.0 31.0 41.0 36-37 36.872727622991135 39.0 35.5 41.0 30.0 41.0 38-39 36.66755525868112 39.0 35.0 40.0 30.0 41.0 40-41 36.43832741572071 39.0 35.0 40.0 30.0 41.0 42-43 36.17535384781114 38.0 35.0 40.0 30.0 41.0 44-45 35.886225165979496 38.0 35.0 40.0 29.0 41.0 46-47 35.47247671254635 38.0 34.0 40.0 27.5 41.0 48-49 35.06244513054066 38.0 34.0 40.0 26.5 41.0 50-51 34.64874994938151 38.0 33.0 40.0 26.0 41.0 52-53 34.01444052121121 37.0 33.0 40.0 24.0 41.0 54-55 33.550221994018564 37.0 32.0 39.5 23.0 41.0 56-57 33.12447509118208 36.0 31.5 39.0 22.0 40.0 58-59 32.5449625855649 36.0 31.0 39.0 20.5 40.0 60-61 32.00955578786548 35.0 30.5 39.0 18.5 40.0 62-63 31.460743641629747 35.0 29.5 38.0 15.5 40.0 64-65 30.765308112286156 34.0 29.0 38.0 10.0 40.0 66-67 30.07376755282113 34.0 28.0 37.0 7.5 39.0 68-69 29.323709857572346 34.0 27.5 36.5 4.5 39.0 70-71 28.519939753186215 33.0 26.0 36.0 2.0 39.0 72-73 27.9128210047662 32.5 26.0 35.0 2.0 38.0 74-75 27.175655986132696 32.0 24.5 35.0 2.0 37.0 76-77 26.044540732059332 30.5 23.0 34.0 2.0 36.0 78-79 26.531619944982126 31.5 24.0 34.5 2.0 36.0 80-81 26.14677828551344 32.0 23.5 34.5 2.0 36.0 82-83 25.464999329673603 31.0 21.0 34.0 2.0 35.5 84-85 24.677528182212406 31.0 18.5 34.0 2.0 35.0 86-87 23.743555922323914 30.0 10.5 34.0 2.0 35.0 88-89 22.76137205788632 29.0 4.5 34.0 2.0 35.0 90-91 21.880866486314137 29.0 2.0 34.0 2.0 35.0 92-93 20.772861963478416 28.0 2.0 33.0 2.0 35.0 94-95 19.56667919515226 26.5 2.0 33.0 2.0 34.0 96-97 18.164077031315053 24.5 2.0 32.0 2.0 34.0 98-99 16.396026674666025 10.5 2.0 32.0 2.0 34.0 100-101 12.260437139306408 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.323968853516881 1101 2 0.2660068681969534 1101 3 0.25713209721300956 1101 4 0.16967620997081667 1101 5 0.10906430085545793 1101 6 0.1898623661611154 1101 7 0.28896082358700426 1101 8 0.20215865437089775 1101 9 0.35208106092164115 1101 10-11 0.32811566746399734 1101 12-13 0.40266726622474636 1101 14-15 0.4084174943792007 1101 16-17 0.37593178435936636 1101 18-19 0.48811692466001233 1101 20-21 0.5523806828594928 1101 22-23 0.47351187879734624 1101 24-25 0.352384995222927 1101 26-27 0.5208087279189471 1101 28-29 0.48384352207639125 1101 30-31 0.5930498701257747 1101 32-33 0.6081977369161535 1101 34-35 0.5349981703702511 1101 36-37 0.5566014902001797 1101 38-39 0.6317815362589769 1101 40-41 0.8120085259511143 1101 42-43 1.0218901872882213 1101 44-45 0.7949784927776591 1101 46-47 0.8329570305102791 1101 48-49 1.1075799520248282 1101 50-51 1.0889529102922495 1101 52-53 1.1983069825426043 1101 54-55 1.1026314706455977 1101 56-57 1.23770204885804 1101 58-59 1.2846187211976101 1101 60-61 1.3146618183013175 1101 62-63 1.487172029825249 1101 64-65 1.6036985797159282 1101 66-67 1.7092851004620258 1101 68-69 1.7413398624556287 1101 70-71 1.8729837330172145 1101 72-73 2.0491353491788047 1101 74-75 1.9241411583971946 1101 76-77 1.9009201351375147 1101 78-79 2.048021479542065 1101 80-81 2.211639985468416 1101 82-83 2.398132707716595 1101 84-85 2.697833301147366 1101 86-87 2.4801625984513898 1101 88-89 2.243487768368446 1101 90-91 2.462317617798927 1101 92-93 3.0350633031594434 1101 94-95 2.877235562964355 1101 96-97 3.086483661514116 1101 98-99 2.944139704733008 1101 100-101 2.4283066242162494 1102 1 0.1903600724675627 1102 2 0.2338531499174934 1102 3 0.18035501700417456 1102 4 0.2040939390624672 1102 5 0.19106709768591656 1102 6 0.23222398620512053 1102 7 0.19878157080511016 1102 8 0.30457687657597177 1102 9 0.2604701153161315 1102 10-11 0.3542070023568442 1102 12-13 0.3435598317834945 1102 14-15 0.44689111923937475 1102 16-17 0.3593964129247027 1102 18-19 0.31424887334294027 1102 20-21 0.32382752455117725 1102 22-23 0.35725490468353627 1102 24-25 0.3612544192407299 1102 26-27 0.49096818053278923 1102 28-29 0.36329336026408754 1102 30-31 0.37422965156761023 1102 32-33 0.4689966184864929 1102 34-35 0.5832816894152444 1102 36-37 0.4215332219737036 1102 38-39 0.4595293389924251 1102 40-41 0.5126724139387377 1102 42-43 0.5012113773252125 1102 44-45 0.48397423551463703 1102 46-47 0.6125493194868525 1102 48-49 0.6383087842611275 1102 50-51 0.7854287375240716 1102 52-53 0.7801322358493721 1102 54-55 0.6538509809960615 1102 56-57 0.9920602791253685 1102 58-59 1.093482246005678 1102 60-61 1.1536090438197917 1102 62-63 1.1893466449207217 1102 64-65 1.1760711057348452 1102 66-67 1.314068300722802 1102 68-69 1.5278387646732021 1102 70-71 1.690895927061888 1102 72-73 1.8036386016853996 1102 74-75 1.7290333049607902 1102 76-77 1.6037913107668231 1102 78-79 1.8513221630184553 1102 80-81 1.9479653525996845 1102 82-83 2.022911203735763 1102 84-85 2.020023934866188 1102 86-87 2.0251845351921958 1102 88-89 2.0415426930362983 1102 90-91 2.3551233046371074 1102 92-93 2.3553692149995378 1102 94-95 2.3754302535243355 1102 96-97 2.4787656044112794 1102 98-99 2.1756044633834826 1102 100-101 1.9136202390628956 1103 1 0.25382224217934635 1103 2 0.2845857510447516 1103 3 0.25913073741264014 1103 4 0.17716916867593824 1103 5 0.15649381207455093 1103 6 0.1987169747968096 1103 7 0.204193029104772 1103 8 0.2783463156992241 1103 9 0.2759359395938574 1103 10-11 0.36627737717251563 1103 12-13 0.2978844014849287 1103 14-15 0.47113440214285873 1103 16-17 0.3768364067188372 1103 18-19 0.41636895973664423 1103 20-21 0.45559570813566097 1103 22-23 0.4887400892542004 1103 24-25 0.3745618373344186 1103 26-27 0.43215592164759187 1103 28-29 0.5014604460422447 1103 30-31 0.4908534374192399 1103 32-33 0.514122930140239 1103 34-35 0.6273706372981778 1103 36-37 0.601015470250033 1103 38-39 0.5784409994855437 1103 40-41 0.583838944492939 1103 42-43 0.5472234974403349 1103 44-45 0.6453059398079688 1103 46-47 0.8025357513361655 1103 48-49 0.6442801352797503 1103 50-51 0.6513940478784548 1103 52-53 0.8120317553974914 1103 54-55 0.8694055477310414 1103 56-57 1.0963175826906522 1103 58-59 1.1533535963778547 1103 60-61 1.1149326316439314 1103 62-63 1.222423411635436 1103 64-65 1.2765210310705122 1103 66-67 1.1627370920368847 1103 68-69 1.0741674629998457 1103 70-71 1.4307647060217015 1103 72-73 1.4770683342513315 1103 74-75 1.306002213137667 1103 76-77 1.448465614097234 1103 78-79 1.5414294440659226 1103 80-81 1.544809015756158 1103 82-83 1.6665980272585834 1103 84-85 1.9367832585703724 1103 86-87 1.910674802252764 1103 88-89 1.841209917800004 1103 90-91 2.178301893535071 1103 92-93 2.444321227991633 1103 94-95 2.184631456485622 1103 96-97 1.8892197603667213 1103 98-99 1.8883895543633535 1103 100-101 1.9005875598595185 1104 1 0.20541951134959646 1104 2 0.2652537481918813 1104 3 0.26324617230373093 1104 4 0.18037019386891728 1104 5 0.1754382930638485 1104 6 0.19700775674295556 1104 7 0.16177746038856355 1104 8 0.09188224830082703 1104 9 0.151786536570107 1104 10-11 0.3089250704714317 1104 12-13 0.22226265187946836 1104 14-15 0.3948078830453312 1104 16-17 0.3446996273802725 1104 18-19 0.380059056441425 1104 20-21 0.24503746042370977 1104 22-23 0.3631293706764964 1104 24-25 0.3080614638336172 1104 26-27 0.409158509293583 1104 28-29 0.4235513207638846 1104 30-31 0.49805038062515905 1104 32-33 0.5041421305478266 1104 34-35 0.4972652394598569 1104 36-37 0.5346813223437152 1104 38-39 0.5409893087989488 1104 40-41 0.5842904902389279 1104 42-43 0.493199587983284 1104 44-45 0.5030329635961337 1104 46-47 0.6997754773612499 1104 48-49 0.8397505385085751 1104 50-51 0.80186833139377 1104 52-53 0.6655653838150428 1104 54-55 0.8023994869161228 1104 56-57 0.956636560146471 1104 58-59 0.9595559226577421 1104 60-61 0.8450374213398391 1104 62-63 1.017575114451109 1104 64-65 1.1396171922390614 1104 66-67 1.0879171613178187 1104 68-69 1.047549491648084 1104 70-71 1.2444688913591833 1104 72-73 1.3973243926167882 1104 74-75 1.3197088141519835 1104 76-77 1.4352740759259923 1104 78-79 1.380165278148457 1104 80-81 1.5103598593341552 1104 82-83 1.6010163711313652 1104 84-85 1.910084539419664 1104 86-87 1.8339126166835094 1104 88-89 1.8344137573409505 1104 90-91 2.102920444649275 1104 92-93 2.119900475199163 1104 94-95 1.8988532554857684 1104 96-97 2.078861034046689 1104 98-99 2.3634371330612076 1104 100-101 2.0266553988220526 1105 1 0.12240221627063264 1105 2 0.1516134360515906 1105 3 0.1136428863903518 1105 4 0.1453853434887833 1105 5 0.041175418729885394 1105 6 0.10988701844221538 1105 7 0.11976932710223309 1105 8 0.15320409369889632 1105 9 0.28380413363065315 1105 10-11 0.22118449602412937 1105 12-13 0.22298333281633376 1105 14-15 0.2475233165784303 1105 16-17 0.28664335267053076 1105 18-19 0.2850000089342899 1105 20-21 0.269495757272864 1105 22-23 0.39572213840717296 1105 24-25 0.2742775904081256 1105 26-27 0.28214192936764704 1105 28-29 0.32188792661727916 1105 30-31 0.28040149991546315 1105 32-33 0.34081179175986875 1105 34-35 0.33765818193552377 1105 36-37 0.4498091780596951 1105 38-39 0.5086285551590564 1105 40-41 0.40411599163574863 1105 42-43 0.4017502627668108 1105 44-45 0.6508596596523049 1105 46-47 0.5777279316881092 1105 48-49 0.7185323366004113 1105 50-51 0.7021993265810238 1105 52-53 0.5572487605503298 1105 54-55 0.7511899969870157 1105 56-57 0.7518733061243736 1105 58-59 0.9971095907231273 1105 60-61 1.0558014536910392 1105 62-63 0.9779198156851869 1105 64-65 1.263339219457837 1105 66-67 1.399547068992323 1105 68-69 1.4594668977905663 1105 70-71 1.5134503763504483 1105 72-73 1.598491270291504 1105 74-75 1.591367700686508 1105 76-77 1.6309091785681424 1105 78-79 1.7652126412427656 1105 80-81 1.7432694708140026 1105 82-83 1.9318469847351913 1105 84-85 2.0554074101652304 1105 86-87 1.927327586125248 1105 88-89 2.093517822846948 1105 90-91 2.0852271195793683 1105 92-93 2.2134233177682034 1105 94-95 2.128070620942868 1105 96-97 2.204462707075187 1105 98-99 2.1355886349457514 1105 100-101 2.0351921432837248 1106 1 -0.02795868891859854 1106 2 0.009799223181438776 1106 3 0.022086318790421444 1106 4 -0.008026270056760154 1106 5 0.03853674099500637 1106 6 -0.025504562006346987 1106 7 -0.02312793768714272 1106 8 -0.015422381202554902 1106 9 -0.18936005973111492 1106 10-11 -0.02486589616174939 1106 12-13 0.004396883746686342 1106 14-15 -0.028866824285593395 1106 16-17 -0.19178978737279095 1106 18-19 -0.08134146186367275 1106 20-21 -0.015942239686523862 1106 22-23 0.06506588294146098 1106 24-25 -0.018820812594825043 1106 26-27 -0.25509386650733035 1106 28-29 0.046737241132866814 1106 30-31 0.0798258342757876 1106 32-33 -0.12564723389701982 1106 34-35 -0.15825514700281929 1106 36-37 -0.17054244382737238 1106 38-39 -0.12453217378197223 1106 40-41 -0.0797038181238321 1106 42-43 0.03008939255813914 1106 44-45 0.0860606969604163 1106 46-47 -0.40776606780062963 1106 48-49 -0.150905250010112 1106 50-51 0.1453416234583358 1106 52-53 -0.020636799205881573 1106 54-55 0.0784827371756407 1106 56-57 0.09034553021520964 1106 58-59 0.37148313600277305 1106 60-61 0.41920405301564045 1106 62-63 0.39282624269017674 1106 64-65 0.7417261111660984 1106 66-67 0.4077801135118122 1106 68-69 0.5056008733310797 1106 70-71 0.21422617114910736 1106 72-73 0.6313755182767586 1106 74-75 0.8341336202043159 1106 76-77 0.9210762780071065 1106 78-79 1.1477519607363575 1106 80-81 1.1933380865256602 1106 82-83 1.4159611020815142 1106 84-85 1.3861460717552312 1106 86-87 1.4374685846856643 1106 88-89 1.4457443017846145 1106 90-91 1.5280902446886806 1106 92-93 1.607195096529626 1106 94-95 1.5853531360450503 1106 96-97 1.447744926188026 1106 98-99 1.7165307892100152 1106 100-101 1.8612403828198278 1107 1 0.10728066174230122 1107 2 0.054767271306538134 1107 3 0.04512973028221978 1107 4 0.05971581949371796 1107 5 0.07933388828811871 1107 6 -0.05182802798937303 1107 7 -0.0053567141401131835 1107 8 0.01772953513842168 1107 9 0.03702645031000884 1107 10-11 0.08190707836418198 1107 12-13 0.10273275574226659 1107 14-15 0.1993652307523064 1107 16-17 0.10175767532430768 1107 18-19 0.10262991701568325 1107 20-21 0.06434282526429058 1107 22-23 0.1726742279761524 1107 24-25 0.12593811736088156 1107 26-27 0.10887226604830857 1107 28-29 0.1337265052735077 1107 30-31 0.03380778662829442 1107 32-33 0.1482231348813272 1107 34-35 0.28334680398300094 1107 36-37 0.16427573137779916 1107 38-39 0.20695186718049996 1107 40-41 0.19178564550937693 1107 42-43 0.2288729655397148 1107 44-45 0.32395627454464915 1107 46-47 0.3961126113308282 1107 48-49 0.42868610986785427 1107 50-51 0.33343342383645336 1107 52-53 0.337853758513603 1107 54-55 0.4893575432857702 1107 56-57 0.49082019759902806 1107 58-59 0.4550448019824245 1107 60-61 0.4929796352730804 1107 62-63 0.8648478334431253 1107 64-65 0.9651497709104113 1107 66-67 0.9260813333188125 1107 68-69 1.0364866570429392 1107 70-71 0.7223564298722209 1107 72-73 1.0319985375874907 1107 74-75 1.2078899512458712 1107 76-77 1.2622196532742898 1107 78-79 1.2877831741581254 1107 80-81 1.3576904190455927 1107 82-83 1.5973940391484085 1107 84-85 1.8752967624844494 1107 86-87 2.0574118891201287 1107 88-89 1.9475467887184017 1107 90-91 1.9113012005409438 1107 92-93 1.9037132716613137 1107 94-95 1.8901851177519866 1107 96-97 1.824624656788565 1107 98-99 1.894186079289831 1107 100-101 1.5620933145522233 1108 1 0.03301299611007025 1108 2 0.024188822215066352 1108 3 0.040065203980013564 1108 4 0.06256006961051952 1108 5 0.0034646757636451753 1108 6 -0.015417817230826358 1108 7 -0.02302313996747074 1108 8 0.09538841144707533 1108 9 0.08338408357040095 1108 10-11 0.022460139266165413 1108 12-13 0.11710168429718948 1108 14-15 0.21750353600267402 1108 16-17 0.11957674772722982 1108 18-19 -0.01414968189370569 1108 20-21 -5.795066613245581E-5 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2144.0 10 3527.0 11 5372.0 12 7752.0 13 11073.0 14 14744.0 15 19336.0 16 24023.0 17 30015.0 18 31324.0 19 30586.0 20 33715.0 21 36625.0 22 40436.0 23 44788.0 24 50557.0 25 57231.0 26 65681.0 27 76622.0 28 90330.0 29 104677.0 30 121270.0 31 139490.0 32 163418.0 33 193788.0 34 233042.0 35 277789.0 36 308731.0 37 251816.0 38 70297.0 39 1009.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.291001554114 18.20983527650016 14.128005197158553 24.37115797222729 2 16.62493492522102 19.700003302889087 38.071930633038015 25.603131138851886 3 19.74175711628518 22.842684697485392 29.05731754352374 28.358240642705688 4 14.15847302398196 15.91657601206509 34.150987384889625 35.77396357906333 5 15.077247036399836 36.280001635523575 32.93378331199814 15.708968016078456 6 34.84623970478904 33.963256677610744 16.217967342326848 14.972536275273368 7 30.877042480608697 31.26705056777236 19.084369521654295 18.771537429964653 8 27.63557204003716 34.68433917021848 18.518071488639638 19.162017301104722 9 27.16654695110612 16.230402720000438 18.758189900724545 37.8448604281689 10-11 26.502661451356907 25.435151916406166 26.100544242132116 21.961642390104803 12-13 26.72711267813285 22.457664268300405 26.86375311229992 23.951469941266826 14-15 23.36154339410042 24.25236982988649 24.464436727281736 27.921650048731355 16-17 23.910326446990943 27.48234053603309 25.12288989702135 23.484443119954616 18-19 23.884162093966 25.921462907754044 27.067111663797306 23.12726333448265 20-21 24.254099704466654 25.279718753845533 26.996226121342055 23.469955420345762 22-23 24.143289443007905 24.90791366510493 27.132473402195444 23.816323489691715 24-25 24.078886635649017 25.345783641268838 27.02125183507123 23.554077888010923 26-27 23.70051118082732 25.650653651693233 26.951823340058123 23.697011827421328 28-29 23.66842177168957 25.643312990889637 27.230161202972635 23.458104034448155 30-31 23.619314364210155 25.374409729443688 27.066010834519016 23.940265071827145 32-33 23.543839377526385 25.47145610066642 26.979164188974416 24.00554033283278 34-35 24.14251509508038 25.489232375868237 26.58898661205511 23.779265916996277 36-37 24.2426756248893 25.131879738652547 26.761065071286318 23.864379565171838 38-39 23.7342560417578 25.431521829951 26.891059132306754 23.943162995984448 40-41 23.761219218110465 25.34121384306965 27.009383515846835 23.88818342297305 42-43 24.02413046350117 25.22735100400091 26.68571972270451 24.062798809793414 44-45 23.785738829858754 25.43954799683274 26.713038062969378 24.061675110339127 46-47 23.907441469780363 25.805173230264238 26.232825831278618 24.054559468676782 48-49 24.074232063461235 25.590683367295224 26.30087551311422 24.03420905612932 50-51 23.947695669455566 25.72618365685264 26.289611670677488 24.036509003014306 52-53 24.253175794179523 25.69907573587489 26.07441920443854 23.973329265507047 54-55 23.8502526206617 25.536546156002448 26.309367624689557 24.303833598646293 56-57 23.84722534014642 25.89883693455216 26.292501347532983 23.96143637776844 58-59 24.043959258308412 25.370674772963792 26.239229073530286 24.346136895197514 60-61 23.824677997607267 25.485790063207258 26.65158792657626 24.037944012609213 62-63 23.701601352761923 25.693119463172735 26.334568752096 24.270710431969338 64-65 23.546550943253802 25.536457907417077 26.365343364513024 24.5516477848161 66-67 23.661294717047745 25.647557067545346 26.28114727576458 24.41000093964233 68-69 23.482213134154193 25.429643177566714 26.468583128956645 24.619560559322448 70-71 23.48587812954952 25.631728573242697 26.28799974209143 24.59439355511636 72-73 23.729488917680854 25.57340015376247 26.00266518998079 24.694445738575883 74-75 23.476462107687052 25.81206799822452 25.923074836340103 24.788395057748325 76-77 23.53620175369967 25.65386310480249 26.169149495368067 24.64078564612978 78-79 23.593779056487087 25.618675883228747 26.142827881017656 24.64471717926651 80-81 23.312493537527164 25.99490629365241 26.18177250444362 24.510827664376805 82-83 23.50860563103964 25.843568864902778 25.964563129125946 24.68326237493164 84-85 23.3434360531648 25.480557424791357 26.06517443656056 25.11083208548328 86-87 23.463366024822047 25.36969349788051 26.169874513672415 24.997065963625026 88-89 23.244147814637646 25.597366973627665 25.9454452598881 25.213039951846582 90-91 23.151872783197803 25.627791305722177 26.12332885328704 25.097007057792993 92-93 23.233338287086507 25.19885780605349 26.180310325115606 25.387493581744398 94-95 22.998643227914513 25.190885762621107 26.20848448440105 25.601986525063325 96-97 23.053984088689113 25.205535678375362 26.095300775121565 25.645179457813956 98-99 23.05061809598209 25.241641855416297 25.846803093487537 25.860936955114077 100-101 23.275500435417637 24.722797209395587 25.93428044041069 26.06742191477609 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 710.0 1 481.5 2 395.0 3 527.0 4 435.5 5 416.0 6 730.5 7 1435.5 8 2121.0 9 2337.5 10 1991.0 11 1619.0 12 1605.5 13 1706.0 14 1853.0 15 1984.5 16 2130.0 17 2231.0 18 2344.5 19 2488.0 20 2683.5 21 2989.5 22 3537.5 23 4141.0 24 4897.5 25 6141.0 26 7530.5 27 9047.0 28 10737.0 29 12776.0 30 15355.5 31 18238.5 32 22184.0 33 25092.0 34 26943.0 35 29853.5 36 33784.0 37 38880.5 38 44456.0 39 48843.5 40 53256.0 41 62217.5 42 71787.5 43 79158.5 44 85885.0 45 92400.5 46 97194.5 47 100935.0 48 106734.0 49 108686.5 50 107895.0 51 106703.0 52 105354.5 53 107040.5 54 108184.5 55 103933.5 56 95412.5 57 87849.0 58 80935.5 59 71945.0 60 61442.0 61 51504.0 62 43122.5 63 34673.5 64 27491.0 65 22029.0 66 16997.0 67 13863.5 68 10587.0 69 7200.5 70 5423.0 71 4275.5 72 3542.0 73 2946.0 74 2368.5 75 1925.5 76 1357.5 77 795.0 78 454.0 79 311.0 80 197.0 81 100.0 82 58.0 83 37.0 84 14.0 85 6.5 86 4.0 87 3.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023943036802688538 2 0.01214843739249714 3 0.0015726132546921864 4 0.0 5 3.53837982305742E-4 6 0.0 7 0.0 8 0.0 9 3.145226509384373E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015332979233248817 26-27 0.008256219587133979 28-29 1.76918991152871E-4 30-31 0.0 32-33 2.9486498525478497E-4 34-35 0.009494652525204076 36-37 0.0010418562812335734 38-39 0.011774941744507746 40-41 0.011814257075875051 42-43 0.004442632444505427 44-45 5.897299705095699E-4 46-47 1.965766568365233E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.931533136730466E-5 66-67 6.290453018768746E-4 68-69 0.0012187752723864446 70-71 0.0 72-73 1.3760365978556633E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.35891988203828E-4 82-83 0.0011008292782845306 84-85 0.006388741347187007 86-87 0.004422974778821775 88-89 0.0 90-91 0.0014350095949066202 92-93 4.324686450403513E-4 94-95 0.0 96-97 1.965766568365233E-5 98-99 0.0 100-101 7.863066273460932E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2543537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.05144711310546 #Duplication Level Percentage of deduplicated Percentage of total 1 70.87782925864379 31.931487763375088 2 14.730766036283002 13.272846540382677 3 5.581526134149383 7.543674883291406 4 2.7675446062914473 4.987275578539975 5 1.5670976078863814 3.5300007501383694 6 0.970730575278193 2.623969030393197 7 0.6670568371346273 2.1036313073724244 8 0.4644617180466577 1.6739738021312875 9 0.3338023854424411 1.3534452462589723 >10 1.7925409953163964 14.475407675283625 >50 0.1429121231011543 4.497872629847886 >100 0.09559841214853192 8.392790655584749 >500 0.00603439149654336 1.8023402004579814 >1k 0.0020114638321802438 1.5810713718254141 >5k 8.745494922522798E-5 0.23021256511689536 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5843 0.22971948117916116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4981 0.1958296655405445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 0.1332396580037955 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2880 0.11322815433783744 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2630 0.10339932149601126 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2587 0.10170876224721716 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.931533136730466E-5 0.0 18-19 0.0 0.0 0.0 3.931533136730466E-5 0.0 20-21 0.0 0.0 0.0 5.897299705095699E-5 0.0 22-23 0.0 0.0 0.0 1.7691899115287096E-4 0.0 24-25 0.0 0.0 0.0 4.914416420913083E-4 0.0 26-27 0.0 0.0 0.0 0.0010615139469172259 0.0 28-29 0.0 0.0 0.0 0.003675983482842986 0.0 30-31 0.0 0.0 0.0 0.011440761427885656 0.0 32-33 0.0 0.0 0.0 0.027501074291429613 0.0 34-35 0.0 0.0 0.0 0.05407823829572756 0.0 36-37 0.0 0.0 0.0 0.0986814817319347 0.0 38-39 0.0 0.0 0.0 0.16455431943785367 0.0 40-41 0.0 0.0 0.0 0.23842782707701912 0.0 42-43 0.0 0.0 0.0 0.31202612739661345 0.0 44-45 0.0 0.0 0.0 0.40414194879020826 0.0 46-47 0.0 0.0 0.0 0.5185102477376975 0.0 48-49 0.0 0.0 0.0 0.6403681173106583 0.0 50-51 0.0 0.0 0.0 0.7725855766989039 0.0 52-53 0.0 0.0 0.0 0.9056483157115465 0.0 54-55 0.0 0.0 0.0 1.0463972020064973 0.0 56-57 0.0 0.0 0.0 1.2144702436017245 0.0 58-59 0.0 0.0 0.0 1.3965985161607635 0.0 60-61 0.0 0.0 0.0 1.5929196233433993 0.0 62-63 0.0 0.0 0.0 1.7883954509016382 0.0 64-65 0.0 0.0 0.0 1.9930710659998263 0.0 66-67 0.0 0.0 0.0 2.236079129181136 0.0 68-69 0.0 0.0 0.0 2.5030892021621858 3.931533136730466E-5 70-71 0.0 0.0 0.0 2.788518507888818 3.931533136730466E-5 72-73 0.0 0.0 0.0 3.0763853641602226 3.931533136730466E-5 74-75 0.0 0.0 0.0 3.376774153472114 3.931533136730466E-5 76-77 0.0 0.0 0.0 3.699277816678114 3.931533136730466E-5 78-79 0.0 3.931533136730466E-5 0.0 4.050973113424337 3.931533136730466E-5 80-81 0.0 3.931533136730466E-5 0.0 4.431761755382367 3.931533136730466E-5 82-83 0.0 3.931533136730466E-5 0.0 4.814024722266671 3.931533136730466E-5 84-85 0.0 3.931533136730466E-5 0.0 5.198784212692798 5.897299705095699E-5 86-87 0.0 3.931533136730466E-5 0.0 5.60892174951652 7.863066273460932E-5 88-89 0.0 3.931533136730466E-5 0.0 6.0427664311547264 7.863066273460932E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4790 0.0 42.064983 1 GTATCAA 7290 0.0 37.091564 1 ATCAACG 9165 0.0 29.385122 3 TCAACGC 9220 0.0 29.208681 4 TATCAAC 9525 0.0 28.329939 2 CAACGCA 9565 0.0 28.105497 5 GTACATG 9020 0.0 27.764791 1 AACGCAG 9800 0.0 27.528471 6 TACATGG 9420 0.0 26.124088 2 ACATGGG 9745 0.0 24.22431 3 ACGCAGA 11085 0.0 24.080217 7 CGCAGAG 11465 0.0 23.36495 8 CATGGGG 6400 0.0 22.560745 4 GCAGAGT 12300 0.0 21.35403 9 GAGTACT 5875 0.0 21.34302 12-13 GGACCGA 750 0.0 20.898375 6 GTACTTT 6245 0.0 20.230612 14-15 AGTACTT 6150 0.0 19.732204 12-13 GCACCGT 965 0.0 19.195398 6 CAGAGTA 11790 0.0 19.115349 10-11 >>END_MODULE