FastQCFastQC Report
Thu 26 May 2016
SRR938555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332346
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.28769316387877275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.22132222234608417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.15915305876572344No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36950.15842417891685023No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34570.14821986103262552No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34520.14800548460648635No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29870.12806847697554308No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28810.12352369674139257No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27180.11653502524925548No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25360.10873172333778951No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25000.10718821306958744No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24140.10350093853999363No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23930.10260055755020911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91400.036.262041
GGTATCA57600.035.0858571
ATCAACG114450.028.7108823
TATCAAC116150.028.3725452
TCAACGC116150.028.2906634
CAACGCA117900.027.9514085
AGGTAAG11800.027.4038242
GTACATG100200.027.2876241
AACGCAG121250.027.2376986
TACATGG100000.027.0580522
GTAAGGT13500.025.714224
GTGTAAG8400.025.47411
ACATGGG103350.025.4447943
TAAGGTG14700.025.2325745
AAGGTAA12500.024.3464471
ACGCAGA136250.023.957477
CGCAGAG136850.023.7130438
GGTAAGG14850.023.0563373
TAAGGTA6250.022.8257734
GAGTACT65850.022.72728312-13
Produced by FastQC (version 0.11.5)